1. Oncogenic microRNA-155 and its target PU.1: an integrative gene expression study in six of the most prevalent lymphomas
- Author
-
Marek Trneny, Josef Karban, Nina Dusilkova, Hana Huskova, Tomas Stopka, Katarina Korecka, Jarmila Vargova, and Karina Vargova
- Subjects
Adult ,Male ,medicine.medical_specialty ,Lymphoma ,CD38 ,Biology ,Downregulation and upregulation ,immune system diseases ,hemic and lymphatic diseases ,Internal medicine ,Proto-Oncogene Proteins ,microRNA ,Gene expression ,medicine ,Biomarkers, Tumor ,Prevalence ,Humans ,In patient ,RNA, Neoplasm ,Transcription factor ,Aged ,Aged, 80 and over ,Hematology ,ZAP-70 Protein-Tyrosine Kinase ,Middle Aged ,medicine.disease ,ADP-ribosyl Cyclase 1 ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,Cancer research ,Trans-Activators ,Female - Abstract
The transcription factor PU.1 and its inhibitory microRNA-155 (miR-155) are important regulators of B-cell differentiation. PU.1 downregulation coupled with oncogenic miR-155 upregulation has been reported in lymphoid malignancies; however, these data have not been studied across different subtypes in relation to clinical outcomes. We studied expression of miR-155 and PU.1 in the six most prevalent human B-cell lymphomas (n = 131) including aggressive (DLBCL, HL, MCL) and indolent (B-CLL/SLL, MZL, FL) types. Levels of miR-155 and PU.1 inversely correlated in DLBCL, B-CLL/SLL, and FL tumor tissues. In HL tissues, an exceptionally high level of miR-155 was found in patients with unfavorable responses to first-line therapy and those who had shorter survival times. PU.1 downregulation was noted in B-CLL/SLL samples positive for the adverse prognostic markers CD38 and ZAP-70. Upregulation of miR-155 and downregulation of PU.1 expression are integral aspects of lymphoma biology that could mark aggressive behavior of some, but not all, lymphoma types. more...
- Published
- 2015