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278 results on '"Kashiwazaki, Naomi"'

18. Carboxylated ε-poly-L-lysine, a cryoprotective agent, is an effective partner of ethylene glycol for the vitrification of embryos at various preimplantation stages

Author

Kawasaki, Yuki, primary, Kohaya, Natsuki, additional, Shibao, Yurie, additional, Suyama, Ayumi, additional, Kageyama, Atsuko, additional, Fujiwara, Katsuyoshi, additional, Kamoshita, Maki, additional, Matsumura, Kazuaki, additional, Hyon, Suong-Hyu, additional, Ito, Junya, additional, and Kashiwazaki, Naomi, additional

Published
2020
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19. Functional expression of a [DELTA]12 fatty acid desaturase gene from spinach in transgenic pigs

Author

Saeki, Kazuhiro, Matsumoto, Kazuya, Kinoshita, Mikio, Suzuki, Iwane, Tasaka, Yasushi, Kano, Koichiro, Taguchi, Yoshitomo, Mikami, Koji, Hirabayashi, Masumi, Kashiwazaki, Naomi, Hosoi, Yoshihiko, Murata, Norio, and Iritani, Akira

Subjects
Linoleic acids -- Research, Science and technology
Abstract

Linoleic acid (18:2n-6) and [alpha]-linolenic acid (18:3n-3) are polyunsaturated fatty acids that are essential for mammalian nutrition, because mammals lack the desaturases required for synthesis of [DELTA]12 (n-6) and n-3 fatty acids. Many plants can synthesize these fatty acids and, therefore, to examine the effects of a plant desaturase in mammals, we generated transgenic pigs that carried the fatty acid desaturation 2 gene for a [DELTA]12 fatty acid desaturase from spinach. Levels of linoleic acid (18:2n-6) in adipocytes that had differentiated in vitro from cells derived from the transgenic pigs were [approximately equal to]10 times higher than those from wild-type pigs. In addition, the white adipose tissue of transgenic pigs contained [approximately equal to]20% more linoleic acid (18:2n-6) than that of wild-type pigs. These results demonstrate the functional expression of a plant gene for a fatty acid desaturase in mammals, opening up the possibility of modifying the fatty acid composition of products from domestic animals by transgenic technology, using plant genes for fatty acid desaturases.

Published
2004

27. Generation of rat offspring derived from sperm cryopreserved/banked in the National BioResource Project for the rat followed by transportation to another institution

Author

Kashiwazaki, Naomi, Seita, Yasunari, Naoi, Kuniko, Takizawa, Akiko, Kuramoto, Takashi, Ito, Junya, Inomata, Tomo, Serikawa, Tadao, Naomi, Kashiwazaki, Yasunari, Seita, Kuniko, Naoi, Akiko, Takizawa, Takashi, Kuramoto, Junya, Ito, Tomo, Inomata, and Tadao, Serikawa

Subjects
endocrine system, sperm cryopreservation, urogenital system, rat sperm, reproductive and urinary physiology, intrauterine insemination
Abstract

To preserve genetic resources efficiently in rats, sperm cryopreservation is essential. This study aimed to confirm the ability of cryopreserved and transported rat spermatozoa to fertilize through intrauterine insemination. The Komeda miniature rat Ishikawa is a mutant caused by the autosomal recessive mutation mri. The epididymal sperm was frozen with egg yolk medium and banked at the National BioResource Project (NBRP), Kyoto University. The sperm was transported to Azabu University, then thawed at 37℃. The thawed semen was inseminated into the uterine horns of recipients; its motility was around 10%. Seven of 15 inseminated female rats became pregnant and 13 live pups were born. The results indicate that rat spermatozoa cryopreserved at NBRP are capable of restoring genetic resources through intrauterine insemination. We also confirmed the usefulness of assisted reproductive technologies for the rat including sperm cryopreservation and intrauterine insemination.

Published
2009

36. Aromatase inhibitor use during ovarian stimulation suppresses growth of uterine endometrial cancer in xenograft mouse model.

Author

Tai Tai Kawahara, Naoki Okamoto, Seido Takae, Megumi Kashiwagi, Mariko Nakajima, Atsushi Uekawa, Junya Ito, Naomi Kashiwazaki, Yodo Sugishita, Nao Suzuki, Kawahara, Tai, Okamoto, Naoki, Takae, Seido, Kashiwagi, Megumi, Nakajima, Mariko, Uekawa, Atsushi, Ito, Junya, Kashiwazaki, Naomi, Sugishita, Yodo, and Suzuki, Nao

Subjects
ENDOMETRIAL cancer risk factors, AROMATASE inhibitors, OVARIAN physiology, XENOGRAFTS, LABORATORY mice
Abstract

Study Question: Could aromatase inhibitors (AI) be used to reduce risks of uterine endometrial cancer growth or recurrence during ovarian stimulation?Summary Answer: In a xenograft mouse model of endometrial cancer, concomitant AI administration suppressed the growth of endometrial cancer during ovarian stimulation.What Is Known Already: Recurrence and mortality rates of estrogen receptor-positive early breast cancer are reduced by long-term AI administration. Concomitant AI use for ovarian stimulation in patients with breast cancer is recommended for reducing estrogen-related potential risks. However, the efficacy of concomitant AI use for estrogen receptor-positive endometrial cancer have not been demonstrated conclusively by clinical or experimental animal studies.Study Design, Size, Duration: Forty nude mice xenografted with uterine endometrial cancer cells were allocated to four groups. Group 1: no ovarian stimulation (control). Group 2: ovarian stimulation. Group 3: AI administration + ovarian stimulation. Group 4: ovariectomy and ovarian stimulation. Tumor growth was evaluated during the 6-week treatment period.Participants/materials, Setting, Methods: Ishikawa 3-H-12 uterine endometrial cancer cells (estrogen and progesterone receptors-positive) were transplanted into 6-week-old BALB/cSlc-nu/nu nude mice, followed by interventions 2 weeks later.Main Results and the Role Of Chance: Compared to ovarian stimulation alone (Group 2), significant suppressions of tumor growth were observed in other three groups (Groups 1, 3 and 4, all at P < 0.05) and correlated with estrogen levels. AI administration had no apparent impact on embryo development.Limitations, Reasons For Caution: In this study, we examined the growth of endometrial cancer tumors using one endometrial cancer cell line. Clinical endometrial cancer or hyperplasia cells can have diverse origins and AI may not be effective against other cancer cell types.Wider Implications Of the Findings: Concomitant AI use may provide a chance for safer childbirth by for patients with endometrial cancer or hyperplasia.Study Funding/conpeting Interest(s): This study was supported by the Graduate Student Aid from the St. Marianna University School of Medicine. The authors declare no competing interests. [ABSTRACT FROM AUTHOR]

Published
2018
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38. Generation of rats from vitrified oocytes with surrounding cumulus cells via in vitro fertilization with cryopreserved sperm.

Author

Fujiwara, Katsuyoshi, Kamoshita, Maki, Kato, Tsubasa, Ito, Junya, and Kashiwazaki, Naomi

Subjects
FERTILIZATION in vitro, CRYOPRESERVATION of cells, CUMULUS cells (Embryology), EMBRYOS, LABORATORY rats
Abstract

The objective of this study was to evaluate fertility and full-term development of rat vitrified oocytes after in vitro fertilization (IVF) with cryopreserved sperm. Oocytes with or without surrounding cumulus cells were vitrified with 30% ethylene glycol + 0.5 mol/L sucrose + 20% fetal calf serum by using the Cryotop method. The warmed oocytes were co-cultured with sperm. Although the denuded/vitrified oocytes were not fertilized, some of the oocytes vitrified with cumulus cells were fertilized (32.7%) after IVF with fresh sperm. When IVF was performed with cryopreserved sperm, vitrified or fresh oocytes with cumulus cells were fertilized (62.9% or 41.1%, respectively). In addition, to confirm the full-term development of the vitrified oocytes with surrounding cumulus cells after IVF with cryopreserved sperm, 108 vitrified oocytes with two pronuclei (2PN) were transferred into eight pseudopregnant females, and eight pups were obtained from three recipients. The present work demonstrates that vitrified rat oocytes surrounded by cumulus cells can be fertilized in vitro with cryopreserved sperm, and that 2PN embryos derived from cryopreserved gametes can develop to term. To our knowledge, this is the first report of successful generation of rat offspring derived from vitrified oocytes that were fertilized in vitro with cryopreserved sperm. [ABSTRACT FROM AUTHOR]

Published
2017
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