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The hippoboscids play important roles in ecosystem functioning and can serve as indicators of biodiversity and ecosystem health. These bloodsucking ectoparasites are important from a public health perspective, as they can serve as vectors of various endoparasitic pathogens in animals and humans, although our understanding is still limited in this case. The current study provides information on the host species and distribution, which can contribute to the understanding of the ecology and epidemiology of these important arthropods with a potential impact on both animal and human health. We present an ecological parasite-host interaction network of louse flies and deer keds (Diptera: Hippoboscidae) collected from various sites in Slovakia from this time. A total of 123 new samples of eight hippoboscid fly species were captured on 17 species, including 15 birds and two mammals. New host-parasite interactions were recorded for the first time.

This paper attempts to fill the gaps in knowledge about the biodiversity of some winter-active fly families from snowfields and caves in Vârghiș, Romania. A total of 15 fly species were recorded from caves and 9 species from snowfields. Exechiopsis (Exechiopsis) pseudindecisa Lastovka et Matile, 1974 and Rymosia placida Winnertz, 1863 from caves and Mycetophila mitis (Johannsen, 1912) (all Mycetophilidae) from snow represent the first records for Romania. Habitus photographs of these three species are provided.

The nature conservation depends on the completeness of the knowledge about the biodiversity in the study area. Today, data on distribution and diversity of Diptera taxa remain fragmentary. This paper supplements data on the diversity of Diptera species in Europe, Russia, and the Republic of Mordovia. We have studied 1321 specimens of 241 species collected in 2013–2022. Among them, there are species, which are new for Russia (Neoempheria brevilineata, Desmometopa discipalpis) and for both Russia and Europe (Neophyllomyza flavescensis, Phyllomyza auriculatusa, P. luteipalpis, P. orbita, P. piceus, P. striolatum). We have found for the first time 231 Diptera species for the fauna of the Republic of Mordovia; the families of Bolitophilidae, Keroplatidae, Scatopsidae, Platypezidae, Micropezidae, Opomyzidae, Asteiidae, and Milichiidae have been found for the first time in this region. Anthrax incomptus (Bombyliidae), being previously reported for the Republic of Mordovia, has been excluded now from the fauna of this region. Nine Diptera species have been previously indicated for the Republic of Mordovia without reliable confirmation by collected specimens. In this study, we have confirmed their presence in the Republic of Mordovia. Taking into account the results of the present paper, the Diptera fauna of the Republic of Mordovia includes currently 1187 species belonging to 71 families. Data on collecting methods have been presented for the found species. We provide a comparison of collecting methods and suggestions for an effective inventory of the regional Diptera fauna. We are of the view that the number of the used collecting methods affects positively the results of the fauna inventory.

We have studied the fauna of several Diptera families caught with beer traps. In this paper, 36 species from 12 Diptera families are reported: four species of Anisopodidae, one species of Bibionidae, one species of Clusiidae, two species of Dryomyzidae, 11 species of Lauxaniidae, five species of Limoniidae, one species of Megamerinidae, three species of Pallopteridae, one species of Piophilidae, one species of Platystomatidae, four species of Sciomyzidae, and two species of Ulidiidae. From these species, three are new for Russia (Calliopum splendidum, Homoneura biumbrata, Sapromyza schnabli, all Lauxaniidae), two new for European Russia (Sylvicola fuscatoides (Anisopodidae), Myennis sibirica (Ulidiidae)). For the Republic of Mordovia and the Mordovia State Nature Reserve, the families Anisopodidae, Clusiidae, Megamerinidae, Pallopteridae, Piophilidae, Platystomatidae, Ulidiidae, and all Lauxaniidae species published in this list are recorded for the first time. Two species from the family Limoniidae (Achyrolimonia neonebulosa and Discobola parvispinula) and three species from the family Sciomyzidae (Euthycera chaerophylli, Tetanocera ferruginea and T. freyi) are recorded for the first time for the territory of the Mordovia State Nature Reserve. Beer traps can be recommended as one of the successful methods for future surveys of Diptera in the area of interest.

The current geopolitical situation in Europe raises the question of what attitudes towards Muslims are. This article deals with the attitudes of people in Prague to a foreign woman whose Muslim belief is obvious compared to a stranger, whose particular worldview cannot be determined. A total of one hundred people were approached. Fifty of them were addressed by a figurant serving as a Muslim woman, another fifty by the same figurant in the role of “ordinary” stranger. The responses of individuals were rated on a scale of one to five. The attitude of people to the figurant in the role of the Muslim woman did not differ statistically significantly from the attitude towards figurant in the role of foreigner (p = 0.124). Other aspects of this issue, such as the difference between the evaluation of Czechs and foreigners, are discussed.

L’article décrit le projet de simulation globale – l’organisation des Journées libanaises à Paris - récemment expérimenté dans le cours de français de la diplomatie et des relations internationales à l’Université d’économie de Prague où les étudiants ont chacun tenu à la fois un rôle principal et des rôles secondaires en accomplissant des tâches individuelles ou en groupe, en présentiel comme en autonomie. Sont successivement présentés le public cible, la méthodologie adoptée, les phases et l’évaluation du projet pour essayer de tracer les contours d’un modèle d’enseignement-apprentissage hybride visant à l’autonomisation progressive des étudiants formés.

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Abstract Background Glioblastoma is a malignant and aggressive type of central nevous system malignancy characterized by many distinct biological features including extensive hypoxia. Hypoxia in glioblatoma associates with complex signaling patterns including activation of several pathways such as MAPK, PI3K-AKT/mTOR and IL-6/JAK/STAT3 with the master regulator HIF-1, which in turn drive particular tumor behaviors determining, in the end, treatment outcomes and patients fate. Thus, the present study was designed to investigate the expression of selected hypoxia related factors including STAT3 in a small set of long-term surviving glioma patients. Methods The expression of selected hypoxia related factors including STAT3 was evaluated in a time series of formalin fixed paraffin embedded and cryopreserved glioma samples from repeatedly resected patients. In addition, comparative studies were also conducted on primary glioma cells derived from original patient samples, stabilized glioma cell lines and tumor-xenograft mice model. Obtained data were correlated with clinical findings too. Results Glioblastoma samples of the analyzed patients displayed heterogeneity in the expression of hypoxia- related and EMT markers with most interesting trend being observed in pSTAT3. This heterogeneity was subsequently confirmed in other employed models (primocultures derived from glioblastoma tissue resections, cryopreserved tumor specimens, stabilized glioblastoma cell line in vitro and in vivo) and concerned, in particular, STAT3 expression which remained stable. In addition, subsequent studies on the role of STAT3 in the context of glioblastoma hypoxia demonstrated opposing effects of its deletion on cell viability as well as the expression of hypoxia and EMT markers. Conclusions Our results suport the importance of STAT3 expression and activity in the context of hypoxia in malignant glioblastoma long-term surviving glioma patients while emphasizing heterogeneity of biological outcomes in varying employed tumor models.

Abstract Background Siderophores are small iron-binding molecules produced by microorganisms to facilitate iron acquisition from the environment. Radiolabelled siderophores offer a promising solution for infection imaging, as they can specifically target the pathophysiological mechanisms of pathogens. Gallium-68 can replace the iron in siderophores, enabling molecular imaging with positron emission tomography (PET). Stereospecific interactions play a crucial role in the recognition of receptors, transporters, and iron utilisation. Furthermore, these interactions have an impact on the host environment, affecting pharmacokinetics and biodistribution. This study examines the influence of siderophore stereoisomerism on imaging properties, with a focus on ferrirubin (FR) and ferrirhodin (FRH), two cis–trans isomeric siderophores of the ferrichrome type. Results Tested siderophores were labelled with gallium-68 with high radiochemical purity. The resulting complexes differed in their in vitro characteristics. [68Ga]Ga-FRH showed less hydrophilic properties and higher protein binding values than [68Ga]Ga-FR. The stability studies confirmed the high radiochemical stability of both [68Ga]Ga-siderophores in all examined media. Both siderophores were found to be taken up by S. aureus, K. pneumoniae and P. aeruginosa with similar efficacy. The biodistribution tested in normal mice showed rapid renal clearance with low blood pool retention and fast clearance from examined organs for [68Ga]Ga-FR, whereas [68Ga]Ga-FRH showed moderate retention in blood, resulting in slower pharmacokinetics. PET/CT imaging of mice injected with [68Ga]Ga-FR and [68Ga]Ga-FRH confirmed findings from ex vivo biodistribution studies. In a mouse model of S. aureus myositis, both radiolabeled siderophores showed radiotracer accumulation at the site of infection. Conclusions The 68Ga-complexes of stereoisomers ferrirubin and ferrirhodin revealed different pharmacokinetic profiles. In vitro uptake was not affected by isomerism. Both compounds had uptake with the same bacterial culture with similar efficacy. PET/CT imaging showed that the [68Ga]Ga-complexes accumulate at the site of S. aureus infection, highlighting the potential of [68Ga]Ga-FR as a promising tool for infection imaging. In contrast, retention of the radioactivity in the blood was observed for [68Ga]Ga-FRH. In conclusion, the stereoisomerism of potential radiotracers should be considered, as even minor structural differences can influence their pharmacokinetics and, consequently, the results of PET imaging.

The shift of instruction imposed on higher education institutions by the pandemic-related restrictions bolstered the interest in students’ online class participation. This study investigates university students’ engagement in remote foreign language classes during the COVID-19 lockdown. While engagement is a multi-faceted construct, we only aim to explore its behavioural dimension. The authors felt compelled to acknowledge and comprehend their students’ behaviour in contingency online learning (COL). Through a qualitatively oriented exploratory case study, we sought to answer two research questions related to the extent the use of technical equipment and remote physical environment influenced students’ engagement. The study was conducted with students enrolled in regular, in-person Bachelor’s or Master’s degree courses to qualify as teachers of English at primary or lower-secondary schools. The results indicate that the use of technology did not prove to be a significant obstacle to online learning engagement. Concerning the remote physical environment, the learning process was compromised most significantly by the intimate character of the home-working space. We believe that our findings will help educators to rationalise their expectations and formulate best practice recommendations. Received: 7 January 2022 Accepted: 16 January 2023

In the spring of 2020, the University of South Bohemia was one of the innumerable higher education institutions which was obliged to move its instruction online in order to comply with the COVID-19 regulations. This article explores the evaluation of emergency remote teaching (ERT) measures taken by the English department from the perspective of its students. It presents and discusses findings gathered from a questionnaire completed by 99 respondents. The questionnaire centres around four areas of interest: satisfaction with ERT, comparison of face-to-face (F2F) vs. online learning experiences, perceived stress level during ERT, and acquisition of skills. The results show how students responded to the emergency procedures and which factors they considered essential in terms of satisfaction and stress. Furthermore, they reveal thought-provoking insights on aspects such as integrating elements of online teaching into F2F instruction, developing various skills in lockdown, and motivation for learning. Our findings are analysed in terms of aspects and factors which are viewed as efficient or inefficient by students in a time of crisis. Thus, this research may contribute not only to better preparedness should circumstances requiring ERT return, but also to a more complex re-evaluation of instructional approach at university level.

Histological identification of dispersed glioma cells in small biopsies can be challenging, especially in tumours lacking the IDH1 R132H mutation or alterations in TP53. We postulated that immunohistochemical detection of proteins expressed preferentially in gliomas (EGFR, MEOX2, CD34) or during embryonal development (SOX11, INSM1) can be used to distinguish reactive gliosis from glioma. Tissue microarrays of 46 reactive glioses, 81 glioblastomas, 34 IDH1-mutant diffuse gliomas, and 23 gliomas of other types were analysed. Glial neoplasms were significantly more often (p < 0.001, χ2) positive for EGFR (34.1% vs. 0%), MEOX2 (49.3% vs. 2.3%), SOX11 (70.5% vs. 20.4%), and INSM1 (65.4% vs. 2.3%). In 94.3% (66/70) of the glioblastomas, the expression of at least two markers was observed, while no reactive gliosis showed coexpression of any of the proteins. Compared to IDH1-mutant tumours, glioblastomas showed significantly higher expression of EGFR, MEOX2, and CD34 and significantly lower positivity for SOX11. Non-diffuse gliomas were only rarely positive for any of the five markers tested. Our results indicate that immunohistochemical detection of EGFR, MEOX2, SOX11, and INSM1 can be useful for detection of glioblastoma cells in limited histological samples, especially when used in combination.

Objective: To compare the effects of biliopancreatic diversion (BPD) and laparoscopic gastric banding (LAGB) on insulin sensitivity and secretion with the effects of laparoscopic gastric plication (P). Methods: A total of 52 obese women (age 30-66 years) suffering from type 2 diabetes mellitus (T2DM) were prospectively recruited into three study groups: 16 BPD; 16 LAGB, and 20 P. Euglycemic clamps and mixed meal tolerance tests were performed before, at 1 month and at 6 months after bariatric surgery. Beta cell function derived from the meal test parameters was evaluated using mathematical modeling. Results: Glucose disposal per kilogram of fat free mass (a marker of peripheral insulin sensitivity) increased significantly in all groups, especially after 1 month. Basal insulin secretion decreased significantly after all three types of operations, with the most marked decrease after BPD compared with P and LAGB. Total insulin secretion decreased significantly only following the BPD. Beta cell glucose sensitivity did not change significantly post-surgery in any of the study groups. Conclusion: We documented similar improvement in insulin sensitivity in obese T2DM women after all three study operations during the 6-month postoperative follow-up. Notably, only BPD led to decreased demand on beta cells (decreased integrated insulin secretion), but without increasing the beta cell glucose sensitivity.

Recently, we showed that generation of tumours in syngeneic mice by cells devoid of mitochondrial (mt) DNA (ρ0 cells) is linked to the acquisition of the host mtDNA. However, the mechanism of mtDNA movement between cells remains unresolved. To determine whether the transfer of mtDNA involves whole mitochondria, we injected B16ρ0 mouse melanoma cells into syngeneic C57BL/6Nsu9-DsRed2 mice that express red fluorescent protein in their mitochondria. We document that mtDNA is acquired by transfer of whole mitochondria from the host animal, leading to normalisation of mitochondrial respiration. Additionally, knockdown of key mitochondrial complex I (NDUFV1) and complex II (SDHC) subunits by shRNA in B16ρ0 cells abolished or significantly retarded their ability to form tumours. Collectively, these results show that intact mitochondria with their mtDNA payload are transferred in the developing tumour, and provide functional evidence for an essential role of oxidative phosphorylation in cancer.

Toxoplasma gondii is a common protozoan parasite that infects warm-blooded animals throughout the world, including mice and humans. During infection, both, the parasite and the host, utilize various mechanisms to maximize their own reproductive success. Mice and humans are both the intermediate hosts for Toxoplasma gondii, which forms specialized vacuoles containing reproductive cysts in the formers' tissue. As half of the human population is infected, developing a disease called toxoplasmosis, along with an ever-growing number of couples suffering with idiopathic infertility, it is therefore surprising that there is a lack of research on how Toxoplasma gondii can alter reproductive parameters. In this study, a detailed histometric screening of the testicular function along with the levels of the pituitary luteinizing hormone (LH) were analysed in infected mice. Data on relative testis and epididymis weight, and sperm count were also collected. Based on the results obtained, the level of LH in the urine of Toxoplasma gondii infected mice was lower compared to the control. In direct correlation with the hormone level, testicular function and sperm production was also significantly lower in Toxoplasma gondii positive group using sperm count and histometric analysis as a marker. Not only were the number of leptotene primary spermatocytes and spermatids lowered, but the number of Sertoli cells and the tubule diameter were elevated. In parallel, a pilot epigenetic study on global testicular methylation, and specific methylation of Crem, Creb1 and Hspa1genes essential for successfully ongoing spermatogenesis was performed. Global methylation was elevated in Toxoplasma infected mice, and differences in the DNA methylation of selected genes were detected between the Toxoplasma positive and control group. These findings demonstrate a direct relation between Toxoplasma gondii infection and the decrease of male reproductive fitness in mice, which may contribute to an increase of idiopathic infertility in humans.