Search

Introduction: Recombinant human erythropoietin (rhEPO) has been produced in different conditions and cell lines. Identification, isolation, and purification of this protein from various sources have pivotal role in clinical applications. Hence, Clinical trials should be carried out for the identification of purity and aggregation of biological EPO. Moreover, the purification of EPO cooperates with various recommended processes has been manifested, but the strategies schemes (i.e., liquid chromatography (HPLC) and ion exchange chromatography (IEX) are often used in combination. In the current study, the quality of purification, biological modifications and the stability of rhEPO using various chromatography methods including HPLC and IEX have been assessed. Materials and Methods: rhEPO was expressed in the Chinese hamster ovary (CHO) cells and purified by the general requirements for the quality control of biological products. For the assessment of the influence of IEX in the purity pattern of rhEPO, HPLC and biological analysis were performed for 3 samples. Results: Our results revealed that the combination of 4 strategies represent confident methods for evaluating the quality of this biological medicinal product; moreover, purity and biochemical applications will yield to a relatively pure protein preparation. The activity of EPO was presented by monomeric isoform and high acid sialic purification in final product. Moreover, the determination of the biochemical reactions rate and their relationship tests were obtained by change in electrical conductivity with pH being 31.5 and 5, respectively. Conclusion: Taken together, our results indicated that different purification process based on our results can increase the accuracy of rhEPO purification. [ABSTRACT FROM AUTHOR]

Background & Objective: The current study aimed at assessing the relationship between gastritis and peptic ulcer susceptibility and inflammation-related gene polymorphisms in Iranian patients. Gastritis and peptic ulcer are common medical complications with serious outcomes on the quality of life. Inflammatory responses of gastric mucosa are associated with helicobacter pylori, but most infected patients remain asymptomatic. There is strong evidence that inflammatory response is a major part of its etiology. Methods: The current case-control study aimed atexamining genetic polymorphisms in inflammatory cytokines interleukin (IL)-4, and IL-10 using polymerase chain reaction- variable number tandem reaped (PCR-VNTR) and PCR-restriction fragment length polymorphism(RFLP) methods, respectively in 603 genotyped patients admitted to Mohammadi Hospital in Bandar Abbas, Iran(198 patients with gastritis, 84 with peptic ulcer, and 321 patients as controls). Results: No significant associations were detected in genotype and allele frequencies of IL-10 and IL-4 between the case (with gastritis and peptic ulcer) and control groups. Conclusion: In conclusion, the results of the analyses suggested that these polymorphisms may not predispose the carriers to gastritis and peptic ulcer development. [ABSTRACT FROM AUTHOR]