1. The effect of omental pedicle flap with platelets-rich fibrin on reconstruction of induced-urinary bladder defect in dogs
- Author
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Omar M. Sabeel, Osama H. Al-Hyani, Abdul-Haleem M. Al-Hasan, and Karam H. Al-Mallah
- Subjects
partial cystectomy ,prf ,omentum ,Veterinary medicine ,SF600-1100 - Abstract
The research was planned to evaluate the ability of an omental pedicle flap with platelets-rich fibrin (PRF) to augment bladder defects in dogs. Eighteen adult- male dogs were used. After general anesthesia, a partial cystectomy with a circular shape with a diameter of 3cm. was performed on the dorsal surface of the bladder. Then, the animals (N=9) were divided into two equal groups. In the first group, the bladder defect was closed with a double layer of omentum. In contrast, in the second group, the defect was also reconstructed by a double layer of omentum with topical application of platelets-rich fibrin between both layers. The results were evaluated by studying the gross, cystography, and histopathological changes on 7, 15, and 30 days postoperatively. Monitoring of clinical signs with statistical histopathological scoring analysis was also studied. The clinical signs during the first-week post-operation were represented by loss of appetite, straining during urination, and hematuria. These signs disappeared after the first week of surgical repair, and all animals ultimately returned to their normal body status. The complete closure of the bladder defect represented the gross changes. The histopathological results represented the formation of granulation tissue, angiogenesis, and infiltration of polymorphonuclear and mononuclear inflammatory cells in both groups. The proliferation and maturation of granulation tissue were more in the second group, in addition to lower degree of inflammatory cell infiltration. The results of cystography revealed no sign of urine leakage. In conclusion, adding PRF to omentum can improve the healing of bladder defects in dogs.
- Published
- 2024
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