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207 results on '"Kanamaru, Kengo"'

15. Arabidopsis EGY1 Is Critical for Chloroplast Development in Leaf Epidermal Guard Cells

Author

Sanjaya, Alvin, primary, Muramatsu, Ryohsuke, additional, Sato, Shiho, additional, Suzuki, Mao, additional, Sasaki, Shun, additional, Ishikawa, Hiroki, additional, Fujii, Yuki, additional, Asano, Makoto, additional, Itoh, Ryuuichi D., additional, Kanamaru, Kengo, additional, Ohbu, Sumie, additional, Abe, Tomoko, additional, Kazama, Yusuke, additional, and Fujiwara, Makoto T., additional

Published
2021
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16. An Argon-Ion-Induced Pale Green Mutant of Arabidopsis Exhibiting Rapid Disassembly of Mesophyll Chloroplast Grana

Author

Sanjaya, Alvin, Kazama, Yusuke, Ishii, Kotaro, Muramatsu, Ryohsuke, Kanamaru, Kengo, Ohbu, Sumie, Abe, Tomoko, T Fujiwara, Makoto, Kotaro, Ishii, Sanjaya, Alvin, Kazama, Yusuke, Ishii, Kotaro, Muramatsu, Ryohsuke, Kanamaru, Kengo, Ohbu, Sumie, Abe, Tomoko, T Fujiwara, Makoto, and Kotaro, Ishii

Abstract

Argon-ion beam is an effective mutagen capable of inducing a variety of mutation types. In this study, an argon ion-induced pale green mutant of was isolated and characterized. The mutant, designated Ar50-33-pg1, exhibited moderate defects of growth and greening and exhibited rapid chlorosis in photosynthetic tissues. Fluorescence microscopy confirmed that mesophyll chloroplasts underwent substantial shrinkage during the chlorotic process. Genetic and whole-genome resequencing analyses revealed that Ar50-33-pg1 contained a large 940 kb deletion in chromosome V that encompassed more than 100 annotated genes, including 41 protein-coding genes such as /, , and . One of the deleted genes, , for a thylakoid membrane-localized metalloprotease, was the major contributory gene responsible for the pale mutant phenotype. Both an mutant and F progeny of an Ar50-33-pg1 × cross-exhibited chlorotic phenotypes similar to those of Ar50-33-pg1. Furthermore, ultrastructural analysis of mesophyll cells revealed that Ar50-33-pg1 and initially developed wild type-like chloroplasts, but these were rapidly disassembled, resulting in thylakoid disorganization and fragmentation, as well as plastoglobule accumulation, as terminal phenotypes. Together, these data support the utility of heavy-ion mutagenesis for plant genetic analysis and highlight the importance of in the structural maintenance of grana in mesophyll chloroplasts.

Published
2021

23. MOESM1 of 5-Aminolevulinic acid fermentation using engineered Saccharomyces cerevisiae

Author

Hara, Kiyotaka, Saito, Masaru, Kato, Hiroko, Morikawa, Kana, Kikukawa, Hiroshi, Nomura, Hironari, Fujimoto, Takanori, Hirono-Hara, Yoko, Watanabe, Shigeyuki, Kanamaru, Kengo, and Kondo, Akihiko

Abstract

Additional file 1. Table S1. Effect of glycine addition on intracellular metabolites in HEM1-overexpressing S. cerevisiae. Figure S1. Relative mRNA levels in HEM1-, HEM1/ACO1- and HEM1/ACO2-overexpressing strains.

Published
2019
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24. A novel member of the cspA family of genes that is induced by cold shock in Escherichia coli

Author

Nakashima, Kyoko, Kanamaru, Kengo, Mizuno, Takeshi, and Horikoshi, Koki

Subjects
Escherichia coli -- Research, Amino acid sequence -- Observations, Bacterial proteins -- Analysis, Biological sciences
Abstract

The cspG gene is a low-temperature-inducible gene found at 22 min in Escherichia coli chromosomes. The gene is a member of the cspA family of genes encoding cold shock proteins. It encodes a protein consisting of 70 amino acids that is 73% identical to CspA and 77% identical to CspB E. coli proteins. The gene sequence of cspG is significantly similar to that of cspA and cspB in the coding regions and the 5'-upstream noncoding regions.

Published
1996

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