1. γH2Ax Expression as a Potential Biomarker Differentiating between Low and High Grade Cervical Squamous Intraepithelial Lesions (SIL) and High Risk HPV Related SIL.
- Author
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Leventakos, Konstantinos, Tsiodras, Sotirios, Kelesidis, Theodore, Kefala, Maria, Kottaridi, Christine, Spathis, Aris, Gouloumi, Alina-Roxani, Pouliakis, Abraham, Pappas, Asimakis, Sioulas, Vasileios, Chrelias, Charalambos, Karakitsos, Petros, and Panayiotides, Ioannis
- Subjects
Cervix Uteri ,Epithelial Cells ,Humans ,Papillomaviridae ,Papillomavirus Infections ,Adenocarcinoma ,Cervical Intraepithelial Neoplasia ,Carcinoma ,Squamous Cell ,Neoplasm Proteins ,Histones ,Diagnosis ,Differential ,Virus Latency ,Virus Activation ,Adolescent ,Adult ,Aged ,Aged ,80 and over ,Middle Aged ,Uterine Cervical Neoplasms ,Female ,Young Adult ,Neoplasm Grading ,Human Papillomavirus DNA Tests ,Squamous Intraepithelial Lesions of the Cervix ,Biomarkers ,Tumor ,General Science & Technology - Abstract
BackgroundγH2AX is a protein biomarker for double-stranded DNA breakage; its expression was studied in cervical squamous intraepithelial lesions and carcinomas.MethodsImmunostaining for phospho-γH2AX was performed in sections from histologically confirmed cervical SIL and carcinomas, as well as from normal cervices used as controls. In total, 275 cases were included in the study: 112 low grade SIL (LGSIL), 99 high grade SIL (HGSIL), 24 squamous cell carcinoma (SCC), 12 adenocarcinoma and 28 cervical specimens with no essential lesions. Correlation of histological grading, high risk vs. low risk HPV virus presence, activated vs. non-activated status (by high risk HPV mRNA expression) and γH2AX expression in both basal and surface segments of the squamous epithelium was performed.ResultsGradual increase of both basal and surface γH2AX expression was noted up from normal cervices to LGSIL harboring a low risk HPV type, to LGSIL harboring a high risk virus at a non-activated state (p
- Published
- 2017