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Japanese quince fruits (Chaenomeles L.) are a valuable source of bioactive compounds, including polyphenolic compounds, vitamins, macro- and microelements. By using Japanese quince fruit, you can fortify wheat beers and influence the qualitative and sensory values as well as the content of bioactive compounds in the finished product. Sensory evaluation showed that wheat beers with the addition of nonozonated Japanese quince fruits were characterized by a balanced taste and aroma (overall impression), but all wheat beers obtained were characterized by high sensory values, and the ozonation process had a positive effect on the microbiological stability of both fruits and beers with the addition of these fruit. Physico-chemical analysis of wheat beers showed that beers enriched with Japanese quince fruit were characterized by high total acidity, respectively: average values of 4.95 (fruit-free) and 6.66 (0.1 M NaOH/100 mL), higher energy value, and, especially beers enriched with ozonated Japanese quince fruit and high total polyphenol content. It was shown that wheat beers enriched with quince fruit contained mainly chlorogenic acid at an average level of 4.35 and 5.42 mg/L (for ozonated and nonozonated fruit, respectively). Electrical properties (impedance and capacity) allowed the differentiation of the analyzed wheat beers. Based on the conducted research, it appears that wheat beers enriched with Japanese quince fruit may constitute a new trend in the brewing industry.

In food authentication, it is important to compare different analytical procedures and select the best method. The aim of this study was to determine the fingerprints of Zweigelt and Rondo wines through headspace analysis using ultra-fast gas chromatography (ultra-fast GC) and to compare the effectiveness of this approach at classifying wines based on grape variety and type of malolactic fermentation (MLF) as well as its greenness and practicality with three other chromatographic methods such as headspace solid-phase microextraction/gas chromatography-mass spectrometry with carboxen-polydimethylosiloxane fiber (SPME/GC-MS with CAR/PDMS fiber), headspace solid-phase microextraction/gas chromatography-mass spectrometry with polyacrylate fiber (SPME/GC-MS with PA fiber), and ultra performance liquid chromatography–photodiode array detector-tandem mass spectrometry (UPLC-PDA-MS/MS). Principal Component Analysis (PCA) revealed that fingerprints obtained using all four chromatographic methods were suitable for classification using machine learning (ML). Random Forest (RF) and Support Vector Machines (SVM) yielded accuracies of at least 99% in the varietal classification of Zweigelt and Rondo wines and therefore proved suitable for robust fingerprinting-based Quality Assurance/Quality Control (QA/QC) procedures. In the case of wine classification by the type of MLF, the classifiers performed slightly worse, with the poorest accuracy of 91% for SVM and SPME/GC-MS with CAR/PDMS fiber, and no less than 93% for the other methods. Ultra-fast GC is the greenest and UPLC-PDA-MS/MS is the most practical of the four chromatographic methods. [ABSTRACT FROM AUTHOR]

Summary: The rat's model evaluated the function of sprouted beans enriched with probiotic Lactiplantibacillus plantarum 299v in alleviating dyslipidaemia, inflammation and disturbed redox homeostasis caused by a high‐lard diet. Sprouted beans improved the total antioxidant capacity of serum and liver, regardless of whether the feeds had a higher content of low‐molecular antioxidants or were additionally enriched with probiotics. The reduction of inflammation (lowered level of C‐reactive protein) and restoration of triglycerides and total cholesterol to the levels recorded in the control group (AIN‐93M) were especially observed in the group supplemented with the control adzuki bean. Introducing sprouted legumes (both the control and probiotic‐rich) improved microbiota activity affected by a high‐lard diet. The highest, desirable reduction of urease (by 80%) and tryptophanase (by 78%) activity was found in the groups fed with probiotic‐rich adzuki and mung bean sprouts respectively. Sprouted beans improve the metabolism of individuals subjected to a diet with an increased fat‐to‐carbohydrate energy ratio, especially concerning oxidative stress injury and microbiota activity. [ABSTRACT FROM AUTHOR]

The aim of this study was to investigate the qualitative and quantitative fruit profiles of ten cultivars (cvs.) of haskap berry (Lonicera caerulea var. kamtschatica Sevast.) to determine their antioxidant activity (ABTS test, CUPRAC test, ability to capture superoxide (O2˙−) and hydroxyl radicals (OH˙)), cytotoxic activity (against cancer cell lines breast, MCF-7; colon, HT-29; and melanoma, SK-Mel-28) and physicochemical properties. Most of the selected cultivars had not previously been analyzed for these properties. A total of 19 polyphenolic compounds were identified in the fruits of the tested genotypes, with a quantitative range of 2166.3–3597.0 µg/g. The polyphenol profile was dominated by anthocyanins (90.0–92.4%), and the remaining classes occurred in the following order: phenolic acids > flavonols > flavan-3-ols. The highest concentrations of these polyphenol groups were found in the cultivars 'Honeybee', 'Sinij Uties' and 'Usłada'. The fruits of these cultivars were also characterized by the highest antioxidant activity (546.6–683.5 µg/mL for O2˙− and 541.2–652.1 µg/mL for OH˙) and cytotoxic activity (103.6–649.2 µg/mL). The data obtained indicate that the fruits of the new haskap cultivars are a good source of bioactive compounds with possible health-promoting properties. [ABSTRACT FROM AUTHOR]

This research studied the phenolic content compared with the antioxidant properties of various O. vulgare (Lamiaceae) cultivars grown in Poland. The research results in this paper indicate that the dominant ingredient in all oregano cultivars was rosmarinic acid, known for its strong antioxidant properties. The highest amounts of rosmarinic acid (87.16 ± 4.03 mg/g dm) were identified in the O. vulgare spp. hirtum (Link) Ietsw. Other metabolites identified in the studied extracts include luteolin O-di-glucuronide-O-di-pentoside (30.79 ± 0.38 mg/g dm in the 'Aureum' cultivar), 4′-O-glucopyranosyl-3′, 4′-dihydroxy benzyl-protocatechuate (19.84 ± 0.60 mg/g dm in the 'Margerita' cultivar), and p-coumaroyl-triacetyl-hexoside (25.44 ± 0.18 mg/g dm in the 'Margerita' cultivar). 'Hot & spicy' and 'Margerita' cultivars were characterized by the highest activity in eliminating OH• and O2•− radicals. Extracts from Greek oregano had the highest ability to scavenge DPPH radicals and chelate iron ions. This research has also provided new evidence that oregano has anti-migratory, cytotoxic properties and influences the viability of gastric cancer cells (the highest cytotoxicity was attributed to the 'Hot & spicy' cultivar, which performed the worst in antioxidant properties tests). Extracts from the tested cultivars at a concentration of 0.625% effectively inhibited the growth of S. aureus and P. aeruginosa bacteria. It seems that the oregano grown in Poland is of good quality and can be successfully grown on a large scale if the appropriate use is found. [ABSTRACT FROM AUTHOR]

The aim of the study was to design microcapsules with a core of blueberry fruit extracts (Vaccinium myrtillus L.) using the ionotropic gelation method and then assess the effect of the type of extracts used and the combination of polymers on the profile of phenolic compounds, their in vitro bioavailability, stability during storage, as well as their antioxidant characteristics and cytotoxic activity against colon cancer cells while assessing biocompatibility against normal colon epithelial cells. Encapsulation efficiency (EE), ranging from 8.79 to 74.55%, significantly depended on the extract used and the type of carrier. It was shown that the addition of pectin (Pect) and whey protein isolate (WPI) to alginate (Alg) improved the efficiency of the encapsulation process. For this version of microcapsules, the highest antioxidant activity, phenolic compound content and their stability during storage were also demonstrated. The estimated content of phenolic compounds ranged from 0.48 to 40.07 mg/g, and the dominant compound was cyanidin 3-O-glucoside. In turn, the highest bioavailability of these compounds and the highest cytotoxic activity against cancer cells were characterized by microcapsules with Alg and WPI. Nevertheless, good biocompatibility with normal colon epithelial cells was demonstrated for all versions of microcapsules. The obtained data indicate that the tested variants of microcapsules protect the bioactive compounds of blueberry fruit extracts, which translates into maintaining their health-promoting properties. [ABSTRACT FROM AUTHOR]

Raspberry plants need intensive anti-fungal protection. A solution to this problem could be the application of an ozonation process. For this purpose, a technical solution was proposed and implemented in raspberry plant production. The proposal suggests replacing 25% of standard fungicide treatments with ozonation. It was demonstrated that the use of ozone under the proposed conditions made it possible to maintain stable parameters of chlorophyll content and fluorescence (no significant differences), but the intensity of gas exchange was increased. The greatest differences were observed in the second measurement period (T2), when the plants were in the stage of most active development. Additionally, the content and profile of low-molecular-weight antioxidants and the microbial load were determined in the collected fruits. In periods T2 and T3, the proposed method caused a reduction reaching ~2 log cfu g−1 in the microbial content of raspberry fruits. It was shown that ozone treatment intensified the biosynthesis of low-molecular-weight antioxidants in fruit (increasing the total polyphenol content by more than 20%). The proposed scheme allows a 25% reduction in standard fungicide treatments while maintaining the health of cultivated raspberry plants. The reduction in fungicide use aligns with the EU regulations and produces fruit with better quality. [ABSTRACT FROM AUTHOR]

There are several thousand different cultivars of apple trees but only a limited number are cultivated on an industrial scale. Old cultivars can be useful, for example, in the processing industry due to their different composition. In many cases, they have higher organic acid content. The content of mineral components, colour, and polyphenols in the fruit of 7 old apple tree cultivars, growing in the Wolinski National Park, was studied. The changes that occurred in the fruit after freeze-drying were also evaluated. The trees from which the fruit was picked for analysis, despite the lack of chemical protection, were fruiting and the apples had only a few symptoms of damage caused by pests or diseases. The fruit was characterised by a high soluble solids content (14.4-16.4%), in which sugars and especially fructose dominated. The number of organic acids varied greatly and ranged from 0.27 (Oberlander Himbeerapfel) g to 1.07 g/100 g (Winter Goldparmane). The content of polyphenols in fresh apples ranged from 186 mg (Horneburger Pfannkuchenapfel) to 354 mg/100 g (Winter Goldparmane) and increased after freeze-drying from 666 mg (Weisser Winterkalvill) to 1486 mg/100 g (Winter Goldparmane). The dominant group of polyphenolic compounds was phenolic acid. The freeze-drying process caused unfavourable changes in the colour of the pulp. The fruits of Oberlander Himbeerapfel cultivar were most susceptible to these changes. However, the least darkened fruit of Horneburger Pfannkuchenapfel.

Some holoparasitic species can become cultivated plants due to their unique chemical composition. A lot of bioactive contents are characteristic for them. Holoparasites of the family Orobanchaceae are known to be an important and rich source of polyphenols, especially metabolites of the phenylethanoid glycosides (PhGs) group. However, only a minority of the species in this family have been phytochemically tested. They are reported to have multiple biological and therapeutic effects and have been used for centuries in traditional Chinese medicine. This is the first study to present phytochemical profiling for a representative of genus Phelypaea. The chemical composition and biological activity in particular organs of the parasite, P. tournefortii, were determined. The interaction with its host, Tanacetum polycephalum (Asteraceae), from different places and altitudes was also studied. We presented the determination of polyphenolic compounds with the UPLC-PDA-MS/MS method, antioxidative effects and inhibitory activities, polyphenols, and nitrates content, ABTS•+, DPPH, FRAP, as well as colour parameters. The polyphenols profile of the parasite and host were different in quality and quantity. Identification of polyphenolic compounds revealed 41 compounds, 15 in the parasite (12 phenylethanoids and 3 anthocyanins), and 26 in the host (mainly flavonoids and phenolic acids). The amount and biological activity of polyphenolic compounds present in Phelypaea was very diverse and depended on the host plant and the parasite’s organs, as well as on population altitude. The results show that P. tournefortii is a potential source of functional and pro-health components. They also direct researchers’ attention to the parasite’s organs, host, and environmental influence.

The aim of the study was to evaluate the polyphenol profile of Castanea sativa leaf methanolic extract and further evaluate its biological activities in vitro. After purification with an RP-18 resin, the extract was assessed for its polyphenol profile by UPLC-PDA-MS/MS, as well as for the antioxidant potential (ABTS, CUPRAC, ChA, ROS scavenging methods), anticancer, antiobesity, antidiabetic and antimicrobial potential. Eighteen polyphenols were identified and the dominant compounds were chestatin followed by quercetin 3-O-glucoside. The total phenolic content of the extract showed a value of 1426.55 mg/100 g d.w. The obtained preparation showed the ability to scavenge O2•− (0.067 mg/mL) and OH• (0.207 mg/mL) radicals and had a stronger anti-obesity than anti-diabetic effect. Additionally, this extract exhibited a strong anticancer activity against the Caco-2 line (153.54 µg/mL), with anti-migratory and anti-proliferative activity. In turn, among the tested strains, the highest activity was demonstrated against Staphylococcus aureus. Moreover, the effects demonstrated were strongly dependent on the content of polyphenols. In conclusion, C. sativa is a promising source of natural antioxidant, antibacterial, antiobesity, antidiabetic and chemopreventive compounds for food-pharma industry; however, further experimental studies are needed to validate its pharmacological properties. [ABSTRACT FROM AUTHOR]

One of the best sources of antioxidant and health-promoting bioactive substances is the fruit of V. corymbosum. A potent oxidizing agent, ozone (O3), can effectively eliminate bacteria. The application of ozone gas to V. corymbosum fruit during storage had a favorable impact on the fruit's phenolic component and sugar content in the current investigation. After 7 days of storage, phenolic content in all highbush blueberry cultivars and clones tested increased on average by 28.60%, including anthocyanins by 34%. After 14 days of storage, an average increase of 16.50% in phenolic compounds was observed, including a 20.53% increase in anthocyanins. Among all the tested varieties, clone BOR-21 treated with a dose of 0.01 mL·L−1 ozone for 30 min after 14 days had the highest TPC—143.73 mg·100 g−1 f.w. The sugar content of berries treated with a dose of 0.01 mL·L−1 ozone for 30 min, on day 7 and day 14 of storage increased by 9.2% and 6.3%, respectively. On day 7, the highest amount of total sugar (22.74 g·100 g−1) was observed in Duke cultivar after being exposed to 0.01 mL·L−1 ozone for 15 min. The ozonation treatments enhanced the fruit's saturation with nutrients, which raises the fruit's value as food. [ABSTRACT FROM AUTHOR]

This study investigated the in vitro antioxidant and biological properties of ethanol extracts obtained from the fruits of the highbush cranberry. The produced extracts exhibited a high content of polyphenols (1041.9 mg 100 g d.m.−1) and a high antioxidant activity (2271.2 mg TE g 100 d.m.−1 using the DPPH method, 1781.5 mg TE g 100 d.m.−1 using the ABTS method), as well as a substantial amount of vitamin C (418.2 mg 100 g d.m.−1). These extracts also demonstrated significant in vitro biological activity. Studies conducted on the Saccharomyces cerevisiae cellular model revealed the strong antioxidant effects of the extract, attributed to a significant reduction in the levels of reactive oxygen species (ROS) within the cells, confirming the utility of the extracts in mitigating oxidative stress. Moreover, inhibitory properties were demonstrated against factors activating metabolic processes characteristic of inflammatory conditions. It was observed that the cranberry extract inhibits the activity of cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) non-selectively. Additionally, the extract was found to be a highly active inhibitor of acetylcholinesterase (AChE), potentially suggesting the applicability of this extract in the prevention of neurodegenerative diseases, including Alzheimer's disease. [ABSTRACT FROM AUTHOR]

BACKGROUND: Cranberries, black and red currants, strawberries, chokeberries, raspberries, blueberries and blackberries, due to the high content of polyphenolic compounds, become an excellent matrix for the production of preparations that can be used as nutraceuticals. OBJECTIVE: Evaluation of the content of bioactive compounds and health-promoting activity of preparations from polyphenolic fractions isolated from eight types of berries. METHODS: The preparations were obtained by lyophilization of the polyphenolic fraction of fruit extracts, and then they were evaluated in terms of physicochemical properties, content of polyphenolic compounds and antioxidant, antidiabetic, antiobesity and anticancer activity. RESULTS: The analyzes showed a very diverse content of bioactive compounds and a high health-promoting potential significantly depending on the type of fruit. In total, 61 polyphenolic compounds were identified, with anthocyanins being the dominant group. A positive correlation was found between the content of polyphenolic compounds and high antioxidant activity. A cytotoxic effect on colorectal adenocarcinoma cells (Caco-2) was also found. In addition, all the obtained preparations turned out to be effective inhibitors of enzymes promoting diabetes and obesity. CONCLUSIONS: The obtained preparations can be used in the production of nutraceuticals or functional food additives, applicable in the prevention and treatment of lifestyle diseases. [ABSTRACT FROM AUTHOR]

The aim of the study was to obtain a dye from black elder fruits and flowers and to study their potential in production of jellies with high antioxidant activity. Three dyes were produced by lyophilization of aqueous extracts: (1) fruits dye (F), (2) flowers dye (FL), and (3) fruits and flowers dye (F + FL). Their polyphenol profiles were compared by means of ultra-performance liquid chromatography (UPLC). The antioxidant activity [ferric reducing/antioxidant power assay (FRAP) and DPPH radicals scavenging test and total phenolics were compared by spectrophotometric methods. Jellies were produced from agar and gelatin with the addition of three obtained dyes, and their antioxidant water- and lipid soluble fractions were tested with a Photochem device. Results indicated that black elder fruits are rich in anthocyanins, especially cyanidin-3-O-sambubioside (7.56 mg/g d.w.), while flowers are rich in polyphenols, especially chlorogenic acid (2.82 mg/g d.w.) and rutin (4.04 mg/g d.w.). FL dye exhibited higher antioxidant activity compared to F dye (for about 30−40%), regardless of the used method, whereas F + FL dye was characterized by intermediate antioxidant activity. Jellies produced with the addition of FL dye had better antioxidant properties but unattractive color and unpleasant taste, but the use of F + FL dye created a product of favorable organoleptic properties and antioxidant activity comparable to jellies with F dye addition.