Search

Objective To understand the HIV infection risk perception bias and its related influencing factors among young men who have sex with men (MSM) in Shandong Province, so as to provide reference for making feasible HIV health education plans. Methods From April to July, 2022, young MSM were recruited in six cities in Shandong province, and a questionnaire survey was conducted and blood samples were collected to collect demographic characteristics, HIV risk perception and risk behavior of MSM. Logistic regression model was used to analyze the influencing factors of HIV infection risk perception bias among young MSM. Results A total of 978 MSM were investigated, of which 85%(831/978) were over 20 years old, 97.6%(955/978) were unmarried/divorced or widowed, 61.1%(598/978) were college educated, 2.6% (25/978) were positive for HIV antibody and 3.6%(35/978) for syphilis antibody. 47.1%(461/978) had HIV infection risk perception bias, of which 54.0%(249/461) underestimate the risk and 46.0%(212/461) overestimate the risk. Logistic regression analysis showed that unmarried/divorced/widowed (OR = 11.693, 95%CI: 1.546-88.407) and having no or low risk of new drugs (OR = 2.041, 95%CI: 1.477-2.825) were the risk factors for underestimating the risk. Household registration in Shandong province (OR = 2.618, 95%CI: 1.397-4.902), college education or above (OR = 1.728, 95%CI: 1.206-2.475), homosexuality (OR = 2.532, 95%CI: 1.093-5.845), having general or high risk of new drugs(OR = 8.323, 95%CI: 4.602-15.052), who had not received AIDS intervention (OR = 2.288, 95%CI: 1.224-4.292) and had not taken preventive drugs before exposure (OR = 11.905, 95%CI: 3.663-38.462) were the risk factors for overestimating the risk. Conclusion HIV infection risk perception bias among young MSM in Shandong Province is high, and HIV risk behaviors are widespread. It is necessary to strengthen the education of MSM who have low self-rated HIV infection risk but have high-risk behaviors, improve risk awareness and reduce the occurrence of dangerous behaviors. [ABSTRACT FROM AUTHOR]

Abstract Background Information on the health-related quality of life (HRQoL) of patients with genital warts (GW) in populations in mainland China is still limited. The aim of the study was to use a generic instrument to measure the impact of genital warts on HRQoL in men and women in this setting. Methods A multi-centre hospital-based cross-sectional study across 18 centers in China was conducted to interview patients using the European quality of life-5 dimension (EQ-5D) instrument; respondents' demographic and clinical data were also collected. Results A total of 1,358 GW patients (612 men, 746 women) were included in the analysis, with a mean age of 32.0 ± 10.6 years. 56.4% of the patients reported some problems in the dimension of Anxiety/Depression (highest), followed by Pain/Discomfort (24.7%) and Mobility (3.5%). The overall visual analogue scale (VAS) score of the study population was found to be 65.2 ± 22.0, and the EQ-5D index score was found to be 0.843 ± 0.129 using Japanese preference weights (the Chinese preference was unavailable yet). Patients with lower VAS means and EQ-5D index scores were more often female, living in urban area, and suffering multiple GW (all p values < 0.05), but the values did not differ notably by age (p values > 0.05). Conclusions The HRQoL of patients with GW was substantially lower, compared to a national representative general population in China (VAS = ~80); the findings of different subgroups are informative for future GW prevention and control efforts.

Schisandra lignans, mainly including schizandrol A, schizandrol B, schisantherin A, schizandrin A, schizandrin B, etc., are the major active ingredients of Schisandra chinensis. In the present study, a robust liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed for the simultaneous quantification of schisandra lignans in rat primary hepatocytes. Lovastatin was used as an internal standard, and chromatographic separation was achieved on a Shimadzu C18 column with a gradient elution at the flow rate of 0.2 mL/min. All of the analytes were detected in multiple reaction monitoring mode with positive electrospray ionization since the sodium adduct ion [M + Na]+ was observed as the most intensive peak in the MS spectrum. For schizandrol A, schisantherin A and schizandrin A, the dynamic range was within 2-1000 ng/mg protein, and the linear range of schizandrol B and schizandrin B was from 5 to 1000 ng/mg protein. The intra- and inter-day precision was <15% and the accuracy (relative error) ranged from −15 to 15%. No significant variation was observed in the stability tests. The validated method was then successfully applied to the time-dependent uptake study for the Schisandra Lignan Extract in rat primary hepatocytes. [ABSTRACT FROM AUTHOR]

Abstract: Objective: To take effective strategies and measures for the prevention and control of hemorrhagic fever with renal syndrome (HFRS) endemic areas by investigating its dynamic geographical boundaries in Shandong Province, China. Methods: The incidence of HFRS from 1982 to 2008 in Shandong Prvince, China, was detected with inverse distance weighting (IDW) interpolation based on geographical information system (GIS). Dynamic geographical boundaries of HFRS endemic areas in Shandong Province, China, were analyzed by geographical boundary analysis. Results: The HTN-type endemic areas of HFRS were located in Linyi City in phase 1 (1982–1986), the SEO-type endemic areas of HFRS were located in Jining City in phase 2 (1987–2003), and the endemic areas of HFRS in Jining City gradually disappeared and the endemic areas of HFRS with mixed-types of reservoir rodents were located in Linyi City in phase 3 (2004–2008). Meanwhile, new endemic areas emerged in the northwestern Shandong province, China. Conclusion: The SEO-type endemic areas of HFRS are located in western Shandong Province, China, and the HTN-type endemic areas of HFRS are located eastern Shandong Province, Chin, indicating that the endemic areas of HFRS should be vaccinated and rodents should be controlled. [Copyright &y& Elsevier]

The synergistic effect and compatibility structure of active anti-inflammatory ingredients(iridoid glycosides: shanzhiside methylester and 8-O-acetylshanzhiside methyl ester, flavonoid glycoside: luteoloside, and phenylethanoid glycoside: forsythoside B) from Lamiophlomis rotata were explored based on network pharmacology and component structure theory. In network pharmacology, CTD, SwisseTargetPrediction, and PharmMapper databases were used to collect and screen the targets of all active ingredients. The inflammation-related targets were obtained from CTD and GeneCards databases. The core targets were obtained by Venny 2.1.0, STRING, and Cytoscape 3.9.1. Core targets were annotated by the GO function and enriched by the KEGG pathway based on the DAVID database. In terms of component structure, based on a uniform design method and xylene-induced ear swelling model in mice, tumor necrosis factor-α and interleukin-6 were taken as the dependent variables, and the compatibility relationship among anti-inflammatory ingredients from L. rotata was explored through the quadratic polynomial stepwise regression. In addition, in vivo pharmacological experiments were conducted to verify the results. A network pharmacology study showed that compared with a single ingredient, the combined action of the three ingredients can synergistically exert anti-inflammatory effects through more biological processes, pathways, and targets. Component structure study showed that the optimal structural ratio of shanzhiside methylester and 8-O-acetylshanzhiside methyl ester in the iridoid glycoside ingredient was 1.21∶1. The optimal structural ratio among the three types of ingredients(iridoid glycosides∶phenylethanol glycoside∶flavonoid glycoside) was 4.8∶1.6∶1. In conclusion, each anti-inflammatory ingredient from L. rotata can work synergistically, and there is an optimal compatibility ratio relationship among these ingredients. This work provides a new experimental basis for the intrinsic quality control of L. rotata.