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Choosing the right device for blood collection is an issue that is frequently encountered in laboratory medicine. The purpose of this study was to compare the quality of the samples collected in terms of hemolysis index, pain intensity and tube filling rates of the samples drawn with BD Vacutainer® EclipseNeedle and BD Vacutainer Ultra-Touch™ Butterfly Blood Collection Set, which is currently used in laboratories.

Aim: Psoriasis is a disease characterized by chronic inflammation of the skin and small joints that causes serious deterioration in quality of life. Inflammation, angiogenesis and fibrosis plays a significant role in the disease pathogenesis. Fibroblast growth factor 2 (FGF2) is a vigorous mitogenic agent and a molecule associated with pathways that are effective in the pathogenesis of psoriasis. The purpose of this study is to determine the level of patients with FGF2 in the serum of psoriasis and to determine the change in the level of this molecule according to the severity of the disease. Materials and Methods: 96 patients with psoriasis and 100 healthful were included in this study. The level of FGF2 was measured by ELISA method from serum samples obtained from blood samples taken from those included in the study. The results were compared with the healthful control group. Patients with psoriasis were grouped as mild, moderate, severe and very severe. FGF2 levels were compared in patients grouped according to disease severity. Results: Serum FGF2 levels were found to be higher in psoriasis patients compared to the healthful control group (p<0.0001). When psoriasis patients were checked against the healthy control group according to the severity of the disease, serum FGF2 levels were found to increase in the moderate, severe and very severe patient groups (p<0.001). Conclusion: Elevated serum FGF2 level in psoriasis patients was revealed for the first time. As the severity of the disease increases, the increase in the serum FGF2 level shows that this molecule may be effective in the pathogenesis of psoriasis and may be the target molecule in treatment. [ABSTRACT FROM AUTHOR]

The purpose of our study was to investigated the anti-Yo, anti-Hu, anti-Ri, anti-amphiphysin antibody levels and 8-OHdG in mothers of children with autism. This study included 60 participants, 33 of whom were healthy mothers of 3–12-year-old children diagnosed with autism spectrum disorder (ASD) and the 27 others who constituted the control group, were healthy mothers with age-matched healthy children. Two groups were examined for plasma anti-Yo, anti-Hu, anti-amphiphysin and anti-Ri antibodies and, 8-OHdG levels. The participants were asked to accomplish a sociodemographic data form. The severity of ASD symptoms was evaluated according to the Childhood Autism Rating Scale (CARS). Anti-amphiphysin antibody levels and anti-Ri antibody positivity were significantly higher in the case group (p = 0.001; p = 0.027, respectively). The two groups did not significantly differ in terms of anti-Yo and anti-Hu antibody levels and in terms of 8-OHdG levels (p = 0.065; p = 0.099; p = 0.490, respectively). The two groups did not significantly differ in terms of sociodemographic data (p > 0.05). According to the our study, maternal antineuronal antibodies, such as anti-amphiphysin and anti-Ri, may contribute to the risk of childhood autism. Studies with larger samples are needed. Maternal factors associated with autism should be investigated in order to create early diagnosis and treatment opportunities for autism. Based on the importance of immunological and cerebellar pathologies in autism aetiology, we aimed to investigate antineuronal antibodies in mothers of children with autism. Maternal antineuronal antibodies, such as anti-amphiphysin and anti-Ri, may contribute to the risk of childhood autism. High anti-amphiphysin antibody levels in mothers of children with autism may also occur against the amphiphysin in the structure of the SrGAP3 gene, which is associated with autism. [ABSTRACT FROM AUTHOR]

Choosing the right device for blood collection is an issue that is frequently encountered in laboratory medicine. The purpose of this study was to compare the quality of the samples collected in terms of hemolysis index, pain intensity and tube filling rates of the samples drawn with BD Vacutainer® EclipseNeedle and BD Vacutainer Ultra-Touch™ Butterfly Blood Collection Set, which is currently used in laboratories. Blood samples were drawn from the 44 apparently healthy adult individuals from different arm sites by routine phlebotomy into Citrate, SST and EDTA tubes in Karapınar State Hospital. K, P, AST, LDH tests and hemolysis index were analyzed by Mindray BS-800 chemistry analyzer. Complete blood count (CBC) was determined by Mindray BC 6000. There was no significant difference in K, P, AST, LDH and CBC values between the devices for hemolysis (p>0.05). No significant difference was found out with respect to visual hemolysis and hemolysis index, tube filling speed and pain intensity between the devices. Our findings demonstrate that two sets may used for venipuncture blood collection without creating additional hemolysis risk. [ABSTRACT FROM AUTHOR]

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Objective: The aim of this study was to investigate the maternal serum concentrations of copper (Cu) and ceruloplasmin (CP) in patients with mild preeclampsia, severe preeclampsia, hemolysis, elevated liver enzymes, and low platelet count (HELLP) syndrome, and to determine their association with the severity of the disease.Methods: This study was carried out at the largest tertiary care health center in the southeast region in Turkey and Department of Obstetrics and Gynecology, Dicle University Hospital. A total of 179 pregnant women, including 58 healthy pregnant women and 71 mild preeclampsia, 26 severe preeclampsia, and 24 HELLP syndrome cases classified according to the American College of Obstetricians and Gynecologists' 2013 guidelines were included in this prospective study. Blood samples were taken from all the pregnant women to evaluate the serum Cu and CP levels. The Cu level was determined via atomic absorption/emission spectroscopy, while the serum CP level was assessed with a nephelometric assay using an automatic image analyzer. Spearman's rank correlation tests were used to determine the correlations between the serum levels of the antioxidative markers and the preeclampsia severity.Results: The mean ± SD of the Cu was 81.2 ± 11.84 µg/dl in the mild preeclampsia cases and 160.2 ± 20.89 µg/dl in the severe preeclampsia cases (p < .001). The mean ± SD of the CP was 33.0 ± 4.81 mg/dl in the mild preeclampsia cases and 65.3 ± 9.17 mg/dl in the severe preeclampsia cases (p < .001). The Cu and CP levels were significantly higher in the patients with HELLP syndrome, which is an advanced and more severe form of severe preeclampsia, than in the mild and severe preeclampsia patients (p < .001 and p < .001, respectively). Therefore, the serum Cu and CP levels were correlated with the severity of preeclampsia (r = 859, p < .001 and r = 786, p < .001, respectively). In addition, there was a positive correlation between the serum Cu and CP levels and the systolic and diastolic blood pressure values and aspartate amino transferase levels (AST), and a negative correlation between the serum Cu and CP levels and the platelet count.Conclusion: This was the first study in which the ceruloplasmin and Cu levels were investigated in HELLP syndrome patients. When considering the results obtained in the present study, there were significant relationships between the Cu, CP levels which are the markers of oxidative stress and the preeclampsia severity. [ABSTRACT FROM AUTHOR]

Objective: The study aims to evaluate the maternal serum and the vaginal fluid levels of soluble vascular cell adhesion molecule-1 (sVCAM-1) and soluble intercellular adhesion molecular (sICAM-1) in pregnant women complicated by preterm prelabour ruptures of membranes (PPROM).Materials and Methods: The prospective case control study included 34 pregnant women with PPROM and 34 healthy pregnant women. Patients with additional diseases, a smoking habit and vaginal bleeding, as well as those using antibiotics, during the study period were not included in the study. Cervicovaginal fluid and serum samples were taken during the patients' admission. The demographic data, maternal serum and vaginal fluid sVCAM-1 and sICAM-1, C reactive protein (CRP) and leukocyte counts were noted for all pregnant women included in the study. The sVCAM-1 and sICAM-1 levels were measured by enzyme-linked immunosorbent assay kits.Results: In pregnant women with PPROM, the serum leukocyte (mean ± SD =11.41 ± 1.067 versus 9.18 ± 1.56, p < .0001), serum sVCAM-1 (median 771.20 versus 704.60 ng/ml, p < .001), sICAM-1 (mean ± SD 213.10 ± 35.59 ng/ml versus 188.11 ± 37.35 ng/ml, p = .06), vaginal sVCAM-1 (median 208.00 versus 140.20 ng/ml, p = .014) and sICAM-1 (mean ± SD 32.32 ± 6.49 ng/ml versus 24.87 ± 6.79 ng/ml, p < .001) values were found to be significantly higher in pregnant women with PPROM than in healthy pregnant women. A positive and significant correlation was observed between the leukocyte count and the vaginal sVCAM-1 level (r = 0.850; p < .001).Conclusion: To the best of our knowledge, this is the first study evaluating the levels of sICAM-1 in maternal serum in pregnant women with PPROM. The maternal serum and vaginal fluid sVCAM-1 and sICAM-1 levels can be used as biochemical markers supporting the PPROM diagnosis because of the increase in both maternal serum and vaginal fluid sVCAM-1 and sICAM-1 levels in pregnant women with PPROM. [ABSTRACT FROM AUTHOR]