61 results on '"Kagan O"'
Search Results
2. Impact of preimplantation genetic testing for aneuploidies (PGT-A) on first trimester biochemical markers – PAPP-A (placenta-associated plasma protein) and free b-hCG (human chorionic gonadotropin).
- Author
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Markova, D., Kagan, O., Hoopmann, M., Abele, H., Coughlan, C., Abecia, E., Fatemi, H. M., and Lawrenz, B.
- Subjects
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CHORIONIC gonadotropins , *BLOOD proteins , *BIOMARKERS , *GENETIC testing , *EMBRYO transfer - Abstract
Objective: The objective of the study was to study the effect of preimplantation genetic testing for aneuploidies (PGT-A) performed at blastocyst stage on the levels of first trimester biomarkers. Methods: This is an observational, collaborative, retrospective study. Seven hundred and twenty-eight patients were included in the study. Patients were with singleton pregnancies resulting from either natural conception (NC), or assisted reproductive techniques (ARTs) with PGT-A and frozen embryo transfer (FET) (ART/PGT-A/FET) or after ART without PGT-A and fresh ET (ART/no PGT-A/fresh ET) or FET (ART/no PGT-A/FET), who had first trimester combined screening test between 11 and 14 gestational weeks. They were stratified into four groups: group A (ART/PGT-A/FET) – 143 patients; group B (ART/no PGT-A/FET) – 100 patients; group C (ART/no PGT-A/fresh ET) – 346 patients, and group D (NC) – 139 patients. Results: Statistically significant differences among the examined groups were observed for maternal age, BMI, ethnicity, and parity. The median placenta-associated plasma protein (PAPPA) was lowest in the group with ART/PGT-A/FET and the highest result was obtained in the group with ART/no PGT-A/FET. Statistically significant difference in the median PAPP-A levels was identified among the examined groups (p = .0186). When a subgroup analysis was performed, a statistically significant difference was observed in the median PAPP-A between ART/ PGT-A/FET group versus ART/no PGT-A/FET group (p = .01) and NC versus ART/no PGT-A/FET (p = .01). A similar trend toward statistical significance was noted when comparing NC versus ART/no PGT-A/fresh ET (p = .06). Multivariate analysis elucidated that when age is present in the model, the effect of any method of conception or testing for aneuploidy disappears. The other factors (BMI, ethnicity, and parity) do not influence the levels of PAPP-A. The lowest median free human chorionic gonadotropin (b-HCG) was recorded in the NC group and the highest result was identified in the group with IVF/PGT-A/FET. No statistically significant difference was observed in the median concentration levels of free b-hCG among the compared groups (p = .5789) and when subgroup analysis was performed (p>.05). The normality of the distribution of variables was analyzed by the Kolmogorov–Smirnov test and the median PAPP-A and free bhCG concentration difference by the Wilcoxon rank-sum test with nonparametric ANOVA. Conclusions: Testing for aneuploidy (PGT-A) and the decision to transfer either fresh or cryopreserved embryos (ET) appear not to affect the levels of first trimester biochemical markers. The findings of the present study should be a baseline for future studies and could be used to improve the antenatal screening counseling for women with ART pregnancies and PGT-A. [ABSTRACT FROM AUTHOR]
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- 2022
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3. 5. Viability and Functional Outcomes of Limb Allografts Following Sub-normothermic Plasma and Red Blood Cell Based Perfusion
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Amir Dehdashtian, MD, MPH, Erin Guy, BS, Anna Rieggers, BS, Keith Kozma, BS, Paul S Cederna, MD, Stephen WP Kemp, PhD, and Kagan Ozer, MD
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Surgery ,RD1-811 - Published
- 2024
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4. Impact of preimplantation genetic testing for aneuploidies (PGT-A) on first trimester biochemical markers – PAPP-A (placenta-associated plasma protein) and free β-hCG (human chorionic gonadotropin)
- Author
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Markova, D., primary, Kagan, O., additional, Hoopmann, M., additional, Abele, H., additional, Coughlan, C., additional, Abecia, E., additional, Fatemi, H. M., additional, and Lawrenz, B., additional
- Published
- 2021
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5. Mammakarzinom in der Schwangerschaft
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Oberhoff, C., Kimmig, R., and Kagan, O.
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- 2002
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6. Crystal structure of YIR035C short chain dehydrogenases/reductase from Saccharomyces cerevisiae
- Author
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Stogios, P.J., primary, Skarina, T., additional, Chen, C., additional, Kagan, O., additional, Iakounine, A., additional, and Savchenko, A., additional
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- 2020
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7. Gender discrimination in haematopoietic stem cell transplantation: is it real?: O423
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Rozovski, U., Gepstein, L., Abadi, U., Tavor, S., Gur, O., Ben-Tal, O., Trestman, S., Aviv, F., Kirgner, I., Eshel, R., Rom, E., Kagan, O., and Naparstek, E.
- Published
- 2009
8. Evaluation of Water and Sediment Quality in Lake Mogan, Türkiye
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Kagan Ozdemir, Mirac Nur Ciner, Huseyin Kurtulus Ozcan, and Serdar Aydın
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Mogan Lake ,water quality ,surface sediments ,organic pollution ,Hydraulic engineering ,TC1-978 ,Water supply for domestic and industrial purposes ,TD201-500 - Abstract
The wetlands, with their delicate ecosystems, play a crucial role in regulating water regimes and supporting diverse plant and animal communities, particularly those associated with water habitats. Mogan Lake, located within the Gölbaşı Special Environmental Protection Area, stands out as a unique habitat, hosting over 200 bird species. This study aimed to assess the current water quality of Mogan Lake by analysing various water quality variables. Three sampling sites, representing the northern, middle, and southern parts of the lake, were selected to examine both surface water and bottom sludge characteristics through the analysis of 29 pollutant variables. Water samples were collected from 30 cm beneath the water surface and 50 cm above the bottom of the lake. Sediment samples were collected from the sludge at the lake basin. Additionally, to understand their impact on the lake’s water quality, 26 pollutants were also measured in water samples taken from the five main streams that feed the lake. The results reveal a significant level of organic pollution in the lake, along with elevated nitrogen levels indicating hypertrophic conditions. Although organic pollutants were detected in the lake bottom sediment through analysis, they are considered non-hazardous in terms of heavy metals and other inorganic variables.
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- 2024
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9. Fetal loss after chorionic villus sampling in twin pregnancy.
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Elger, T., Akolekar, R., Syngelaki, A., De Paco Matallana, C., Molina, F. S., Gallardo Arozena, M., Chaveeva, P., Persico, N., Accurti, V., Kagan, K. O., Prodan, N., Cruz, J., Nicolaides, K. H., and Kagan, O
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CHORIONIC villus sampling ,PREGNANCY ,OBSTETRICS ,PREGNANCY tests ,LOGISTIC regression analysis ,TWINS - Abstract
Objective: To estimate the chorionic villus sampling (CVS)-related risk of fetal loss in twin pregnancy after adjustment for chorionicity, nuchal translucency thickness (NT), intertwin discordance in crown-rump length (CRL), maternal demographic characteristics and serum pregnancy-associated plasma protein-A (PAPP-A) and free β-human chorionic gonadotropin (β-hCG).Methods: This was a multicenter study from eight fetal medicine units in which the leadership were trained at the Harris Birthright Research Centre for Fetal Medicine in London, UK, and in which the protocols for screening, invasive testing and pregnancy management are similar. Data were obtained prospectively from women with twin pregnancy undergoing routine ultrasound examination at 11-13 weeks' gestation. Multivariable logistic regression analysis with backward stepwise elimination was used to examine whether CVS provided a significant independent contribution to the prediction of risk of fetal loss after adjusting for maternal and pregnancy characteristics, including maternal age, racial origin and weight, method of conception, smoking status, parity, chorionicity, intertwin discordance in CRL, fetal NT ≥ 95th percentile and free β-hCG and PAPP-A multiples of the median. Similarly, within the CVS group, multivariable logistic regression analysis was used to investigate the effect of the number of intrauterine needle insertions and size of the needle on the risk of fetal loss.Results: The study population of 8581 twin pregnancies undergoing ultrasound examination at 11-13 weeks' gestation included 316 dichorionic and 129 monochorionic twins that had CVS. First, in twin pregnancies undergoing CVS, compared to those not undergoing CVS, there was a 2-fold increased risk of fetal loss at < 24 weeks' gestation and of loss at any stage in pregnancy. Second, the factors providing a significant independent contribution to the prediction of miscarriage or fetal loss in twin pregnancy were increased maternal weight, black racial origin, monochorionicity, and more so monoamnionicity, large intertwin discordance in CRL and increased fetal NT, and, in the case of fetal loss at any stage, there was also a contribution from assisted conception and low serum PAPP-A. Third, after adjustment for maternal and pregnancy characteristics, CVS did not provide a significant contribution to the risk of fetal loss. Fourth, in twin pregnancies that had CVS, there was no significant contribution to fetal loss from the number of intrauterine needle insertions or needle size.Conclusion: The 2-fold increased risk of fetal loss following CVS in twin pregnancy can, to a great extent, be explained by maternal and pregnancy characteristics rather than the invasive procedure itself. © 2021 International Society of Ultrasound in Obstetrics and Gynecology. [ABSTRACT FROM AUTHOR]- Published
- 2021
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10. Fetale Verhaltensstadien und Hirnantworten unter Lichtstimulation, Tonstimulation und in Ruhe – eine fMKG- Studie
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Jacobi, J, additional, Schleger, F, additional, Preissl, H, additional, Mat Husin, H, additional, Pauluschke-Fröhlich, J, additional, Brändle, J, additional, Kagan, O, additional, Brucker, SY, additional, and Kiefer-Schmidt, I, additional
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- 2018
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11. Adjectives in layers
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Pereltsvaig, А., primary and Kagan, O., additional
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- 2018
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12. MANAGEMENT AND LEADERSHIP TYPE IN AUTOMOTIVE INDUSTRY: VIEW FROM TURKEY
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Kağan OKATAN
- Subjects
management ,leadership ,organizational culture ,production management ,technology and innovation management ,yönetim ,liderlik ,örgütsel kültür ,üretim yönetimi ,Social sciences (General) ,H1-99 - Abstract
ABSTRACT: Management' and 'leadership' culture in automotive industry which has an important place in Turkey’s exports, was measured with examples. Organizational culture was measured according to the typologies of 'market', ‘adhocracy', 'clan' and ‘hierarchy'. 'Competing values framework’ was used as a measurement tool on the collected first hand data. The use of the competing values framework was also enabled the identification of general organizational culture in exemplary corporations. As a result of the study, it was demonstrated that dominance of 'market' type culture which is customer and external oriented and also focuses on success and competition, in the management and leadership at automotive industry. The market culture type has been observed as the top culture type, especially in the 'organizational leadership’ dimensions of all sample corporations. In light of these results, the study showed us that the perception of success in management and leadership is intensely focused on 'business outcomes'. ÖZ: Bu çalışmada Türkiye ihracatında önemli bir yere sahip olan otomotiv sektöründe 'yönetim' ve 'liderlik' kültürü örneklerle ölçülmüştür. Örgütsel kültür, 'pazar', 'adhokrasi', 'klan' ve 'hiyerarşi' tipolojilerine göre ölçülmüştür. İlk elden toplanan veriler üzerinde bir ölçüm aracı olarak 'rekabetçi değerler çerçevesi' kullanılmıştır. ‘Rekabetçi değerler çerçevesinin’ kullanılması, örnek şirketlerde genel organizasyon kültürünün tanımlanmasını da sağlamıştır. Çalışma sonucunda otomotiv endüstrisinin yönetiminde ve liderliğinde, müşteri ve dış odaklı, aynı zamanda başarı ve rekabete odaklanan 'pazar' tipi kültürün hakimiyeti ortaya konmuştur. Pazar kültürü türü, özellikle tüm örnek şirketlerin organizasyonel liderlik' boyutlarında en üst kültür türü olarak gözlemlenmiştir. Bu sonuçlar ışığında çalışma bize, yönetimde ve liderlikte ‘başarı’ algısının yoğun bir şekilde 'iş sonuçlarına' odaklandığını göstermiştir.
- Published
- 2021
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13. P182-M Reproducibility of the Protein Forest digital Proteome Chip as a Protein Isoelectric Fractionation Tool
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Sin, A., Dasch, J., Kagan, O., Kandola, P., Artwell-Doucette, D., Haralampu, S., Skea, W., and Garlick, R.
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Poster Abstracts: Proteomics - Abstract
Protein fractionation is often necessary in mass spectrometry (MS) proteomic studies to help detect low-abundance proteins. Protein Forest has developed the digital ProteomeChip (dPC) technology, which is a rapid, reproducible, and easy to use protein MS pre-fractionation tool.
- Published
- 2007
14. Maternale Nifedipin-Gabe beeinflusst das fetale Lungenwachstum im Nitrofen-Rattenmodell für die kongenitale Zwerchfellhernie nicht
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Selinsek, L, primary, Hauer, J, additional, Abele, H, additional, Kagan, O, additional, Deprest, J, additional, Hilcher, U, additional, Iglauer, F, additional, Haen, S, additional, Wallwiener, D, additional, and Beck, V, additional
- Published
- 2013
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15. Assessment of the health workers knowledge and belief about rheumatic and musculoskeletal diseases and spa treatments: A descriptive study
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Erdal Dilekci, Kagan Ozkuk, and Baris Kaki
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rheumatic and musculoskeletal diseases ,spa treatments ,health facility workers ,knowledge level ,health training ,Medicine - Abstract
To investigate identifying the knowledge levels, behavior attitude and belief levels about rheumatic and musculoskeletal diseases (RMDs) and spa treatments (ST) among health workers in communication with patients and determining target groups for health training. Cross-sectional, descriptive study. Bolu Izzet Baysal Physical Medicine and Rehabilitation Education and Research Hospital and Izzet Baysal Education and Research Hospital 01.04.2019-15.04.2019. 600 participants completed 6 descriptive questions with yes-no answers about demographic characteristics and completed a survey determining knowledge levels about RMDs and ST as correct or incorrect. Though there was statistically significant difference in favor of women about RMDs knowledge level, there was no statistically significant difference between 2 groups for ST knowledge levels. When RMDs and ST knowledge levels are compared, doctors and physiotherapists had statistically similar knowledge levels, with statistically significant level of difference compared to the other groups. Among groups reporting similar opinions about the efficacy of ST, there was no statistically significant difference identified between RMDs knowledge levels, with a statistically significant level of difference identified for ST knowledge levels. Apart from doctors and physiotherapists, 307 other health workers did not have RMDs themselves or in their family, while only 167 had RMDs themselves and 126 stated both they and their family had RMDs. There was a statistically significant difference identified for RMDs knowledge levels. We found that even among the health workers who communicate with patients in hospitals, the level of knowledge with RMDs and ST is very variable. Taking this into account, we believe that all personnel should be provided with supportive scientific information and training in order to ensure that the health workers who are intertwined with the health problems of the individuals reach the correct information. [Med-Science 2019; 8(4.000): 901-7]
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- 2019
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16. Bromide soaked structure of an esterase from the oil-degrading bacterium Oleispira antarctica
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Petit, P., primary, Dong, A., additional, Kagan, O., additional, Savchenko, A., additional, and Yakunin, A.F., additional
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- 2011
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17. Crystal structure of ALR0039, a putative alpha/beta hydrolase from Nostoc sp PCC 7120.
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Petit, P., primary, Kagan, O., additional, Yakunin, A.F., additional, and Savchenko, A., additional
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- 2011
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18. Crystal structure of the ligand binding domain of E. coli CynR with its specific effector azide
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Singer, A.U., primary, Evdokimova, E., additional, Kagan, O., additional, Dong, A., additional, Edwards, A.M., additional, and Savchenko, A., additional
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- 2009
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19. 93. The Effects of Subnormothermic Plasma and Red Blood Cell Based Perfusion on the Function and Viability of Limb Allografts
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Amir Dehdashtian, MD, MPH, Erin L. Guy, BS, Anna E. Riegger, BS, Keith D. Kozma, BS, Paul S. Cederna, MD, Stephen WP Kemp, PhD, and Kagan Ozer, MD
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Surgery ,RD1-811 - Published
- 2022
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20. Mammotome vs. ATEC, Vergleich von 2 sonografisch kontrollierten Vakuumbiopsiesystemen für die Brust
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Hahn, M, primary, Fischbach, E, additional, Kagan, O, additional, Fehm, T, additional, Krainick-Strobel, U, additional, Wallwiener, D, additional, and Gruber, I, additional
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- 2008
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21. 2 Vakuumbiopsiesysteme der Brust im Vergleich: Mammotome vs. ATEC
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Hahn, M, primary, Fischbach, E, additional, Kagan, O, additional, Fehm, T, additional, Wallwiener, D, additional, and Gruber, I, additional
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- 2008
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22. Osseoscopy: Direct Visualization to Assist Core Decompression and Debridement of Necrotic Bone Defects
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William C. Geisert, M.D., Aaron M. Perdue, M.D., and Kagan Ozer, M.D.
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Orthopedic surgery ,RD701-811 - Abstract
Free vascularized fibular grafting after core decompression and debridement of necrotic lesions is an effective surgical treatment of avascular necrosis of the femoral head. A technical challenge encountered in performing this procedure is ensuring adequate debridement of necrotic parts while preserving healthy bone. A previously described method accomplishes this indirectly using radioactive contrast media and fluoroscopy, increasing the risk of radiation exposure. We propose a surgical technique using standard arthroscopic equipment to visualize inside the femoral head, facilitating precise and accurate debridement without additional radiation exposure.
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- 2017
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23. Sonographic Criteria for the Differentiation of Benign and Malignant Breast Lesions using Real-Time Spatial Compound Imaging in Combination with XRES Adaptive Image Processing
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Roessner, Lisa Charlotte and Kagan, O. (PD Dr.)
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Ultraschalldiagnostik ,Real-Time Compound-Imaging , XRES Adaptive Image Processing , Sonography , Criteria of Dignity ,Mammasonographie , Dignitätskriterien - Abstract
Insgesamt 460 Patienten im Alter von durchschnittlich 50,9 Jahren (Range: 17,5- 91,3 Jahre) wurden in diese retrospektive Studie eingeschlossen. Alle Patienten erhielten eine Sonographie unter Anwendung der Bildoptimierungsverfahren des „SonoCT Real-Time Compound Imaging“ und „XRES Adaptive Image Processing“. Zur Verwendung kamen die Ultraschallgeräte iU 22 und HD11 von der Firma Philips (Hamburg). Alle Befunde wurden durch 7 erfahrene Untersucher im Ultraschall hinsichtlich ihrer Dignitätskriterien untersucht und nach BI-RADS klassifiziert. Die Dignitätskriterien umfassten Form, Achse, Rand, Echogenität und Schallfortleitung. Die Verteilung der BI-RADS-Kategorisierung wurde erfasst und hinsichtlich des positiv prädiktiven Wertes (ppV), des negativ prädiktiven Wertes (npV), der Sensitivität und der FPR beurteilt. Die einzelnen Dignitätskriterien wurden im Rahmen einer Regressionsanalyse bezüglich ihrer Wertigkeit überprüft und miteinander verglichen. Bei allen Patienten lag zum Zeitpunkt der Aufnahme in die Studie das pathologische Ergebnis der Gewebeprobe vor. Zunächst wurde die Häufigkeitsverteilung der histologischen Ergebnisse ermittelt. Insgesamt handelt es sich hierbei um 269 benigne und 191 maligne Befunde. Unter den benignen Befunden war die fibrös-zystische Mastopathie bzw. einfach-fibröse Mastopathie mit 93/269 (35%) am häufigsten, sowie das Fibroadenom mit 90/269 (34%) am zweithäufigsten zu finden. Bei den malignen Befunden steht das invasiv duktale Carcinom mit 130/191 (68%) in Bezug auf die Häufigkeit an erster und das invasiv lobuläre Carcinom mit 36/191 (19%) an zweiter Stelle. Die Beurteilung der Befunde nach BI-RADS ergab einen Vorhersagewert für Malignität von 65,1%, einen negativen Vorhersagewert von 95,5% sowie eine Sensitivität von 95,8% und eine FPR von 36,5%. In der Kategorie BI-RADS 2, also gutartig, war ein maligner Befund zu finden. Bei diesem Befund handelte es sich um ein alveoläres Weichteilsarkom, also nicht um einen typischen Herdbefund in der Brust. Daraus ergibt sich der positive Vorhersagewert für BI-RADS 2 Befunde von 4,5%. Der PPV für die Kategorie BI-RADS 3 liegt ebenfalls bei 4,5% und entspricht somit den Referenzwerten in der Literatur. Ein als BI-RADS 4 eingestufter Befund hingegen ergab in 39% der Fälle eine maligne Histologie während dieser Wert bei den BI-RADS 5 Befunden bereits bei 93,3% liegt. Insgesamt kann gesagt werden, dass die BI-RADS-Klassifikation eine standardisierte Methode zur Befundinterpretation und für das weitere Vorgehen liefert. Zudem erleichtert die Anwendung von BI-RADS die interkollegiale Kommunikation und führt zu einer besseren Vergleichbarkeit. Alle Dignitätskriterien erreichten im Rahmen der deskriptiven Auswertung (Chi Square) das Signifikanzniveau. Hinsichtlich der Wertigkeit der Dignitätskriterien ergab die multivariate Regressionsanalyse vier signifikante Kriterien zur Prädiktion maligner Befunde. Dabei handelt es sich um die polymorphe Form (OR: 5,517), den gelappten Rand (OR: 3,530), den unscharfen Rand (OR: 2,509) und die abgeschwächte Schallfortleitung (OR: 2,383). In der univariaten Analyse erreichte auch die indifferente und vertikale Achse das Signifikanzniveau. Durch Anwendung der in der multivariaten Regression signifikanten Kriterien ergibt sich ein deutlicher Anstieg der Detektionsrate bei gleichzeitig geringerer Falsch-Positiv-Rate. Um eine Detektionsrate von >95% zu erzielen, muss mit einer Falsch-Positiv-Rate von 60% gerechnet werden. Dabei zeigt sich eine deutliche Diskrepanz des Regressionsmodells zu den im klinischen Alltag ermittelten BI-RADS-Klassifikationen der Befunde. Hier beträgt die Falsch-Positiv-Rate bei einer Sensitivität von 95,8% lediglich 36,5%. Dies ist sowohl auf die Kenntnisse von Vorbefunden (Mammographie, MRT, klinische Untersuchung) zum Untersuchungszeitpunkt als auch auf die Erfahrung des Untersuchers zurückzuführen. Bei Betrachtung der Ergebnisse der vorliegenden Arbeit wird deutlich, dass durch die Anwendung von zusätzlichen Bildverbesserungstechniken wie das Compound Imaging in Kombination mit Adaptive Image Processing der Herdbefund an sich besser beurteilt werden kann. Durch die verbesserte Auflösung werden vor allem die Kriterien Form und Rand wichtig. Bildartefakte und Nebenerscheinungen wie beispielsweise das dorsale Schallverhalten spielen eine untergeordnete Rolle. PURPOSE: The purpose of the study was to analyze sonographic criteria for the differentiation of benign and malignant breast lesions using real-time spatial compound imaging (CT) in combination with adaptive image processing (XRES). MATERIALS AND METHODS: In a retrospective analysis of 460 patients, the sonographic criteria: shape, orientation, margin, echo pattern and posterior acoustic features were determined using CT and XRES. All investigations were performed using a 12 MHz linear transducer. The findings were classified according to the DEGUM criteria analogous to BIRADS and were histologically confirmed by core needle or vacuum biopsy. Statistical analysis was performed using a Chi-square test, logistic uni- and multivariate regression analysis and an ROC-curve analysis to detect the false-positive rate. RESULTS: All investigated diagnostic criteria were significant in the descriptive analysis (Chi-squared). The multivariate analysis showed that the criteria of irregular shape versus round, and not circumscribed margin versus circumscribed margin as well as the posterior acoustic features of enhanced versus reduced have a significant influence on the prediction of a malignant finding. The univariate analysis also showed a statistical significance using the indifferent and not parallel orientation versus parallel. To attain a detection rate of > 95 %, a false-positive rate of 60 % must be expected. CONCLUSION: This study shows that when using CT in combination with XRES, the analyzed sonographic criteria for the differentiation of benign and malignant breast lesions are still of diagnostic value. In particular, the shape, margin and posterior sonographic features are important.
- Published
- 2011
24. Implications of artificial intelligence for nurse managers.
- Author
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Ross A, Freeman R, McGrow K, and Kagan O
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- Humans, Artificial Intelligence, Nurse Administrators psychology
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- 2024
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25. Bile Duct Injuries During Urgent Cholecystectomy at a Safety Net Teaching Hospital: Attending Experience and Time of Day May Matter.
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Ugarte C, Zielsdorf S, Ugarte R, Kagan O, Murphy R, Martin MJ, Inaba K, and Schellenberg M
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- Humans, Male, Female, Retrospective Studies, Middle Aged, Aged, Intraoperative Complications epidemiology, Intraoperative Complications etiology, Adult, Safety-net Providers, Risk Factors, Time Factors, Incidence, Bile Ducts injuries, Cholecystitis, Acute surgery, Cholecystectomy adverse effects, Hospitals, Teaching
- Abstract
Background: Bile duct injury (BDI) is one of the most severe complications during cholecystectomy. Early identification of risk factors for BDI may permit risk reduction strategies and inform patient consent. Objective: This study aimed to define patient, provider, and systemic factors associated with BDI; BDI incidence; and short-term outcomes of BDI after urgent cholecystectomy. Methods: Patients who underwent urgent cholecystectomy for acute cholecystitis were retrospectively screened (2020-2022). All patients who sustained BDI were included without exclusions. Demographics, clinical data, and outcomes were collected and compared with descriptive statistics. Results: During the study period, BDI occurred in 4 (0.5%) of 728 patients who underwent urgent cholecystectomy for acute cholecystitis. Most BDI cases (75%) took place overnight or during the weekend. The attending surgeon was almost exclusively (75%) in their first year of practice. BDI was recognized during index operation in 2 cases (50%). Hepatobiliary surgery performed the bile duct repair in all 4 cases. Two complications occurred (50%). All patients were followed by hepatobiliary surgery in the outpatient setting and returned to their baseline level of function within 2 months of hospital discharge. Conclusion: Most BDI occurred in procedures attended by first-year faculty during after hours cholecystectomies, suggesting a role for increased proctorship in early career attendings in addition to in-hours cholecystectomy for acute cholecystitis. The timely return to baseline function experienced by these patients emphasizes the favorable outcomes associated with early recognition of BDI and involvement of hepatobiliary surgery. Further examination with multicenter evaluation would be beneficial to validate these study findings., Competing Interests: Declaration of Conflicting InterestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
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- 2024
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26. Nurses' Evolving Role in Informatics During the Digital Transformation Era.
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Kagan O, Connor M, and McGrow K
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- Humans, Informatics, Nurses
- Published
- 2024
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27. Obstructive sleep apnea as a presentation of congenital central hypoventilation syndrome.
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Kagan O, Zhang C, McElyea C, Keens TG, Davidson Ward SL, and Perez IA
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- Humans, Male, Female, Polysomnography, Infant, Homeodomain Proteins genetics, Transcription Factors, Sleep Apnea, Central genetics, Sleep Apnea, Central diagnosis, Sleep Apnea, Central complications, Sleep Apnea, Central physiopathology, Hypoventilation congenital, Hypoventilation genetics, Hypoventilation complications, Hypoventilation diagnosis, Sleep Apnea, Obstructive diagnosis, Sleep Apnea, Obstructive genetics
- Abstract
Congenital central hypoventilation syndrome is a rare disorder due to a mutation in the PHOX2B gene, characterized by a failure in autonomic control of breathing with diminished or absent response to hypoxia and hypercapnia, which is most pronounced during sleep. Most patients present from birth with central apneas and hypoventilation, or later in the setting of a physiologic stress. Recent literature in mice with a Phox2b27Ala/+ mutation suggests a predisposition to obstructive apneas likely due to hypoglossal dysgenesis. We report on three patients with obstructive sleep apneas with absent or mild hypoventilation. Our cases propose that obstructive apneas can be the primary presentation in patients who subsequently develop the classic phenotype of congenital central hypoventilation syndrome and emphasize their close monitoring and surveillance., Citation: Kagan O, Zhang C, McElyea C, Keens TG, Davidson Ward SL, Perez IA. Obstructive sleep apnea as a presentation of congenital central hypoventilation syndrome. J Clin Sleep Med . 2023;19(9):1697-1700., (© 2023 American Academy of Sleep Medicine.)
- Published
- 2023
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28. The Impact of Leadership on the Continued Growth of the Nursing, Informatics Community in New York State and Beyond.
- Author
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Kagan O, Connor M, and Serwicki V
- Subjects
- Humans, New York, Leadership
- Published
- 2023
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29. Nurses' confidence in starting a new venture, startup or project in the context of nurse-led hackathons: Results of prehackathon survey.
- Author
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Kagan O, Sciasci NG, Koszalinski RS, Kagan DH, Leary M, and Nadel H
- Subjects
- Humans, Male, Nurse's Role, Retrospective Studies, COVID-19 epidemiology, Nurses
- Abstract
Background: A hackathon framework has been successfully applied to solving health care challenges, including COVID-19, without much documented evidence of nurses' baseline or acquired confidence., Purpose: To understand differences in baseline confidence levels in starting a new venture, startup or project in the context of nurse-led hackathons., Method: A retrospective secondary analysis of a presurvey of hackathon participants from two NurseHack4Health (NH4H) events held in 2021., Discussion: Male nurses and international nurses were more confident than the U.S.-based nurses. When comparing the 75% of participants who had not attended a hackathon previously to the 25% of participants who had, there was an increased confidence level among non-nurses and among participants with the previous hackathon, datathon, and ideation experience., Conclusion: If hackathons can help nurses identify strengths, add new expertise and boost confidence, it may empower nurses to pursue their ideas more effectively, aid professional growth, and provide affirmation of innovator self-identity., (Copyright © 2023 Elsevier Inc. All rights reserved.)
- Published
- 2023
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30. Leveraging Comprehensive Health Records for Breast Cancer Risk Prediction: A Binational Assessment.
- Author
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Chorev M, Barros V, Spiro A, Evron E, Barkan E, Kagan O, Amit M, Ozery-Flato M, Akselrod-Balin A, Shalev V, Rosen-Zvi M, and Guindy M
- Subjects
- Humans, Female, Mammography, Retrospective Studies, Early Detection of Cancer, Breast, Risk Assessment, Breast Neoplasms
- Abstract
Breast cancer (BC) risk models based on electronic health records (EHR) can assist physicians in estimating the probability of an individual with certain risk factors to develop BC in the future. In this retrospective study, we used clinical data combined with machine learning tools to assess the utility of a personalized BC risk model on 13,786 Israeli and 1,695 American women who underwent screening mammography in the years 2012-2018 and 2008-2018, respectively. Clinical features were extracted from EHR, personal questionnaires, and past radiologists' reports. Using a set of 1,547 features, the predictive ability for BC within 12 months was measured in both datasets and in sub-cohorts of interest. Our results highlight the improved performance of our model over previous established BC risk models, their ultimate potential for risk-based screening policies on first time patients and novel clinically relevant risk factors that can compensate for the absence of imaging history information., (©2022 AMIA - All rights reserved.)
- Published
- 2023
31. Successful on-ECLS Repair of CDH and Omphalocele in a Newborn.
- Author
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Fideler F, Mustafi M, Kirschner HJ, Gerbig I, Fuchs J, Hofbeck M, Kumpf M, Kagan O, Michel J, Jost W, and Neunhoeffer F
- Abstract
Both congenital diaphragmatic hernias (CDHs) and omphaloceles show relevant overall mortality rates as individual findings. The combination of the two has been described only sparsely in the literature and almost always with a fatal course. Here, we describe a term neonate with a rare high-risk constellation of left-sided CDH and a large omphalocele who was successfully treated on extracorporeal life support (ECLS). Prenatally, the patient was diagnosed with a large omphalocele and a left CDH with a lung volume of ∼27% and an observed to expected lung-to-head ratio of 30%. Due to respiratory insufficiency, an ECLS device was implanted. As weaning from ECLS was not foreseeable, the female infant underwent successful surgery on ECLS on the ninth day of life. Perioperative high-frequency oscillatory ventilation and circulatory and coagulation management under point-of-care monitoring were the main anesthesiological challenges. Over the following 3 days, ECLS weaning was successful, and the patient was extubated after another 43 days. Surgical treatment on ECLS can expand the spectrum of therapy in high-risk constellations if potential risks are minimized and there is close interdisciplinary cooperation., Competing Interests: Conflict of Interest None declared, (The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. ( https://creativecommons.org/licenses/by/4.0/ ).)
- Published
- 2023
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32. Multifaceted role of the registered nurse on an oral immunotherapy clinical team.
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Russell AF, Cooke AJT, Kagan O, and Stieb ES
- Abstract
Oral immunotherapy (OIT) emerged into clinical practice, and its delivery highlights the multifaceted expertise of registered nurses (RN) as central to allergy/immunology interprofessional collaborative teams. The allergist-RN model of clinical evidenced-based OIT provision is presented. RN competencies, role components, and intervention examples are included to assist RNs and allergists in maximizing RN capabilities. RNs' patient-centered focus, and the ability to evaluate and incorporate physical, psychological, and sociological patient aspects are assets to OIT teams. RNs can establish best practices, initiate scholarly inquiry, and disseminate new knowledge to interdisciplinary colleagues. RNs also implement allergist-prescribed standing protocols within their legal practice scope by using their clinical judgment during evaluation of a patient receiving OIT. The same RN may serve as a nurse clinician, patient and family educator, case manager, research collaborator, and OIT program manager. Allergy/immunology practices use diverse staffing models, which thus require adaptation of presented descriptions per clinical team needs and resources., Competing Interests: A.F. Russell serves on the editorial board of Journal of Food Allergy; E.S. Stieb discloses royalties from McGraw Hill Education, Inc. for co-authorship of the Asthma Educator's Handbook. The remaining authors have no conflicts of interest to disclose pertaining to this article, (Copyright © 2022, The Author(s). Published by OceanSide Publications, Inc., U.S.A.)
- Published
- 2022
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33. AI-assisted tracking of worldwide non-pharmaceutical interventions for COVID-19.
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Suryanarayanan P, Tsou CH, Poddar A, Mahajan D, Dandala B, Madan P, Agrawal A, Wachira C, Samuel OM, Bar-Shira O, Kipchirchir C, Okwako S, Ogallo W, Otieno F, Nyota T, Matu F, Barros VR, Shats D, Kagan O, Remy S, Bent O, Guhan P, Mahatma S, Walcott-Bryant A, Pathak D, and Rosen-Zvi M
- Subjects
- Global Health, Humans, Artificial Intelligence, COVID-19 prevention & control, COVID-19 therapy, Communicable Disease Control trends
- Abstract
The Coronavirus disease 2019 (COVID-19) global pandemic has transformed almost every facet of human society throughout the world. Against an emerging, highly transmissible disease, governments worldwide have implemented non-pharmaceutical interventions (NPIs) to slow the spread of the virus. Examples of such interventions include community actions, such as school closures or restrictions on mass gatherings, individual actions including mask wearing and self-quarantine, and environmental actions such as cleaning public facilities. We present the Worldwide Non-pharmaceutical Interventions Tracker for COVID-19 (WNTRAC), a comprehensive dataset consisting of over 6,000 NPIs implemented worldwide since the start of the pandemic. WNTRAC covers NPIs implemented across 261 countries and territories, and classifies NPIs into a taxonomy of 16 NPI types. NPIs are automatically extracted daily from Wikipedia articles using natural language processing techniques and then manually validated to ensure accuracy and veracity. We hope that the dataset will prove valuable for policymakers, public health leaders, and researchers in modeling and analysis efforts to control the spread of COVID-19.
- Published
- 2021
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34. Factors associated with college students' willingness and readiness to act in a food allergic emergency (WilRAFAE).
- Author
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Kagan O
- Subjects
- Adolescent, Adult, Age Factors, Epinephrine administration & dosage, Female, Humans, Male, Middle Aged, Socioeconomic Factors, Universities, Young Adult, Anaphylaxis drug therapy, Epinephrine therapeutic use, Food Hypersensitivity drug therapy, Students psychology
- Abstract
Objective: Food allergies are on the rise in the U.S. Factors associated with willingness and readiness to act in a food allergic emergency on a college campus are currently unknown. Participants: College students in one Catholic college enrolled during spring of 2017. Methods: A previously piloted survey was distributed by e-mail. Results: Four hundred seventy-four individuals responded. All readiness components correlated, and all willingness components correlated with each other. Age, having children, college major had statistically significant correlations with readiness and willingness to act. Readiness was highly predictive of willingness to act in an FAE. Thirty-five percent of variability in willingness to act was explained by age, being health professions students, desire to be trained, social desirability, and readiness. Students in nonhealth related majors expressed high willingness, but low readiness. Conclusion: A pool of willing, trained to act individuals should be considered on college campuses including availability of unassigned epinephrine auto-injector.
- Published
- 2020
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35. [Efficiency and safety of phenazopyridine for treatment of uncomplicated urinary tract infection: results of multi-center, randomized, placebo-controlled, clinical study].
- Author
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Petrov SB, Slesarevskaya MN, Chibirov KH, Topuzov ME, Kagan OF, and Voronova EN
- Subjects
- Adult, Anti-Bacterial Agents, Double-Blind Method, Female, Humans, Phenazopyridine, Treatment Outcome, Cystitis, Urinary Tract Infections
- Abstract
Aim: to evaluate the efficiency and safety of phenazopyridine for the treatment of patients with uncomplicated lower urinary tract infection, accompanied by pain., Materials and Methods: A multicenter double-blind, randomized, placebo-controlled study with parallel groups to evaluate the efficacy and safety of phenazopyridine in patients with acute uncomplicated cystitis was performed. A total of 60 women were divided into two groups of 30 patients. In the main group (average age 32.6+/-7.4 years) phenazopyridine was prescribed (2 tablets of 100 mg p.o., with a total dose of 200 mg, once). In the control group, patients (mean age 35.53+/-8.79 years) received a placebo according to the same scheme. To evaluate the efficiency of treatment, the severity of the main symptoms 6 hours after taking the drug was analyzed. After that, patients started antibiotic therapy. They were followed-up for the next three days. The tolerance of therapy was evaluated by the presence of adverse events., Results: All 30 patients taking phenazopyridine had an improvement after 6 hours, and the most frequent response was "significant improvement" (43.3%). The responses of patients in the main group significantly (p<0.05) differed from responses of patients in the control group. Six hours after taking phenazopyridine/placebo, the severity of all values according to VAS score, including the degree of general discomfort, pain during urination and increased frequency of urination improved significantly in the main group compared to the control group. The average assessment of general discomfort in the main group decreased by 53.4% in comparison with 28.8% in the control group, while the severity of pain during urination and urination frequency decreased by 57.4 vs. 35.9% and 39.6 vs. 27.6%, respectively. An analysis of the time before the complete absence of the general discomfort was performed. In the main group this period of time was significantly less than in the control group (p<0.05). There were no serious adverse events while taking phenazopyridine. Rate of adverse events was comparable between two groups., Conclusion: The results of the study showed that phenazopyridine is an effective and well-tolerated drug for symptomatic therapy in patients with acute uncomplicated cystitis and can be recommended in addition to etiological therapy.
- Published
- 2020
36. [Imidafenacin for treatment of overactive bladder and urgent urinary incontinence: the results of open-label multicenter randomized controlled clinical trial].
- Author
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Al-Shukri SH, Pushkar DY, Apolikhin OI, Evdokimov MS, Kogan MI, Krivoborodov GG, Kagan OF, Petrov SB, Poltoratskyi AN, Kuzmin IV, and Sharvadze GG
- Subjects
- Adolescent, Adult, Aged, Benzhydryl Compounds, Double-Blind Method, Female, Humans, Male, Middle Aged, Muscarinic Antagonists, Quality of Life, Russia, Tolterodine Tartrate, Treatment Outcome, Young Adult, Imidazoles therapeutic use, Urinary Bladder, Overactive drug therapy, Urinary Incontinence drug therapy
- Abstract
Introduction: The analysis of the results of a multicenter, open, randomized comparative phase III clinical trial on the use of imidafenacin for treating patients with OAB was carried out. A clinical study was conducted according to GCP standards in 12 urological centers of the Russian Federation with the support of company AO "R-Pharm"., Materials and Methods: A total of 296 patients (men and women) aged from 18 to 65 years with OAB and urgent urinary incontinence were included in the study. All patients were randomized into two groups. In Group 1 (n=148) patients received -cholinoblocker imidafenacin 1 tablet (0,1 mg) twice a day. Group 2 patients (n=148) were prescribed a comparison drug tolterodine 1 tablet (2 mg) twice a day, as well. The duration of treatment was 12 weeks., Results: The analysis of results showed a significant decrease in the OAB symptoms in both groups. In Group 1 a decrease of episodes of urge urinary incontinence was more pronounce compared to Group 2, as well as amount of day-time and night-time of episodes of urge urinary incontinence by the 2nd, 4th, 8th and 12th weeks of treatment in comparison with baseline scores. There were no differences between two groups in the severity of reducing average urinary frequency per day. Reducing the severity of urinary disturbances in patients of both groups was accompanied by an improvement in the quality of life. There was a significant and similar decrease in the average total score of both OAB Awareness Tool and EQ-5D questionnaires. Tolerability of treatment was satisfactory in both groups and there were no differences in the adverse events in Group 1 and 2., Conclusion: Imidafenacin showed high clinical efficacy for treating patients with OAB, which is not inferior, and in some values, is superior in comparison to tolterodine. Both drugs had a similar safety and tolerability profile.
- Published
- 2018
37. Parent perspectives on school food allergy policy.
- Author
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Mustafa SS, Russell AF, Kagan O, Kao LM, Houdek DV, Smith BM, Wang J, and Gupta RS
- Subjects
- Adolescent, Child, Epinephrine supply & distribution, Female, Food Labeling, Health Education, Humans, Male, Students, Sympathomimetics supply & distribution, Attitude, Food Hypersensitivity, Nutrition Policy, Parents psychology, Schools organization & administration
- Abstract
Background: Food allergy affects up to 8% of children in the U.S. There is minimal research to date on food allergy policies that are currently in place in schools and the opinions of parents of children with food allergy on the effectiveness of or need for these policies., Methods: An electronic survey was disseminated to parents of children with food allergy. Frequencies were calculated to describe respondent characteristics and responses. Chi-square tests were performed to examine associations between school and child characteristics and outcomes., Results: Of the 289 parent respondents, 27.4% were unsure or felt school was unsafe for their child with food allergy. While the majority felt that the polices in their child's school were helpful, most also believed that implementation of additional polices was necessary, including availability of stock epinephrine (94.2%), lunch menus with allergen information (86%), ingredient labels on food items (81%), and direct food allergy education for students (86%). There were significant differences in school food allergy policy depending on the age of the student body, private versus public school, and geographic location., Conclusions: While most schools reportedly have one or more food allergy policies in place, many parents have concerns over the safety of their child at school and feel that additional policies are necessary to improve the safety of the school environment for children with food allergy. The availability of stock epinephrine, improved allergen labeling of food and menus and increased food allergy education may be key policy areas on which to focus.
- Published
- 2018
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- View/download PDF
38. School nurse perspectives on school policies for food allergy and anaphylaxis.
- Author
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Kao LM, Wang J, Kagan O, Russell A, Mustafa SS, Houdek D, Smith B, and Gupta R
- Subjects
- Humans, Perception, Anaphylaxis, Food Hypersensitivity, Health Policy, Nurses psychology, School Health Services
- Abstract
Background: Although school health care professionals are integral to the management of students with food allergy, their views on school food allergy policies have not yet been reported., Objective: To characterize food allergy policies currently being used in schools and their utility and potential barriers to implementation from the perspective of school health care professionals., Methods: An electronic survey was disseminated to school nurses at the 2016 National Association of School Nurses meeting and through the Allergy and Asthma Network listserv. Frequencies were calculated to describe participant characteristics and responses. Unadjusted associations were examined using χ
2 tests; adjusted associations were examined using multiple logistic regression models., Results: A total of 242 completed surveys were included in the analysis. Thirty-two percent of nurses reported an allergic reaction in their school in the past year. Most schools used a variety of policies, including anaphylaxis training for staff (96.7%), stock epinephrine availability (81.7%), designated lunch areas (62.2%), and food guidelines for classrooms (61.8%). Barriers to implementation included financial, time, and attitudinal considerations. Schools with pre-K or kindergarten students had higher odds of having designated lunch areas (adjusted odds ratio [OR], 2.1; 95% confidence interval [CI], 1.0-4.1; P < .05). The odds of having emergency epinephrine available were higher in schools with a full-time nurse (OR, 2.6; 95% CI, 1.1-6.3; P < .05) and in schools reporting at least 1 severe reaction in the past year (OR, 3.2; 95% CI, 1.2-8.5; P < .05)., Conclusion: With one-third of school nurses reporting an allergic reaction in the past year, schools use many strategies to minimize allergen exposures and increase anaphylaxis preparedness. Most school nurses favor these policies and acknowledge barriers to implementation., (Copyright © 2017 American College of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.)- Published
- 2018
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39. Global landscape of cell envelope protein complexes in Escherichia coli.
- Author
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Babu M, Bundalovic-Torma C, Calmettes C, Phanse S, Zhang Q, Jiang Y, Minic Z, Kim S, Mehla J, Gagarinova A, Rodionova I, Kumar A, Guo H, Kagan O, Pogoutse O, Aoki H, Deineko V, Caufield JH, Holtzapple E, Zhang Z, Vastermark A, Pandya Y, Lai CC, El Bakkouri M, Hooda Y, Shah M, Burnside D, Hooshyar M, Vlasblom J, Rajagopala SV, Golshani A, Wuchty S, F Greenblatt J, Saier M, Uetz P, F Moraes T, Parkinson J, and Emili A
- Subjects
- Cell Membrane chemistry, Membrane Proteins chemistry, Membrane Proteins classification, Membrane Proteins genetics, Multiprotein Complexes chemistry, Multiprotein Complexes classification, Cell Membrane genetics, Escherichia coli genetics, Multiprotein Complexes genetics, Proteomics
- Abstract
Bacterial cell envelope protein (CEP) complexes mediate a range of processes, including membrane assembly, antibiotic resistance and metabolic coordination. However, only limited characterization of relevant macromolecules has been reported to date. Here we present a proteomic survey of 1,347 CEPs encompassing 90% inner- and outer-membrane and periplasmic proteins of Escherichia coli. After extraction with non-denaturing detergents, we affinity-purified 785 endogenously tagged CEPs and identified stably associated polypeptides by precision mass spectrometry. The resulting high-quality physical interaction network, comprising 77% of targeted CEPs, revealed many previously uncharacterized heteromeric complexes. We found that the secretion of autotransporters requires translocation and the assembly module TamB to nucleate proper folding from periplasm to cell surface through a cooperative mechanism involving the β-barrel assembly machinery. We also establish that an ABC transporter of unknown function, YadH, together with the Mla system preserves outer membrane lipid asymmetry. This E. coli CEP 'interactome' provides insights into the functional landscape governing CE systems essential to bacterial growth, metabolism and drug resistance.
- Published
- 2018
- Full Text
- View/download PDF
40. [Age peculiarities in prostate cancer detection based on the prostate-specific antigen and its alterations control].
- Author
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Ponkratov SV, Kheyfets VK, and Kagan OF
- Subjects
- Humans, Male, Prevalence, Prostatic Neoplasms blood, Prostatic Neoplasms epidemiology, Sensitivity and Specificity, Age Factors, Prostate-Specific Antigen blood, Prostatic Neoplasms diagnosis
- Abstract
Data on epidemiology of a prostate cancer are presented in article, high prevalence and body height of a case rate cause relevance of researches on this oncopathology. It is shown that the number augmentation for the first time of the taped cases is bound including to the program of a screening of inspection of men by determination of level of prostates-specific antigen (PSA). Modern diagnostic methods of identification of modifications of PSA, possessing larger sensitivity and specificity concerning a prostate cancer are lit. The attention to change of level of PSA depending on age is focused that needs to be considered at diagnostics of malignant neoplasms of a prostate.
- Published
- 2017
41. Proteome-wide dataset supporting the study of ancient metazoan macromolecular complexes.
- Author
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Phanse S, Wan C, Borgeson B, Tu F, Drew K, Clark G, Xiong X, Kagan O, Kwan J, Bezginov A, Chessman K, Pal S, Cromar G, Papoulas O, Ni Z, Boutz DR, Stoilova S, Havugimana PC, Guo X, Malty RH, Sarov M, Greenblatt J, Babu M, Derry WB, Tillier ER, Wallingford JB, Parkinson J, Marcotte EM, and Emili A
- Abstract
Our analysis examines the conservation of multiprotein complexes among metazoa through use of high resolution biochemical fractionation and precision mass spectrometry applied to soluble cell extracts from 5 representative model organisms Caenorhabditis elegans, Drosophila melanogaster, Mus musculus, Strongylocentrotus purpuratus, and Homo sapiens. The interaction network obtained from the data was validated globally in 4 distant species (Xenopus laevis, Nematostella vectensis, Dictyostelium discoideum, Saccharomyces cerevisiae) and locally by targeted affinity-purification experiments. Here we provide details of our massive set of supporting biochemical fractionation data available via ProteomeXchange (PXD002319-PXD002328), PPIs via BioGRID (185267); and interaction network projections via (http://metazoa.med.utoronto.ca) made fully accessible to allow further exploration. The datasets here are related to the research article on metazoan macromolecular complexes in Nature [1].
- Published
- 2015
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42. Panorama of ancient metazoan macromolecular complexes.
- Author
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Wan C, Borgeson B, Phanse S, Tu F, Drew K, Clark G, Xiong X, Kagan O, Kwan J, Bezginov A, Chessman K, Pal S, Cromar G, Papoulas O, Ni Z, Boutz DR, Stoilova S, Havugimana PC, Guo X, Malty RH, Sarov M, Greenblatt J, Babu M, Derry WB, Tillier ER, Wallingford JB, Parkinson J, Marcotte EM, and Emili A
- Subjects
- Animals, Datasets as Topic, Humans, Protein Interaction Mapping, Reproducibility of Results, Systems Biology, Tandem Mass Spectrometry, Evolution, Molecular, Multiprotein Complexes chemistry, Multiprotein Complexes metabolism, Protein Interaction Maps
- Abstract
Macromolecular complexes are essential to conserved biological processes, but their prevalence across animals is unclear. By combining extensive biochemical fractionation with quantitative mass spectrometry, here we directly examined the composition of soluble multiprotein complexes among diverse metazoan models. Using an integrative approach, we generated a draft conservation map consisting of more than one million putative high-confidence co-complex interactions for species with fully sequenced genomes that encompasses functional modules present broadly across all extant animals. Clustering reveals a spectrum of conservation, ranging from ancient eukaryotic assemblies that have probably served cellular housekeeping roles for at least one billion years, ancestral complexes that have accrued contemporary components, and rarer metazoan innovations linked to multicellularity. We validated these projections by independent co-fractionation experiments in evolutionarily distant species, affinity purification and functional analyses. The comprehensiveness, centrality and modularity of these reconstructed interactomes reflect their fundamental mechanistic importance and adaptive value to animal cell systems.
- Published
- 2015
- Full Text
- View/download PDF
43. [ASSESSMENT OF EFFICIENCY OF TREATMENT OF OVERACTIVE BLADDER IN ELDERLY PATIENTS].
- Author
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Sosnovskiĭ SO, Kheĭfets VKh, and Kagan OF
- Subjects
- Adrenergic alpha-1 Receptor Antagonists administration & dosage, Age Factors, Aged, Aged, 80 and over, Dose-Response Relationship, Drug, Drug Therapy, Combination, Female, Follow-Up Studies, Humans, Male, Middle Aged, Muscarinic Antagonists administration & dosage, Prazosin administration & dosage, Solifenacin Succinate, Treatment Outcome, Urinary Bladder, Overactive diagnosis, Urinary Bladder, Overactive physiopathology, Urination drug effects, Prazosin analogs & derivatives, Quinuclidines administration & dosage, Tetrahydroisoquinolines administration & dosage, Urinary Bladder, Overactive drug therapy
- Abstract
The effectiveness of overactive bladder treatment with M-cholinoblocker solifenacin (Vesicare) as monotherapy and in combination with α1-andrenoblocker terazosin (Setegis) or bladdrer training in elderly patients was evaluated. The results of 12 weeks treatment in all treatment modality groups were comparable. Use of solifenacin in combination with α1-andrenoblocker in comparison to solifenacin monotherapy resulted in longer symptoms remission.
- Published
- 2015
44. [VARICOSE VEINS OF PELVIS IN ELDERLY WOMEN AS ACTUAL PROBLEM IN GERIATRICS].
- Author
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Litynskiĭ AV, Kataeva ED, Kheĭfetz VKh, Kagan OF, and Shaginian GG
- Subjects
- Age Factors, Aged, Female, Global Health, Humans, Incidence, Geriatrics, Pelvis blood supply, Varicose Veins epidemiology
- Abstract
The article presents an overview of the problem of varicose veins of pelvic (VVP) in elderly women. VVP is severe suffering to women, especially in the elderly, it promotes social exclusion, leads to psychoemotional disorders and occurrence of chronic pelvic pain due to pelvic varicose veins transformation. Despite the 150-year history of the disease, there is still no clear understanding of the clinical picture of the disease and, most importantly, how to treat this condition. In this regard, it is highly relevant research on timely diagnosis and appropriate treatment of VVP.
- Published
- 2015
45. The crystal structure of pyrimidine/thiamin biosynthesis precursor-like domain-containing protein CAE31940 from proteobacterium Bordetella bronchiseptica RB50, and evolutionary insight into the NMT1/THI5 family.
- Author
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Bajor J, Tkaczuk KL, Chruszcz M, Chapman H, Kagan O, Savchenko A, and Minor W
- Subjects
- Amino Acid Sequence, Crystallography, X-Ray, Protein Conformation, Protein Structure, Tertiary, Bacterial Proteins chemistry, Bordetella bronchiseptica, Evolution, Molecular
- Abstract
We report a 2.0 Å structure of the CAE31940 protein, a proteobacterial NMT1/THI5-like domain-containing protein. We also discuss the primary and tertiary structure similarity with its homologs. The highly conserved FGGXMP motif was identified in CAE31940, which corresponds to the GCCCX motif located in the vicinity of the active center characteristic for THi5-like proteins found in yeast. This suggests that the FGGXMP motif may be a unique hallmark of proteobacterial NMT1/THI5-like proteins.
- Published
- 2014
- Full Text
- View/download PDF
46. Fetal behavioral states are stable over daytime - evidence by longitudinal and cross-sectional fetal biomagnetic recordings.
- Author
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Sonanini A, Stingl K, Preissl H, Brändle J, Hoopmann M, Kagan O, Wallwiener D, Abele H, and Kiefer-Schmidt I
- Subjects
- Adult, Cross-Sectional Studies, Female, Humans, Longitudinal Studies, Magnetometry, Young Adult, Behavior, Fetus physiology, Pregnancy physiology
- Abstract
Aims: Fetal behavioral states can be distinguished by biomagnetic recordings. We performed a longitudinal and a cross-sectional study to address the question whether the distribution of fetal behavioral states changes during the daytime., Methods: For the longitudinal study, 32 magnetocardiographic recordings were performed on a singleton pregnancy on a weekly basis. On each examination day, two recordings were performed at different times between 25 and 40 weeks of gestation. For the cross-sectional study, fetal magnetocardiograms (fMCG) were recorded in a group of 32 singleton pregnancies matched for gestational age and daytime to the longitudinal study. The recordings were separated into two gestational age groups (less and more than 32 weeks). Fetal behavioral states were extracted from actocardiograms generated from MCG., Results: No significant differences in fetal behavioral state distribution were found between morning and afternoon recordings in either the longitudinal or the cross-sectional study., Conclusion: This is the first magnetographic approach to show that daytime does not influence the distribution of fetal behavioral states in standardized recordings of 30 min length. This result implies that fetal magnetography recordings at normal daytimes can be combined without a bias and future recordings can be conducted independently of daytime as long as the varying behavioral states are generally taken into account during analysis.
- Published
- 2014
- Full Text
- View/download PDF
47. [Peculiarities of clinical course, diagnostics and treatment of overactive bladder in men older than 60 years].
- Author
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Sosnovskiĭ SO, Kheĭfets VKh, and Kagan OF
- Subjects
- Aged, Aged, 80 and over, Drug Therapy, Combination, Humans, Male, Middle Aged, Prazosin administration & dosage, Prazosin pharmacology, Prazosin therapeutic use, Prostatic Hyperplasia complications, Prostatitis complications, Quinuclidines administration & dosage, Quinuclidines pharmacology, Solifenacin Succinate, Tetrahydroisoquinolines administration & dosage, Tetrahydroisoquinolines pharmacology, Treatment Outcome, Urinary Bladder, Overactive etiology, Urinary Bladder, Overactive metabolism, Urodynamics drug effects, Urological Agents administration & dosage, Urological Agents pharmacology, Prazosin analogs & derivatives, Quinuclidines therapeutic use, Tetrahydroisoquinolines therapeutic use, Urinary Bladder, Overactive diagnosis, Urinary Bladder, Overactive drug therapy, Urological Agents therapeutic use
- Abstract
The article presents the issues of the characteristics of the course, diagnostics and treatment of hyperactive urinary bubble in older men. Conservative treatment of urinary incontinence includes changes in lifestyle, behavioural and medical therapy with m-anticholinergic drugs. The combination solifenacini in a dose of 5 mg/day and α1-adrenoceptor blocking agent terasolini in a dose of 2 mg/day significantly improves the results of treatment and well tolerated.
- Published
- 2014
48. [Completion technology of pelvic surgery accompanying with cystectomy in elderly and senile patients].
- Author
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Krest'ianinov SS, Kostiuk IP, Shestaev AIu, Shostka KG, Vasil'ev LA, Kheĭfets VKh, Pavlenko AN, and Kagan OF
- Subjects
- Aged, Aged, 80 and over, Colorectal Neoplasms surgery, Comparative Effectiveness Research, Female, Humans, Lymph Node Excision, Male, Middle Aged, Postoperative Period, Treatment Outcome, Urinary Bladder Neoplasms surgery, Urogenital Neoplasms surgery, Cystectomy adverse effects, Cystectomy methods, Drainage adverse effects, Drainage methods, Pelvic Exenteration adverse effects, Pelvic Exenteration methods, Postoperative Complications diagnosis, Postoperative Complications prevention & control
- Abstract
Results of treatment of 204 elderly and senile patients who underwent cystprostatectomy or anterior pelvic exenteration are analyzed. A comparative analysis of two groups of patients whose operation ended with the traditional drainage through the anterior abdominal wall (n = 100), and bilateral perineal drainage (n = 104) is presented. Bilateral perineal drainage after operations on the pelvic organs, accompanied by cystectomy and extended lymphadenectomy in conjunction with the restoration of the peritoneum lateral pelvic walls, improves postoperative recovery of intestinal peristalsis, promotes an earlier reduction in the intensity of pain and morbidity in the early postoperative period. Perineal installation of drains is a simple in design and safe procedure. We recommend bilateral perineal drainage after operations on the pelvic organs, accompanied by cystectomy and extended lymphadenectomy.
- Published
- 2014
49. Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica.
- Author
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Kube M, Chernikova TN, Al-Ramahi Y, Beloqui A, Lopez-Cortez N, Guazzaroni ME, Heipieper HJ, Klages S, Kotsyurbenko OR, Langer I, Nechitaylo TY, Lünsdorf H, Fernández M, Juárez S, Ciordia S, Singer A, Kagan O, Egorova O, Petit PA, Stogios P, Kim Y, Tchigvintsev A, Flick R, Denaro R, Genovese M, Albar JP, Reva ON, Martínez-Gomariz M, Tran H, Ferrer M, Savchenko A, Yakunin AF, Yakimov MM, Golyshina OV, Reinhardt R, and Golyshin PN
- Subjects
- Alcanivoraceae genetics, Alcanivoraceae metabolism, Amino Acid Sequence, Bacterial Proteins genetics, Biodegradation, Environmental, Chromosome Mapping, Cold Temperature, Gammaproteobacteria classification, Gammaproteobacteria metabolism, Gene Transfer, Horizontal, Genome Size, Industrial Oils, Molecular Chaperones genetics, Molecular Sequence Data, Phylogeny, Protein Folding, Salinity, Sequence Analysis, DNA, Adaptation, Physiological genetics, Bacterial Proteins chemistry, Gammaproteobacteria genetics, Genome, Bacterial, Molecular Chaperones chemistry
- Abstract
Ubiquitous bacteria from the genus Oleispira drive oil degradation in the largest environment on Earth, the cold and deep sea. Here we report the genome sequence of Oleispira antarctica and show that compared with Alcanivorax borkumensis--the paradigm of mesophilic hydrocarbonoclastic bacteria--O. antarctica has a larger genome that has witnessed massive gene-transfer events. We identify an array of alkane monooxygenases, osmoprotectants, siderophores and micronutrient-scavenging pathways. We also show that at low temperatures, the main protein-folding machine Cpn60 functions as a single heptameric barrel that uses larger proteins as substrates compared with the classical double-barrel structure observed at higher temperatures. With 11 protein crystal structures, we further report the largest set of structures from one psychrotolerant organism. The most common structural feature is an increased content of surface-exposed negatively charged residues compared to their mesophilic counterparts. Our findings are relevant in the context of microbial cold-adaptation mechanisms and the development of strategies for oil-spill mitigation in cold environments.
- Published
- 2013
- Full Text
- View/download PDF
50. Identification of protein complexes in Escherichia coli using sequential peptide affinity purification in combination with tandem mass spectrometry.
- Author
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Babu M, Kagan O, Guo H, Greenblatt J, and Emili A
- Subjects
- Escherichia coli genetics, Escherichia coli metabolism, Escherichia coli Proteins genetics, Escherichia coli Proteins metabolism, Silver Staining methods, Escherichia coli chemistry, Escherichia coli Proteins isolation & purification, Tandem Mass Spectrometry methods
- Abstract
Since most cellular processes are mediated by macromolecular assemblies, the systematic identification of protein-protein interactions (PPI) and the identification of the subunit composition of multi-protein complexes can provide insight into gene function and enhance understanding of biological systems(1, 2). Physical interactions can be mapped with high confidence vialarge-scale isolation and characterization of endogenous protein complexes under near-physiological conditions based on affinity purification of chromosomally-tagged proteins in combination with mass spectrometry (APMS). This approach has been successfully applied in evolutionarily diverse organisms, including yeast, flies, worms, mammalian cells, and bacteria(1-6). In particular, we have generated a carboxy-terminal Sequential Peptide Affinity (SPA) dual tagging system for affinity-purifying native protein complexes from cultured gram-negative Escherichia coli, using genetically-tractable host laboratory strains that are well-suited for genome-wide investigations of the fundamental biology and conserved processes of prokaryotes(1, 2, 7). Our SPA-tagging system is analogous to the tandem affinity purification method developed originally for yeast(8, 9), and consists of a calmodulin binding peptide (CBP) followed by the cleavage site for the highly specific tobacco etch virus (TEV) protease and three copies of the FLAG epitope (3X FLAG), allowing for two consecutive rounds of affinity enrichment. After cassette amplification, sequence-specific linear PCR products encoding the SPA-tag and a selectable marker are integrated and expressed in frame as carboxy-terminal fusions in a DY330 background that is induced to transiently express a highly efficient heterologous bacteriophage lambda recombination system(10). Subsequent dual-step purification using calmodulin and anti-FLAG affinity beads enables the highly selective and efficient recovery of even low abundance protein complexes from large-scale cultures. Tandem mass spectrometry is then used to identify the stably co-purifying proteins with high sensitivity (low nanogram detection limits). Here, we describe detailed step-by-step procedures we commonly use for systematic protein tagging, purification and mass spectrometry-based analysis of soluble protein complexes from E. coli, which can be scaled up and potentially tailored to other bacterial species, including certain opportunistic pathogens that are amenable to recombineering. The resulting physical interactions can often reveal interesting unexpected components and connections suggesting novel mechanistic links. Integration of the PPI data with alternate molecular association data such as genetic (gene-gene) interactions and genomic-context (GC) predictions can facilitate elucidation of the global molecular organization of multi-protein complexes within biological pathways. The networks generated for E. coli can be used to gain insight into the functional architecture of orthologous gene products in other microbes for which functional annotations are currently lacking.
- Published
- 2012
- Full Text
- View/download PDF
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