1. Antifungal activity of lichen extracts and compounds against Alternaria brassicola
- Author
-
N Blond, Joël Boustie, Andreas Schinkovitz, K Kerdia, N Jaozara, Thomas Guillemette, Philippe Simoneau, Pascal Richomme, P Le Pogam-Alluard, Substances d'Origine Naturelle et Analogues Structuraux (SONAS), Université d'Angers (UA), Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA)-Université d'Angers (UA), Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Chimie de Rennes-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), and Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
0106 biological sciences ,Pharmacology ,Alternaria brassicicola ,biology ,Organic Chemistry ,Brassica ,Usnic acid ,Pharmaceutical Science ,Lasallia pustulata ,biology.organism_classification ,Alternaria ,01 natural sciences ,Lobaria scrobiculata ,Analytical Chemistry ,010602 entomology ,chemistry.chemical_compound ,Complementary and alternative medicine ,chemistry ,Drug Discovery ,Botany ,[CHIM]Chemical Sciences ,Molecular Medicine ,Lichen ,010606 plant biology & botany ,Black spot - Abstract
International audience; Alternaria brassicicola (Pleosporaceae) is destructive plant fungus causing black spot disease on virtually every important cultivated Brassica species including broccoli, cabbage, canola, and mustard [1,2. The pathogen is responsible for significant economic damage as infections may occasionally lead to 20 – 50% yield reductions in crops such as canola, mustard and rape. [2]. Furthermore A. brassicicola is a potent source of mold-derived allergens inducing chronic respiratory diseases such as asthma and chronic rhinosinusitis in humans. Lichens are complex symbiotic organisms consisting of fungi and cyanobacteria or algae. They are known to produce a large variety of unique secondary metabolites some exhibiting notable anti-inflammatory, antioxidant, antibacterial but also antifungal activities [3]. Despite the wide range of studied biological activities found in lichens, very little is known about their effect on A. brassicicola. The present study therefore evaluated the antifungal activity of lichen extracts and compounds on A. brassicicola using a nephelometry based method for measuring fungal growth [1]. Crude extracts of Lasallia pustulata (Umbilicariaceae), Ramalina fastigiata (Ramalinaceae), Evernia prunastri (Parmeliaceae) and Lobaria scrobiculata (Lobariaceae) were found to exhibit interesting inhibitory activities at 68.3, 42.5, 40.0 and 34.7 percent inhibition at 25 µg/Ml, respectively. Furthermore (+) usnic acid (1) (57.5% at 72.67µM) and m-scrobiculin (2) (32.5% at 59.8µM) showed notable antifungal effects.
- Published
- 2016
- Full Text
- View/download PDF