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Residual substances that are considered hazardous to the recipient must be removed from final cellular therapeutic products manufactured for clinical purposes. In doing so, quality rules determined by competent authorities (CAs) for the clinical use of tissue- and cell-based products can be met. In our study, we carried out residual substance analyses, and purity determination studies of trypsin and trypsin inhibitor in clinically manufactured bone marrow-derived mesenchymal stromal/stem cell products, using the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) method. Despite being a semiquantitative method, SDS-PAGE has several benefits over other methods for protein analysis, such as simplicity, convenience of use, and affordability. Due to its convenience and adaptability, SDS-PAGE is still a commonly used method in many laboratories, despite its limits in dynamic range and quantitative precision. Our goal in this work was to show that SDS-PAGE may be used effectively for protein measurement, especially where practicality and affordability are the major factors. The results of our study suggest a validated method to guide tissue and cell manufacturing sites for making use of an agreeable, accessible, and cost-effective method for residual substance analyses in clinically manufactured cellular therapies., (© 2024 The Authors. ELECTROPHORESIS published by Wiley‐VCH GmbH.)

Introduction  Intracranial hypotension can occur for many reasons, including trauma, surgery, congenital defects, or spontaneous rupture of the dura mater. Symptoms appear long before cerebrospinal fluid (CSF) leaks are diagnosed. Treatment procedures include a variety of conservative and invasive techniques appropriate to the nature of the etiological cause and the severity of the disease. In this cross-sectional study, we aimed to investigate the clinical and imaging features and treatment options of intracranial hypotension patients and to compare them in terms of different etiologies. Methods The data from intracranial hypotension patients were analyzed retrospectively. Symptomatology, neurological findings, and radiological features were compared between patients with spontaneous intracranial hypotension (SIH) and those with secondary causes. Radiological outcomes of conservative treatment and epidural blood patch (EBP) were also evaluated for both groups. Results Of the 30 patients, 23 were female. In 14 of the patients (46.6%), a possible cause of CSF leakage was detected. Compared to intracranial hypotension patients with a secondary cause, SIH patients complained of posterior neck and shoulder pain more frequently (p=0.014, p=0.006). MRI features did not differ significantly when the two groups were compared (p>0.05). The first and sixth-month follow-up MRIs of patients treated with EBP or a conservative approach showed similar improvement rates (p=0.788). Conclusions There was no significant difference in radiological recovery time between conservative treatment and EBP in patients with intracranial hypotension. Radiological recovery times are similar in patients with secondary intracranial hypotension and SIH., Competing Interests: Human subjects: Consent was obtained or waived by all participants in this study. Bezmialem Foundation University Local Ethics Committee issued approval E-54022451-050.04-149082. Animal subjects: All authors have confirmed that this study did not involve animal subjects or tissue. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work., (Copyright © 2024, Yaman Kula et al.)

Polyneuropathy is a frequently encountered clinical presentation where peripheral nerves are affected due to the same cause and physiopathological processes. We report a case of acute sensorimotor polyneuropathy in a patient with Tangier disease (TD) who was treated with miglustat which is a glycosphingolipid synthesis inhibitor. TD is a very rare genetic disorder caused by mutations in the ATP-binding cassette transporter A1 (ABCA1) gene which encodes the cholesterol efflux regulatory protein. It leads to accumulation of cholesterol esters within various tissues and affects lipid metabolism by deficiency of high-density lipoprotein (HDL) in the blood. Due to the accumulation of cholesterol esters in Schwann cells, it could provoke polyneuropathy in TD. Our case presented to our clinic with quadriparesis and after treated with miglustat therapy his weakness regressed., (Copyright © 2023. Published by Elsevier Inc.)

Background: Child hospitalization is a stressful experience for both children and their parents. Parents should establish effective communication with healthcare professionals to be able to participate in the care of their children in the hospital environment., Aims and Objectives: The purpose of the study was to determine the effect of parental participation in the care of hospitalized children on parent satisfaction and parent and child anxiety., Design: The design used for this study was randomized controlled trial., Methods: The study data were collected between 1 February and 31 May 2016. Frequency, percentage, paired t test, Mann-Whitney U and Wilcoxon were used to analyse the data., Results: The study compared the parents' pretest and posttest scores on the anxiety and found that there was a statistically significant difference between the posttest scores of intervention and control groups. There was also a statistically significant difference parental participation and overall satisfaction subscales of the parent satisfaction as well as their total scores and mean scores. Comparison of the children's posttest scores on the anxiety inventory also found a statistically significant difference., Conclusion: The study concluded that parental participation in the care of hospitalized children increases parent satisfaction with healthcare, and reduces anxiety of both parents and children., (© 2020 John Wiley & Sons Australia, Ltd.)

This study aimed to investigate the bacteriome in microscopically healthy middle ear mucosa using Next-generation sequencing (NGS) technology. A total of 60 middle ear washing fluids of pediatric and adult were obtained from 47 patients (35 children and 12 adults). Both children and adults with normal middle ears harbored diverse bacteriome. Seventeen different genera with a mean relative abundance of more than 1% were detected in all samples. Both in adult and children, the most abundant genus was Propionibacterium followed by Streptococcus, Staphylococcus, and Ralstonia. The species Propionibacterium acnes and Corynebacterium tuberculostearicum were significantly more abundant in the adult group. Although there were differences in the prevalence and relative abundance of some bacteria observed from adult and child groups, no specific genus or species was detected only in children or adults., (Copyright © 2021 Elsevier Inc. All rights reserved.)

Objective: The aim of this study was to evaluate the composition and the diversity of bacteriome in middle ear effusion (MEE) and adenoid specimens of pediatric patients having otitis media with effusion (OME)., Materials and Methods: Sample collection from children with OME followed by next generation sequencing. Seventeen adenoid and 43 middle ear effusion specimens from 25 children having OME were evaluated. Microbiome analysis was performed via Ion 16S rRNA metagenomics kit., Results: Twenty-two different bacterial species were identified from all of the samples analyzed. There were variations in the prevalence and relative abundance of the bacteriome observed between adenoid and MEE samples. MEE microbiome was significantly dominated by Alloicoccus otitis (44%), Turicella otitidis (6%), and Staphylococcus auricularis (3%). Whereas, Rothia mucilaginosa (39%), R. dentocariosa (11%), S. aureus (5%), Veillonella rogosae (2%), Granulicatella elegans (2%), Granulicatella adiacens (2%), Eikenella corrodens (1%), and Prevotella nanceiensis (1%) had significantly higher relative abundance in adenoid samples. Overall, there was no statistically significant difference in alpha diversity of MEE and adenoid samples, whereas adenoid samples constituted a cluster in the beta diversity graph., Conclusion: Bacteriome of MEE is mostly dominated by A. otitis yet accompanied by other bacteria with lower relative abundances suggests that OME is likely to be a polymicrobial process. Despite similarities, significant differences in relative abundances of several predominant species between bacteriome in the MEE and adenoid put the theory that OME in children is originated from the adenoids under question., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Purpose: This study aimed to investigate the effect of smoking on the buccal microbiome and to analyse the descriptive ability of each of the seven hypervariable regions in their 16S rRNA genes., Methodology: Microbiome compositions of 40 buccal swab samples collected from smokers (n =20) and non-smokers (n =20) were determined using 16S rRNA sequencing. Seven different 16S rRNA hypervariable regions (V2, V3, V4, V6-7, V8 and V9) in each sample were amplified using the Ion Torrent 16S Metagenomics kit and were sequenced on the Ion S5 instrument., Results: Seven hypervariable regions in the 16S rRNA gene were successfully sequenced for all samples tested. The data obtained with the V2 region was found to be informative but the consensus data generated according to a number of operational taxonomic unit reads gathered from all seven hypervariable regions gave the most accurate result. At the phylum level, no statistically significant difference was found between smokers and non-smokers whereas relative abundances of Veillonella atypica, Streptococcus australis, Prevotella melaninogenica, Prevotella salivae and Rothia mucilaginosa showed significant increases in the smoker group (P-adj=0.05). Alpha diversity results did not show a significant difference between the two groups; however, beta diversity analysis indicated that samples of smoker and non-smoker groups had a tendency to be clustered within themselves., Conclusion: The results of the current study indicate that smoking is a factor influencing buccal microbiome composition. In addition, sequencing of all seven hypervariable regions yielded more accurate results than those with any of the single variable regions.

Aims and Objectives: This study investigates the relationship between breastfeeding self-efficacy and LATCH scores and affecting factors in Turkish mothers., Background: The benefits of breastfeeding for newborns and mothers are well documented. Type of birth, maternal and neonatal complications; lack of support from healthcare professionals during breastfeeding, hospital routines, time and duration of mother-newborn attachment; and insufficiency of husband and family support are the most significant factors affecting breastfeeding initiation and mothers' breastfeeding self-efficacy in early postpartum period. Therefore, it is important to determine how all these factors affect breastfeeding., Design: This was a cross-sectional, descriptive study., Methods: The sample of the study consisted of 303 postpartum women who were recruited at the obstetrics clinic of maternity hospital in one of the three largest city of Turkey. The data were obtained using Personal Identification Form, Breastfeeding Self-Efficacy Scale-Short Form and LATCH Breastfeeding Assessment Tool to determine breastfeeding success. The mean, standard deviation, frequency, percentages, correlation analysis and Kruskal-Wallis variance analysis were used in data collection., Results: The main finding of the study was a weak positive correlation between average LATCH scores and average Breastfeeding Self-Efficacy Scale-Short Form scores of women in the postpartum period. The women becoming pregnant four times or more got significantly higher average Breastfeeding Self-Efficacy Scale-Short Form and average LATCH scores, and the women married for 11 years or longer and the mothers starting to breastfeed their babies within 30 minutes of their birth got significantly higher average LATCH scores., Conclusions: The results of the present research affirm the beneficial role of high self-efficacy in successful breastfeeding., Relevance to Clinical Practice: It is of great importance that midwives and obstetric nurses routinely evaluate breastfeeding self-efficacy and the capability of breastfeeding for early detection of problems related to breastfeeding., (© 2016 John Wiley & Sons Ltd.)