8 results on '"Kai-hong Zang"'
Search Results
2. Quercetin Attenuates Visceral Hypersensitivity and 5-Hydroxytryptamine Availability in Postinflammatory Irritable Bowel Syndrome Rats: Role of Enterochromaffin Cells in the Colon
- Author
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Xiao Zuo, Hong yan Qin, Zhaoxiang Bian, Kai hong Zang, and Xin An Wu
- Subjects
Male ,0301 basic medicine ,Serotonin ,medicine.medical_specialty ,Colon ,Enteroendocrine cell differentiation ,Medicine (miscellaneous) ,Nerve Tissue Proteins ,Enteroendocrine cell ,Irritable Bowel Syndrome ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Internal medicine ,Threshold of pain ,Basic Helix-Loop-Helix Transcription Factors ,Enterochromaffin Cells ,medicine ,Animals ,Humans ,heterocyclic compounds ,Rats, Wistar ,Irritable bowel syndrome ,Homeodomain Proteins ,030109 nutrition & dietetics ,Nutrition and Dietetics ,business.industry ,Visceral pain ,Visceral Pain ,Tryptophan hydroxylase ,medicine.disease ,Rats ,Disease Models, Animal ,Endocrinology ,chemistry ,030220 oncology & carcinogenesis ,Trans-Activators ,Enterochromaffin cell ,Quercetin ,medicine.symptom ,business - Abstract
Intestinal enterochromaffin (EC) cell hyperplasia and increased 5-hydroxytryptamine (5-HT) availability play key roles in the pathogenesis of abdominal hypersensitivity of irritable bowel syndrome (IBS). This study aims to study the effect of quercetin on visceral pain and 5-HT availability in postinflammatory IBS (PI-IBS) rats. PI-IBS model rats were administered quercetin by gavage at doses of 5, 10, and 20 mg/kg for 14 days. Compared with normal rats, the visceral pain threshold of PI-IBS rats was markedly decreased and the abdominal motor response to colon distension was markedly increased. The EC cell count and 5-HT level, as well as tryptophan hydroxylase (TPH) protein, were all significantly elevated in PI-IBS rats, while the 5-HT reuptake transporter (serotonin transporter) was reduced. Genes that are responsible for enteroendocrine cell differentiation, that is, Ngn3 and pdx1, were significantly increased in the PI-IBS group. Quercetin treatment markedly elevated the pain threshold pressure and decreased the visceral motor response of PI-IBS animals; and EC cell density and 5-HT level, as well as TPH expression, in the PI-IBS group were all reduced by quercetin. Quercetin treatment also significantly reduced colonic expression of Ngn3 and pdx1 of PI-IBS. Findings from the present study indicated that the analgesic effect of quercetin on PI-IBS may result from reduction of 5-HT availability in the colon, and the regulatory role of quercetin in endocrine progenitors may contribute to reduced EC cells.
- Published
- 2019
3. Astragaloside IV Attenuates Trinitrobenzene Sulphonic Acid (TNBS)-Induced Colitis via Improving Mucosal Barrier Function: Role of Goblet Cells
- Author
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Qing-lin Ma, Kai-hong Zang, Hong-yan Qin, Yuan Ren, and Hai-Jing Duan
- Subjects
Pharmacology ,Astragaloside IV ,Chemistry ,Barrier function ,Tnbs colitis - Published
- 2018
4. Enterochromaffin cell hyperplasia in the gut: Factors, mechanism and therapeutic clues
- Author
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Zhaoxiang Bian, Hoi Leong Xavier Wong, Xun Li, Kai hong Zang, and Hong yan Qin
- Subjects
Cell type ,Serotonin ,Colon ,Cell ,Inflammation ,Biology ,Infections ,General Biochemistry, Genetics and Molecular Biology ,Irritable Bowel Syndrome ,Stress, Physiological ,medicine ,Enterochromaffin Cells ,Animals ,Humans ,General Pharmacology, Toxicology and Pharmaceutics ,Progenitor cell ,Irritable bowel syndrome ,Hyperplasia ,General Medicine ,medicine.disease ,Intestines ,medicine.anatomical_structure ,Enterochromaffin cell ,Cancer research ,Stem cell ,medicine.symptom - Abstract
Enterochromaffin (EC) cell is the main cell type that responsible for 5-hydroxytryptamine (5-HT) synthesis, storage and release of the gut. Intestinal 5-HT play a key role in visceral sensation, intestinal motility and permeability, EC cell hyperplasia and increased 5-HT bioavailability in the gut have been found to be involved in the symptoms generation of irritable bowel syndrome and inflammatory bowel disease. EC cells originate from intestinal stem cells, the interaction between proliferation and differentiation signals on intestinal stem cells enable EC cell number to be regulated in a normal level. This review focuses on the impact factors, pathogenesis mechanisms, and therapeutic clues for intestinal EC cells hyperplasia, and showed that EC cell hyperplasia was observed under the condition of physiological stress, intestinal infection or intestinal inflammation, the disordered proliferation and/or differentiation of intestinal stem cells as well as their progenitor cells all contribute to the pathogenesis of intestinal EC cell hyperplasia. The altered intestinal niche, i.e. increased corticotrophin releasing factor (CRF) signal, elevated nerve growth factor (NGF) signal, and Th2-dominant cytokines production, has been found to have close correlation with intestinal EC cell hyperplasia. Currently, CRF receptor antagonist, nuclear factor-κB inhibitor, and NGF receptor neutralizing antibody have been proved useful to attenuate intestinal EC cell hyperplasia, which may provide a promising clue for the therapeutic strategy in EC cell hyperplasia related diseases.
- Published
- 2019
5. Oridonin Alleviates Visceral Hyperalgesia in a Rat Model of Postinflammatory Irritable Bowel Syndrome: Role of Colonic Enterochromaffin Cell and Serotonin Availability
- Author
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Zhi Rao, Yun-Yun Shao, Xiao Zuo, Kai-hong Zang, and Hongyan Qin
- Subjects
Male ,medicine.medical_specialty ,Serotonin ,Colon ,medicine.medical_treatment ,Medicine (miscellaneous) ,Irritable Bowel Syndrome ,Rats, Sprague-Dawley ,03 medical and health sciences ,Interferon-gamma ,0302 clinical medicine ,Internal medicine ,Enterochromaffin Cells ,Medicine ,Animals ,Humans ,Interferon gamma ,Nutrition and Dietetics ,business.industry ,Tumor Necrosis Factor-alpha ,Interleukin ,Tryptophan hydroxylase ,Rats ,Disease Models, Animal ,Cytokine ,Endocrinology ,Hyperalgesia ,030220 oncology & carcinogenesis ,Enterochromaffin cell ,030211 gastroenterology & hepatology ,Tumor necrosis factor alpha ,Interleukin-4 ,medicine.symptom ,business ,Diterpenes, Kaurane ,medicine.drug - Abstract
The aim of this present study was to investigate the effect of oridonin on visceral hyperalgesia and colonic serotonin availability in a rat model of trinitrobenzenesulfonic acid-induced postinflammatory irritable bowel syndrome (PI-IBS). Rats were randomly divided into five groups: normal control, PI-IBS model, PI-IBS+low-dose oridonin (5 mg/kg), PI-IBS+median-dose oridonin (10 mg/kg), and PI-IBS+high-dose oridonin (20 mg/kg). Rats in control and model groups were orally administered with water by gavage, whereas rats in oridonin-treated groups were orally administered with different dosages of oridonin, and drugs were given for 14 consecutive days. Compared with the control group, the pain threshold pressure was significantly reduced in PI-IBS rats. The colonic enterochromaffin (EC) cell number, serotonin content, and the protein expression of tryptophan hydroxylase (TPH) were markedly increased and the protein expression of serotonin reuptake transporter was significantly decreased in PI-IBS rats. The spleen index in PI-IBS rats was decreased, and the levels of tumor necrosis factor (TNF)-α, interferon (IFN)-γ, interleukin (IL)-4, and IL-13 in the colon of PI-IBS rats were also markedly decreased. Oridonin treatment dose dependently increased pain threshold pressure, and markedly decreased colon EC cell numbers, TPH expression, and serotonin content in PI-IBS rats. Oridonin treatment also significantly increased the spleen index as well as the levels of TNF-α, IFN-γ, IL-4, and IL-13 in the colon of PI-IBS rats. Results of this study demonstrate that the analgesic effect of oridonin in PI-IBS rats is associated with reduced colonic EC cell hyperplasia and 5-HT availability, the regulatory effect of oridonin on colonic cytokine production may be correlated with its effect on colonic EC cell number.
- Published
- 2016
6. Magnolol inhibits colonic motility through down-regulation of voltage-sensitive L-type Ca2+ channels of colonic smooth muscle cells in rats
- Author
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Yu Huang, Chengyuan Lin, Zhijun Yang, Joseph J.Y. Sung, Zhaoxiang Bian, Fung Ping Leung, Kai-Hong Zang, Hong-Xi Xu, Xu-Dong Tang, Aiping Lu, Man Zhang, and Jialie Luo
- Subjects
Male ,Carbachol ,Contraction (grammar) ,Calcium Channels, L-Type ,Stereochemistry ,Colon ,Myocytes, Smooth Muscle ,Pharmaceutical Science ,Down-Regulation ,Lignans ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,Drug Discovery ,medicine ,Animals ,5-HT receptor ,Pharmacology ,Biphenyl Compounds ,Muscle, Smooth ,Smooth muscle contraction ,Calcium Channel Blockers ,Bay K8644 ,Magnolol ,Acetylcholine ,Rats ,Complementary and alternative medicine ,chemistry ,Magnolia ,Tetrodotoxin ,Biophysics ,Molecular Medicine ,medicine.drug ,Drugs, Chinese Herbal ,Muscle Contraction - Abstract
This study aimed to investigate the effect of magnolol (5,5'-diallyl-2,2'-biphenyldiol) on contraction in distal colonic segments of rats and the underlying mechanisms. Colonic segments were mounted in organ baths for isometric force measurement. Whole-cell voltage-sensitive L-type Ca(2+) currents were recorded on isolated single colonic smooth muscle cells using patch-clamp technique. The spontaneous contractions and acetylcholine (ACh)- and Bay K 8644-induced contractions were inhibited by magnolol (3-100 μM). In the presence of Bay K8644 (100 nM), magnolol (10-100 μM) inhibited the contraction induced by 10 μM ACh. By contrast, tetrodotoxin (100 nM) and Nώ-nitro-L-arginine methyl ester (L-NAME 100 μM) did not change the inhibitory effect of magnolol (10 μM). In addition, magnolol (3-100 μM) inhibited the L-type Ca(2+) currents. The present results suggest that magnolol inhibits colonic smooth muscle contraction through downregulating L-type Ca(2+) channel activity.
- Published
- 2013
7. Anticolitis activity of chinese herbal formula Yupingfeng powder via regulating colonic enterochromaffin cells and serotonin
- Author
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Kai-hong Zang, Hongyan Qin, Zhi Rao, and Guo-qiang Zhang
- Subjects
Diarrhea ,Male ,Serotonin ,medicine.medical_specialty ,Colon ,Inflammation ,Weight Gain ,Gastroenterology ,Random Allocation ,Gastrointestinal Agents ,Thinness ,Internal medicine ,Enterochromaffin Cells ,medicine ,Animals ,Pharmacology (medical) ,Colitis ,ulcerative colitis ,Peroxidase ,enterochromaffin cell ,Pharmacology ,Mice, Inbred BALB C ,Gastrointestinal agent ,Hyperplasia ,Dose-Response Relationship, Drug ,biology ,business.industry ,Anti-Inflammatory Agents, Non-Steroidal ,Colonic inflammation ,medicine.disease ,Ulcerative colitis ,Disease Models, Animal ,Myeloperoxidase ,biology.protein ,Enterochromaffin cell ,Serotonin Antagonists ,Powders ,medicine.symptom ,business ,Research Article ,Drugs, Chinese Herbal - Abstract
Objective: To investigate whether traditional Chinese herbal formula Yupingfeng (YPF) powder has an anti-inflammatory effect on colonic inflammation, and to explore the mechanism involved. Materials and Methods: YPF powder was orally administrated to trinitrobenzene sulfonic acid (TNBS)-induced colitis mice at the dose of 3, 6, and 12 g/kg/d for 7 consecutive days. Body weight, stool consistency, histopathological score, and myeloperoxidase (MPO) activity were tested to evaluate the effect of YPF powder on colonic inflammation while colonic enterochromaffin (EC) cell density and serotonin 5-hydroxytryptamine (5-HT) content were investigated to identify the effect of YPF powder on colonic 5-HT availability. Results: The results showed that the body weight of colitis mice was markedly decreased by 10, 12, 14, and 17% at 1, 3, 5, and 7 days (P < 0.05), whereas stool consistency score (3.6 vs. 0.4, P < 0.05), histopathological score (3.6 vs. 0.3, P < 0.05), and MPO activity (2.7 vs. 0.1, P < 0.05) in colitis mice were significantly increased compared to that of the normal mice; YPF powder treatment dose-dependently increased the body weight (7–13% increase) and decreased the stool consistency score (0.4–1.4 decrease), histopathological score (0.2–0.7 decrease), and MPO activity (0.1–0.9 decrease) in colitis mice. Colonic EC cell density (70% increase) and 5-HT content (40% increase) were markedly increased in colitis mice (P < 0.05), YPF powder treatment dose-dependently reduced EC cell density (20–50% decrease), and 5-HT content (5–27% decrease) in colitis mice. Conclusion: The findings demonstrate that the anti-inflammatory effect of YPF powder on TNBS - induced colitis may be mediated via reducing EC cell hyperplasia and 5-HT content. The important role of YPF powder in regulating colonic EC cell number and 5-HT content may provide an alternative therapy for colonic inflammation.
- Published
- 2015
8. Oridonin Alleviates Visceral Hyperalgesia in a Rat Model of Postinflammatory Irritable Bowel Syndrome: Role of Colonic Enterochromaffin Cell and Serotonin Availability.
- Author
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Kai-hong Zang, Yun-yun Shao, Xiao Zuo, Zhi Rao, and Hong-yan Qin
- Subjects
- *
PROTEIN metabolism , *HYPERALGESIA , *ALTERNATIVE medicine , *ANALGESICS , *ANIMAL experimentation , *COLON (Anatomy) , *DOSE-effect relationship in pharmacology , *HISTOLOGICAL techniques , *INTERFERONS , *INTERLEUKINS , *IRRITABLE colon , *RATS , *SEROTONIN , *SPLEEN , *TUMOR necrosis factors , *PLANT extracts , *CYTOMETRY , *PAIN threshold , *IN vivo studies , *PHARMACODYNAMICS , *PREVENTION - Abstract
The aim of this present study was to investigate the effect of oridonin on visceral hyperalgesia and colonic serotonin availability in a rat model of trinitrobenzenesulfonic acid-induced postinflammatory irritable bowel syndrome (PIIBS). Rats were randomly divided into five groups: normal control, PI-IBS model, PI-IBS+low-dose oridonin (5 mg/kg), PIIBS+ median-dose oridonin (10 mg/kg), and PI-IBS+high-dose oridonin (20 mg/kg). Rats in control and model groups were orally administered with water by gavage, whereas rats in oridonin-treated groups were orally administered with different dosages of oridonin, and drugs were given for 14 consecutive days. Compared with the control group, the pain threshold pressure was significantly reduced in PI-IBS rats. The colonic enterochromaffin (EC) cell number, serotonin content, and the protein expression of tryptophan hydroxylase (TPH) were markedly increased and the protein expression of serotonin reuptake transporter was significantly decreased in PI-IBS rats. The spleen index in PI-IBS rats was decreased, and the levels of tumor necrosis factor (TNF)-α, interferon (IFN)-γ, interleukin (IL)-4, and IL-13 in the colon of PI-IBS rats were also markedly decreased. Oridonin treatment dose dependently increased pain threshold pressure, and markedly decreased colon EC cell numbers, TPH expression, and serotonin content in PI-IBS rats. Oridonin treatment also significantly increased the spleen index as well as the levels of TNF-,α IFN-γ, IL-4, and IL-13 in the colon of PI-IBS rats. Results of this study demonstrate that the analgesic effect of oridonin in PI-IBS rats is associated with reduced colonic EC cell hyperplasia and 5-HT availability, the regulatory effect of oridonin on colonic cytokine production may be correlated with its effect on colonic EC cell number. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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