1. HIV-1-specific cell-mediated immune responses induced by DNA vaccination were enhanced by mannan-coated liposomes and inhibited by anti-interferon-gamma antibody
- Author
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Norihisa Ishii, Eiichi Okada, Sumako Toda, Kenji Hamajima, Kenji Okuda, H. Arai, K.-I. Kusakabe, Itsuo Gorai, and K. Nishioka
- Subjects
Cytotoxicity, Immunologic ,medicine.medical_treatment ,Immunology ,chemical and pharmacologic phenomena ,HIV Antibodies ,DNA vaccination ,Mannans ,Interferon-gamma ,Mice ,Immune system ,Adjuvants, Immunologic ,Immunity ,Vaccines, DNA ,medicine ,Animals ,Immunology and Allergy ,Hypersensitivity, Delayed ,Cationic liposome ,Interferon gamma ,Mannan ,AIDS Vaccines ,Immunity, Cellular ,Mice, Inbred BALB C ,biology ,Chemistry ,Antibodies, Monoclonal ,carbohydrates (lipids) ,Liposomes ,HIV-1 ,biology.protein ,Cytokines ,Antibody ,Adjuvant ,Research Article ,medicine.drug - Abstract
The adjuvant effect of mannan-coated liposomes on human immunodeficiency virus type-1 (HIV-1) DNA vaccine and the mechanism of this enhancement were studied. Coating of cationic liposomes with mannan significantly enhanced the ability of this vaccine to induce an HIV-specific delayed-type hypersensitivity (DTH) response. HIV-specific cytotoxic T-cell (CTL) activity elicited by DNA vaccination was also significantly enhanced with the mannan-liposome cocktail. This mannan-liposome-mediated activity was greatly inhibited by in vivo injection of anti-interferon (IFN)-gamma antibody, which suggests that IFN-gamma plays an important role in this HIV-specific immune response. The results of both isotype-specific antibody and cytokine analysis revealed that mannan-liposome-mediated DNA vaccination enhances Th1-mediated immunity.
- Published
- 1997
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