1. Data from A Novel l-Asparaginase with low l-Glutaminase Coactivity Is Highly Efficacious against Both T- and B-cell Acute Lymphoblastic Leukemias In Vivo
- Author
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Arnon Lavie, Pieter Van Vlierberghe, Yogen Saunthararajah, Bradley J. Merrill, Alexander V. Lyubimov, Kasim K. Kabirov, Michael J. Schlicht, Steven Goossens, Barbara De Moerloose, Veerle Mondelaers, Tim Lammens, Sofie Peirs, Andre Kajdacsy-Balla, Maarten C. Bosland, Dolores L. Mahmud, Annie Oh, Damiano Rondelli, Li Liu, Amanda M. Schalk, Michael Caffrey, Aleksandar Antanasijevic, Jenny Y. Zhang, Ying Su, and Hien Anh Nguyen
- Abstract
Acute lymphoblastic leukemia (ALL) is the most common type of pediatric cancer, although about 4 of every 10 cases occur in adults. The enzyme drug l-asparaginase serves as a cornerstone of ALL therapy and exploits the asparagine dependency of ALL cells. In addition to hydrolyzing the amino acid l-asparagine, all FDA-approved l-asparaginases also have significant l-glutaminase coactivity. Since several reports suggest that l-glutamine depletion correlates with many of the side effects of these drugs, enzyme variants with reduced l-glutaminase coactivity might be clinically beneficial if their antileukemic activity would be preserved. Here we show that novel low l-glutaminase variants developed on the backbone of the FDA-approved Erwinia chrysanthemi l-asparaginase were highly efficacious against both T- and B-cell ALL, while displaying reduced acute toxicity features. These results support the development of a new generation of safer l-asparaginases without l-glutaminase activity for the treatment of human ALL.Significance: A new l-asparaginase–based therapy is less toxic compared with FDA-approved high l-glutaminase enzymes Cancer Res; 78(6); 1549–60. ©2018 AACR.
- Published
- 2023