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application/pdf, Paleopathological investigations of human remains from the Neolithic Hemudu culture in the lower reaches of the Yangtze River in southern East Asia were conducted to clarify the health status of people in early rice-farming societies. Our results show that the occurrence ratios of cribra orbitalia and enamel hypoplasia did not differ significantly between early rice farmers and hunter-gatherers. By contrast, the occurrence ratios of periosteal reactions, dental caries, and antemortem tooth loss in adults were higher among the early rice farmers. Based on these findings and the results of archaeological research on the Hemudu culture, it was suggested that: 1) the Hemudu culture adopted a diversified livelihood strategy that was not overly dependent on rice as a food resource, which did not lead to an extreme decline in health status, 2) the work in the rice fields or the working environment caused stress to the workers, and 3) the rice-farming society’s dietary habits led to a decline in oral health. Our results provide new paleopathological insights into the health status of early rice farmers in East Asia. However, the sample size of early rice farmers used in this study was small, and more data are needed to verify the validity of the views presented here.

Adeno-associated virus 6 has been proposed as a potential vector candidate for specific gene expression in pain-related dorsal root ganglion neurons, but this has not been confirmed in nonhuman primates. The aim of our study was to analyze the transduction efficiency and target specificity of this viral vector in the common marmoset by comparing with those in the rat. When green fluorescent protein-expressing serotype-6 vector was injected into the sciatic nerve, the efficiency of gene expression in dorsal root ganglion neurons was comparable in both species. We found that the serotype-6 vector was largely specific to the pain-related ganglion neurons in the marmoset as well as in the rat, whereas the serotype-9 vector resulted in contrasting effects in the two species. Neither AAV6 nor AAV9 resulted in DRG toxicity when administered via the sciatic nerve, suggesting this as a safer route of sensory nerve transduction than the currently used intrathecal or intravenous administrative routes. Furthermore, the adeno-associated virus 6 vector could be an optimal serotype for gene therapy for human chronic pain that has minimal effect on other somatosensory functions of dorsal root ganglion neurons.

広島大学(Hiroshima University), 博士(学術), Doctor of Philosophy, doctoral