1. Streptococcus pyogenes quinolinate-salvage pathway-structural and functional studies of quinolinate phosphoribosyl transferase and NH 3 -dependent NAD + synthetase.
- Author
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Booth WT, Morris TL, Mysona DP, Shah MJ, Taylor LK, Karlin TW, Clary K, Majorek KA, Offermann LR, and Chruszcz M
- Subjects
- Adenosine Triphosphate chemistry, Adenosine Triphosphate metabolism, Amide Synthases chemistry, Amide Synthases genetics, Apoenzymes chemistry, Apoenzymes genetics, Apoenzymes metabolism, Bacterial Proteins chemistry, Bacterial Proteins genetics, Binding Sites, Catalytic Domain, Cluster Analysis, Computational Biology, Crystallography, X-Ray, Dimerization, Gene Deletion, Nicotinamide-Nucleotide Adenylyltransferase chemistry, Nicotinamide-Nucleotide Adenylyltransferase genetics, Pentosyltransferases chemistry, Pentosyltransferases genetics, Protein Conformation, Protein Interaction Domains and Motifs, Protein Multimerization, Protein Structure, Quaternary, Recombinant Proteins chemistry, Recombinant Proteins metabolism, Structural Homology, Protein, Amide Synthases metabolism, Bacterial Proteins metabolism, Models, Molecular, Nicotinamide-Nucleotide Adenylyltransferase metabolism, Pentosyltransferases metabolism, Quinolinic Acid metabolism, Streptococcus pyogenes metabolism
- Abstract
Streptococcus pyogenes, also known as Group A Strep (GAS), is an obligate human pathogen that is responsible for millions of infections and numerous deaths per year. Infection manifestations can range from simple, acute pharyngitis to more complex, necrotizing fasciitis. To date, most treatments for GAS infections involve the use of common antibiotics including tetracycline and clindamycin. Unfortunately, new strains have been identified that are resistant to these drugs, therefore, new targets must be identified to treat drug-resistant strains. This work is focused on the structural and functional characterization of three proteins: spNadC, spNadD, and spNadE. These enzymes are involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD
+ ). The structures of spNadC and spNadE were determined. SpNadC is suggested to play a role in GAS virulence, while spNadE, functions as an NAD synthetase and is considered to be a new drug target. Determination of the spNadE structure uncovered a putative, NH3 channel, which may provide insight into the mechanistic details of NH3 -dependent NAD+ synthetases in prokaryotes., Enzymes: Quinolinate phosphoribosyltransferase: EC2.4.2.19 and NAD synthetase: EC6.3.1.5., Database: Protein structures for spNadC, spNadCΔ69A , and spNadE are deposited into Protein Data Bank under the accession codes 5HUL, 5HUO & 5HUP, and 5HUH & 5HUJ, respectively., (© 2017 Federation of European Biochemical Societies.)- Published
- 2017
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