1. Relationship between Antibody Levels, IgG Binding toPlasmodium falciparum-Infected Erythrocytes, and Disease Outcome in Hospitalized Urban Malaria Patients from Dakar, Sénégal
- Author
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Marie Louise Varela, Rokhaya Ndiaye Diallo, Alioune Dieye, Birahim Niang, Moustapha Mbow, Antoine Marie Diatta, Maguette Sylla Niang, Ronald Perraut, Mouhamadou Mansour Fall, Kantome Ndiaye, and Babacar Mbengue
- Subjects
Male ,0301 basic medicine ,Erythrocytes ,Article Subject ,Plasmodium falciparum ,Malaria, Cerebral ,lcsh:Medicine ,Antibodies, Protozoan ,Parasitemia ,General Biochemistry, Genetics and Molecular Biology ,Flow cytometry ,03 medical and health sciences ,Antigen ,parasitic diseases ,medicine ,Humans ,Parasite hosting ,Malaria, Falciparum ,General Immunology and Microbiology ,biology ,medicine.diagnostic_test ,business.industry ,lcsh:R ,General Medicine ,medicine.disease ,biology.organism_classification ,Virology ,Senegal ,030104 developmental biology ,IgG binding ,Cerebral Malaria ,Immunoglobulin G ,Immunology ,Female ,business ,Malaria ,Research Article - Abstract
Background. Management of clinical malaria requires the development of reliable diagnostic methods and efficient biomarkers for follow-up of patients. Protection is partly based on IgG responses to parasite antigens exposed at the surface of infected erythrocytes (iRBCs). These IgG responses appeared low during clinical infection, particularly in severe disease.Methods. We analyzed the IgG binding capacity to the surface of live erythrocytes infected by knob positive FCR3 strain. Sera from 69 cerebral malaria (CM) and 72 mild malaria (MM) cases were analyzed by ELISA for IgG responses to five antigens from iRBC and by flow cytometry for IgG binding as expressed in labeling index ratio (LIR). The relationship between IgG levels, LIR, parasitemia, age, and the clinical outcomes was evaluated.Results. We found a significant decrease of LIR in adult CM fatal cases compared to surviving patients (p=0.019). In MM, LIRs were correlated to IgG anti-iRBC and anti-PfEMP3/5 levels. In CM, no correlation was found between LIR, IgG levels, and parasitemia.Conclusion. The IgG binding assay was able to discriminate outcome of cerebral malaria cases and it deserves further development as a potential functional-associated assay for symptomatic malaria analysis.
- Published
- 2016
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