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2. Epidemiology of Congenital Cholesteatoma

Author

Kadowaki, Yoshinori, primary, Ide, Shinsuke, additional, Nakamura, Takeshi, additional, Okuda, Takumi, additional, Shigemi, Hideto, additional, Hirano, Takashi, additional, Takahashi, Kuniyuki, additional, and Suzuki, Masashi, additional

Published
2023
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13. The Relationship between Werner Syndrome and Sinonasal Malignant Melanoma: Two Sibling Cases of Werner Syndrome with Malignant Melanoma

Author

Kadowaki, Yoshinori, Kodama, Satoru, Moriyama, Munehito, and Suzuki, Masashi

Subjects
Article Subject, otorhinolaryngologic diseases
Abstract

Werner syndrome (WS) is an autosomal recessive disease characterized by premature aging. Malignant tumors such as thyroid carcinoma and malignant melanoma occur frequently in WS patients. We describe 2 siblings with WS who suffered from sinonasal malignant melanoma (MM). Both patients initially experienced nasal obstruction and recurrent nasal bleeding and died within 2 years of the diagnosis of MM. Otolaryngologists should recognize that WS patients have a high risk for head and neck malignant disease, particularly sinonasal MM, even if they are aged below the expected age range and undergo periodic examinations. Furthermore, it is important that WS patients are aware that a prompt nasal examination is indicated if they experience continuous nasal obstruction or recurrent nasal bleeding.

Published
2017
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16. Interaction Between Regulatory T Cells and Antibody-Producing B Cells for Immune Responses at the Upper Respiratory Mucosa Against Nontypeable Haemophilus influenzae: In Vitro Assay Model

Author

Moon, Sung K., Kawauchi, Hideyuki, Oh, Seung-Ha, Hirano, Takashi, Kadowaki, Yoshinori, Matsunaga, Takayuki, Yoshinaga, Kazuhiro, Kawano, Toshiaki, Moriyama, Munehito, and Suzuki, Masashi

Abstract

Objectives: The aim of this study was to investigate the effect of regulatory T cells (Tregs) on B-cell immune responses against outer membrane protein (OMP) from nontypeable Haemophilus influenzae (NTHi) in vitro, to clarify its exact mechanism from an immunologic standpoint.Methods: Mice were vaccinated intranasally with OMP to induce OMP-specific immune responses in the nasal mucosa. Mononuclear cells (MNCs) were collected from the nasal mucosa, and Tregs and helper T (Th) cells were isolated separately from the spleens of those mice. Three different cell culture groups were allocated: MNCs cocultured with Tregs, MNCs cocultured with Th cells, and MNCs cultured alone. At 24 and 72 hours after cell culture, the concentrations of various cytokines and antibodies in culture supernatants were measured to assess the effects of Tregs and Th cells on B-cell responses. Cytokine levels and specific anti-OMP antibody levels in culture media were determined using enzyme-linked immunosorbent assay. CD69 or CD80 expression on B220-positive cells was detected using flow cytometric analysis.Results: Th1 and Th2 cytokine concentrations were significantly elevated in the 3 groups incubated with OMP from 24 to 72 hours. Additionally, interleukin-10 levels were significantly higher in the Treg and Th groups than in the control group. Levels of OMP-specific immunoglobulin A did not differ significantly among the groups. The ratios of CD69+B220+B2 cells were nearly the same in the 3 groups; however, the ratio of CD80+B220+B2 cells was higher in the control group than in the Treg and Th groups during incubation.Conclusions: Tregs and Th cells did not affect OMP-specific immunoglobulin A production in this study. However, these cells may partially inhibit B-cell functions, such as T-cell activation. These inhibitory effects may be related to interleukin-10.

Published
2019
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20. Interaction Between Regulatory T Cells and Antibody-Producing B Cells for Immune Responses at the Upper Respiratory Mucosa Against Nontypeable Haemophilus influenzae: In Vitro Assay Model.

Author

Hirano T, Kadowaki Y, Matsunaga T, Yoshinaga K, Kawano T, Moriyama M, and Suzuki M

Subjects
Animals, Antigens, CD metabolism, Antigens, Differentiation, T-Lymphocyte metabolism, B7-1 Antigen metabolism, Bacterial Outer Membrane Proteins, Cell Culture Techniques, Cytokines metabolism, Immunity, Mucosal, Lectins, C-Type metabolism, Mice, Mice, Inbred BALB C, Nasal Mucosa, B-Lymphocytes physiology, Haemophilus influenzae immunology, T-Lymphocytes, Regulatory physiology
Abstract

Objectives: The aim of this study was to investigate the effect of regulatory T cells (Tregs) on B-cell immune responses against outer membrane protein (OMP) from nontypeable Haemophilus influenzae (NTHi) in vitro, to clarify its exact mechanism from an immunologic standpoint., Methods: Mice were vaccinated intranasally with OMP to induce OMP-specific immune responses in the nasal mucosa. Mononuclear cells (MNCs) were collected from the nasal mucosa, and Tregs and helper T (Th) cells were isolated separately from the spleens of those mice. Three different cell culture groups were allocated: MNCs cocultured with Tregs, MNCs cocultured with Th cells, and MNCs cultured alone. At 24 and 72 hours after cell culture, the concentrations of various cytokines and antibodies in culture supernatants were measured to assess the effects of Tregs and Th cells on B-cell responses. Cytokine levels and specific anti-OMP antibody levels in culture media were determined using enzyme-linked immunosorbent assay. CD69 or CD80 expression on B220-positive cells was detected using flow cytometric analysis., Results: Th1 and Th2 cytokine concentrations were significantly elevated in the 3 groups incubated with OMP from 24 to 72 hours. Additionally, interleukin-10 levels were significantly higher in the Treg and Th groups than in the control group. Levels of OMP-specific immunoglobulin A did not differ significantly among the groups. The ratios of CD69 + B220 + B2 cells were nearly the same in the 3 groups; however, the ratio of CD80 + B220 + B2 cells was higher in the control group than in the Treg and Th groups during incubation., Conclusions: Tregs and Th cells did not affect OMP-specific immunoglobulin A production in this study. However, these cells may partially inhibit B-cell functions, such as T-cell activation. These inhibitory effects may be related to interleukin-10.

Published
2019
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