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Your search keyword '"K. Kromphardt"' showing total 31 results
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31 results on '"K. Kromphardt"'

1. Coupling Microplate-Based Antibacterial Assay with Liquid Chromatography for High-Resolution Growth Inhibition Profiling of Crude Extracts: Validation and Proof-of-Concept Study with Staphylococcus aureus

Author

Hamidreza Ardalani, Syariful Anam, Kresten J. K. Kromphardt, Dan Staerk, and Kenneth T. Kongstad

Subjects
dye-free microplate assay, antibacterial, high-resolution antibacterial profiling, natural products, Staphylococcus aureus, Organic chemistry, QD241-441
Abstract

With the identification of novel antibiotics from nature being pivotal in the fight against human pathogenic bacteria, there is an urgent need for effective methodologies for expedited screening of crude extracts. Here we report the development and validation of a simple and dye-free antimicrobial assay in 96-well microplate format, for both determination of IC50 values and high-resolution inhibition profiling to allow pin-pointing of bioactive constituents directly from crude extracts. While commonly used antimicrobial assays visualize cell viability using dyes, the developed and validated assay conveniently uses OD600 measurements directly on the fermentation broth. The assay was validated with an investigation of the inhibitory activity of DMSO against Staphylococcus aureus, temperature robustness, interference by coloured crude extracts as well as inter-day reproducibility. The potential for high-resolution S. aureus growth inhibition profiling was evaluated on a crude extract of an inactive Alternaria sp., spiked with ciprofloxacin.

Published
2021
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4. Schröer: Physics and its Fifth Dimension: Society/Marsal: Russisch für Mathematiker, Physiker und Ingenieurwissenschaftler/Schailers: Meyers Handbuch über das Weltall/Hurrle: Technical Dictionary of Vacuum Physics and Vacuum Technology/Blochinzew: Space a

Author

W. A. P. Luck, K. Strubecker, K. Kromphardt, H. Adam, K. Kraus, H. Rechenberg, R. Meinhardt, R. Haensel, W. Heywang, and E. Brüche

Subjects
Physics, Gravitation, Theoretical physics, Dimension (vector space), Spacetime, Particle, Kinematics, Electronic band structure, Spectroscopy, Mathematical physics
Published
1974

17. Buchholz: Die große Transformation/Wheeler: Einsteins Vision/Clagett: Nicole Oresme and the Medieval Geometry of Qualities and Motions/Weninger: Einheiten, Größen und Skalenwerte/Gardner: Das gespiegelte Universum/Ajzenberg-Selove, Lauritsen: Energy Level

Author

D. Lilienthal, W. Döring, M. Diem, Karl Strubecker, K. Kromphardt, and Herwig Schopper

Subjects
Energy (psychological), Light nucleus, Transformation (function), Philosophy, FAUST, computer, computer.programming_language, Mathematical physics
Published
1969

31. [No human dignity for patients].

Author

Kromphardt K

Subjects
Hospitals, Psychiatric, Humans, Mental Disorders, Nursing, Patients, Prejudice
Published
1967

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