Search

Research in many forensic science fields commonly uses domestic pigs (Sus spp.) as proxies for human remains, due to their physiological and anatomical similarities, as well as being more readily available. Unfortunately, previous research, especially that which compares the decompositional process, has shown that pigs are not appropriate proxies for humans. To date, there has not been any published research that specifically addresses whether domestic pigs are adequate human proxies for the geophysical detection of clandestine graves. As such, the aim of this paper was to compare the geophysical responses of pig cadavers and human donor graves, in order to determine if pigs can indeed be used as adequate human proxies. To accomplish this, ground penetrating radar (GPR) and electrical resistivity tomography (ERT) responses on single and multiple pig cadaver graves were compared to single and multiple human donor graves, all of which are in known locations within the same geological environment. The results showed that under field conditions, both GPR and ERT were successful at observing human and pig burials, with no obvious differences between the detected geophysical responses. The results also showed that there were no differences in the geophysical responses of those who were clothed and unclothed. The similarity of the responses may reflect that the geophysical techniques can detect graves despite what their contents are. The study implications suggest that experimental studies in other soil and climate conditions can be easily replicated, benefiting law enforcement with missing persons cases., (© 2023 The Authors. Journal of Forensic Sciences published by Wiley Periodicals LLC on behalf of American Academy of Forensic Sciences.)

By nature, clandestine burials are difficult to locate, an issue that can complicate the legal process, and interrupt the natural grief process of the family. The purpose of this paper is to present a three-step process to search for clandestine graves using (1) geographic profiling, (2) light detection and ranging (LiDAR), and (3) near surface geophysics. Each process incrementally decreases the geographic area being searched, while increasing the level of detail provided to investigators. Using two well-known Australian cases and one experimental study, this paper will demonstrate how (1) can highlight potential search areas, (2) can further narrow down the location of potential burial sites within these search areas, and (3) can assist with locating the clandestine grave. Although each technique on its own can successfully locate graves, combining the techniques can provide the most efficient approach to locate those who are missing and buried., (© 2022 The Authors.)

Traditional biomass stoves are a major global contributor to emissions that impact climate change and health. This paper reports emission factors of particulate matter (PM 2.5 ), carbon monoxide (CO), organic carbon (OC), black carbon (EC), optical absorption, and scattering from 46 South Asian, 48 Tibetan, and 4 Ugandan stoves. These measurements plus a literature review provide insight into the robustness of emission factors used in emission inventories. Tibetan dung stoves produced high average PM 2.5 emission factors (23 and 43 gkg -1 for chimney and open stoves) with low average EC (0.3 and 0.7 gkg -1 , respectively). Comparatively, PM 2.5 from South Asian stoves (7 gkg -1 ) was in the range of previous measurements and near values used in inventories. EC emission factors varied between stoves and fuels ( p < 0.001), without corresponding differences in absorption; stoves that produced little EC, produced enough brown carbon to have about the same absorption as stoves with high EC emissions. In Tibetan dung stoves, for example, OC contributed over 20% of the absorption. Overall, EC emission factors were not correlated with PM 2.5 and were constrained to low values, relative to PM 2.5 , over a wide range of combustion conditions. The average measured EC emission factor (1 gkg -1 ), was near current inventory estimates.

DNA topoisomerase IIα (TOP2α) is a prominent target for anticancer drugs whose clinical efficacy is often limited by chemoresistance. Using antibody specific for the N-terminal of TOP2α, immunoassays indicated the existence of two TOP2α isoforms, 170 and 90 kDa, present in K562 leukemia cells and in an acquired etoposide (VP-16)-resistant clone (K/VP.5). TOP2α/90 expression was dramatically increased in etoposide-resistant K/VP.5 compared with parental K562 cells. We hypothesized that TOP2α/90 was the translation product of novel alternatively processed pre-mRNA, confirmed by 3'-rapid amplification of cDNA ends, polymerase chain reaction, and sequencing. TOP2α/90 mRNA includes retained intron 19, which harbors an in-frame stop codon, and two consensus poly(A) sites. The processed transcript is polyadenylated. TOP2α/90 mRNA encodes a 90,076-Da translation product missing the C-terminal 770 amino acids of TOP2α/170, replaced by 25 unique amino acids through translation of the exon 19/intron 19 read-through. Immunoassays, utilizing antisera raised against these unique amino acids, confirmed that TOP2α/90 is expressed in both cell types, with overexpression in K/VP.5 cells. Immunodetection of complex of enzyme-to-DNA and single-cell gel electrophoresis (Comet) assays demonstrated that K562 cells transfected with a TOP2α/90 expression plasmid exhibited reduced etoposide-mediated TOP2α-DNA covalent complexes and decreased etoposide-induced DNA damage, respectively, compared with similarly treated K562 cells transfected with empty vector. Because TOP2α/90 lacks the active site tyrosine (Tyr 805 ) of full-length TOP2α, these results strongly suggest that TOP2α/90 exhibits dominant-negative properties. Further studies are underway to characterize the mechanism(s) by which TOP2α/90 plays a role in acquired resistance to etoposide and other TOP2α targeting agents., (Copyright © 2016 by The American Society for Pharmacology and Experimental Therapeutics.)

Background: Adolescent depression is both common and burdensome, and while evidence-based strategies have been developed to prevent adolescent depression, participation in such interventions remains extremely low, with less than 3% of at-risk individuals participating. To promote participation in evidence-based preventive strategies, a rigorous marketing strategy is needed to translate research into practice., Objective: To develop and pilot a rigorous marketing strategy for engaging at-risk individuals with an Internet-based depression prevention intervention in primary care targeting key attitudes and beliefs., Method: A marketing design group was constituted to develop a marketing strategy based on the principles of targeting, positioning/competitor analysis, decision analysis, and promotion/distribution and incorporating contemporary models of behavior change. We evaluated the formative quality of the intervention and observed the fielding experience for prevention using a pilot study (observational) design., Results: The marketing plan focused on "resiliency building" rather than "depression intervention" and was relayed by office staff and the Internet site. Twelve practices successfully implemented the intervention and recruited a diverse sample of adolescents with > 30% of all those with positive screens and > 80% of those eligible after phone assessment enrolling in the study with a cost of $58 per enrollee. Adolescent motivation for depression prevention (1-10 scale) increased from a baseline mean value of 7.45 (SD = 2.05) to 8.07 poststudy (SD = 1.33) (P = .048)., Conclusions: Marketing strategies for preventive interventions for mental disorders can be developed and successfully introduced and marketed in primary care.

We describe the prototype to product development process of a low cost, socio-culturally relevant, easily implemented Internet-based depression prevention intervention for adolescents in primary care. The intervention named "Project CATCH-IT" (Competent Adulthood Transition with Cognitive-behavioral, Humanistic and Interpersonal Training) includes an initial motivational interview in primary care to engage the adolescent, fourteen Web-based modules based on behavioral activation, cognitive behavioral and interpersonal psychotherapy which target known risk factors, and a follow-up motivational interview in primary care. This was successfully fielded in a pilot study with 25 adolescents. We know of no other similar interventions developed for the prevention of depression in youth that is potentially universally available at low cost and that utilizes existing systems of healthcare providers.

OBJECTIVE: Risk factors for various disorders are known to cluster. However, the factor structure for behaviors and beliefs predicting depressive disorder in adolescents is not known. Knowledge of this structure can facilitate prevention planning. METHODS: We used the National Longitudinal Study of Adolescent Health (AddHealth) data set to conduct an exploratory factor analysis to identify clusters of behaviors/experiences predicting the onset of major depressive disorder (MDD) at 1-year follow-up (N=4,791). RESULTS: Four factors were identified: family/interpersonal relations, self-emancipation, avoidant problem solving/low self-worth, and religious activity. Strong family/interpersonal relations were the most significantly protective against depression at one year follow-up. Avoidant problem solving/low self-worth was not predictive of MDD on its own, but significantly amplified the risks associated with delinquency. CONCLUSION: Depression prevention interventions should consider giving family relationships a more central role in their efforts. Programs teaching problem solving skills may be most appropriate for reducing MDD risk in delinquent youth.

Introduction: There is currently no depression prevention intervention available to adolescents in primary care., Method: Fourteen late adolescents evaluated the acceptability (performance and ratings), potential adverse effects (negative emotions), and benefits (pre/post measures of three risk factors for depression) of a combined primary care Internet-based depression prevention intervention. Nearly all participants engaged the website (13/14) and 8/14 completed the entire intervention., Results: Completers reported high levels of readability and ease of understanding and low levels of negative emotions. Completers showed favorable trends for the targeted risk factors: depressive symptoms, dysfunctional thinking, and low social support., Conclusion: A primary care/prevention intervention may be acceptable and favorably impact risk factors for depression.