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19 results on '"Kasper, Łukasz"'

2. The role of MMP-12 gene polymorphism - 82 A-to-G (rs2276109) in immunopathology of COPD in polish patients: a case control study

Author

Gilowska, Iwona, Majorczyk, Edyta, Kasper, Łukasz, Bogacz, Katarzyna, Szczegielniak, Jan, Kasper, Marta, Kaczmarski, Jacek, Skomudek, Aleksandra, Czerwinski, Marcin, and Sładek, Krzysztof

Subjects
0301 basic medicine, Spirometry, Male, lcsh:Internal medicine, medicine.medical_specialty, Chronic bronchitis, lcsh:QH426-470, SNP, 030105 genetics & heredity, Gastroenterology, Polymorphism, Single Nucleotide, 03 medical and health sciences, FEV1/FVC ratio, Pulmonary Disease, Chronic Obstructive, Internal medicine, Matrix Metalloproteinase 12, Genotype, medicine, Genetics, Humans, COPD, Genetic Predisposition to Disease, lcsh:RC31-1245, Promoter Regions, Genetic, Genetics (clinical), Alleles, Genetic Association Studies, medicine.diagnostic_test, business.industry, Smoking, Case-control study, medicine.disease, respiratory tract diseases, lcsh:Genetics, 030104 developmental biology, Case-Control Studies, Female, ELISA, Gene polymorphism, Poland, business, Research Article, Metalloproteinase 12
Abstract

Background Major symptoms of chronic obstructive pulmonary disease (COPD) are chronic bronchitis and emphysema leading from lung tissue destruction, that is an effect of an imbalance between metalloproteinases (MMPs) and their tissue inhibitors activity. As potential factor involved in this COPD pathogenesis, MMP-12 is considered. We investigated the role of genetic polymorphism and protein level of MMP-12 in the COPD development among Poles. Methods We analyzed − 82 A > G SNP in the promoter region of MMP-12 gene (rs2276109) among 335 smoked COPD patients and 309 healthy individuals, including 110 smokers. Additionally, 60 COPD patients and 61 controls (23 smokers) were tested for serum levels of MMP-12 using ELISA. All subjects were analyzed for lung function using spirometry (FEV1% and FEV1/FVC parameters). Results We observed that -82G allele and -82GG homozygous genotype frequencies of the SNP rs2276109 were significantly lower in COPD patients than in controls (12.5% vs 16.9%, respectively; χ2 = 4.742, p = 0.02 for allele and 0.5% vs 3.9%, respectively; χ2 = 9.0331, p = 0.01 for genotype). Moreover, −82G allele was more frequent in controls smokers than in non-smokers (22.3% vs 14.1%, χ2 = 6.7588, p = 0.01). Serum level of MMP-12 was significantly higher in COPD patients than in controls groups (6.8 ng/ml vs 3.3 ng/ml, respectively; F = 7.433, p

Published
2018

3. Impact of Matrix Metalloproteinase 9 on COPD Development in Polish Patients : genetic Polymorphism, Protein Level, and Their Relationship with Lung Function

Author

Gilowska, Iwona, Kasper, Łukasz, Bogacz, Katarzyna, Szczegielniak, Jan, Szymasek, Teresa, Kasper, Marta, Czerwinski, Marcin, Sładek, Krzysztof, and Majorczyk, Edyta

Subjects
Male, 0301 basic medicine, Spirometry, medicine.medical_specialty, Chronic bronchitis, Article Subject, lcsh:Medicine, Gastroenterology, General Biochemistry, Genetics and Molecular Biology, Pulmonary Disease, Chronic Obstructive, 03 medical and health sciences, FEV1/FVC ratio, 0302 clinical medicine, Internal medicine, Humans, Medicine, SNP, Lung, Aged, TIMP1, Tissue Inhibitor of Metalloproteinase-2, COPD, Tissue Inhibitor of Metalloproteinase-1, General Immunology and Microbiology, medicine.diagnostic_test, business.industry, lcsh:R, General Medicine, Middle Aged, respiratory system, medicine.disease, Respiratory Function Tests, Genotype frequency, respiratory tract diseases, 030104 developmental biology, medicine.anatomical_structure, Matrix Metalloproteinase 9, 030228 respiratory system, Female, Poland, business, Polymorphism, Restriction Fragment Length, Research Article
Abstract

Chronic obstructive pulmonary disease (COPD) is characterized by a decline of lung function and symptoms such as chronic bronchitis and emphysema leading from lung tissue destruction. Increased activity of matrix metalloproteinases (MMPs) and an imbalance between MMPs and their tissue inhibitors (TIMPs) are considered as factors influencing the pathogenesis of COPD. We investigated the role of genetic polymorphism and expression level of MMP-9 and concentration of its complexes with TIMPs in the development of COPD among Polish patients. We analyzed SNP in the promoter region of MMP-9 gene (rs3918242) using PCR-RFLP method among 335 COPD patients and 309 healthy individuals. Additionally, 60 COPD patients and 61 controls were tested for copy number variants (CNV) of MMP-9 (by quantitative real-time PCR) and serum levels of MMP-9 and its complexes with TIMP1 and TIMP2 (using ELISA). All subjects were analyzed for lung function using spirometry (FEV1% and FEV1/FVC parameters). We observed that allele and genotype frequencies of the SNP rs3918242, as well as the number of gene copies, were similar in COPD patient and controls groups. Serum levels of MMP-9 and MMP-9/TIMP1 complex were significantly higher in COPD patients in comparison to controls groups, although independently of analyzed gene polymorphisms. Additionally, the significant inverse relationships between parameters of lung function (FEV1% and FEV1/FVC) and proteins level were found in ridge regression models, especially we found that FEV1% decreased when MMP-9 level increased in controls and patients with COPD group. In conclusion, we found that COPD patients were predisposed to produce more MMP-9 and MMP-9/TIMP1 complex than healthy individuals. This phenomenon is probably associated with the disease-related lung environment but not with genetic features of the MMP-9.

Published
2018

8. Assessment of remodeling in chronic obstructive pulmonary disease using imaging methods

Author

Soja, Jerzy, Łoboda, Piotr, Mikrut, Sławomir, Cmiel, Adam, Gross-Sondej, Iwona, Górka, Karolina, Kasper, Łukasz, Andrychiewicz, Anna, Pulka, Grażyna, Reid, Michal, and Sładek, Krzysztof

Subjects
endobronchial ultrasound, ultrasonografia wewnątrzoskrzelowa, computed tomography, przebudowa ściany oskrzeli, przewlekła obturacyjna choroba płuc, tomografia komputerowa, chronic obstructive pulmonary disease, remodeling
Published
2015

18. Assessment of remodeling in chronic obstructive pulmonary disease using imaging methods.

Author

Soja J, Łoboda P, Mikrut S, Ćmiel A, Gross-Sondej I, Górka K, Kasper Ł, Andrychiewicz A, Pulka G, Reid M, and Sładek K

Subjects
Adult, Asthma diagnostic imaging, Bronchoscopy, Female, Humans, Male, Middle Aged, Pulmonary Disease, Chronic Obstructive diagnostic imaging, Respiratory Function Tests, Tomography, X-Ray Computed, Airway Remodeling, Asthma pathology, Pulmonary Disease, Chronic Obstructive pathology
Abstract

Introduction: While spirometry plays a key role in diagnosing chronic obstructive pulmonary disease (COPD), imaging methods including endobronchial ultrasound (EBUS) and chest computed tomography (CT) appear to be useful for investigating structural changes in the lungs., Objectives: The aim of this study was to evaluate remodeling in COPD patients using EBUS and chest CT., Patients and Methods: The study included 33 patients with COPD, 15 patients with severe asthma, and 15 control subjects. All subjects underwent pulmonary function tests and bronchoscopy with EBUS to measure the total thickness of the bronchial wall and its layers. Additionally, in COPD patients, a chest CT was performed to measure total bronchial wall thickness., Results: The total bronchial wall thickness measured by EBUS in patients with COPD (1.192 ±0.079 mm) was significantly smaller than that in asthmatic patients (1.433 ±0.230 mm, P = 0.001) and significantly greater than in control subjects (1.099 ±0.095 mm, P = 0.04), and was positively correlated with residual volume (RV) / total lung capacity (r = 0.5, P = 0.02), RV (r = 0.6, P = 0.007), and RV (%) (r = 0.5, P = 0.05). The thickness of the bronchial wall layers in patients with COPD were as follows: L1 = 0.135 ±0.018 mm, L2 = 0.151 ±0.026 mm, and L3-5 = 0.906 ±0.065 mm. There was no correlation between the thickness of the bronchial wall layers and forced expiratory volume in 1 second., Conclusions: The results of this study show that EBUS is a useful method for evaluating bronchial wall layers not only in asthma but also in COPD, and suggest that the pattern of remodeling differs in each of these diseases.

Published
2015
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19. [The frequency of hypersensitivity to non-steroidal anti-inflammatory drugs (NSAIDs) in the population of adult asthmatics in Poland based on an epidemiological questionnaire].

Author

Kasper Ł, Sładek K, Bochenek G, Duplaga M, and Szczeklik A

Subjects
Adult, Confidence Intervals, Epidemiologic Studies, Female, Humans, Male, Middle Aged, Odds Ratio, Poland epidemiology, Prevalence, Risk Factors, Surveys and Questionnaires, Anti-Inflammatory Agents, Non-Steroidal adverse effects, Aspirin adverse effects, Asthma, Aspirin-Induced epidemiology, Severity of Illness Index
Abstract

Introduction: Hypersensitivity reactions to drugs account for 25% of all side effects related to drugs, affecting more than 7% of the population. One in four such reactions is caused by acetylic acid and other non-steroidal anti-inflammatory drugs., Material and Methods: Between 1998 and 2000 epidemiological research was carried out in various centers, with the aim of estimating the frequency of allergy-based diseases in Poland. The objective of the study was to evaluate the frequency of hypersensitivity to non-steroidal anti-inflammatory drugs (NSAIDs), based on an epidemiological questionnaire, in the Polish adult population., Results: Bronchial asthma was diagnosed in 582 patients (5.4%). Of that group, 75 patients (12.9%) additionally reported symptoms of hypersensitivity to NSAIDs. Aspirin-induced asthma was diagnosed in 11 patients (14.7%) with clinical manifestations of hypersensitivity responses. Frequency of aspirin-induced asthma with clinical symptoms amounted to 1.9% of asthmatics. In the assessment of severity of the disease, aspirin intolerance was the only statistically significant factor (p = 0.0003; odds ratio 28.6 with assumed 95% confidence interval)., Conclusions: In the population of adults in Poland, the frequency of aspirin-induced asthma amounted to 0.1%. Hypersensitivity to NSAIDs was observed in 12.9% of asthmatics. In asthmatics with symptoms of hypersensitivity to non-steroidal anti-inflammatory drugs, which takes the course of clinically demonstrable aspirin-induced asthma, the risk of severe asthma is 30-fold higher.

Published
2009

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