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The heavy metals content of bitter leaf (Vernonia amygdalina) grown along heavy traffic routes in Port Harcourt was determined using Shimadzu Atomic Absorption Spectrophotometer (Model 6650). Results obtained showed that the level of concentrations of heavy metals in bitter leaf cultivated along Port Harcourt‐Aba road were Fe: 2.96 mg/kg; Pb: 0.220 mg/kg; Cr: 0.030 mg/kg; Zn: 0.890 mg/kg; along Akpajo‐Eleme Road were: Fe: 3.43 mg/kg; Pb:0.405 mg/kg; Cr: 0.052 mg/kg; Zn: 1.08 mg/kg; and along Refinery‐Akpajo Road were, Fe: 3.27 mg/kg; Pb:0.350 mg/kg Cr: 0.041 mg/kg; Zn: 1.12 mg/kg; while the heavy metal concentrations of bitter leaf cultivated completely away from the road side (control) were Fe: 2.21 mg/kg; Pb:0.156 mg/kg; Cr: 0.024 mg/kg; Zn: 0.840 mg/kg. The order of heavy metals concentrations on heavy traffic routes was Fe > Zn > Pb > Cr while that of the control was Fe >Zn > Pb > Cr. A Chi‐square test of mean significant difference of concentration between the bitter leaf harvested from heavy traffic routes and control sample at 0.01 or 1% level was carried out, from the results obtained the null hypothesis (HO) was accepted.

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