Search

Pullet chicks were reared in isolation; all except a control group were variously vaccinated against infectious bronchitis. Individual haemagglutination inhibition (HI) antibody responses were measured from 1 day to 38 weeks old when all birds were challenged with virulent infectious bronchitis virus, and egg production recorded for a further 5 week period. In the controls HI titres remained low until challenge: this caused a loss of 15.1 eggs/hen. In birds injected with oil emulsion killed vaccine (OEV) at 3 and 16 weeks old, the serological response was uniformly high but on challenge the loss in egg production was 2.9 eggs/bird. Birds given H120 live vaccine at 3 weeks and H52 live vaccine at 15 weeks old had a low (23%) individual rate of serological response to the latter, and egg production after challenge was 3.63 eggs/hen less than before. In birds given H120 live vaccine at 3 weeks and emulsion killed vaccine at 16 weeks old, 100% serological response to the latter occurred and egg production was unaffected by challenge. A further group also received H120 and H52 live vaccines at 3 and 15 weeks old respectively: however, they were then subdivided into four groups and injected with emulsion vaccine at either 17, 19, 21 or 23 weeks old. Their response to H52 vaccine was variable. The proportion of birds in each sub-group responding serologically to subsequent vaccination with OEV was 45, 65, 73 and 92% respectively. After challenge egg production in these four sub-groups was reduced by 1.92, 1.15, 0.94 and 1.55 eggs/bird respectively. It is concluded that response to oil emulsion infectious bronchitis vaccine can be impaired if it is used within 8 weeks of H52 live vaccine. Best results are achieved where birds are given a primary dose of H120 live vaccine at 3 weeks old followed by emulsion vaccine 12-16 weeks later. Use of the less attenuated H52 strain of live vaccine before emulsion killed vaccine is contra-indicated.