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9 results on '"Kübler, Alexander Christian"'

1. Metabolic Silencing via Methionine-Based Amino Acid Restriction in Head and Neck Cancer

Author

Wünsch, Anna Chiara, primary, Ries, Elena, additional, Heinzelmann, Sina, additional, Frabschka, Andrea, additional, Wagner, Peter Christoph, additional, Rauch, Theresa, additional, Koderer, Corinna, additional, El-Mesery, Mohamed, additional, Volland, Julian Manuel, additional, Kübler, Alexander Christian, additional, Hartmann, Stefan, additional, and Seher, Axel, additional

Published
2023
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3. Mass Spectrometric Metabolic Fingerprinting of 2-Deoxy-D-Glucose (2-DG)-Induced Inhibition of Glycolysis and Comparative Analysis of Methionine Restriction versus Glucose Restriction under Perfusion Culture in the Murine L929 Model System

Author

Volland, Julian Manuel, primary, Kaupp, Johannes, additional, Schmitz, Werner, additional, Wünsch, Anna Chiara, additional, Balint, Julia, additional, Möllmann, Marc, additional, El-Mesery, Mohamed, additional, Frackmann, Kyra, additional, Peter, Leslie, additional, Hartmann, Stefan, additional, Kübler, Alexander Christian, additional, and Seher, Axel, additional

Published
2022
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6. Metabolic Fingerprinting of Murine L929 Fibroblasts as a Cell-Based Tumour Suppressor Model System for Methionine Restriction

Author

Schmitz, Werner, Koderer, Corinna, El-Mesery, Mohamed, Gubik, Sebastian, Sampers, Rene, Straub, Anton, Kübler, Alexander Christian, and Seher, Axel

Subjects
liquid chromatography/mass spectrometry, methionine restriction, Cell Death, Tumor Necrosis Factor-alpha, L929, LC/MS, Fibroblasts, Ligands, Article, Cell Line, lcsh:Chemistry, Mice, Methionine, lcsh:Biology (General), lcsh:QD1-999, Metabolome, Animals, ddc:610, caloric restriction, lcsh:QH301-705.5, metabolism, amino acid, Cell Proliferation, mass spectrometry
Abstract

Since Otto Warburg reported in 1924 that cancer cells address their increased energy requirement through a massive intake of glucose, the cellular energy level has offered a therapeutic anticancer strategy. Methionine restriction (MetR) is one of the most effective approaches for inducing low-energy metabolism (LEM) due to the central position in metabolism of this amino acid. However, no simple in vitro system for the rapid analysis of MetR is currently available, and this study establishes the murine cell line L929 as such a model system. L929 cells react rapidly and efficiently to MetR, and the analysis of more than 150 different metabolites belonging to different classes (amino acids, urea and tricarboxylic acid cycle (TCA) cycles, carbohydrates, etc.) by liquid chromatography/mass spectrometry (LC/MS) defines a metabolic fingerprint and enables the identification of specific metabolites representing normal or MetR conditions. The system facilitates the rapid and efficient testing of potential cancer therapeutic metabolic targets. To date, MS studies of MetR have been performed using organisms and yeast, and the current LC/MS analysis of the intra- and extracellular metabolites in the murine cell line L929 over a period of 5 days thus provides new insights into the effects of MetR at the cellular metabolic level.

Published
2021
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8. Utilizing BMP-2 muteins for treatment of multiple myeloma

Author

Seher, Axel, primary, Lagler, Charlotte, additional, Stühmer, Thorsten, additional, Müller-Richter, Urs Dietmar Achim, additional, Kübler, Alexander Christian, additional, Sebald, Walter, additional, Müller, Thomas Dieter, additional, and Nickel, Joachim, additional

Published
2017
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9. The Selection of NFκB Inhibitors to Block Inflammation and Induce Sensitisation to FasL-Induced Apoptosis in HNSCC Cell Lines Is Critical for Their Use as a Prospective Cancer Therapy.

Author

Scheurer, Mario Joachim Johannes, Müller-Richter, Urs Dietmar Achim, Kübler, Alexander Christian, Seher, Axel, Brands, Roman Camillus, Hartmann, Stefan, and El-Mesery, Mohamed

Subjects
INFLAMMATION, TUMORS, CANCER treatment, KERATINOCYTES, ENZYME inhibitors
Abstract

Inflammation is a central aspect of tumour biology and can contribute significantly to both the origination and progression of tumours. The NFκB pathway is one of the most important signal transduction pathways in inflammation and is, therefore, an excellent target for cancer therapy. In this work, we examined the influence of four NFκB inhibitors—Cortisol, MLN4924, QNZ and TPCA1—on proliferation, inflammation and sensitisation to apoptosis mediated by the death ligand FasL in the HNSCC cell lines PCI1, PCI9, PCI13, PCI52 and SCC25 and in the human dermal keratinocyte cell line HaCaT. We found that the selection of the inhibitor is critical to ensure that cells do not respond by inducing counteracting activities in the context of cancer therapy, e.g., the extreme IL-8 induction mediated by MLN4924 or FasL resistance mediated by Cortisol. However, TPCA1 was qualified by this in vitro study as an excellent therapeutic mediator in HNSCC by four positive qualities: (1) proliferation was inhibited at low μM-range concentrations; (2) TNFα-induced IL-8 secretion was blocked; (3) HNSCC cells were sensitized to TNFα-induced cell death; and (4) FasL-mediated apoptosis was not disrupted. [ABSTRACT FROM AUTHOR]

Published
2019
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