1. Variegated expression of the Sgs-4 locus in Drosophila melanogaster
- Author
-
Kornher Js and Kauffman Sa
- Subjects
Male ,Transcription, Genetic ,Locus (genetics) ,Biology ,Salivary Glands ,Translocation, Genetic ,stomatognathic system ,Genetics ,medicine ,RNA, Messenger ,Salivary Proteins and Peptides ,Gene ,Genetics (clinical) ,Salivary gland ,Glue Proteins, Drosophila ,DNA ,biology.organism_classification ,Drosophila melanogaster ,Phenotype ,medicine.anatomical_structure ,Position effect ,Gene Expression Regulation ,Larva ,Instar ,Female ,Developmental biology - Abstract
Experiments on T(1;4)wm258-21 larvae of Drosophila melanogaster are described which establish the existence of a salivary gland specific marker for position-effect variegation. The marker is a glue protein gene called Sgs-4 which is expressed during the third larval instar. Using temperature as a variegation modifier, we showed that cytological compaction for the Sgs-4 chromosomal locus is enhanced at 17 degrees C and reduced at 29 degrees C. We also found that the Sgs-4 protein and transcript from salivary glands at 17 degrees C accumulate to roughly half the levels found in salivary glands at 29 degrees C. Southern analysis suggested that the Sgs-4 locus at 17 degrees C is polytenized to roughly one-third the level at 29 degrees C. The results are discussed with respect to alternative models of variegation.
- Published
- 1986