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The survey of protein adsorption is important due to the significant application of proteins in food, medicine, biotechnology, environment, and separation science. Therefore, in the current investigation, the adsorption of bovine serum albumin (BSA) onto a functionalized mesoporous (MCM-41) using layered double hydroxide (MCM-41@LDH) was investigated under different conditions such as the amount of adsorbent, pH of the solution, contact time, and protein concentration. The optimization process was carried out using central composite design (CCD). After the characterization of the modified mesoporous, more investigations were conducted to assess the adsorption of BSA onto MCM-41@LDH, including adsorption isotherm, kinetics, and thermodynamics. It was revealed that Freundlich isotherm and secondorder kinetics provided the best fit for support of MCM-41@LDH. The values of Gibbs free energy (ΔG°), enthalpy (ΔH°), and entropy (ΔS°) were determined as -3100.47 kJ mol-1 K-1 (at 298.15 K), -3101.12 kJ mol-1, and -0.002 kJ mol-1, respectively. According to CCD analysis and desirability function of 1.0, the optimal conditions for BSA adsorption were determined to be a pH value of 4.50, BSA concentration of 250 mg l-1, support amount of 0.016 g, and contact time of 55 min. The obtained findings can be used for purification, separation, and removal of BSA from complex samples. [ABSTRACT FROM AUTHOR]

The aim of the current investigation is the development of a green, quick, easy, and accurate method for simultaneous preconcentration of fast green FCF (FG) and rhodamine B (Rh B) using a deep eutectic solvent (DES). Then, the high-performance thin layer chromatography (HPTLC) technique was used for the determination of analytes. Decanoic acid and tetrabutylammonium bromide were chosen as the components of DES. HPTLC analysis was performed on an aluminum plate silica gel 60 F254. Methanol-ammonia and ethyl acetate were selected as the mobile phase. Scanning of the plates was accomplished by scanner 3. Effective parameters on the preconcentration process such as concentration of salt, volume of DES, stirrer time, and pH were investigated via central composite design (CCD). Data validation demonstrated good repeatability. The limit of detection for FG and Rh B was obtained as 0.08 and 0.01 µg·mL−1, respectively. The enrichment factor for FG and Rh B was achieved as 7.43 and 10.77, respectively. The linear ranges for FG and Rh B were acquired as 0.10–1.20 and 0.05–1.20 µg·mL−1, respectively. The preconcentration factor for both analytes was 21.66. Finally, the proposed method was successfully used for the quantitation of FG and Rh B in pastille and lipstick. [ABSTRACT FROM AUTHOR]