Search

Your search keyword '"Karepina, Elizaveta"' showing total 12 results
Start Over Author "Karepina, Elizaveta"
12 results on '"Karepina, Elizaveta"'

5. Environmental science

Author

Tardillo Suarez, Vanessa, Karepina, Elizaveta, Chevallet, Mireille, Gallet, Benoit, Cottet-Rousselle, Cécile, Charbonnier, Peggy, Moriscot, Christine, Michaud-Soret, Isabelle, Bal, Wojciech, Fuchs, Alexandra, Tucoulou, Remi, Jouneau, Pierre-Henri, Veronesi, Giulia, Deniaud, Aurélien, European Synchrotron Radiation Facility (ESRF), Biologie des Métaux (BioMet ), Laboratoire de Chimie et Biologie des Métaux (LCBM - UMR 5249), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), Groupe Microscopie Electronique et Méthodes (IBS-MEM), Institut de biologie structurale (IBS - UMR 5075), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Laboratory of Fundamental and Applied Bioenergetics = Laboratoire de bioénergétique fondamentale et appliquée (LBFA), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes (UGA), Integrated Structural Biology Grenoble (ISBG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-European Molecular Biology Laboratory [Grenoble] (EMBL)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Institute of Biochemistry and Biophysics, Polish Academy of Sciences, INSERM, U1160, Département de Gastroentérologie, Université Paris Diderot - Paris 7 (UPD7), Groupe modélisation et chimie théorique (MCT ), GON, Nathalie, and Integrated Structural Biology Grenoble (ISBG - UMS 3518 )

Subjects
[SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health, [SDE] Environmental Sciences, [SDE]Environmental Sciences, [SDV.EE.SANT] Life Sciences [q-bio]/Ecology, environment/Health, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BC] Life Sciences [q-bio]/Cellular Biology
Abstract

International audience; The impact on human health of long-term exposure to low doses of silver nanoparticles (AgNPs) remainsunderstudied. Cellular studies have shown the intracellular dissolution of AgNPs within endolysosomesfollowed by Ag(I) binding to biomolecular thiolate-containing molecules. However, the precise subcellulardistribution of Ag(I) and the nature of the disrupted physiological pathways remained unknown. Novelimaging approaches enabled us to visualize the trafficking of AgNP-containing lysosomes towards aperinuclear location and a nuclear transfer of Ag(I) species with accumulation in the nucleoli. These Ag(I)species impaired nuclear receptor activity, disrupting critical mechanisms of liver physiology in low doseexposure scenarios, thus justifying further research into defining a framework for the safe use of AgNPs.

Published
2020
Full Text
View/download PDF

7. Nuclear translocation of silver ions and hepatocyte nuclear receptor impairment upon exposure to silver nanoparticles

Author

Tardillo Suárez, Vanessa, primary, Karepina, Elizaveta, additional, Chevallet, Mireille, additional, Gallet, Benoit, additional, Cottet-Rousselle, Cécile, additional, Charbonnier, Peggy, additional, Moriscot, Christine, additional, Michaud-Soret, Isabelle, additional, Bal, Wojciech, additional, Fuchs, Alexandra, additional, Tucoulou, Rémi, additional, Jouneau, Pierre-Henri, additional, Veronesi, Giulia, additional, and Deniaud, Aurélien, additional

Published
2020
Full Text
View/download PDF

8. Visualization of silver nanoparticle intracellular trafficking revealed nuclear translocation of silver ions leading to nuclear receptor impairment

Author

Suárez, Vanessa Tardillo, primary, Karepina, Elizaveta, additional, Chevallet, Mireille, additional, Gallet, Benoit, additional, Cottet-Rousselle, Cécile, additional, Charbonnier, Peggy, additional, Moriscot, Christine, additional, Michaud-Soret, Isabelle, additional, Bal, Wojciech, additional, Fuchs, Alexandra, additional, Tucoulou, Rémi, additional, Jouneau, Pierre-Henri, additional, Veronesi, Giulia, additional, and Deniaud, Aurélien, additional

Published
2019
Full Text
View/download PDF

9. Canalicular domain structure and function in matrix-free hepatic spheroidsElectronic supplementary information (ESI) available. See DOI: 10.1039/c9bm01143a

Author

SharmaThese authors contributed equally to this work., Vikas Raj, Shrivastava, Ananya, Gallet, Benoit, Karepina, Elizaveta, Charbonnier, Peggy, Chevallet, Mireille, Jouneau, Pierre-Henri, and Deniaud, Aurélien

Abstract

Liver is pivotal in organism metabolism. This organ is receiving nutriments from the portal vein and then storing, metabolizing, distributing in the circulation or excreting excess and xenobiotics in bile. Liver architecture and hepatocyte polarization are crucial to achieve these functions. To study these mechanisms in details, relevant cell culture systems are required, which is not the case with standard 2D cell culture. Besides, primary hepatocytes rapidly de-differenciate making them inefficient in forming physiological system. Herein, we used an hepatoma-derived cell line to produce matrix-free hepatic spheroids and developed an integrated structural cell biology methodology by combining light sheet fluorescence microscopy and 3D electron microscopy to study their function and structure. Within these spheroids, hepatocytes polarize and organize to form bile canaliculi active for both organics and inorganics excretion. Besides, live imaging revealed the high dynamic of actin networks in basal membranes compared to their high stability in the apical pole that constitutes bile canaliculi. Finally, the first structure of active bile canaliculi was solved at nm resolution and showed the very high density of microvilli coming from all cells constituting the canaliculus. Therefore, this study is the first comprehensive and in-depth functional and structural study of bile canaliculi in a physiological-relevant context.

Published
2019
Full Text
View/download PDF

12. Canalicular domain structure and function in matrix-free hepatic spheroids.

Author

Sharma VR, Shrivastava A, Gallet B, Karepina E, Charbonnier P, Chevallet M, Jouneau PH, and Deniaud A

Subjects
Cell Culture Techniques, Cell Dedifferentiation, Cell Polarity, Hep G2 Cells, Hepatocytes metabolism, Humans, Microscopy, Fluorescence, Spheroids, Cellular metabolism, Bile Canaliculi metabolism, Hepatocytes cytology, Spheroids, Cellular cytology
Abstract

Liver is pivotal in organism metabolism. This organ is receiving nutriments from the portal vein and then storing, metabolizing, distributing in the circulation or excreting excess and xenobiotics in bile. Liver architecture and hepatocyte polarization are crucial to achieve these functions. To study these mechanisms in details, relevant cell culture systems are required, which is not the case with standard 2D cell culture. Besides, primary hepatocytes rapidly de-differenciate making them inefficient in forming physiological system. Herein, we used an hepatoma-derived cell line to produce matrix-free hepatic spheroids and developed an integrated structural cell biology methodology by combining light sheet fluorescence microscopy and 3D electron microscopy to study their function and structure. Within these spheroids, hepatocytes polarize and organize to form bile canaliculi active for both organics and inorganics excretion. Besides, live imaging revealed the high dynamic of actin networks in basal membranes compared to their high stability in the apical pole that constitutes bile canaliculi. Finally, the first structure of active bile canaliculi was solved at nm resolution and showed the very high density of microvilli coming from all cells constituting the canaliculus. Therefore, this study is the first comprehensive and in-depth functional and structural study of bile canaliculi in a physiological-relevant context.

Published
2019
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results