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450 results on '"Kakuma T"'

1. Therapeutic hypothermia can be induced and maintained using either commercial water bottles or a 'phase changing material' mattress in a newborn piglet model

Author

Iwata, S., Iwata, O., Olson, L., Kapetanakis, A., Kato, T., Evans, S., Araki, Y., Kakuma, T., Matsuishi, T., Setterwall, F., Lagercrantz, H., and Robertson, N.J.

Subjects
Hypothermia -- Care and treatment, Drinking water, Bottled -- Usage, Animal models in research -- Usage, Encephalopathy -- Care and treatment, Therapeutics, Physiological -- Usage, Therapeutics, Physiological -- Equipment and supplies
Published
2009

16. Pre‐treatment CD8+ tumour‐infiltrating lymphocyte density predicts distant metastasis after definitive treatment in patients with stage III/IV hypopharyngeal squamous cell carcinoma.

Author

Ono, T., Chitose, S., Umeno, H., Azuma, K., Kawahara, A., Akiba, J., and Kakuma, T.

Subjects
LYMPHOCYTES, METASTASIS, SQUAMOUS cell carcinoma, TUMORS, BIOMARKERS
Abstract

Objectives: Although inflammatory markers, such as the neutrophil‐to‐lymphocyte ratio, platelet‐to‐lymphocyte ratio and local immune markers have been shown to have prognostic utility, limited information is available regarding inflammatory and pre‐existing tumour‐infiltrating lymphocyte density and their association with prognosis in patients with hypopharyngeal squamous cell carcinoma. We investigated the prognostic ability of inflammatory markers and tumour‐infiltrating lymphocyte density in stage III and stage IV hypopharyngeal squamous cell carcinoma patients receiving definitive treatment. Design: Retrospective cohort study. Setting: Kurume University Hospital. Participants: Ninety‐six stage III or stage IV hypopharyngeal squamous cell carcinoma patients treated at the Kurume University Hospital between 2000 and 2014. Main outcome measures: Inflammatory markers and pre‐treatment tumour‐infiltrating lymphocyte density were examined from recorded haematologic data and immunohistochemical analysis. Results: Multivariate analyses showed that the CD8+ tumour‐infiltrating lymphocyte density was an independent predictive factor for distant metastasis and overall survival, whereas inflammatory markers, including the neutrophil‐to‐lymphocyte ratio and platelet‐to‐lymphocyte ratio, were not correlated with distant metastasis or overall survival. Conclusions: Higher pre‐treatment CD8+ tumour‐infiltrating lymphocyte density is a useful predictive biomarker for reduced distant metastasis and better prognosis. [ABSTRACT FROM AUTHOR]

Published
2018
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21. Obesity-related chronic kidney disease is associated with spleen-derived IL-10

Author

Gotoh, K., primary, Inoue, M., additional, Masaki, T., additional, Chiba, S., additional, Shiraishi, K., additional, Shimasaki, T., additional, Matsuoka, K., additional, Ando, H., additional, Fujiwara, K., additional, Fukunaga, N., additional, Aoki, K., additional, Nawata, T., additional, Katsuragi, I., additional, Kakuma, T., additional, Seike, M., additional, and Yoshimatsu, H., additional

Published
2012
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23. Randomized clinical trial of an ethanol extract of Ganoderma lucidum in men with lower urinary tract symptoms

Author

Noguchi, M., Kakuma, T., and Tomiyasu, K.

Subjects
Urinary tract infections -- Risk factors -- Diagnosis -- Drug therapy -- Research, Clinical trials -- Research -- Usage, Reishi -- Usage -- Dosage and administration -- Research, Health, Drug therapy, Diagnosis, Usage, Research, Risk factors, Dosage and administration
Abstract

Aim: A double-blind, placebo-controlled, randomized, and dose-ranging study was carried out in men with lower urinary tract symptoms (LUTS) to evaluate the safety and efficacy of an extract of Ganoderma [...]

Published
2008

26. Results of Treatment of 112 Cases of Primary CNS Lymphoma

Author

Yamanaka, R., primary, Morii, K., additional, Shinbo, Y., additional, Homma, J., additional, Sano, M., additional, Tsuchiya, N., additional, Yajima, N., additional, Tamura, T., additional, Hondoh, H., additional, Takahashi, H., additional, Kakuma, T., additional, and Tanaka, R., additional

Published
2008
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40. Hyperglycosylated HCG expression in pregnancy: Cellular origin and clinical applications

Author

Kovalevskaya, G., Kakuma, T., Schlatterer, J., and O’Connor, J.F.

Subjects
*OBSTETRICS, *PREGNANCY, *EXCRETION, *URINE
Abstract

Abstract: Employing a monoclonal antibody (B152) specific for a carbohydrate epitope found on a choriocarcinoma derived hCG, it was discovered that a similar hCG isoform is expressed during early pregnancy. This form differs from later pregnancy hCG in carbohydrate moieties. Profiling of these two hCG isoforms throughout pregnancy utilized two IRMA''s: B152–B207 (“hyperglycosylated hCG”-specific assay) and B109–B108 (an IRMA for standard intact hCG isoforms in the WHO hCG reference preparation). The WHO hCG standard was used in both assays. Values were presented as a ratio of hCG isoform concentrations (B152/B109 ratio). In early pregnancy urine concentrations of B152 hCG were significantly higher in normal pregnancy (NP) compared to early pregnancy loss (EPL). Matched serum–urine samples from the first and third trimesters revealed that the B152 hCG form is predominant in both serum and urine in the first trimester compared with the third trimester. The proportion of the B152 hCG (HhCG) form is higher in urine than in matched serum. There was a significant difference in the B152/B109 ratio between days 5 and 20 from time of embryo transfer in normally developing pregnancy versus EPL in the urine of IVF patients. In spontaneous abortion (SA) the level of B109 hCG remained higher in NP compared with SA. However, the B152/B109 ratio declined with gestational age faster in SA than in NP suggesting perhaps a different loss mechanism in SA versus EPL. The cellular origin of the different hCG glycoforms was identified by assay of cell media from cytotrophoblasts (CTBs) and syncytiotrophoblasts (STBs). Isolated CTBs expressed predominantly HhCG. The level of expression was the highest in the first trimester. STBs were the source of the less glycosylated B109 hCG isoform. Analysis of hCG glycoforms during early pregnancy can distinguish pregnancies that will fail from those that will proceed normally. Since the B152 assay does not effectively discriminate between intact HhCG and free beta HhCG (HhCGβ), a new HhCGβ assay was developed. This assay recognizes the HhCGβ which is produced by CTBs. We hypothesize that the measurement of HhCGβ may have a potential use in screening for Down syndrome and perhaps other pregnancy disorders and certain types of cancer. [Copyright &y& Elsevier]

Published
2006
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48. Organization and expression of the mouse MTH1 gene for preventing transversion mutation.

Author

Igarashi, H, Tsuzuki, T, Kakuma, T, Tominaga, Y, and Sekiguchi, M

Abstract

An enzyme, 8-oxo-7,8-dihydrodeoxyguanosine triphosphatase (8-oxo-dGTPase), is present in various organisms and plays an important role in the control of spontaneous mutagenesis. The enzyme hydrolyzes 8-oxo-dGTP, an oxidized form of dGTP, to 8-oxo-dGMP, thereby preventing the occurrence of A:T to C:G transversion, caused by misincorporation. We isolated the mouse genomic sequence encoding the enzyme and elucidated its structure. The gene, named MTH1 for mutT homologue 1, is composed of at least five exons and spans approximately 9 kilobase pairs. A genomic region containing the pseudogene was also isolated. The promoter region for the gene is GC-rich, contains many AP-1 and AP-2 recognition sequences, and lacks a typical TATA box. Primer extension and S1 mapping analyses revealed the existence of multiple transcription initiation sites, among which a major site was defined as +1. The putative promoter region was placed upstream of the chloramphenicol acetyltransferase reporter gene, and control of expression of the gene was examined by introducing the construct into mouse NIH 3T3 cells. Deletion analysis indicated that a sequence from -321 to +9 carries the basic promoter activity while an adjacent region, spanning from +352 to +525 stimulates the frequency of transcription.

Published
1997

49. Mouse MTH1 protein with 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphatase activity that prevents transversion mutation. cDNA cloning and tissue distribution.

Author

Kakuma, T, Nishida, J, Tsuzuki, T, and Sekiguchi, M

Abstract

8-Oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate (8-oxo-dGTP) is formed in the nucleotide pool of a cell during normal cellular metabolism, and when it is incorporated into DNA causes mutation. Organisms possess 8-oxo-dGTPase, an enzyme that specifically degrades 8-oxo-dGTP to 8-oxo-dGMP. We isolated cDNA for mouse 8-oxo-dGTPase, using as a probe human MTH1 (Escherichia coli mutT homolog) cDNA. The nucleotide sequence of the cDNA revealed that the mouse MTH1 protein (molecular weight of 17,896) comprises 156 amino acid residues. When the cDNA for mouse 8-oxo-dGTPase was expressed in E. coli mutT- mutant cells devoid of their own 8-oxo-dGTPase activity, an 18-kDa protein, which is cross-reactive with an anti-human MTH1 antibody, was formed. In such cells, the level of spontaneous mutation frequency that was elevated reverted to normal. High levels of 8-oxo-dGTPase activity were found in liver, thymus, and large intestine, whereas all other organs examined contained smaller amounts of the enzyme. In embryonic stem cells, an exceedingly high level of the enzyme was present.

Published
1995

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