Search

Microdochium sichuanense L.S. Dissan., J.C. Kang & Maharachch., sp. nov. (FIGUER 2) Index Fungorum Number: IF900348, Facesoffungi: 14277 Etymology:— Referring to the location “ Sichuan Province ” where the fungus was collected Holotype:—KUN-HKAS127240 Saprobic on dead leaves of Poaceae in terrestrial habitats. Sexual morph: Ascomata 100–180 μm high, 60–100 μm diam. (x̅ = 146.3 × 86.9 μm, n = 5), solitary, scattered, appear as black dots on the host surface, square to ellipsoidal, brown, immersed, apapillate, ostiolate. Peridium 10–20 μm wide, evenly thickened, comprising brown cells of textura angularis to textura prismatica, flattened and hyaline towards inner layers, fused with host tissues. Hamathecium comprising hyaline, cylindrical, guttulate, branched paraphyses (2–6 μm wide), arising from base of ascomata. Asci 90–140 × 15–20 μm (x̅ = 110 × 18 μm, n = 15), unitunicate, 8-spored, overlapping 1–2 seriate, clavate to oblong, with a minute J- apical ring or without an epical ring, with a short stipe. Ascospores 10–22 × 4–7 μm (x̅ = 16 × 5.5 μm, n = 15), hyaline, oval to fusiform, sometimes allantoid, straight or slightly curved, guttulate. Asexual morph: Undetermined Culture characteristics:— Colonies on PDA reaching 2 cm diam. after 2 weeks at 20–25 °C, medium dense, circular to slightly irregular, slightly raised, cottony surface smooth, at first white, becoming light yellow when mature, without any pigmentation; reverse yellowish-white. Material examined:— China, Sichuan Province, Chengdu, University of Electronic Science and Technology premises, on dead leaves of an unidentified poaceous host, September 2021, Q Wang W30 (KUN-HKAS127240, holotype), ex-type cultures KUNCC 23–13008 Known distribution:— Sichuan Province, China Notes:— Microdochium sichuanense morphologically resembles M. graminearum and M. shilinense in having solitary, scattered, immersed, apapillate, ostiolate ascomata, textura angularis to prismatica cells in peridium, 8- spored, biseriate with short stipe asci, and hyaline, guttulate, fusiform, straight or curved ascospores. Microdochium sichuanense differs from M. graminearum and M. shilinense in having clavate to oblong asci without an apical ring and aseptate ascospores, while M. graminearum and M. shilinense have fusiform asci with apical ring and 0–3 transversely septate ascospores. Multi-gene phylogenetic analyses (FIGURE 1) show that our collection forms a sister group with the isolates of M. hainanense (SAUCC 210781 and SAUCC 210782) with 60% ML and 0.96 BYPP. It is impossible to compare M. sichuanense and M.hainanense as they occur in different morphs (sexual and asexual). A pairwise nucleotide comparison between the two species showed that M. sichuanense differs from M. hainanense (SAUCC 210782) in 35/566 bp of ITS (6.18%) and 150/797 bp of rpb2 (18.82%). Interestingly, both species were collected from the same host family (Poaceae), but from different locations in China (M. sichuanense: Sichuan Province and M. hainanense: Hainan Province)., Published as part of Dissanayake, Lakmali S., Kang, Ji-Chuan & Maharachchikumbura, Sajeewa S. N., 2023, Microdochium sichuanense sp. nov. (Microdochiaceae, Xylariales), from a Poaceae host in Sichuan, China, pp. 206-216 in Phytotaxa 600 (3) on pages 211-212, DOI: 10.11646/phytotaxa.600.3.7, http://zenodo.org/record/8080903

Dissanayake, Lakmali S., Kang, Ji-Chuan, Maharachchikumbura, Sajeewa S.N. (2023): Microdochium sichuanense sp. nov. (Microdochiaceae, Xylariales), from a Poaceae host in Sichuan, China. Phytotaxa 600 (3): 206-216, DOI: 10.11646/phytotaxa.600.3.7, URL: http://dx.doi.org/10.11646/phytotaxa.600.3.7

Neostagonosporella bambusicola L.S. Dissan. & J.C. Kang sp. nov. (FIGURE 2) Index Fungorum number: IF559874; Facesoffungi number: FoF09712 Etymology— Referring to the host “bamboo” from which the holotype was isolated. Holotype — HKAS115852 Saprobic on dead leaves of bamboo (Poaceae). Sexual morph: Ascostromata 180–220 × 140–180 μm (x = 201 × 163 μm, n = 10), ellipsoidal, globose to subglobose or irregular in shape, semi-immersed in host epidermis, coriaceous, solitary to gregarious, multiloculate, erumpent through host tissue, with dark brown to black, glabrous, with a centrally located ostiole. Peridium 12–20 μm wide (x = 15 μm, n = 10), thin at base, thick at sides towards apex, upper part fused with host tissue, composed of two layers; outer layer: pale to brown pseudoparenchymatous cells of textura angularis, inner layer: hyaline, somewhat flattened cells of textura angularis. Hamathecium composed of 1–3 μm (x = 2 μm, n = 10) wide, cellular, septate pseudoparaphyses, embedded in a hyaline gelatinous matrix. Asci 70–130 × 13.5–15 μm (x = 92 × 13 μm, n = 20), 8-spored, bitunicate, fissitunicate, cylindrical to cylindric-clavate, short pedicellate (up to 10 μm), apically rounded with an ocular chamber. Ascospores 30–36 × 5.5–7.2 μm (x = 33 × 6 μm, n = 20), partially overlapping uni- to bi-seriate, hyaline, cylindrical to fusiform or cylindric-clavate, with acute ends, narrower towards end cells, straight or slightly curved, 5-7-septate, slightly constricted at septa, nearly equidistant between septa, guttulate, smooth-walled, surrounded by a 10–14 μm (x = 11 μm, n = 10) thick mucilaginous sheath. Asexual morph: Undetermined. Culture characteristics— Colonies on PDA, reaching 2 cm diam., after 2 weeks at 20°C, medium dense, circular to slightly irregular with uneven margin, slightly raised and cottony surface, colony from above: white at the margin, pale-grey at the center. From below: yellowish margin, yellow to brown at the center; mycelium pale-grey. Material examined— CHINA, Guizhou Province, Guiyang, Guizhou University, on dead leaves of bamboo, 28 May 2019, L. S. Dissanayake (HKAS115852, holotype), ex-type culture (KUMCC 20-0031). Known distribution — Guizhou Province, China Notes: Neostagonosporella bambusicola shares similar morphology with N. sichuanensis showing multiloculate ascostromata and cylindrical to fusiform, transversely multi-septate, straight or curved ascospores, which are widest at the central cells. In the present phylogenetic analyses, N. bambusicola clusters sister to N. sichuanensis with relatively high statistical support (Figure 1). The two species are easily distinguishable since Neostagonosporella bambusicola has smaller ascomata (180–220 × 140–180 μm), cellular, septate pseudoparaphyses and ascospores with thick sheath (10–14 μm), whereas N. sichuanensis has large ascomata (1–2 mm × 230–340 μm), trabeculate pseudoparaphyses and ascospores with a thin sheath (5–9 μm)., Published as part of Dissanayake, Lakmali S., Marasinghe, Diana S., Thambugala, Kasun M. & Kang, Ji-Chuan, 2022, Neostagonosporella bambusicola sp. nov. (Phaeosphaeriaceae, Pleosporales) from bamboo in China, pp. 262-274 in Phytotaxa 573 (2) on pages 265-267, DOI: 10.11646/phytotaxa.573.2.6, http://zenodo.org/record/7349978

Xylaria wuzhishanensis Y.P. Wu & Q.R. Li, sp. nov. (Fig. 5) MycoBank number: MB 842170 Type:— CHINA, Hainan Province, Wuzhishan City, Wuzhishan Nature Reserve (18.541805°N, 109.412245°E), elev. 892 m, on dead wood of a plant roots (Oleaceae sp.), November 2020, Youpeng Wu and Lili Liu, 2020WZS35 (GMB0074, holotype; KUN-HKAS 12634, isotype; ex-type living culture, GMBC0074). Etymology: Referring to its collection location, Wuzhishan Mountain from where the species was first isolated (China). Saprobic on dead wood roots, forming on the host surface. Sexual morph: Stromata 0.3–0.8 cm long × 0.6–1.2 cm broad (x = 0.6 × 0.8 cm, n = 10), semiglobular or irregularly globose, whole stromata up to with flattened or slightly convex top. Surface of stromata black, with raised blackish dots. Perithecia 300–600 μm high × 200–400 μm diam. (x = 400 × 300 μm, n = 10), oval to spherical, embedded, closely arranged, interior white. Ostioles papillate, surrounded with white tissue. Asci 235–366 × 11–20 μm (x = 293 × 14.2 μm, n = 30), 8-spored, unitunicate, cylindrical, long-stipitate, apically rounded, with a J+, rectangular or urn-shaped, apical ring, blue staining in Melzer’s reagent, 6.7–10.5 μm (x = 8.7 μm, n = 30) high, 4.2–6.5 μm (x = 5.3 μm, n = 30) wide. Ascospores 23–30.5 × 7–9 μm (x = 26.5 × 8 μm, n=30), brown to black, unicellular, ellipsoid to inequilateral, with broadly rounded ends, smooth, with a conspicuous slanted germ slit nearly one-third spore-length, lacking appendages and sheaths. Asexual morph: Undetermined. Culture characteristics: Colonies on OA reaching 3–4 mm diam. after 2 weeks at 25 °C. White at first, white at first, upright, velvety with irregular outgrowths or furrows, with a peripheral fan-shaped extension spreading toward the edge; reverse light grey in the center, white marginal area. After 4 weeks, colonies on OA reaching 9 cm diam., white greyish, velvety or inflorescence, appressed with entire margins; reverse black, with brownish ring. No conidia were observed. Notes: Based on the multi-gene phylogenetic analyses (Fig. 1), X. wuzhishanensis is most closely related to X. schweinitzii. Morphologically, X. wuzhishanensis differs from X. schweinitzii by its semi-globular or irregularly globose stromata. Xylaria wuzhishanensis shows similar traits of X. atrosphaerica (Cooke & Massee) Callan & J.D. Rogers, X. globosa (Spreng. ex Fr.: Fr.) Mont., and X. lepidota Y.M. Ju et al. with semiglobular or irregularly globose stromata. But, X. wuzhishanensis can distinguish from X. atrosphaerica by its larger ascospores (23–30.5 × 7–9 μm vs. 17–25 × 6–10 μm) with a shorter germ slit (Callan et al. 1990). The surface of stroma lacks crackle of X. wuzhishanensis, which can make a distinction between X. globosa and X. lepidota (Ju et al. 2009, Fournier et al. 2018)., Published as part of Wu, You-Peng, Pi, Yin-Hui, Long, Si-Han, Liu, Li-Li, Zhang, Xu, Long, Qing-De, Lin, Yan, Kang, Ying-Qian, Kang, Ji-Chuan, Wijayawardene, Nalin N. & Li, Qi-Rui, 2022, Morphology and phylogeny reveal two novel Xylaria (Xylariaceae) species from China, pp. 130-146 in Phytotaxa 550 (2) on page 141, DOI: 10.11646/phytotaxa.550.2.3, http://zenodo.org/record/6641065, {"references":["Callan, B. E. & Rogers, J. D. (1990) Teleomorph-anamorph connections and correlations in some Xylaria species. Mycotaxon 36: 343 - 369.","Ju, Y. M., Hsieh, H. M., Vasilyeva, L. & Akulov, A. (2009) Three new Xylaria species from Russian Far East. Mycologia 101: 548 - 553. http: // doi. org / 10.3852 / 08 - 188","Fournier, J., Lechat, C. & Courtecuisse, R. (2018) The genus Xylaria sensu lato (Xylariaceae) in Guadeloupe and Martinique (French West Indies) I. Taxa with penzigioid stromata. Ascomycete. org 10 (4): 131 - 176. http: // doi. org / 10.25664 / ART- 0239"]}