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Background: Tumor associated antigen are glycoproteins and glycolipids expressed on the surface or in the cytoplasm of tumor cells. The antigenic components are shed from the tumor cells into the tissue culture medium or blood or other human body fluids like human milk. Objective: In the present study, investigate the serological assay of the antigen recognized by CAMA3C8 in patients with carcinoma of the breast. Methods: The study is desgriptive cross section study.The study was divited into four groups based on the expression of tumor associated antigen. Results: In breast cancer CAMA3C8 levels were significantly increased in stage 4 when compared with stage 1 breast cancer cell line. Conclusion: In the present study, we conclude that significantly recognized CAMA3C8 defined antigens in breast cancer.

Aim: The present study was conducted to investigate the hepatoprotective effect of enzymatic changes in vitamin D combination with Liv-52 on CCl4 induced liver toxicity in Wistar rats. Background: The central organ of liver plays an essential vital role in the metabolism, and the liver is called as the metabolic “engine-room of the body”. Therefore, to maintain a healthy liver is a crucial factor for overall health and well being. The liver is the central organ for pharmaceutical drug or chemicals and xenobiotic detoxification metabolism, which regulates most of medication and xenobiotic-related toxic activity. High metabolic and synthetic activity in this organ is an important place for the generation of free radicals. Materials and Methods: Adult male Albino Wistar rats weighing 150-250g were used in this study. The rats were split into six groups, each consisted of six rats. CCl4 were admitted two days in a week for five weeks. Liver disease associated animals were treated with Vitamin D and Liv-52 for 5 weeks. Results: The levels of AST, ALT, ALP, γ-GT, and AFP were significantly reduced in vitamin D, and Liv-52 treated animals when compared with CCl4 induced animals. Moreover, the levels of Vitamin D and Liv-52, a good indicator of restoring the liver architecture, were also reversed in the damage after treatment. Conclusion: The results of the present study indicate that the combination drugs were more hepatoprotective effect when compared with the individual.

Background and Objectives: Stem cells from human exfoliated deciduous teeth (SHEDs) have been demonstrated as a novel population of adult stem cells capable of multi-differentiation potential. Methods: Study samples comprise of 30 extracted exfoliating primary teeth collected from children aged 6 to 14 years. After attaining the required cell passage, flowcytometric analysis and trilineage differentiation was done to characterize SHEDs. Further SHEDs were differentiated into Islet like cell aggregates (ICAs) using Serum free media A,B&C. Differentiated ICAs were characterized by RT-PCR, immunocytochemistry, DTZ stain and insulin assay. Results: Flowcytometric analysis of SHEDs showed expression of positive markers CD73, CD90 while no expression of negative markers CD34, CD45 and HLA-DR. Isolated SHEDs had the potential to differentiate into tri-lineages and ICAs. RT-PCR analysis of derived ICAs showed up-regulated expression of GAPDH, insulin, Glut2, PDX1 and PAX6. Immunofluorescence analysis gave expression of Ngn3, Isl-1, C-peptide, Glut2 and PDX1. DTZ stained positive on derived ICAs.Insulin secretion of SHED derived ICAs were measured 26 ± 6 MIU/L at basal glucose level, 128±3 MIU/L and 240 ±9 MIU/L at stimulated glucose level which gave a statistically significant difference in mean value of insulin secreted in different concentration of glucose (p