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We assemble a property-level panel of appraiser-reported attributes associated with 4.6 million loan applications from 2013 to 2017 to test whether attributes were consistently reported. Appraisers have an incentive to misreport property attributes to justify higher appraised values to ensure that associated mortgage loans are approved. We focus on property transactions with multiple sets of attributes reported by the same appraiser within four quarters and find evidence consistent with an intent to inflate valuations through attribute misreporting. We find that strategic misreporting of attributes is prevalent across markets, and that highly leveraged borrowers whose appraisals had inconsistently reported attributes were 9.8 percent more likely to become seriously delinquent in their loan payments. [ABSTRACT FROM AUTHOR]

Currents and pressure records from the DeepLev mooring station and drifter data in the eastern Levantine Basin were analyzed to identify the dominant tidal constituents and their seasonal and depth variability. Harmonic and spectral analysis of seasonal segments of currents and pressure reveal key attributes of the tidal regime: (1) dominant semidiurnal sea level variability; (2) seasonal variation of semidiurnal and diurnal tides in both currents and pressure datasets; and (3) significant diurnal currents with weak semidiurnal currents across all seasons. The most dominant tidal constituent from the pressure dataset is the M2 (12.4 h). Results from pressure datasets align with previous models and observations of semidiurnal tides. In contrast, the diurnal tides are larger than previously reported by 8--9 cm in the winter and 1--2 cm in the summer. The surface current tidal regime differs from prior reports in the eastern Levantine Basin, with M2 magnitudes weaker by 1 cm s-1, while the diurnal tides (K1, O1) are 1--2 cms-1 larger. Seasonal segments showed seasonal differences in the local tidal regime's amplitudes. The most pronounced seasonal differences were with the K1 and S2 tides, with differences between winter and fall of 7 cmfor the K1 and 4 cm between summer and fall for the S2. We utilized the DeepLev datasets to compare a moored device with surface drifters near DeepLev by analyzing the M2 and S2 tides. Additionally, we examined data at different dataset lengths, considering the time constraints needed to resolve the tides adequately. Longer datasets improved the resolution of the tidal analysis and reduced amplitude leakages from nearby frequencies, resulting in a more realistic and accurate analysis of the tidal currents. Conversely, longer datasets resulted in fewer drifters remaining our study region for the allotted dataset length. From 32 available segments in 15 day dataset to 7 with a 30 day dataset, reducing the effectiveness of drifters as a local tidal research tool. [ABSTRACT FROM AUTHOR]

Simple Summary: Serum TSH suppression in the management of PTC patients will inevitably cause iatrogenic thyrotoxicosis. Our study challenges, for the first time, the role of human recombinant thyrotropin (rh-TSH) as a mitogen on PTC cell lines. For this study, both K1 and TPC-1 cells were treated with clinically relevant rh-TSH under various conditions. The expression levels of TSHR and thyroglobulin (Tg) were determined and, subsequently, the proliferation and migration were assessed. Additionally, cells were engineered to overexpress TSHR in order to multiply TSH signal transduction. Under the conditions employed, rh-TSH was inadequate for inducing either the proliferation or the migration rate of both transformed and non-transformed cells, while Tg expression was increased. We report that, clinically speaking, rh-TSH doses cannot induce proliferation and migration in PTC cell lines, thus questioning TSH suppression in PTC patients. Further research is warranted to dissect the underlying mechanisms. These results could translate into better PTC patient management. Thyrotropin (TSH) suppression is required in the management of patients with papillary thyroid carcinoma (PTC) to improve their outcomes, inevitably causing iatrogenic thyrotoxicosis. Nevertheless, the evidence supporting this practice remains limited and weak, and in vitro studies examining the mitogenic effects of TSH in cancerous cells used supraphysiological doses of bovine TSH, which produced conflicting results. Our study explores, for the first time, the impact of human recombinant thyrotropin (rh-TSH) on human PTC cell lines (K1 and TPC-1) that were transformed to overexpress the thyrotropin receptor (TSHR). The cells were treated with escalating doses of rh-TSH under various conditions, such as the presence or absence of insulin. The expression levels of TSHR and thyroglobulin (Tg) were determined, and subsequently, the proliferation and migration of both transformed and non-transformed cells were assessed. Under the conditions employed, rh-TSH was not adequate to induce either the proliferation or the migration rate of the cells, while Tg expression was increased. Our experiments indicate that clinically relevant concentrations of rh-TSH cannot induce proliferation and migration in PTC cell lines, even after the overexpression of TSHR. Further research is warranted to dissect the underlying molecular mechanisms, and these results could translate into better management of treatment for PTC patients. [ABSTRACT FROM AUTHOR]

The Digital Markets Act (DMA) and the Digital Services Act (DSA) are important regulations in the European Union (EU) that aim to create fair and safe digital environments. The effectiveness of these regulations depends on their enforcement. The DMA focuses on addressing the market power of large online platforms, while the DSA aims to regulate digital services more broadly. The principle of effectiveness is crucial in assessing compliance with these laws. The effective implementation and enforcement of the DMA and the DSA require the involvement of various actors, including enforcement authorities, national regulators, third parties, and the European Commission. Private enforcement mechanisms, such as representative actions, can complement public enforcement efforts. Overall, effective enforcement is essential for achieving the objectives of these regulations and ensuring a fair and safe digital environment. [Extracted from the article]

To attain sustainable development, a pertinent question that needs to be answered is whether good governance leads to financial inclusion (FI) and whether bank stability matters. This study examines how financial stability modulates the link between governance and FI in the Nordic-Baltic region between 2004 and 2021. Using a mixed effect model, we observed a positive causal relationship between governance and FI and bank stability modulate this causality. These findings are robust to alternative assumptions and model specifications. Interestingly, we found that good governance helps foster economic growth and social equality. The study further highlights the need for digital financial education to overcome the phobia of digital products as a priority policy agenda. Overall, this finding supports arguments in the literature that for FI to be sustainable, inequality should be low as income levels and well-being increase.

Copyright of Sozialer Fortschritt is the property of Duncker & Humblot GmbH and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

El objeto de este estudio fue valorar la efectividad del remate del receptor delantero en función de su participación en la transición recepción-ataque y de la rotación del equipo, así como en función de la interacción entre ambas. La muestra se extrajo de 29 partidos jugados entre 2012 y 2016 por selecciones nacionales masculinas de máximo nivel mundial. Las variables estudiadas fueron: la rotación de equipo receptor, la existencia de transición recepción-ataque y el rendimiento del remate. En el análisis de datos, se describieron la media, la desviación típica y la efectividad; además de emplear ji al cuadrado de Pearson y modelos de regresiones ordinales para determinar la influencia de la transición, la rotación del equipo y la posición del colocador sobre el rendimiento del remate. El nivel de significación fue establecido en p = .05. Los resultados mostraron un mejor rendimiento en remate del receptor delantero cuando no realizó transición y el colocador se encontró en posición zaguera, especialmente en las rotaciones RT1 y RT5. También se encontró un mejor rendimiento del remate cuando se realizó la transición con colocador delantero, en relación con colocador zaguero. El peor rendimiento en remate se produjo cuando existió transición y el equipo se encontraba en RT6. Como conclusión, el rendimiento del remate del receptor delantero se ve afectado por la interacción entre la transición y la rotación del equipo en K1; ya sea estudiando las rotaciones de manera individual, o de manera integrada en función de la posición del colocador.

Kematian ibu merupakan masalah kesehatan masyarakat yang penting di atasi di negara berkembang. Penyebab kematian Ibu disebabkan dua faktor yakni penyebab langsung (direct obstetric) dan penyebab tidak langsung (indirect obstetric). Sesuai dengan Penilaian Kinerja Puskesmas Aur (PKP) tahun 2020 menunjukkan bahwa target K1 ialah 93% dan K4 ialah 85% sedangkan pencapaian K1 hanya 79,3% dan K4 hanya 50,2%. Penyebab langsung seperti faktor medis dapat dipastikan seperti perdarahan, eklamsia, partus tidak normal, sedangkan penyebab kematian tidak langsung (indirect obstetric) tidak mudah dipastikan penyebabnya dan membutuhkan pemeriksaan khusus oleh tenaga medis. Tujuan kegiatan ini melakukan upaya peningkatan kunjungan kehamilan di wilayah kerja Puskesmas Sungai Aur agar mencapai target, sehingga penyebab tingginya angka kematian ibu dapat dicegah. Metode dari kegiatan ini adalah dengan mengimplementasikan penyuluhan untuk meningkatkan kunjungan ibu hamil ke puskesmas dan memenuhi peningkatan target yang diharapkan baik K1 dan K4. Penyuluhan berkesinambungan dengan meningkatkan partisipasi serta kesadaran ibu terhadap pentingnya pemeriksaan kehamilan di fasilitas pelayanan kesehatan oleh tenaga kesehatan dilaksanakan di puskesmas, meningkatkan pencapaian K1 dan K4. Ibu hamil yang membutuhkan pertolongan segera, apalagi saat covid 19 masih berlangsung, dilaksanakan inovasi dengan menjemput langsung ke rumah pasien. Hasil dari kegiatan ini terlaksananya penyuluhan mengenai pentingnya kunjungan kehamilan, dan terlaksananya pembagian leaflet ke ibu hamil, serta mengegah resiko meningkatnya AKI. Saran penyuluhan tetap dilakukan, kesiapan tenaga dan sarana mengatasi masalah persalinan ibu dilestarikan.

The aim of this study was to assess the effectiveness of the front receiver's spike according to their participation in the reception-attack transition and the team's rotation, in addition to the interaction between the two. The sample was drawn from 29 matches played between 2012 and 2016 by top-level men's national teams. The variables studied were: the rotation of the receiving team, the existence of receptionattack transition and spike performance. In the data analysis, mean, standard deviation and effectiveness were described, and Pearson's chi-squared and ordinal regression models were used to determine the influence of transition, team rotation and setter position on spike performance. The significance level was set at p = .05. The results showed an improved spike performance by the front receiver when they did not transition and the setter was in the back position, especially in the RT1 and RT5 rotations. Improved spike performance was also found when the transition was made by a front setter, compared to a back setter. The worst spike performance occurred when there was transition and the team was in RT6. In conclusion, the spike performance of the front receiver is affected by the interaction between transition and team rotation in K1; either when studying the rotations individually, or comprehensively according to the position of the setter. [ABSTRACT FROM AUTHOR]

Hypervirulent Klebsiella pneumoniae (hvKp) is a common cause of pyogenic liver abscesses in Asia but is quite uncommon in North America. Among the cases described in North America, only occasional reports have described molecular strain typing to confirm the K1 strain as the causative agent. We report a 56-year-old Hispanic female with no previous intra-abdominal pathology and no recent travel, who presented with subacute abdominal pain and developed bacteremia and monomicrobial pyogenic liver abscess due to a community-acquired K1 serotype K. pneumoniae isolate. In this case, the infection was recognized early, so the patient was successfully treated with percutaneous drainage and prolonged antibiotic therapy. Hvkp can cause severe invasive disease with high morbidity and mortality, and the recent emergence of multidrug resistance in these strains poses a serious threat to public health. In addition, the isolation of a K1 K. pneumoniae strain from a cryptogenic liver abscess in a Hispanic patient with no epidemiologic risk factors raises concern for a wider spread of the hypervirulent strain beyond Asian populations. Therefore, a high index of suspicion for hvKp infection in the Hispanic population can be crucial as the hypervirulent strain is likely to cause severe metastatic infection with significant morbidity and mortality.

Despite prosecutors' difficulties in proving corporate bribery, nearly all enforcement actions end with a settlement at the pretrial stage. Compared to court proceedings, settlement-based enforcement provide prosecutors with flexibility to reward offenders' self-reporting and cooperation, and reach quicker conclusions to complex cases. In this article, we explain, such enforcement needs regulation to minimize potentially harmful side-effects. When the difference between a court and settlement sanction exceeds a certain size, the alleged offender accepts a settlement regardless of actual responsibility of misconduct. For the prosecutor, the option of offering a lenient settlement means weaker incentives to ascertain the material facts of the case. Society receives less information about the blameworthy act, little opportunity to evaluate the sanction, and less reason to expect sanctions to deter bribery. We show why such consequences result in under-deterrence of bribery and weaker rule of law. The use of settlement may have a self-escalating effect because the enforcement mode can reduce the predictability of the law, while a defendant's inclination to accept a settlement offer depends on the predictability of the law. Our results suggest that United Kingdom's current escalation of enforcement of corporate bribery laws will lead to a mixture of settlements and court decisions, while in the United States firms will continue to negotiate settlements as if there were no opportunity to have their cases tested in court. [ABSTRACT FROM AUTHOR]

This research covered the palliative material distribution project in Lagos State, Nigeria, during the lockdown and restriction on movement because of COVID-19 pandemic. The study engaged reports from extant literature and tertiary data sources such as the newspapers, the Internet, and website source. Relevant data gathered from these sources were conceptually analysed and discussed along with extant literature. The research also adopted the use of rich pictures for further presentation of the research. A key finding from the research is the need to redesign the distribution process and consider proper structuring of the project. And further engagement with the affected stakeholders to address crucial issues such as marginalisation that can defeat the purpose, which is to emancipate the affected indigents of the State from the harrowing effects of COVID-19 lockdown and other emergencies-related critical incidences such as starvation and criminalities. The study proposed a model for a resilience approach to effectively address the current palliative distribution process challenges in Lagos State, the epicentre of COVID-19 in Nigeria. [ABSTRACT FROM AUTHOR]

Hypervirulent Klebsiella pneumoniae (hvKp) is a common cause of pyogenic liver abscesses in Asia but is quite uncommon in North America. Among the cases described in North America, only occasional reports have described molecular strain typing to confirm the K1 strain as the causative agent. We report a 56-year-old Hispanic female with no previous intra-abdominal pathology and no recent travel, who presented with subacute abdominal pain and developed bacteremia and monomicrobial pyogenic liver abscess due to a community-acquired K1 serotype K. pneumoniae isolate. In this case, the infection was recognized early, so the patient was successfully treated with percutaneous drainage and prolonged antibiotic therapy. Hvkp can cause severe invasive disease with high morbidity and mortality, and the recent emergence of multidrug resistance in these strains poses a serious threat to public health. In addition, the isolation of a K1 K. pneumoniae strain from a cryptogenic liver abscess in a Hispanic patient with no epidemiologic risk factors raises concern for a wider spread of the hypervirulent strain beyond Asian populations. Therefore, a high index of suspicion for hvKp infection in the Hispanic population can be crucial as the hypervirulent strain is likely to cause severe metastatic infection with significant morbidity and mortality. [ABSTRACT FROM AUTHOR]

ABSTRACT K1 represents a heterodimeric A/B toxin secreted by virus-infected Saccharomyces cerevisiae strains. In a two-staged receptor-mediated process, the ionophoric activity of K1 leads to an uncontrolled influx of protons, culminating in the breakdown of the cellular transmembrane potential of sensitive cells. K1 killer yeast necessitate not only an immunity mechanism saving the toxin-producing cell from its own toxin but, additionally, a molecular system inactivating the toxic α subunit within the secretory pathway. In this study, different derivatives of the K1 precursor were constructed to analyze the biological function of particular structural components and their influence on toxin activity as well as the formation of protective immunity. Our data implicate an inactivation of the α subunit during toxin maturation and provide the basis for an updated model of K1 maturation within the host cell’s secretory pathway. IMPORTANCE The killer phenotype in the baker’s yeast Saccharomyces cerevisiae relies on two double-stranded RNA viruses that are persistently present in the cytoplasm. As they carry the same receptor populations as sensitive cells, killer yeast cells need—in contrast to various bacterial toxin producers—a specialized immunity mechanism. The ionophoric killer toxin K1 leads to the formation of cation-specific pores in the plasma membrane of sensitive yeast cells. Based on the data generated in this study, we were able to update the current model of toxin processing, validating the temporary inactivation of the toxic α subunit during maturation in the secretory pathway of the killer yeast.

Three-dimensional (3D) cell culture is well documented to regain intrinsic metabolic properties and to bettermimic the in vivo situation than two-dimensional (2D) cell culture. Particularly, proline metabolism is critical for tumorigenesis since pyrroline-5-carboxylate (P5C) reductase (PYCR/P5CR) is highly expressed in various tumors and its enzymatic activity is essential for in vitro 3D tumor cell growth and in vivo tumorigenesis. PYCR converts the P5C intermediate to proline as a biosynthesis pathway, whereas proline dehydrogenase (PRODH) breaks down proline to P5C as a degradation pathway. Intriguingly, expressions of proline biosynthesis PYCR gene and proline degradation PRODH gene are up-regulated directly by c-Myc oncoprotein and p53 tumor suppressor, respectively, suggesting that the proline-P5C metabolic axis is a key checkpoint for tumor cell growth. Here, we report a metabolic reprogramming of 3D tumor cell growth by oncogenic Kaposi’s sarcoma-associated herpesvirus (KSHV), an etiological agent of Kaposi’s sarcoma and primary effusion lymphoma. Metabolomic analyses revealed that KSHV infection increased nonessential amino acid metabolites, specifically proline, in 3D culture, not in 2D culture. Strikingly, the KSHV K1 oncoprotein interacted with and activated PYCR enzyme, increasing intracellular proline concentration. Consequently, the K1-PYCR interaction promoted tumor cell growth in 3D spheroid culture and tumorigenesis in nude mice. In contrast, depletion of PYCR expression markedly abrogated K1-induced tumor cell growth in 3D culture, not in 2D culture. This study demonstrates that an increase of proline biosynthesis induced by K1-PYCR interaction is critical for KSHV-mediated transformation in in vitro 3D culture condition and in vivo tumorigenesis. [ABSTRACT FROM AUTHOR]

Membrane lipids are essential participants in cellular events, but only a small number of lipid‐interacting proteins have been characterized. Taking advantage of the small genome (~270 genes) of the vaccinia virus, we screened for soluble lipid‐binding proteins and found 27 proteins to be soluble after expression in Escherichia coli. Among them, 4 proteins were found to strongly bind to the total bovine brain lipid extract (Folch I fraction) that contained large amounts of phosphatidylserine in vitro. Out of the 4 proteins, 3 were unique proteins to viruses. Another protein, K1, solely contained an ankyrin repeat domain (ARD). ARD is conserved in large numbers of proteins in bacteria, archaea, eukaryotes and viruses, suggesting the possibilities of the membrane binding of ARDs in varieties of proteins. Furthermore, K1 deformed the lipid membrane dependently on the charged lipids. The tubulation and membrane binding was enhanced with increased negative membrane charge from phosphatidylinositol 4,5‐bisphosphate (PI(4,5)P2). The basic amino acid residues in the ARD were essential for membrane deformation, suggesting electrostatic interactions between K1 and the membrane for membrane deformation. [ABSTRACT FROM AUTHOR]

The convergence of carbapenem-resistance and hypervirulence genes in Klebsiella pneumoniae has led to the emergence of highly drug-resistant superbugs capable of causing invasive disease. We analyzed 556 carbapenem-resistant K. pneumoniae isolates from patients in Singapore hospitals during 2010-2015 and discovered 18 isolates from 7 patients also harbored hypervirulence features. All isolates contained a closely related plasmid (pKPC2) harboring blaKPC-2, a K. pneumoniae carbapenemase gene, and had a hypervirulent background of capsular serotypes K1, K2, and K20. In total, 5 of 7 first patient isolates were hypermucoviscous, and 6 were virulent in mice. The pKPC2 was highly transmissible and remarkably stable, maintained in bacteria within a patient with few changes for months in the absence of antimicrobial drug selection pressure. Intrapatient isolates were also able to acquire additional antimicrobial drug resistance genes when inside human bodies. Our results highlight the potential spread of carbapenem-resistant hypervirulent K. pneumoniae in Singapore. [ABSTRACT FROM AUTHOR]

Using unique internal bank data on ex ante appraised liquidation and market values of assets pledged as collateral in sixteen countries, we show that laws and institutions that strengthen creditor protection increase expected recovery rates for collateral. Stronger creditor protection increases expected recovery rates for movable collateral relative to immovable collateral and shifts the composition of collateral toward movable assets, thereby increasing debt capacity through both higher loan-to-values and attenuating the creditor's liquidation bias. Our results suggest that the recovery rate for collateral is an important first-stage mechanism through which creditor protection can improve contracting efficiency and enhance access to credit. Received September 17, 2018; editorial decision July 9, 2019 by Editor Andrew Ellul. [ABSTRACT FROM AUTHOR]

The killer phenomenon in yeast (Saccharomyces cerevisiae) not only provides the opportunity to study host-virus interactions in a eukaryotic model but also represents a powerful tool to analyze potential coadaptional events and the role of killer yeast in biological diversity. Although undoubtedly having a crucial impact on the abundance and expression of the killer phenotype in killer-yeast harboring communities, the influence of a particular toxin on its producing host cell has not been addressed sufficiently. In this study, we describe a model system of two K1 killer yeast strains with distinct phenotypical differences pointing to substantial selection pressure in response to the toxin secretion level. Transcriptome and lipidome analyses revealed specific and intrinsic host cell adaptions dependent on the amount of K1 toxin produced. High basal expression of genes coding for osmoprotectants and stress-responsive proteins in a killer yeast strain secreting larger amounts of active K1 toxin implies a generally increased stress tolerance. Moreover, the data suggest that immunity of the host cell against its own toxin is essential for the balanced virus-host interplay providing valuable hints to elucidate the molecular mechanisms underlying K1 immunity and implicating an evolutionarily conserved role for toxin immunity in natural yeast populations. IMPORTANCE The killer phenotype in Saccharomyces cerevisiae relies on the cytoplasmic persistence of two RNA viruses. In contrast to bacterial toxin producers, killer yeasts necessitate a specific immunity mechanism against their own toxin because they bear the same receptor populations as sensitive cells. Although the killer phenomenon is highly abundant and has a crucial impact on the structure of yeast communities, the influence of a particular toxin on its host cell has been barely addressed. In our study, we used two derivatives secreting different amount of the killer toxin K1 to analyze potential coadaptional events in this particular host/virus system. Our data underline the dependency of the host cell's ability to cope with extracellular toxin molecules and intracellular K1 molecules provided by the virus. Therefore, this research significantly advances the current understanding of the evolutionarily conserved role of this molecular machinery as an intrinsic selection pressure in yeast populations. [ABSTRACT FROM AUTHOR]

Copyright of ORDO: Jahrbuch für die Ordnung von Wirtschaft und Gesellschaft is the property of De Gruyter and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Abstract Background Electrochemotherapy (ECT) has shown to be an effective treatment for cutaneous and subcutaneous Kaposi sarcoma (KS) lesions. However, no study has investigated the impact of ECT treatment on the kinetics of human herpesvirus type 8 (HHV8), which is considered the necessary causal agent of KS. We aimed to evaluate HHV8 viral load and expression levels in patients affected by classic KS who received one or more ECT treatments and have been followed semi annually for up to four years. Methods A total of 27 classic KS patients were enrolled in this study. Tumour biopsies and blood samples were obtained before ECT treatment. Additional blood samples were collected at six month intervals for 12–48 months. HHV8 viral load and expression profiles of latent (ORF72 and ORF73) and lytic (K2, K8, K8.1, K10/K10.1, K10.5/K10.6 and ORF16) genes were assessed in all samples by real-time PCR. HHV8 ORF26 and K1 regions were amplified and subjected to direct nucleotide sequencing followed by phylogenetic analysis for variant identification. Results All KS biopsies and 46.4% of peripheral blood mononuclear cells (PBMCs) collected before ECT treatment were positive for HHV8 DNA. Viral load ranged from 0.02 to 2.3 copies per cell in KS lesions and 3.0 × 10−7 to 6.9 × 10−4 copies per cell in PBMCs. Overall, latent ORF72 and ORF73 as well as lytic K2, K8 and K10/K10.1 were expressed in all KS biopsies. ORF16 mRNA was detected in 71.4% and both K8.1 and K10.5/K10.6 mRNAs in 57.1% of KS samples. The ORF72, ORF73 and K2 transcripts were amplified in 37.5%, 25% and 25% of PBMCs collected before ECT, respectively. After the first ECT session, complete response was achieved in 20 out of 27 (74.1%) patients and HHV8 DNA was detected in four out of 27 (14.8%) PBMC samples at six month follow up. Phylogenetic analysis of ORF26 amplimers showed that most viral variants belonged to A/C (82.3%), and few to C2 (5.9%) or C3 (11.8%) subtype. The K1/VR1 variants fell into A (33.3%) and C (66.7%) HHV8 clade. No correlation was found between HHV8 subtypes and ECT complete response. Conclusions ECT therapy has a significant effect on HHV8 kinetics in patients with classic KS. The complete remission of patients was accompanied by clearance of circulating virus.