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4. IQGAP1 mediates the communication between the nucleus and the mitochondria via NDUFS4 alternative splicing

Author

Papadaki, Vasiliki, primary, Erpapazoglou, Zoi, additional, Kokkori, Maria, additional, Rogalska, Malgorzata Ewa, additional, Potiri, Myrto, additional, Birladeanu, Andrada, additional, Tsakiri, Eleni N, additional, Ashktorab, Hassan, additional, Smoot, Duane T, additional, Papanikolopoulou, Katerina, additional, Samiotaki, Martina, additional, and Kafasla, Panagiota, additional

Published
2023
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5. Isoform changes of action potential regulators in the ventricles of arrhythmogenic phospholamban-R14del humanized mouse hearts

Author

Experimentele Afd. Cardiologie 2, Rogalska, Malgorzata E., Vafiadaki, Elizabeth, Erpapazoglou, Zoi, Haghighi, Kobra, Green, Lisa, Mantzoros, Christos S., Hajjar, Roger J., Tranter, Michael, Karakikes, Ioannis, Kranias, Evangelia G., Stillitano, Francesca, Kafasla, Panagiota, Sanoudou, Despina, Experimentele Afd. Cardiologie 2, Rogalska, Malgorzata E., Vafiadaki, Elizabeth, Erpapazoglou, Zoi, Haghighi, Kobra, Green, Lisa, Mantzoros, Christos S., Hajjar, Roger J., Tranter, Michael, Karakikes, Ioannis, Kranias, Evangelia G., Stillitano, Francesca, Kafasla, Panagiota, and Sanoudou, Despina

Published
2023

9. Nuclear IQGAP1 promotes gastric cancer cell growth by altering the splicing of a cell-cycle regulon in co-operation with hnRNPM

Author

Birladeanu, Andrada, Rogalska, Malgorzata, Potiri, Myrto, Papadaki, Vassiliki, Andreadou, Margarita, Kontoyiannis, Dimitris L, Erpapazoglou, Zoi, Lewis, Joe D, and Kafasla, Panagiota

Abstract

Heterogeneous nuclear ribonucleoproteins (hnRNPs) play crucial roles in alternative splicing, regulating the cell proteome. When they are miss-expressed, they contribute to human diseases. hnRNPs are nuclear proteins, but where they localize and what determines their tethering to nuclear assemblies in response to signals remain open questions. Here we report that IQGAP1, a cytosolic scaffold protein, localizes in the nucleus of gastric cancer cells acting as a tethering module for hnRNPM and other spliceosome components. One of the consequences of the IQGAP1-hnRNPM interaction is to change the alternative splicing of the anaphase promoting complex (ANAPC), subunit 10 and is required for the hnRNPM response to heat-induced stress signals. Together, IQGAP1 and hnRNPM promote gastric cancer cell growth, and their genetic depletion leads to cell cycle arrest and halts tumour progression. We propose that in response to stress-signals IQGAP1 translocates in the nucleus to coordinate gastric tumour growth by recruiting spliceosome components that drive cell cycle progression.

Published
2020
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12. AgtA, the dicarboxylic amino acid transporter of Aspergillus nidulans, is concertedly down-regulated by exquisite sensitivity to nitrogen metabolite repression and ammonium-elicited endocytosis

Author

Apostolaki, Angeliki, Erpapazoglou, Zoi, Harispe, Laura, Billini, Maria, Kafasla, Panagiota, Kizis, Dimosthenis, Peñalva, Miguel Ángel, Scazzocchio, Claudio, Sophianopoulou, Vicky, Apostolaki, Angeliki, Erpapazoglou, Zoi, Harispe, Laura, Billini, Maria, Kafasla, Panagiota, Kizis, Dimosthenis, Peñalva, Miguel Ángel, Scazzocchio, Claudio, and Sophianopoulou, Vicky

Abstract

We identified agtA, a gene that encodes the specific dicarboxylic amino acid transporter of Aspergillus nidulans. The deletion of the gene resulted in loss of utilization of aspartate as a nitrogen source and of aspartate uptake, while not completely abolishing glutamate utilization. Kinetic constants showed that AgtA is a high-affinity dicarboxylic amino acid transporter and are in agreement with those determined for a cognate transporter activity identified previously. The gene is extremely sensitive to nitrogen metabolite repression, depends on AreA for its expression, and is seemingly independent from specific induction. We showed that the localization of AgtA in the plasma membrane necessitates the ShrA protein and that an active process elicited by ammonium results in internalization and targeting of AgtA to the vacuole, followed by degradation. Thus, nitrogen metabolite repression and ammonium-promoted vacuolar degradation act in concert to downregulate dicarboxylic amino acid transport activity.

Published
2009

13. Defining the roles and interactions of PTB

Author

Kafasla, Panagiota, primary, Mickleburgh, Ian, additional, Llorian, Miriam, additional, Coelho, Miguel, additional, Gooding, Clare, additional, Cherny, Dmitry, additional, Joshi, Amar, additional, Kotik-Kogan, Olga, additional, Curry, Stephen, additional, Eperon, Ian C., additional, Jackson, Richard J., additional, and Smith, Christopher W.J., additional

Published
2012
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18. AgtA, the Dicarboxylic Amino Acid Transporter of Aspergillus nidulans , Is Concertedly Down-Regulated by Exquisite Sensitivity to Nitrogen Metabolite Repression and Ammonium-Elicited Endocytosis

Author

Apostolaki, Angeliki, primary, Erpapazoglou, Zoi, additional, Harispe, Laura, additional, Billini, Maria, additional, Kafasla, Panagiota, additional, Kizis, Dimosthenis, additional, Peñalva, Miguel Angel, additional, Scazzocchio, Claudio, additional, and Sophianopoulou, Vicky, additional

Published
2009
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20. Decoding the functions of post-transcriptional regulators in the determination of inflammatory states: focus on macrophage activation.

Author

Kafasla P, Karakasiliotis I, and Kontoyiannis DL

Subjects
Animals, Antigens, Surface metabolism, ELAV Proteins metabolism, ELAV-Like Protein 1, Epigenesis, Genetic, Heterogeneous Nuclear Ribonucleoprotein D0, Heterogeneous-Nuclear Ribonucleoprotein D metabolism, Inflammation genetics, Inflammation metabolism, Inflammation pathology, Macrophages immunology, Mice, MicroRNAs metabolism, Models, Molecular, RNA Processing, Post-Transcriptional, RNA, Messenger metabolism, RNA, Untranslated metabolism, RNA-Binding Proteins metabolism, Macrophages metabolism
Abstract

Inflammation involves a continuum of intercellular interactions and cellular responses targeting infectious or tissue damage while maintaining homeostasis. At its core, this continuum encompasses the alternating phenotypes of innate immune cells; each phenotype is typified by the expression of molecules which either support host defence or aid tissue restoration and the resolution of inflammation. The aberrant persistence of any such phenotype can drive chronic inflammatory pathology. For macrophages, these phenotypes arise as changes in cellular plasticity because of adaptation. As such their underlying gene expression programs may not be determined by robust transcriptomic and epigenetic programs but by more flexible means like post-transcriptional mechanisms affecting mRNA use. These mechanisms require the assemblies of RNA-binding proteins (RBPs) and non-coding RNAs onto specific elements on their RNA targets in Ribonucleoprotein particles (RNPs) which control mRNA maturation, turnover and translation. The collection of RNPs within a cell defines the ribonome, that is, a high order system of coordinative post-transcriptional determination. mRNAs involved in the definition of different macrophage activation phenotypes share elements of RBP recognition rendering them amenable to ribonomic regulation. The molecular features of their cognitive RBPs and the pathologies developing in the corresponding mouse mutants support their involvement in inflammatory reactions. We view this information in the context of macrophage activation states to propose that these states can be determined via differential--synergistic or antagonistic--RNP associations. In doing so, we substantiate the need for the use of systems platforms to model RNP hierarchies controlling the continuum of inflammation., (Copyright © 2012 Wiley Periodicals, Inc.)

Published
2012
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