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This research describes a project to improve the health of urban residents while simultaneously increasing the income of local rural residents. The project investment is shared by the government, village collectives (individual villagers) and investors. The project will be completed in stages, and follow-up investment will be made after the basic projects in the early stage are running smoothly to build long-term recuperation places and further transform tourist attractions. When evaluating project effects, take multiple perspectives to evaluate, and try to digitize the results.

PurposeQuantitative analysis of existing literature is conducted to compare the textual features of ethics education in science and technology under the broad theme of ethics in science and technology. On this basis, the authors reveal the research hotspots and topic evolution in this field, and propose development suggestions in conjunction with the 5W theory.Design/methodology/approachIn this paper, the authors visualize the graph and quantify the indicators in four aspects: time series, institutional collaboration, author co-authorship, and research hotspots.FindingsCompared to ethics of science and technology, the research results in the field of ethics of science and technology education are limited. There is still room for improvement in the low density of cooperation between authors and institutions. The research themes are focused on theoretical discussions and countermeasure research. At present, the reform of ethics of science and technology is still in its infancy and has not yet formed a perfect system for education and personnel training. It is necessary for research on the ethical theory of technology to provide theoretical support.Originality/valueIn the context of sustainable development strategies, it is beneficial to explore the path of pedagogical optimization of ethics of science and technology in this study. This includes the maintenance of a good research environment and the realization of a healthy development in the field of science and technology.

The ODS efficiency is in the order thiophene > dibenzothiophene > benzothiophene and may be attributed to the combined effect of HPMo and shape selectivity over Nano-TS-1.

Convolutional neural networks (CNN) has been widely used in biomedical image segmentation (BIS) tasks for its remarkable feature representation capability, and most of existing CNN-based segmentation networks leverage a down-sampling operation to achieve larger acceptance domain. However, down-sampling operations could inevitably loss the detailed information of images which is very important for the BIS task. In this paper, we propose a full-resolution biomedical image segmentation network(FRNet) that could maintain the integrated detailed information of image while keeping sufficient semantic information and large receptive field. Specifically, the basic semantic feature and non-destructive feature are employed to represent the semantic and detailed information of images, respectively. A backbone network and a new full-feature extraction branch are conducted to extract those two kinds of complementary features. Furthermore, a novel feature fusion module is designed to integrate those complementary features to achieve non-destructive description of images. Finally, in order to further improve the description ability of the integrated feature, a Densely connected Atrous Spatial Pyramid Pooling(DenseASPP) module is arranged at the end of our proposed FRNet to extract the multiscale information of images. Thorough experimental results on several available databases demonstrate the effectiveness and advancement of FRNet.

Deep-learning frameworks have been widely used in deformable image registration(DIR), which is a fundamental step in medical image analysis. In traditional deep-learning-based DIR methods, pairwise registration is conducted for each image, cooperated with a dual-input structure. They only considered that the registered images need to be as similar as possible to the target image, while ignored that all registered images to the same target image should have similar appearance too. Therefore, in this paper we add the deformation constraints between different registered images to the registration network in order to enhance the appearance consistency of the registered images and improve the registration accuracy. Specifically, a Triple-Input-Unsupervised neural Network (TIUNet) is proposed to register two source images to a common target image at the same time. Then, a novel deformation-strengthened loss is designed to simulate the deformation constraint between the two warped source images. Finally, the deformation constraints between the source images and the target image are jointly optimized to obtain the deformation field model. The experimental results on the available tissue segmentation database demonstrate that our proposed TIUNet method shows a superior performance of deformation field model over several other advanced deformable image registration algorithms. Moreover, benefited from the triple-input structure, our proposed TIUNet method could expand the available training sets naturally and is suitable for the registration task with fewer training data.

Objective. To test the biomechanical properties of 3D printed tantalum and titanium porous scaffolds. Methods. Four types of tantalum and titanium scaffolds with four alternative pore diameters, #1 (1000-700 μm), #2 (700-1000 μm), #3 (500-800 μm), and #4 (800-500 μm), were molded by selective laser melting technique, and the scaffolds were tested by scanning electronic microscope, uniaxial-compression tests, and Young’s modulus tests; they were compared with same size pig femoral bone scaffolds. Results. Under uniaxial-compression tests, equivalent stress of tantalum scaffold was 411 ± 1.43 MPa, which was significantly larger than the titanium scaffolds ( P < 0.05 ). Young’s modulus of tantalum scaffold was 2.61 ± 0.02 GPa, which was only half of that of titanium scaffold. The stress-strain curves of tantalum scaffolds were more similar to pig bone scaffolds than titanium scaffolds. Conclusion. 3D printed tantalum scaffolds with varying pore diameters are more similar to actual bone scaffolds compared with titanium scaffolds in biomechanical properties.

Pretreatment of lignocellulosic biomass using deep eutectic solvent (DES) has been demonstrated environmental and valid. Co-existing of donor and acceptor of hydrogen bond makes DES composition more complicated than traditional solvents, which limits their further scale-up utilization. Advances in biomass pretreatment using green solvent DES should excogitate efficient methodology for DES recycling. Electronically-controlled chemical methodology was first put forward to resolve recovery and recycling issue of DES lactic acid-ethylene glycol after biomass pretreatment. The methodology worked based on selectively migrating of lactate Lac

Patients with advanced prostate cancer (PCa) have poor prognosis. Circular RNAs (circRNAs) regulate biological processes in a variety of cancers, but the precise roles of circRNAs in PCa are poorly understood. Herein, we identified a novel circRNA, termed circMBOAT2 (has_circ_0007334), which was significantly overexpressed in PCa tissues and cell lines. Overexpression of circMBOAT2 was associated with high Gleason score, advanced pathological T stage, and poor prognosis. Overexpression of circMBOAT2 promoted proliferation, migration, and invasion of PCa cells in vitro, and enhanced tumorigenesis and metastasis in vivo. Mechanistically, circMBOAT2 overexpression upregulated the expression of mTOR by acting as a decoy for miR-1271-5p, resulting in the activation of the PI3K/Akt pathway, ultimately promoting the progression of PCa. Importantly, application of an inhibitor of mTOR significantly antagonized circMBOAT2-mediated PCa tumorigenesis in vivo. circMBOAT2 promotes proliferation and metastasis of PCa through miR-1271-5p/mTOR axis-mediated activation of the PI3K/Akt pathway. In summary, our findings uncover a molecular mechanism in the progression of PCa and indicate that circMBOAT2 may be a useful prognostic biomarker and therapeutic target in PCa.

Background Histone lysine demethylase 4B (KDM4B) has been implicated in various pathological processes and human diseases. Glucose metabolism is the main pattern of energy supply in cells and its dysfunction is closely related to tumorigenesis. Recent study shows that KDM4B protects against obesity and metabolic dysfunction. We realized the significant role of KDM4B in metabolism. However, the role of KDM4B in glucose metabolism remains unclear. Here, we sought to delineate the role and mechanism of KDM4B in glucose metabolism in colorectal cancer (CRC). Methods We first analyzed the role of KDM4B in glucose uptake and CRC growth. We then investigated the consequences of KDM4B inhibition on the expression of GLUT1 and AKT signaling, also explored the underlying mechanism. Finally, we detected the mechanism in vivo and assessed the potential correlation between the expression of KDM4B and CRC prognosis. Results We found that KDM4B promoted glucose uptake and ATP production by regulating the expression of GLUT1 via the AKT signaling pathway. KDM4B could interact with TRAF6 and promote TRAF6-mediated ubiquitination of AKT for AKT activation. Furthermore, we demonstrated that KDM4B was overexpressed in CRC specimens and high level of KDM4B was associated with a poor survival rate in CRC patients. Conclusions These findings reveal that KDM4B plays an important role in promoting CRC progression by enhancing glucose metabolism.

Background: Salmonellaenterica is one of the common pathogens in both humans and animals that causes salmonellosis and threatens public health all over the world. Methods and Results: Here we determined the virulence phenotypes of nine Salmonellaenterica subsp. enterica (S. enterica) isolates in vitro and in vivo, including pathogenicity to chicken, cell infection, biofilm formation and virulence gene expressions. S. Enteritidis 211 (SE211) was highly pathogenic with notable virulence features among the nine isolates. The combination of multiple virulence genes contributed to the conferring of the high virulence in SE211. Importantly, many mobile genetic elements (MGEs) were found in the genome sequence of SE211, including a virulence plasmid, genomic islands, and prophage regions. The MGEs and CRISPR-Cas system might function synergistically for gene transfer and immune defense. In addition, the neighbor joining tree and the minimum spanning tree were constructed in this study. Conclusions: This study provided both the virulence phenotypes and genomic features, which might contribute to the understanding of bacterial virulence mechanisms in Salmonella enterica subsp. enterica. The first completed genomic sequence for the high virulent S. Enteritidis isolate SE211 and the comparative genomics and phylogenetic analyses provided a preliminary understanding of S. enterica genetics and laid the foundation for further study.

The etiology and treatment of interstitial cystitis/bladder pain syndrome are still controversial. The purpose of this study is to determine the key genes and specific regulatory pathways related to it and to find potential drug-active components through integrated bioinformatics.The data set GSE11783 was downloaded from GEO database. The modules significantly related to interstitial cystitis/bladder pain syndrome were identified by weighted correlation network analysis. The genes in the key modules were analyzed by functional enrichment and protein interaction by Cytoscape software, and finally the core hub genes were screened. Furthermore, the molecular docking verification of active components and key proteins was carried out by using AutoDock Vin software.Among the 14 modules derived from WGCNA, turquoise module had the highest correlation with IC/BPS (r = 0.85, P 0.001). The genes in the module were mainly enriched in the biological processes such as the interaction between cytokines and cytokine receptors and chemokine signaling pathway. The genes in the related modules of differentially expressed genes and WGCNA traits were intersected to obtain the core hub genes. Protein-protein interaction network analysis showed that the key genes were upregulated genes CCR7 and CCL19. In terms of molecular docking, triptolide, the active component in the traditional anti-inflammatory drug Tripterygium wilfordii, can form effective molecular binding with both core hub genes.Our study identified the core hub genes CCR7 and CCL19, which acted as essential components in interstitial cystitis/bladder pain syndrome. Furthermore, CCR7 and CCL19 can form effective binding with triptolide, which will provide new insights into the development of new therapies for interstitial cystitis/bladder pain syndrome.

ObjectivesPrevious studies showed that age is the most important factor that determines the outcome after embryo transfer (ET), with either in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI), regardless whether fresh or frozen cycles. The average cumulative live birth rate (CLBR) following a single ovarian stimulation cycle in women of advanced reproductive age (≥38 years of age) has been reported to be 22.6–34.1%. The purpose of this study is to compare the CLBR after a single ovarian stimulation cycle in women of different advanced reproductive age bracket (38/39, 40/41, 42/43 years of age or older), and to explore the factors (e.g., age, type of infertility, body mass index (BMI), ovarian stimulation protocols) associated with CLBR.MethodsThis retrospective analysis included all women of advanced reproductive age (38 years or older) undergoing IVF or ICSI at authors’ institute during a period from January 1, 2016 to December 31, 2018. The study protocol was approved by the Ethics Committee of the Second Hospital of Hebei Medical University (No. 2021-P045). Subjects with underlying diseases were excluded from analysis. The last follow-up was conducted in December 2020, with minimal 2-year follow-up.ResultsThe final analysis included 826 women (40.00 ± 2.10 years of age at the time of ovarian stimulation; n = 633 and 193 for IVF-ET and ICSI-ET, respectively). The number of women in each age bracket was: 424 for 38/39 y, 226 for 40/41 y, 118 for 42/43 y, and 58 for ≥44 y. The number of transferable embryos was 2 (interquartile range: 2,4) for 38/39 y, 2 (2,3) for 40/41 y, 2 (2,3) for 42/43 y, and 2 (1.75,3) for ≥44 y. The rate of fresh embryo transfer was comparable (62.03–72.58%) among the 4 age brackets. The average CLBR following a single cycle was 26.27% in the overall study population, 32.31% for 38/39 y, 26.99% for 40/41 y, 14.4% for 42/43 y, and 3.44% for ≥44 y (P <0.001). In multivariate regression, CLBR was independently associated with younger age (OR for each year: 1.538, 95%CI: 1.193, 1.984) and higher number of transferable embryos (OR for each embryo: 1.495, 95%CI: 1.297, 1.722). CLBR differed significantly in the 38/39 group (P = 0.014), with higher rate in women receiving the Gonadotropin-releasing hormone agonist (GnRH-a) long or GnRH-a ultra-long protocols.ConclusionsForty-two years of age seemed to be a critical cutoff to achieve reasonable level of CLBR after a single ovarian stimulation cycle in women of advanced reproductive age.

The purpose of this study was to investigate the changes of resistance phenotype and plasmid-mediated quinolone resistance genes (PMQRs) in Escherichia coli (E. coli) during enrofloxacin (ENR) administration in different breeding cycles. In 2020, 983 strains of E. coli were isolated from different samples in different cycles at the broiler farm with the largest single batch of slaughter capacity in Hebei Province, China. All samples were from chicken, environmental, and human sources. The sensitivity of the isolates to various antibiotics was determined by broth microdilution method. The findings of this study include: (1) the total isolation rate of E. coli in the four cycles was 63.83% (983/1540), (2) the average resistance rate of E. coli from 1-day-old chickens to enrofloxacin was as high as 75% in each cycle, and with the use of enrofloxacin, the resistance rate of E. coli from chickens gradually increased to 100%, (3) 107 strains of E. coli randomly selected from different cycles and sources demonstrated the multi-drug resistance phenotypes. The highest resistance rate was doxycycline (100%), and the lowest was erythromycin (54.21%), (4) the detection rate of PMQRs of E. coli from chickens in different cycles were always higher than that from environmental and human. In particular, the PMQRs pollution rate of chicken seedlings in each cycle were generally higher than that of other sources, (5) We used SPSS software to analyze the Kendall rank correlation of the experimental data. The resistance of E. coli isolated from this farm to ciprofloxacin (CIP) may increase along with the increase of resistance to enrofloxacin (Kendall’s tau-b = 0.190, p = 0.021). All these data highlight the serious problem of bacterial resistance in this farm. Therefore, it is urgent to provide guidance for the prevention and control of colibacillosis and drug resistance in this farm.

Background: Salmonella enterica is one of the common pathogens in both humans and animals that causes salmonellosis and threatens public health all over the world. Methods and Results: Here we determined the virulence phenotypes of nine Salmonella enterica subsp. enterica (S. enterica) isolates in vitro and in vivo, including pathogenicity to chicken, cell infection, biofilm formation and virulence gene expressions. S. Enteritidis 211 (SE211) was highly pathogenic with notable virulence features among the nine isolates. The combination of multiple virulence genes contributed to the conferring of the high virulence in SE211. Importantly, many mobile genetic elements (MGEs) were found in the genome sequence of SE211, including a virulence plasmid, genomic islands, and prophage regions. The MGEs and CRISPR-Cas system might function synergistically for gene transfer and immune defense. In addition, the neighbor joining tree and the minimum spanning tree were constructed in this study. Conclusions: This study provided both the virulence phenotypes and genomic features, which might contribute to the understanding of bacterial virulence mechanisms in Salmonella enterica subsp. enterica. The first completed genomic sequence for the high virulent S. Enteritidis isolate SE211 and the comparative genomics and phylogenetic analyses provided a preliminary understanding of S. enterica genetics and laid the foundation for further study.

Different speciation of heavy metal ions has different toxicity level. The different speciation contents can be used as an important basis to evaluate the passivation efficiency of passivator. In this study, Ni2+, Cu2+ and Zn2+ in soil was passivated with thiol-functionalized nano-silica by solidification/stabilization technology, and the behavior of heavy metal ions in soil were explored. The result shows that, passivation time, soil moisture and passivator dosage can influence the toxicity of heavy metal ions via changing the speciation of metal ions. Passivation time and passivator dosage will promote passivation efficiency, while too low or too high soil moisture are not conducive to the passivation. The change of soil moisture will affect the surface charge distribution of soil and passivator, thus influence the migration of metal ions. According to speciation analysis, four kinds of speciation could be transferred reciprocally and the transformation will cause impact on the toxicity of heavy metal ions in soil. We explained the behavior of different metal ions based on the percentage of various speciation of heavy metal ions and inferred the transformation process of such metal ions in the passivation process. At the same time, based on Webber-Morris equation, the kinetic process during passivation process was discussed.

Ti-13Nb-13Zr alloy was fabricated by selective laser melting (SLM), and the densification, microstructure evolution, nanohardness, tensile strength and fatigue behavior of the alloy were systematically investigated. A narrow, feasible process window (laser power 325 W, scanning speed 1000 mm/s, scanning distance 0.13 mm, and layer thickness 0.03 mm) was accordingly determined. With the increase of scanning speed, the coarse acicular-shaped α′ grains changed to refined acicular-shaped α′ grains and then defect formed in the microstructure. The optimally prepared Ti-13Nb-13Zr sample had a very high hardness of 519.448 HV and tensile strength of 1106.07 MPa, which are superior higher than that prepared by traditional thermomechanical technique (280 ± 15 HV and 732 MPa). Owing to the change of BCC to HCP structure ([111] β→ [1-21-3] α′), the accumulation of dislocations and the finer grains, the SLM-processed samples exhibited higher fatigue strength than that the alloy processed by cast and was commensurate with the cast Ti-6Al-4 V. Moreover, the influences of phase transition on fatigue behavior have been discussed carefully.

Recent whole brain mapping projects are collecting large-scale 3D images using powerful and informative modalities, such as STPT, fMOST, VISoR, or MRI. Registration of these multi-dimensional whole-brain images onto a standard atlas is essential for characterizing neuron types and constructing brain wiring diagrams. However, cross-modality image registration is challenging due to intrinsic variations of brain anatomy and artifacts resulted from different sample preparation methods and imaging modalities. We introduced a cross-modality registration method, called mBrainAligner, which uses coherent landmark mapping as well as deep neural networks to align whole mouse brain images to the standard Allen Common Coordinate Framework atlas. We also built a single cell resolution atlas using the fMOST modality, and used our method to generate whole brain map of 3D full single neuron morphology and neuron cell types.

Recent whole-brain mapping projects are collecting large-scale three-dimensional images using modalities such as serial two-photon tomography, fluorescence micro-optical sectioning tomography, light-sheet fluorescence microscopy, volumetric imaging with synchronous on-the-fly scan and readout or magnetic resonance imaging. Registration of these multi-dimensional whole-brain images onto a standard atlas is essential for characterizing neuron types and constructing brain wiring diagrams. However, cross-modal image registration is challenging due to intrinsic variations of brain anatomy and artifacts resulting from different sample preparation methods and imaging modalities. We introduce a cross-modal registration method, mBrainAligner, which uses coherent landmark mapping and deep neural networks to align whole mouse brain images to the standard Allen Common Coordinate Framework atlas. We build a brain atlas for the fluorescence micro-optical sectioning tomography modality to facilitate single-cell mapping, and used our method to generate a whole-brain map of three-dimensional single-neuron morphology and neuron cell types.

Additional file 1 : Figure S1. A BrdUrd incorporation into DNA and DNA content in nuclei were determined by flow cytometry analysis in KDM4B-overexpressed LoVo cells. B Intracellular glucose uptake was evaluated by 2-NBDG, a fluorescently tagged glucose derivative in KDM4B-depressed SW620 cells (siKDM4B 1#/2#) and KDM4B-overexpressed SW620 cells (KDM4B). C GSE9348 RNA sequencing from GEO database was performed in CRC tissues and normal tissues. Pathway enrichment analysis revealed that Glucose transport pathway was more correlated in CRC tissues. Figure S2. A The expression of GLUT1 and GLUT2 in KDM4B-depressed LoVo cells in mRNA levels. B and C The expression of GLUT1 was detected in KDM4B-depressed LoVo/SW620 cells transfected with siControl and siKDM4B 1#/2#. D and E The expression of GLUT1 was detected in KDM4B-overexpressed LoVo/HCT116 cells transfected with KDM4B plasmid. Figure S3. A The phosphorylation of AKT at Thr308 and Ser 473 and the expression of GLUT1 was detected in KDM4B-depressed HCT116 cells. B The membrane fractions, cytoplasm fractions and whole cell extracts were collected in KDM4B-depressed SW620 cells to measure the phosphorylation of AKT at Thr308 and Ser 473. C BrdUrd incorporation into DNA and DNA content in nuclei were determined by flow cytometry analysis in KDM4B-overexpressed LoVo cells treated with/without LY294002. D Intracellular glucose uptake was evaluated by 2-NBDG in KDM4B-overexpressed LoVo cells treated with/without LY294002.

In this study, selective laser melting (SLM) of pure tantalum (Ta) was systematically investigated, with emphasis on densification, microstructure and mechanical properties of Ta specimen. The high laser scanning speed resulted in micropores and discontinuous scan tracks, owing to the elevated instability of the liquid induced by Marangoni convection and the balling effect. However, the interlayer thermal microcracks were produced at a low scanning speed, due to the thermal stress and balling effect. The microhardness and tensile strengths of the optimally SLM-processed Ta parts were improved to 445 HV and 739 MPa, respectively, which were considerably higher than specimens processed by cast (110 HV and 205 MPa) or powder metallurgy (120 HV and 310 MPa) method, due to the fine-grain strengthening. The fracture morphology of the tensile-failed SLM-processed specimens showed that the porosities and incompletely melted particles are responsible for the fracture of porous sample. While for dense sample, cleavage fracture and minor ductile fracture both account for the fracture. And the failure mechanisms were discussed. The reduced coefficient of friction of 0.3 and lowest wear rate of 7.1×10 −3 mm 3 N −1 m −1 in dry sliding wear tests were obtained for the optimally prepared Ta parts due to the formed adhesion of hardened tribolayers.

Registration is a critical step in many biomedical image analysis tasks. In this work, we propose a novel cascaded 3D U-Net with dilated convolution for volumetric image registration. We implement a coarse-to-fine registration strategy that commonly used in traditional registration methods by cascading two 3D U-Net based networks. In the first registration stage, a 3D U-Net network is employed to predict the large and coarse displacements. In order to capture more precise pixel-level displacement field, in the second stage, we propose to substitute the down-sampling operations in the encoding stage of 3D U-Net by dilated convolutions. As result, the resolution of feature map can be maintained as well as its field-of-view and semantic information. In addition, to obtain a more parameter-efficient network that is essential for volumetric image analysis, we replace the 3D convolution kernel by 3D dense separated convolution (3DDSC) module. We evaluated the proposed method on two commonly used datasets, and the results demonstrated that our method could obtain better performance than state-of-the-art image registration algorithms with short execution time.

Background Cellular senescence is a state of irreversible cell growth arrest and senescence cells permanently lose proliferation potential. Induction of cellular senescence might be a novel therapy for cancer cells. TRIB2 has been reported to participate in regulating proliferation and drug resistance of various cancer cells. However, the role of TRIB2 in cellular senescence of colorectal cancer (CRC) and its molecular mechanism remains unclear. Methods The expression of TRIB2 in colorectal cancer tissues and adjacent tissues was detected by immunohistochemistry and RT-PCR. The growth, cell cycle distribution and cellular senescence of colorectal cancer cells were evaluated by Cell Counting Kit-8 (CCK8) assay, flow cytometry detection and senescence-associated β-galactosidase staining, respectively. Western blot, RT-PCR and luciferase assay were performed to determine how TRIB2 regulates p21. Immunoprecipitation (IP) and chromatin-immunoprecipitation (ChIP) were used to investigate the molecular mechanisms. Results We found that TRIB2 expression was elevated in CRC tissues compared to normal adjacent tissues and high TRIB2 expression indicated poor prognosis of CRC patients. Functionally, depletion of TRIB2 inhibited cancer cells proliferation, induced cell cycle arrest and promoted cellular senescence, whereas overexpression of TRIB2 accelerated cell growth, cell cycle progression and blocked cellular senescence. Further studies showed that TRIB2 physically interacted with AP4 and inhibited p21 expression through enhancing transcription activities of AP4. The rescue experiments indicated that silencing of AP4 abrogated the inhibition of cellular senescence induced by TRIB2 overexpression. Conclusion These data demonstrate that TRIB2 suppresses cellular senescence through interaction with AP4 to down-regulate p21 expression. Therefore, TRIB2 could be a potential target for CRC treatment. Electronic supplementary material The online version of this article (10.1186/s12943-018-0922-x) contains supplementary material, which is available to authorized users.

At present, China's central heating industry is developing rapidly. It is an important direction for the transformation and upgrading of the energy industry to use the advantages of advanced information and communication technology and Internet platform to promote the intelligent energy production, promote the interconnection of various types of energy flow network and the coordinated transformation of various energy forms. In the framework of the era of industrial Internet of Things, the introduction of "Internet +" technology helps to improve the production, operation, management and service level of central heating system from the system engineering level. The "Internet +" intelligent heating system in the field of central heating integrates heating physical system and information system deeply, forming a new application mode of "digital twin". It takes "heat source -- heat network -- heat station -- user heat transfer" as the main line and adopts B/S architecture. It consists of data perception layer, data acquisition layer, data access layer, business logic layer, presentation layer and terminal access layer. In a "Internet +" intelligent heating platform demonstration construction applications, through the construction of entire network hydraulic balance analysis, the source network integration of collaborative scheduling, energy consumption statistics and analysis, management fees management, customer service management subsystem module, such as the heating index to reduce energy consumption, operating cost savings, improve work efficiency and service quality improvement has made significant achievements.

BACKGROUND Reactive extraction is a promising technique for the recovery of amino acids from aqueous solutions. The reactive extraction of L-valine with di(2-ethylhexyl) phosphate acid (D2EHPA) has been studied in this paper. An expression of equilibrium distribution ratio (D) was proposed to describe the experimental data. RESULTS The diluents, equilibrium pH value (pHeq), the concentrations of L-valine and D2EHPA were found to be the key factors that affected D. The highest D was observed at pHeq around 2.6 with 1-octanol as diluent. D value increased with the increase of the concentration of D2EHPA. In contrast, as the increase of the concentration of L-valine, D value decreased. The extraction mechanism was analyzed by using Fourier infrared spectra. The reactive extraction mechanism of L-valine with D2EHPA was mainly via proton-transfer and ion-exchange process. By fitting the experimental data, (1:1) complex of L-valine with D2EHPA was deduced. The comparison of theoretical D values with the experimental data verified the reliability of the proposed mechanism equations. CONCLUSIONS L-valine was efficiently extracted from aqueous solutions using D2EHPA in 1-octanol, which provide preliminary practical information and theoretical basis for the separation of L-valine, as well as its purification and recovery.

Based on the system coupling mechanism, a three-system coupling model of energy, economy and environment is constructed. Based on the panel data of Hebei Province from 2010 to 2019, the empirical analysis of the development status of the three systems of energy, economy and environment in Hebei Province. The results show that the coupling degree of the three systems of energy, economy and environment in Hebei Province generally shows an upward trend, and the coupling degree is consistent with the coordination degree of energy-economy, energy-environment and economy-environment. The coordinated development of each binary system is the basic guarantee for the increase in the coupling of the ternary system.