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2. In-air microfluidics enables rapid fabrication of emulsions, suspensions, and 3D modular (bio)materials

Author

Visser, Claas Willem, Kamperman, Tom, Karbaat, Lisanne P., Lohse, Detlef, and Karperien, Marcel

Subjects
Physics - Fluid Dynamics, Physics - Applied Physics
Abstract

Microfluidic chips provide unparalleled control over droplets and jets, which have advanced all natural sciences. However, microfluidic applications could be vastly expanded by increasing the per-channel throughput and directly exploiting the output of chips for rapid additive manufacturing. We unlock these features with in-air microfluidics, a new chip-free platform to manipulate microscale liquid streams in the air. By controlling the composition and in-air impact of liquid microjets by surface tension-driven encapsulation, we fabricate monodisperse emulsions, particles, and fibers with diameters of 20 to 300um at rates that are 10 to 100 times higher than chip-based droplet microfluidics. Furthermore, in-air microfluidics uniquely enables module-based production of three-dimensional (3D) multiscale (bio)materials in one step because droplets are partially solidified in-flight and can immediately be printed onto a substrate. In-air microfluidics is cytocompatible, as demonstrated by additive manufacturing of 3D modular constructs with tailored micro-environments for multiple cell types. Its in-line control, high throughput and resolution, and cytocompatibility make in-air microfluidics a versatile platform technology for science, industry, and health care.

Published
2020
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9. Tunable and Compartmentalized Multimaterial Bioprinting for Complex Living Tissue Constructs

Author

Hassan, Shabir, primary, Gomez-Reyes, Eduardo, additional, Enciso-Martinez, Eduardo, additional, Shi, Kun, additional, Campos, Jorge Gonzalez, additional, Soria, Oscar Yael Perez, additional, Luna-Cerón, Eder, additional, Lee, Myung Chul, additional, Garcia-Reyes, Isaac, additional, Steakelum, Joshua, additional, Jeelani, Haziq, additional, García-Rivera, Luis Enrique, additional, Cho, Minsung, additional, Cortes, Stephanie Sanchez, additional, Kamperman, Tom, additional, Wang, Haihang, additional, Leijten, Jeroen, additional, Fiondella, Lance, additional, and Shin, Su Ryon, additional

Published
2022
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10. Embedded 3D Printing in Self‐Healing Annealable Composites for Precise Patterning of Functionally Mature Human Neural Constructs (Adv. Sci. 25/2022)

Author

Kajtez, Janko, primary, Wesseler, Milan Finn, additional, Birtele, Marcella, additional, Khorasgani, Farinaz Riyahi, additional, Rylander Ottosson, Daniella, additional, Heiskanen, Arto, additional, Kamperman, Tom, additional, Leijten, Jeroen, additional, Martínez‐Serrano, Alberto, additional, Larsen, Niels B., additional, Angelini, Thomas E., additional, Parmar, Malin, additional, Lind, Johan U., additional, and Emnéus, Jenny, additional

Published
2022
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13. Embedded 3D Printing in Self-Healing Annealable Composites for Precise Patterning of Functionally Mature Human Neural Constructs

Author

Kajtez, Janko, Wesseler, Milan Finn, Birtele, Marcella, Khorasgani, Farinaz Riyahi, Rylander Ottosson, Daniella, Heiskanen, Arto, Kamperman, Tom, Leijten, Jeroen, Martínez-Serrano, Alberto, Larsen, Niels B., Angelini, Thomas E., Parmar, Malin, Lind, Johan U., Emnéus, Jenny, Kajtez, Janko, Wesseler, Milan Finn, Birtele, Marcella, Khorasgani, Farinaz Riyahi, Rylander Ottosson, Daniella, Heiskanen, Arto, Kamperman, Tom, Leijten, Jeroen, Martínez-Serrano, Alberto, Larsen, Niels B., Angelini, Thomas E., Parmar, Malin, Lind, Johan U., and Emnéus, Jenny

Abstract

Human in vitro models of neural tissue with tunable microenvironment and defined spatial arrangement are needed to facilitate studies of brain development and disease. Towards this end, embedded printing inside granular gels holds great promise as it allows precise patterning of extremely soft tissue constructs. However, granular printing support formulations are restricted to only a handful of materials. Therefore, there has been a need for novel materials that take advantage of versatile biomimicry of bulk hydrogels while providing high-fidelity support for embedded printing akin to granular gels. To address this need, Authors present a modular platform for bioengineering of neuronal networks via direct embedded 3D printing of human stem cells inside Self-Healing Annealable Particle-Extracellular matrix (SHAPE) composites. SHAPE composites consist of soft microgels immersed in viscous extracellular-matrix solution to enable precise and programmable patterning of human stem cells and consequent generation mature subtype-specific neurons that extend projections into the volume of the annealed support. The developed approach further allows multi-ink deposition, live spatial and temporal monitoring of oxygen levels, as well as creation of vascular-like channels. Due to its modularity and versatility, SHAPE biomanufacturing toolbox has potential to be used in applications beyond functional modeling of mechanically sensitive neural constructs.

Published
2022

14. Embedded 3D printing in self-healing annealable composites for functional patterning of human neural constructs

Author

Kajtez, Janko, Wessler, Milan Finn, Birtele, Marcella, Khorasgani, Farinaz Riyahi, Rylander Ottosson, Daniella, Heiskanen, Arto, Kamperman, Tom, Leijten, Jeroen, Martínez-Serrano, Alberto, Larsen, Niels B., Angelini, Thomas E., Parmar, Malin, Lind, Johan U., Emnéus, Jenny, TechMed Centre, and Developmental BioEngineering

Abstract

Human in vitro models of neural tissue with controllable cellular composition, tunable microenvironment, and defined spatial patterning are needed to facilitate studies of brain development and disease. Towards this end, bioprinting has emerged as a promising strategy. However, precise and programmable printing of extremely soft and compliant materials that are permissive for stem cell differentiation and functional neuronal growth has been a major challenge. Therefore, solutions for engineering structurally and functionally defined human neural constructs remain scarce. Here, we present a modular platform for bioengineering of neuronal networks via direct embedded 3D printing of human stem cells inside Self-Healing Annealable Particle-Extracellular matrix (SHAPE) composites. The approach combines rheological benefits of granular soft microgel supports with the versatile biomimicry of bulk hydrogels to simultaneously enable precise freeform patterning of stem cells, and consequent generation and long-term maintenance of subtype-specific neurons within engineered networks that extend into the bulk of the annealed support. The developed approach further allows multi-ink deposition, live spatial and temporal monitoring of oxygen levels, as well as creation of vascular channels. Due to its modularity, SHAPE biomanufacturing toolbox not only offers a solution for functional modeling of mechanically sensitive neural constructs, but also has potential to be applied to a wide range of biomaterials with different crosslinking mechanisms to model tissues and diseases where recapitulation of complex architectural features and topological cues is essential.

Published
2021

15. Tethering Cells via Enzymatic Oxidative Crosslinking Enables Mechanotransduction in Non‐Cell‐Adhesive Materials (Adv. Mater. 42/2021)

Author

Kamperman, Tom, primary, Henke, Sieger, additional, Crispim, João F., additional, Willemen, Niels G. A., additional, Dijkstra, Pieter J., additional, Lee, Wooje, additional, Offerhaus, Herman L., additional, Neubauer, Martin, additional, Smink, Alexandra M., additional, Vos, Paul, additional, Haan, Bart J., additional, Karperien, Marcel, additional, Shin, Su Ryon, additional, and Leijten, Jeroen, additional

Published
2021
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16. Embedded 3D printing in self-healing annealable composites for precise patterning of functionally mature human neural constructs

Author

Kajtez, Janko, primary, Wesseler, Milan Finn, additional, Birtele, Marcella, additional, Khorasgani, Farinaz Riyahi, additional, Ottosson, Daniella Rylander, additional, Heiskanen, Arto, additional, Kamperman, Tom, additional, Leijten, Jeroen, additional, Martínez-Serrano, Alberto, additional, Larsen, Niels B., additional, Angelini, Thomas E., additional, Parmar, Malin, additional, Lind, Johan U., additional, and Emnéus, Jenny, additional

Published
2021
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17. hiPSC-derived 3D Bioprinted Skeletal Muscle Tissue Implants Regenerate Skeletal Muscle Following Volumetric Muscle Loss

Author

Jodat, Yasamin A., Zhang, Ting, Tanoury, Ziad Al, Kamperman, Tom, Shi, Kun, Huang, Yike, Panayi, Adriana, Endo, Yori, Wang, Xichi, Quint, Jacob, Arnaout, Adnan, Kiaee, Kiavash, Hassan, Shabir, Lee, Junmin, Martinez, Angel Flores Huidobro, Ochoa, Sofia Lara, Lee, KangJu, Calabrese, Michelle, Carlucci, Alessandro, Tamayol, Ali, Sinha, Indranil, Pourquié, Olivier, Ryon Shin, Su, Developmental BioEngineering, and TechMed Centre

Abstract

Engineering of biomimetic tissue implants provides an opportunity for repairing volumetric muscle loss (VML), beyond a tissue’s innate repair capacity. Here, we present thick, suturable, and pre-vascularized 3D muscle implants containing human induced pluripotent stem cell-derived myogenic precursor cells (hiPSC-MPCs), which can differentiate into skeletal muscle cells while maintaining a self-renewing pool. The formation of contractile myotubes and millimeter-long fibers from hiPSC-MPCs is achieved in chemically, mechanically, and structurally tailored extracellular matrix-based hydrogels, which can serve as scaffolds to ultimately organize the linear fusion of myoblasts. Embedded multi-material bioprinting is used to deposit complex patterns of perfusable vasculatures and aligned hiPSC-MPC channels within an endomysium-like supporting gel to recapitulate muscle architectural integrity in a facile yet highly rapid manner. Moreover, we demonstrate successful graft-host integration and de novo muscle formation upon in vivo implantation of pre-vascularized constructs within a VML model. This work pioneers the engineering of large pre-vascularized hiPSC-derived muscle tissues toward next generation VML regenerative therapies.

Published
2021

18. hiPSC-derived 3D Bioprinted Skeletal Muscle Tissue Implants Regenerate Skeletal Muscle Following Volumetric Muscle Loss

Author

Jodat, Yasamin A., primary, Zhang, Ting, additional, Tanoury, Ziad Al, additional, Kamperman, Tom, additional, Shi, Kun, additional, Huang, Yike, additional, Panayi, Adriana, additional, Endo, Yori, additional, Wang, Xichi, additional, Quint, Jacob, additional, Arnaout, Adnan, additional, Kiaee, Kiavash, additional, Hassan, Shabir, additional, Lee, Junmin, additional, Martinez, Angel Flores Huidobro, additional, Ochoa, Sofia Lara, additional, Lee, KangJu, additional, Calabrese, Michelle, additional, Carlucci, Alessandro, additional, Tamayol, Ali, additional, Sinha, Indranil, additional, Pourquié, Olivier, additional, and Shin, Su Ryon, additional

Published
2021
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21. Engineering 3D parallelized microfluidic droplet generators with equal flow profiles by computational fluid dynamics and stereolithographic printing

Author

Kamperman, Tom, primary, Teixeira, Liliana Moreira, additional, Salehi, Seyedeh Sarah, additional, Kerckhofs, Greet, additional, Guyot, Yann, additional, Geven, Mike, additional, Geris, Liesbet, additional, Grijpma, Dirk, additional, Blanquer, Sebastien, additional, and Leijten, Jeroen, additional

Published
2020
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22. 3D Printed Tissues: 3D Printed Cartilage‐Like Tissue Constructs with Spatially Controlled Mechanical Properties (Adv. Funct. Mater. 51/2019)

Author

Melo, Bruna A. G., primary, Jodat, Yasamin A., additional, Mehrotra, Shreya, additional, Calabrese, Michelle A., additional, Kamperman, Tom, additional, Mandal, Biman B., additional, Santana, Maria H. A., additional, Alsberg, Eben, additional, Leijten, Jeroen, additional, and Shin, Su Ryon, additional

Published
2019
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23. Spatiotemporal material functionalization via competitive supramolecular complexation of avidin and biotin analogs

Author

Kamperman, Tom, Koerselman, Michelle, Kelder, Cindy, Hendriks, Jan, Crispim, João F., de Peuter, Xandra, Dijkstra, Pieter J., Karperien, Marcel, Leitjen, Jeroen, Kamperman, Tom, Koerselman, Michelle, Kelder, Cindy, Hendriks, Jan, Crispim, João F., de Peuter, Xandra, Dijkstra, Pieter J., Karperien, Marcel, and Leitjen, Jeroen

Abstract

Spatiotemporal control over engineered tissues is highly desirable for various biomedical applications as it emulates the dynamic behavior of natural tissues. Current spatiotemporal biomaterial functionalization approaches are based on cytotoxic, technically challenging, or non-scalable chemistries, which has hampered their widespread usage. Here we report a strategy to spatiotemporally functionalize (bio)materials based on competitive supramolecular complexation of avidin and biotin analogs. Specifically, an injectable hydrogel is orthogonally post-functionalized with desthiobiotinylated moieties using multivalent neutravidin. In situ exchange of desthiobiotin by biotin enables spatiotemporal material functionalization as demonstrated by the formation of long-range, conformal, and contra-directional biochemical gradients within complex-shaped 3D hydrogels. Temporal control over engineered tissue biochemistry is further demonstrated by timed presentation and sequestration of growth factors using desthiobiotinylated antibodies. The method’s universality is confirmed by modifying hydrogels with biotinylated fluorophores, peptides, nanoparticles, enzymes, and antibodies. Overall, this work provides a facile, cytocompatible, and universal strategy to spatiotemporally functionalize materials.

Published
2019

31. Microfluidics for Complex Tissue Engineering

Author

Kamperman, Tom, Henke, S.J., Karperien, Hermanus Bernardus Johannes, Developmental BioEngineering, and Faculty of Science and Technology

Subjects
METIS-321615, IR-103933
Published
2016

38. Scalable fabrication, compartmentalization and applications of living microtissues

Author

Schot, Maik, Araújo-Gomes, Nuno, van Loo, Bas, Kamperman, Tom, and Leijten, Jeroen

Abstract

Living microtissues are used in a multitude of applications as they more closely resemble native tissue physiology, as compared to 2D cultures. Microtissues are typically composed of a combination of cells and materials in varying combinations, which are dictated by the applications’ design requirements. Their applications range wide, from fundamental biological research such as differentiation studies to industrial applications such as cruelty-free meat production. However, their translation to industrial and clinical settings has been hindered due to the lack of scalability of microtissue production techniques. Continuous microfluidic processes provide an opportunity to overcome this limitation as they offer higher throughput production rates as compared to traditional batch techniques, while maintaining reproducible control over microtissue composition and size. In this review, we provide a comprehensive overview of the current approaches to engineer microtissues with a focus on the advantages of, and need for, the use of continuous processes to produce microtissues in large quantities. Finally, an outlook is provided that outlines the required developments to enable large-scale microtissue fabrication using continuous processes.

Published
2022
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39. Engineering large-scale hiPSC-derived vessel-integrated muscle-like lattices for enhanced volumetric muscle regeneration.

Author

Lee MC, Jodat YA, Endo Y, Rodríguez-delaRosa A, Zhang T, Karvar M, Al Tanoury Z, Quint J, Kamperman T, Kiaee K, Ochoa SL, Shi K, Huang Y, Rosales MP, Arnaout A, Lee H, Kim J, Ceron EL, Reyes IG, Panayi AC, Martinez AFH, Wang X, Kim KT, Moon JI, Park SG, Lee K, Calabrese MA, Hassan S, Lee J, Tamayol A, Lee L, Pourquié O, Kim WJ, Sinha I, and Shin SR

Abstract

Engineering biomimetic tissue implants with human induced pluripotent stem cells (hiPSCs) holds promise for repairing volumetric tissue loss. However, these implants face challenges in regenerative capability, survival, and geometric scalability at large-scale injury sites. Here, we present scalable vessel-integrated muscle-like lattices (VMLs), containing dense and aligned hiPSC-derived myofibers alongside passively perfusable vessel-like microchannels inside an endomysium-like supporting matrix using an embedded multimaterial bioprinting technology. The contractile and millimeter-long myofibers are created in mechanically tailored and nanofibrous extracellular matrix-based hydrogels. Incorporating vessel-like lattice enhances myofiber maturation in vitro and guides host vessel invasion in vivo, improving implant integration. Consequently, we demonstrate successful de novo muscle formation and muscle function restoration through a combinatorial effect between improved graft-host integration and its increased release of paracrine factors within volumetric muscle loss injury models. The proposed modular bioprinting technology enables scaling up to centimeter-sized prevascularized hiPSC-derived muscle tissues with custom geometries for next-generation muscle regenerative therapies., Competing Interests: Declaration of interests The authors declare no conflict of interest., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Published
2024
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40. 3D Printed Cartilage-Like Tissue Constructs with Spatially Controlled Mechanical Properties.

Author

de Melo BAG, Jodat YA, Mehrotra S, Calabrese MA, Kamperman T, Mandal BB, Santana MHA, Alsberg E, Leijten J, and Shin SR

Abstract

Developing biomimetic cartilaginous tissues that support locomotion while maintaining chondrogenic behavior is a major challenge in the tissue engineering field. Specifically, while locomotive forces demand tissues with strong mechanical properties, chondrogenesis requires a soft microenvironment. To address this challenge, 3D cartilage-like tissue is bioprinted using two biomaterials with different mechanical properties: a hard biomaterial to reflect the macromechanical properties of native cartilage, and a soft biomaterial to create a chondrogenic microenvironment. To this end, a hard biomaterial (MPa order compressive modulus) composed of an interpenetrating polymer network (IPN) of polyethylene glycol (PEG) and alginate hydrogel is developed as an extracellular matrix (ECM) with self-healing properties, but low diffusive capacity. Within this bath supplemented with thrombin, fibrinogen containing human mesenchymal stem cell (hMSC) spheroids is bioprinted forming fibrin, as the soft biomaterial (kPa order compressive modulus) to simulate cartilage's pericellular matrix and allow a fast diffusion of nutrients. The bioprinted hMSC spheroids improve viability and chondrogenic-like behavior without adversely affecting the macromechanical properties of the tissue. Therefore, the ability to print locally soft and cell stimulating microenvironments inside of a mechanically robust hydrogel is demonstrated, thereby uncoupling the micro- and macromechanical properties of the 3D printed tissues such as cartilage., Competing Interests: Conflict of Interest The authors declare no conflict of interest.

Published
2019
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