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367 results on '"Kaithwas, Gaurav"'

1. Antiproliferative effect of indeno[1,2-d]thiazolo[3,2-a]pyrimidine analogues on IL-6 mediated STAT3 and role of the apoptotic pathway in albino Wistar rats of ethyl carbamate-induced lung carcinoma: In-silico, In-vitro, and In-vivo study

Author

Sonkar, Archana Bharti, Verma, Abhishek, Yadav, Sneha, Kumar, Rohit, Singh, Jyoti, Keshari, Amit K., Rani, Soniya, Kumar, Anurag, Kumar, Dharmendra, Shrivastava, Neeraj Kumar, Rastogi, Shubham, Alamoudi, Mariam K., Ansari, Mohd Nazam, Saeedan, Abdulaziz S., Kaithwas, Gaurav, and Saha, Sudipta

Published
2024
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8. International Consensus Based Review and Recommendations for Minimum Reporting Standards in Research on Transcutaneous Vagus Nerve Stimulation (Version 2020).

Author

Farmer, Adam, Strzelczyk, Adam, Finisguerra, Alessandra, Gourine, Alexander, Gharabaghi, Alireza, Hasan, Alkomiet, Burger, Andreas, Jaramillo, Andrés, Mertens, Ann, Majid, Arshad, Verkuil, Bart, Badran, Bashar, Ventura-Bort, Carlos, Gaul, Charly, Beste, Christian, Warren, Christopher, Quintana, Daniel, Hämmerer, Dorothea, Freri, Elena, Frangos, Eleni, Tobaldini, Eleonora, Kaniusas, Eugenijus, Rosenow, Felix, Capone, Fioravante, Panetsos, Fivos, Ackland, Gareth, Kaithwas, Gaurav, OLeary, Georgia, Genheimer, Hannah, Jacobs, Heidi, Van Diest, Ilse, Schoenen, Jean, Redgrave, Jessica, Fang, Jiliang, Deuchars, Jim, Széles, Jozsef, More, Kaushik, Vonck, Kristl, Steenbergen, Laura, Vianna, Lauro, McTeague, Lisa, Ludwig, Mareike, Veldhuizen, Maria, De Couck, Marijke, Casazza, Marina, Keute, Marius, Bikson, Marom, Andreatta, Marta, DAgostini, Martina, Weymar, Mathias, Betts, Matthew, Prigge, Matthias, Kaess, Michael, Roden, Michael, Thai, Michelle, Schuster, Nathaniel, Montano, Nicola, Hansen, Niels, Kroemer, Nils, Rong, Peijing, Fischer, Rico, Howland, Robert, Sclocco, Roberta, Sellaro, Roberta, Garcia, Ronald, Bauer, Sebastian, Gancheva, Sofiya, Stavrakis, Stavros, Kampusch, Stefan, Deuchars, Susan, Wehner, Sven, Laborde, Sylvain, Usichenko, Taras, Polak, Thomas, Zaehle, Tino, Borges, Uirassu, Teckentrup, Vanessa, Jandackova, Vera, Napadow, Vitaly, Koenig, Julian, and Thayer, Julian

Subjects
guidelines & recommendations, minimum reporting standards, transcutaneous auricular vagus nerve stimulation, transcutaneous cervical vagus nerve stimulation, transcutaneous vagus nerve stimulation
Abstract

Given its non-invasive nature, there is increasing interest in the use of transcutaneous vagus nerve stimulation (tVNS) across basic, translational and clinical research. Contemporaneously, tVNS can be achieved by stimulating either the auricular branch or the cervical bundle of the vagus nerve, referred to as transcutaneous auricular vagus nerve stimulation(VNS) and transcutaneous cervical VNS, respectively. In order to advance the field in a systematic manner, studies using these technologies need to adequately report sufficient methodological detail to enable comparison of results between studies, replication of studies, as well as enhancing study participant safety. We systematically reviewed the existing tVNS literature to evaluate current reporting practices. Based on this review, and consensus among participating authors, we propose a set of minimal reporting items to guide future tVNS studies. The suggested items address specific technical aspects of the device and stimulation parameters. We also cover general recommendations including inclusion and exclusion criteria for participants, outcome parameters and the detailed reporting of side effects. Furthermore, we review strategies used to identify the optimal stimulation parameters for a given research setting and summarize ongoing developments in animal research with potential implications for the application of tVNS in humans. Finally, we discuss the potential of tVNS in future research as well as the associated challenges across several disciplines in research and clinical practice.

Published
2020

13. Harnessing Potential of ω-3 Polyunsaturated Fatty Acid with Nanotechnology for Enhanced Breast Cancer Therapy: A Comprehensive Investigation into ALA-Based Liposomal PTX Delivery.

Author

Kumar, Rohit, Kumar, Anurag, Kumar, Dharmendra, Yadav, Sneha, Shrivastava, Neeraj Kumar, Singh, Jyoti, Sonkar, Archana Bharti, Verma, Pratibha, Arya, Dilip Kumar, Kaithwas, Gaurav, Agrarwal, Ashish Kumar, and Singh, Sanjay

Subjects
CANCER cell culture, FOURIER transform infrared spectroscopy, UNSATURATED fatty acids, ALPHA-linolenic acid, LIPOSOMES, PHARMACOKINETICS
Abstract

Our hypothesis posited that incorporating alpha-linolenic acid (ALA) into liposomes containing Paclitaxel (PTX) could augment cellular uptake, decrease the therapeutic dosage, and alleviate PTX-related side effects. Our investigation encompassed characterization of the liposomal formulation, encompassing aspects like particle size, surface morphology, chemical structure, drug release kinetics, and stability. Compatibility studies were performed through Fourier transform infrared spectroscopy (FTIR). By utilizing the Box–Behnken design (BBD), we developed ALA-based liposomes with satisfactory particle size and entrapment efficiency. It is noteworthy that ALA incorporation led to a slight increase in particle size but did not notably affect drug entrapment. In vitro drug release assessments unveiled a sustained release pattern, with ALA-PTX liposomes demonstrating release profiles comparable to PTX liposomes. Morphological examinations confirmed the spherical structure of the liposomes, indicating that substituting ALA with phosphatidylcholine did not alter the physicochemical properties. Cellular uptake investigations showcased enhanced uptake of ALA-based liposomes in contrast to PTX liposomes, likely attributed to the heightened fluidity conferred by ALA. Efficacy against MCF-7 cells demonstrated concentration-dependent reductions in cell viability, with ALA-PTX liposomes exhibiting the lowest IC50 value. Morphological analysis confirmed apoptotic changes in cells treated with all formulations, with ALA-PTX liposomes eliciting more pronounced changes, indicative of enhanced anticancer efficacy. [ABSTRACT FROM AUTHOR]

Published
2024
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14. LC-mHTT-AN2 oversees lactate transport, hypoxia, and glucose homeostasis through dual regulation of MCT-1/4 in the tumor microenvironment

Author

Yadav, Sneha, primary, Singh, Jyoti, additional, Kumar, Rohit, additional, Sonkar, Archana Bharti, additional, Kumar, Anurag, additional, Kumar, Dharmendra, additional, Alamoudi, Mariam K., additional, Ansari, Mohd Nazam, additional, Saeedan, Abdulaziz S., additional, Mukherjee, Alok, additional, and Kaithwas, Gaurav, additional

Published
2024
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18. Novel carbamodithioate regulates cellular hypoxia through chemical activation of prolyl hydroxylase‐2 for breast cancer chemoprevention.

Author

Rastogi, Shubham, Ansari, Mohd Nazam, Saeedan, Abdulaziz S., Yadav, Sachin, Kumar, Dinesh, Singh, Sunil Kumar, Mukerjee, Alok, Singh, Manjari, and Kaithwas, Gaurav

Subjects
MAMMARY gland cancer, CANCER chemoprevention, ACTIVATION (Chemistry), BREAST cancer, HYPOXEMIA, METABOLOMICS
Abstract

Inhibition of prolylhydroxylase‐2 (PHD‐2) in both normoxic and hypoxic cells is a critical component of solid tumours. The present study aimed to identify small molecules with PHD‐2 activation potential. Virtually screening 4342 chemical compounds for structural similarity to R59949 and docking with PHD‐2. To find the best drug candidate, hits were assessed for drug likeliness, antihypoxic and antineoplastic potential. The selected drug candidate's PHD‐2 activation, cytotoxic and apoptotic potentials were assessed using 2‐oxoglutarate, MTT, AO/EtBr and JC‐1 staining. The drug candidate was also tested for its in‐vivo chemopreventive efficacy against DMBA‐induced mammary gland cancer alone and in combination with Tirapazamine (TPZ). Virtual screening and 2‐oxoglutarate assay showed BBAP‐6 as lead compound. BBAP‐6 exhibited cytotoxic and apoptotic activity against ER+ MCF‐7. In carmine staining and histology, BBAP‐6 alone or in combination with TPZ restored normal surface morphology of the mammary gland after DMBA produced malignant alterations. Immunoblotting revealed that BBAP‐6 reduced NF‐κB expression, activated PHD‐2 and induced intrinsic apoptotic pathway. Serum metabolomics conducted with 1H NMR confirmed that BBAP‐6 prevented HIF‐1α and NF‐κB‐induced metabolic changes in DMBA mammary gland cancer model. In a nutshell, it can be concluded that BBAP‐6 activates PHD‐2 and exhibits anticancer potential. [ABSTRACT FROM AUTHOR]

Published
2024
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19. Novel furan chalcone modulates PHD-2 induction to impart antineoplastic effect in mammary gland carcinoma.

Author

Rastogi, Shubham, Ansari, Mohd Nazam, Saeedan, Abdulaziz S., Singh, Sunil Kumar, Mukerjee, Alok, and Kaithwas, Gaurav

Subjects
MAMMARY glands, MAMMARY gland cancer, CHALCONE, NUCLEAR fragmentation, CARCINOMA in situ, ANTHRACENE
Abstract

Normoxic inactivation of prolyl hydroxylase-2 (PHD-2) in tumour microenvironment paves the way for cancer cells to thrive under the influence of HIF-1a and NF-κB. Henceforth, the present study is aimed to identify small molecule activators of PHD-2. A virtual screening was conducted on a library consisting of 265,242 chemical compounds, with the objective of identifying molecules that exhibit structural similarities to the furan chalcone scaffold. Further, PHD-2 activation potential of screened compound was determined using in vitro 2-oxoglutarate assay. The cytotoxic activity and apoptotic potential of screened compound was determined using various staining techniques, including 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, 4',6-diamidino-2-phenylindole (DAPI), 1,1',3,3'-tetraethylbenzimi-dazolylcarbocyanine iodide (JC-1), and acridine orange/ethidium bromide (AO/EB), against MCF-7 cells. 7,12-Dimethylbenz[a]anthracene (DMBA) model of mammary gland cancer was used to study the in vivo antineoplastic efficacy of screened compound. [(E)-1-(4-fluorophenyl)- 3-(furan-2-yl) prop-2-en-1-one] (BBAP-7) was screened and validated as a PHD-2 activator by an in vitro 2-oxo-glutarate assay. The IC50 of BBAP-7 on MCF-7 cells is 18.84 μM. AO/EB and DAPI staining showed nuclear fragmentation, blebbing and condensation in MCF-7 cells following BBAP-7 treatment. The red-to-green intensity ratio of JC-1 stained MCF-7 cells decreased after BBAP-7 treatment, indicating mitochondrial-mediated apoptosis. DMBA caused mammary gland dysplasia, duct hyperplasia and ductal carcinoma in situ. Carmine staining, histopathology, and scanning electron microscopy demonstrated that BBAP-7, alone or with tirapazamine, restored mammary gland surface morphology and structural integrity. Additionally, BBAP-7 therapy significantly reduced oxidative stress and glycolysis. The findings reveal that BBAP-7 activates PHD-2, making it a promising anticancer drug. [ABSTRACT FROM AUTHOR]

Published
2024
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33. Hypoxia induced lactate acidosis modulates tumor microenvironment and lipid reprogramming to sustain the cancer cell survival

Author

Singh, Lakhveer, primary, Nair, Lakshmi, additional, Kumar, Dinesh, additional, Arora, Mandeep Kumar, additional, Bajaj, Sakshi, additional, Gadewar, Manoj, additional, Mishra, Shashank Shekher, additional, Rath, Santosh Kumar, additional, Dubey, Amit Kumar, additional, Kaithwas, Gaurav, additional, Choudhary, Manjusha, additional, and Singh, Manjari, additional

Published
2023
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48. Correction to “Colloidal Vesicular System of Inositol Hexaphosphate to Counteract DMBA Induced Dysregulation of Markers Pertaining to Cellular Proliferation/Differentiation and Inflammation of Epidermal Layer in Mouse Model”

Author

Arya, Malti, primary, Tiwari, Prakash, additional, Tripathi, Chandra Bhushan, additional, Parashar, Poonam, additional, Singh, Mahendra, additional, Sinha, Priyam, additional, Yadav, Narayan P., additional, Kaithwas, Gaurav, additional, Gupta, Krishna P., additional, and Saraf, Shubhini A., additional

Published
2022
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