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Indoor lighting, incorporated with visual and circadian needs, is the upcoming goal of lighting designers. In tropical and subtropical regions, where more daylight is available, daylight harvesting is a prime source of ambient lighting indoors. This paper aims to study various cases of lighting ambience to investigate the circadian lighting capability in terms of the circadian stimulus (CS) of the system under consideration. The instances considered are simulation studies in an open office plan and real-time experimentations in a test workbench and a faculty cabin. Daylight integration was undertaken through controlled venetian blinds, tunable sources and a commercially available human-centric lighting system. The result and analysis show the influence of spectrally tunable light sources on CS rather than fixed light sources. Due to the varying CCT of tunable LED luminaire, circadian stimulation for an occupant can be easily incorporated without crossing the limits of vertical and horizontal illuminance, which may lead to visual discomfort. The findings from this study reveal that daylight–artificial light integration scheme with controlled shading and spectrally tunable source provides the optimal solution for glare-free, energy-effective and circadian entrainment, i.e. human-centric lighting (HCL). With the help of simulations, pre-evaluation will aid the lighting engineers in making a better choice among the various lighting-controlled schemes to implement HCL in indoor office spaces.