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This case study demonstrates the potential of the Cos4Cloud services FASTCAT-Edge and FASTCAT-Cloud to improve the camera trap community users’ experience. It is focused on a testing session that Cos4Cloud organised with the FELIS’ group, a camera trap community in Catalonia, Spain that uses camera traps to monitor Catalan mammals, focusing on the wildcat (Felis silvestris). This is one of the case studies demonstrating Cos4Cloud success stories and best practice created as part of the legacy and lessons learned from the project developed and shared by The Open University (OU), in collaboration with project partners, forthe Cos4Cloud Toolbox & Evidence Hub. Case studies editor: Janice Ansine, OU. Case studies designer: Harriett Cornish, OU. Contact: cos4cloud-toolbox@open.ac.uk&nbsp

D6.3 Citizen-science toolbox and evidence hub (renamed as the Cos4Cloud Toolbox and Evidence Hub) has been developed as a legacy collection of different materials and resources. It will be a ‘one stop shop’ of Cos4Cloud best practice demonstrating project results, this is being collated within the following category themes: Training & capacity building resources: Cos4Cloud co-designed technological services Best practice guidelines & resources for COs: Citizen observatories working collaboratively Educational resources: Examples of citizen science school-based approaches Case studies: demonstrating Cos4Cloud success stories and best practice examples Evidence Hub: feedback, discussion threads / themes, participation and engagement activities The development of the Cos4Cloud Toolbox and Evidence Hub contributes to the delivery of Work Package 6 (WP6) Networking, (education), training and capacity building, led by the OU. This output contributed to creating and sharing best practice guidelines, training, capacity building and educational citizen science resources. The Cos4Cloud Toolbox & Evidence Hub was envisioned as a ‘one-stop-shop’ of Cos4Cloud best practice guidelines and materials for existing and future citizen observatory leaders incorporating case studies, training, capacity building and educational citizen science resources. The main goal of WP6 is to demonstrate new conceptual models for evidence-based knowledge exchange, capacity-building, best practice learning and engagement with and for citizen science, focusing on citizen observatories.

This is a system and user guide to MECODA, an online service that provides a repository of tools that facilitate analysis and visualisation of citizen science data from citizen observatories (COs). MECODA (ModulE for Citizen Observatory Data Analysis) is one of the technological services developed in the Cos4Cloud project framework by Instituto de Ciencias del Mar (ICM-CSIC). More information:https://cos4cloud-eosc.eu/services/mecoda-data-analysis-package/ This system and user guide is one of the Cos4Cloud Training and Capacity Building Resourcesdeveloped by The Open University (OU), in collaboration with project partners, forthe Cos4Cloud Toolbox & Evidence Hub. System and user guide designer: Harriett Cornish, OU. Contact: cos4cloud-toolbox@open.ac.uk &nbsp

This Knowledge Transfer Report describes and evaluate Knowledge Transfer activities undertaken by the Cos4Cloud project. Cos4Cloud's methodology to develop and monitor its KT strategy was based on common practices implemented by the H2020 community and on requirements and recommendations from the funding agency, the European Commission. It is important to underline that some aspects of a KT strategy cannot be evaluated quantitatively and have been challenging to measure. Having said that, the Cos4Cloud methodology for KT transfer and its evaluation has proven itself effective for keeping track of the consortium efforts in KT, monitoring the Cos4Cloud KT activities and whether they were aligned with the target stakeholders and the KT goals, as well as to adjust the strategy if necessary. Implementing and evaluating the Cos4Cloud Knowledge Transfer framework has been a unique opportunity to understand KT within the project, providing us with some practical lessons to make this transfer effective. In the case of Cos4Cloud, setting up clear KT Outputs and key stakeholders was crucial for developing the project’s KT strategy and involving all the consortium members in its implementation. Besides, in Cos4Cloud, the events were key for the success of the KT strategy; they allowed us to be in direct contact with the community and make this transfer more effective.

This report analyses the engagement strategy designed and implemented in the Cos4Cloud project, as well as its evaluation.&nbsp

This presentation summarises the results obtained during the event 'Co-designing solutions for the sustainability and governance of citizen observatories' at the ECSA'sConference 2022. Around 25 participants attended the workshop and shared ideas for improving citizen observatories' sustainability and governance. The presentation is divided into two main sections: (1) the workshop's context, the co-design methodology implemented, (2) and the main results. The activity is part of the Cos4Cloud co-design strategy.&nbsp

This infographic summarises the results of thefirst international editionof the BioMARató-Beaches with life, a friendly marine citizen science competition between Barcelona Metropolitan Area (Spain) and Tremiti Island (Italy). BioMARatóis an activity organized by the ICM-CSIC using the Citizen Observatory MINKA (developed by the ICM-CSIC). The entities responsible for dinamizing the snorkelling and diving activities areAnèl-lides - Serveis ambientals marins, the Catalan Federation of Underwater Activities (FECDAS),Xatrac,Plàncton Diving, Marlintremiti: Laboratorio del Mareand Cluster Biodiversità Italia. In addition, the European Horizon2020 projectsCos4CloudandMINKE, coordinated by the ICM-CSIC, the diving centresiDive,Saita Diving,Vanas Dive,Underwater BarcelonaandOCEANICOS.

This infographic summarises the results of the second edition of the BioMARató-Beaches with life, one of the most significant citizen science and marine observations events in Catalonia. BioMARatóis an activity promoted by the ICM-CSIC. The entities responsible for organising the activities areAnèl-lides - Serveis ambientals marins, the Catalan Federation of Underwater Activities (FECDAS),XatracandPlàncton Diving. In addition, the European Horizon2020 projectsCos4CloudandMINKE, coordinated by the ICM-CSIC, the diving centresiDive,Saita Diving,Vanas Dive,Underwater BarcelonaandOCEANICOS.

Aquesta infografia resumeix els resultats de la segona edició de laBioMARató-Platgesamb vida, un dels majors esdeveniments de ciència ciutadana i observacions marines a Catalunya. LaBioMARatóés una activitat impulsada per l’ICM-CSIC. Les entitats encarregades de dinamitzar les activitats sónAnèl·lides – Serveis ambientals marinsla Federació Catalana d'Activitats Subaquàtiques (FECDAS), Xatrac i Plàncton Diving. A més, col·laboren els projectes europeus Horizon2020Cos4CloudiMINKE, coordinats per l’ICM-CSIC, els centres de busseigiDive,Saita Diving,Vanas Dive,Underwater BarcelonaiOCEANICOS.

Esta infografía resume los resultados de lasegunda edición de laBioMARató-Playas con vida, uno de los mayores acontecimientos de ciencia ciudadana y observaciones marinas en Cataluña. LaBioMARatóes una actividad impulsada por el ICM-CSIC. Las entidades encargadas de dinamizar las actividades sonAnèl·lides – Serveis ambientals marins, la Federación Catalana de Actividades Subacuáticas (FECDAS),XatracyPlàncton Diving. Además, colaboran los proyectos europeos Horizon2020Cos4CloudyMINKE, coordinados por el ICM-CSIC, los centros de buceoiDive,Saita Diving,Vanas Dive,Underwater BarcelonayOCEANICOS.

This guide results from the experience and lessons learned in co-designing thirteen technologicalservices within the Cos4Cloud project. The technological services were created from a bottom-up approach, following a co-design process connected to an agile software development methodology. The co-design team implemented the co-design process as a service within the Cos4Cloud consortium, as stated in task 5.2. The result of implementing the co-design framework and methodologies and the corresponding monitoring during the co-design of the services, were evaluated and adapted to be shared with the citizen science community as a methodological guide. The present methodological guide includes an introduction to the world of co-design, where the main definitions and mindsets of co-design and design thinking are given, as well as some tools to start applying the creative methodologies within them. Secondly, it introduces the co-design methodology followed in Cos4Cloud, together with some important concepts, such as citizen science and citizen observatories. Then, a practical guide of implementation is given, including all the co-design methodologies used in Cos4Cloud. The most standard methods include a description and a work map template, while the customised ones incorporate a step-by-step guide to implement them. Finally, an explanation is given on how the co-design process was implemented in Cos4Cloud as a service for software developers, by using three interactive platforms that allowed us to perform all creative sessions. The document concludes with an invitation to implement co-design processes in other software development projects.

Prolonged cold stress has a strong effect on plant growth and development, especially in subtropical crops such as maize. Soil temperature limits primary root elongation, mainly during early seedling establishment. However, little is known about how moderate temperature fluctuations affect root growth at the molecular and physiological levels. We have studied root tips of young maize seedlings grown hydroponically at 30 ºC and after a short period (up to 24 h) of moderate cooling (20 ºC). We found that both cell division and cell elongation in the root apical meristem are affected by temperature. Time-course analyses of hormonal and transcriptomic profiles were achieved after temperature reduction from 30 ºC to 20 ºC. Our results highlighted a complex regulation of endogenous pathways leading to adaptive root responses to moderate cooling conditions.

This story derives from a market need identified by the service provider. There are thousands of applications in the natural sciences field who were asking for an automated plant identification system within their own applications. The proposed solution is called Pl@ntNET, a citizen observatory and AI-based Platform designed to monitor plants biodiversity and help identify plants via pictures. It is organised in different thematic and geographical floras operational via a search engine.The solution is provided by a consortium of French research organisms piloted by Inria, the French national research institute for digital science and technology. The user community of Pl@ntNet consists of (1) the citizen and citizen scientists who are interested in identifying and knowing more about plants, and (2) developers of applications and software for plant identification, gardening, biodiversity management, agroecology or other related fields. The service is available on theEOSC Portal Catalogue and Marketplaceand was developed within theCos4Cloud Project. The full list of EOSC in practice stories is available here

El documento relata qué es el co-diseño ycómo se aplica en proyectos de ciencia ciudadana. Lo hace a través de un caso de éxito el proyecto Cos4Cloud*. *Cos4Cloud es un proyecto europeo coordinadoporelICM-CSICquecuentacon 15 partners devariospaísesde Europa y Colombia.Elproyecto ha recibido financiación del programa de investigación e innovación Horizonte 2020 de la Unión Europea en virtud del acuerdo de subvención nº 863463.&nbsp

This presentation explains what co-design is, why it is useful and how to apply it in citizen science in general and in creating technological citizen science services in particular to explain it, we the presentation uses the Cos4Cloud* experience. *Cos4Cloud is a project coordinated by the ICM-CSIC and funded by Horizon2020, which has 15 partners in several European countries and Colombia. Ithas received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement no 863463.

This EOSC in practice storywas developed within theCos4cloud projectandtargets a very wide user base as it is addressed to any researchers, teachers, students, companies, institutions and, more generally, anyone interested in knowing, studying or analysing biodiversity information. The story presents Cos4Bio,a co-designed, interoperable and open-source service that integrates biodiversity observations from multiple citizen observatories in one place, allowing experts to save time in the speciesidentification process and get access to an enormous number of biodiversity observations. This resource is available on theEOSC Portal Catalogue and Marketplace The full list of EOSC in practice stories is available here

ThisEOSC in practice storytargets three main types of users: (1) the citizen and citizen scientists who collect valuable data, (2) the researchers who use and benefit from the data collected by citizens via smartphones and sensors, and (3) software developers. The service presented is calledMOBIS,developed byDDQ: Pocket Science, a small Dutch company specialised in the development of mobile-based (citizen-) science applications with research partners worldwide. Such solution aims at solving the problem ofhigh fragmentationin the citizen science apps ecosystem offering anintegration service. The service is available on theEOSC Portal Catalogue and Marketplaceand was developed within theCos4Cloud Project. The full list of EOSC in practice stories is available here

PurposeCentral venous catheters (CVCs) have become common practice in oncology. Besides their benefits, as an invasive procedure, several complications are associated with them. Catheter associated thrombosis (CAT) is one of the most relevant due to their impact in quality of life and mortality, but the prothrombotic risk factors implied have been poorly assessed. The aim of the study is to evaluate the incidence of upper extremity deep vein thrombosis (UEDVT) associated to catheter use in patients with solid tumors. Secondary endpoints are to describe the population using CVCs and to evaluate potential risk factors of CAT. MethodsPatients diagnosed of solid tumors assisted at a tertiary level hospital between 2016 and 2019, and using CVCs were included.Results455 patients were enrolled. The incidence of CAT was 5.49% (25) in the whole population. 5.05% (23) was associated with PICC while 0.44% (2) was due to PORT use. Among the factors included in the univariate and multivariate regression models, age ≥50 years and PORT use were identified as protective factors related to the development of CAT.ConclusionCVCs remains a safe approach for the delivering of treatments in patients with solid tumors. Age ≤50 and use of PICC are risk factors for developing CAT. Larger prospective studies are needed to identify additional risk factors of CAT.

These are the slides from the LIBER 2021 Workshop: Onboarding Citizen Science and the role of research libraries barriers and accelerators This workshop derives from the SSHOC - Social Sciences and Humanities Open Cloud work in collaboration with the LIBER Citizen Science working group. It will be building on the outcomes of the session“ Citizen Science: What it means for SSH and how can multidisciplinarity be achieved?” that took place during the conference “Realising the European Open Science Cloud: Towards a FAIR research data landscape for the social sciences, humanities and beyond.” in November 2020. Objective The overarching aim of this workshop is to raise the awareness of the challenges and opportunities in libraries’ involvement with Citizen Science in SSH. It aims to explore the extent to which research libraries can serve as a bridge between researchers and citizens. An approach based on the sharing of best practices and current research, the involvement of participants in the reflection of the respective roles and training needed, complemented by an open discussion, will shape the overall experience. One of the outputs of this workshop will be the production of actionable recommendations for research libraries and the SSHOC/SSH communities in the form of a report. This will be complemented by a proposed outline of potential awareness raising and training sessions (for librarians, researchers, and citizen scientists) that would help participants in implementing those recommendations. This workshop will be an opportunity to create further synergies between LIBER, SSHOC and initiatives represented by the speakers and the participants.

Introducción: El trasplante de órganos es, en casos de lesión irreversible de algún órgano y pérdida irrecuperable de su función, la única alternativa para poder recuperar la salud. Sin embargo, el principal factor limitante a esta terapia es la escasez de órganos. Para disminuir las negativas familiares a la donación de órganos, fuertemente influenciadas por diferentes factores psicosociales, debemos investigar sobre la actitud y el conocimiento de las variables que influyen en la donación, especialmente en grupos sociales con actitud más negativa, como la etnia gitana. El objetivo de esta tesis es conocer la actitud hacia la donación de órganos propios para el trasplante tras el fallecimiento entre la población gitana española mayor o igual a 15 años y analizar las variables psicosociales que condicionan dicha actitud. Material y método: Estudio observacional, transversal, de ámbito nacional, español en población gitana con edad igual o mayor de 15 años, estratificada por edad y género. Se estimó un tamaño muestral de 1253. Como instrumento de medida se emplea un cuestionario validado de actitud hacia la donación (“PCID–DTO Ríos”: Cuestionario del Proyecto Colaborativo Internacional Donante). Para el análisis estadístico se utilizó el test de Student, el test de chi-cuadrado y un análisis de regresión logística. Resultados: El cuestionario fue cumplimentado por 228 de 1253 encuestados, que implica un 18,2% de grado de cumplimentación y 81,8% de pérdidas. Se objetiva que la opinión hacia la donación de órganos propios tras el fallecimiento es favorable en 42,1% (n=96), estando el 27,6% (n=63) en contra y el 30,3% de indecisos (n=69). Debido a la baja cumplimentación, uno de los objetivos planteados era conocer las razones de dicha negativa. El 97,9% de los que no cumplimentan, argumentan miedo a hablar del tema de la muerte y la donación y si consideramos que éstos no presentan una actitud favorable hacia la donación de órganos propios, los resultados serían los siguientes: El 7,7% (n=96) presentan una actitud favorable, frente al 90,7% (n=1136) que presentan una actitud no favorable. Edad media 35,4 ± 14,4 años. Las siguientes variables se asociaron con la actitud hacia la donación: edad (p=0.029), género (p=0,018), nivel de estudios del encuestado (p

This deliverable, produced within Work Package 6 (WP6) Task 6.1, identifies 78 citizen science (CS), citizen observatory (CO) projects, CO portals and other initiatives of interest (past, current and future) that may either contribute to or make use of the Cos4Cloud platform and services. The goal was to identify a group of projects and initiatives that we might productively work with across the duration of Cos4Cloud, not to produce a comprehensive list of projects.

The adventitious root formation is critical for the plant survival during its vegetative propagation through cuttings, hence the study and understanding of this process is strategic during mass propagation of the new elite varieties obtained by genetic breeding. This Thesis pursues genetic, morphological and hormonal studies of de novo root formation in the Arabidopsis thaliana (L.) Heynh. model, and in two other plant species of agronomic interest in the Mediterranean region, such as the cultivated carnation (Dianthus caryophyllus L.) and the argan tree (Argania spinosa [L.] Skeels). To identify some genetic determinants involved in woundinduced root formation process, we carried out a genomewide association (GWA) study in a collection of 174 Arabidopsis thaliana natural accessions. We found statistically significant associations between woundinduced root system architectural traits and 19 genomic loci. We then studied three candidate loci associated with woundinduced lateral root formation. Lossoffunction mutants in one of these genes, At4g01090, which encodes a protein of unknown function, were affected in woundinduced lateral root development. We identified null alleles in the At5g19710 gene, which encodes a histidine phosphotransfer protein putatively involved in cytokinin signalling, with a reduced number of lateral roots after root tip excision. In addition, we found an epistatic interaction between several nonsynonymous single nucleotide polymorphisms in the coding region of K+ UPTAKE TRANSPORTER 5 (KUP5), which encodes a potassium uptake transporter; and At5g19710, which would point to a functional relationship between both processes and woundinduced root formation. We studied morphological changes occurring in the basal region of stem cuttings during rooting in a collection of 159 F1 lines derived from a cross between two carnation hybrid cultivars, 210102 MFR and 2003 R 8, with contrasting rooting performances. We provided molecular evidence that the badrooting behaviour of 2003 R 8 cuttings was caused by an early inactivation of active auxin, indole3acetic acid, through its conjugation with aspartic acid by GRETCHEN HAGEN 3 proteins. Our analyses further elucidated the relationship between the physiological state of carnation cuttings, estimated by the proportion of salicylic acid and jasmonate, and the formation of adventitious roots. In addition, we developed an effective method for argan micropropagation from in vitro germinated seeds and we considerably increased the adventitious rooting from lignified cuttings obtained from adult plants. When combined with a suitable genetic selection, our procedure will allow the efficient production of elite varieties of argan trees for intensive cultivation. La formación de raíces adventicias supone un paso crítico y de vital importancia para la supervivencia de las plantas que se propagan vegetativamente mediante esquejes, por lo que el estudio y la comprensión de este proceso es clave para optimizar la propagación vegetativa de las nuevas variedades élite obtenidas por métodos convencionales de mejora genética. En esta Tesis se propone el estudio a nivel genético, morfológico y hormonal de la formación de raíces de novo en la crucífera modelo Arabidopsis thaliana (L.) Heynh., y en dos especies de interés agronómico en la región mediterránea: el clavel cultivado (Dianthus caryophyllus L.) y el argán (Argania spinosa [L.] Skeels). Para identificar algunos de los determinantes genéticos implicados en la formación de raíces en respuesta a herida, hemos llevado a cabo un estudio de asociación a genoma completo o GWA (genomewide association study) en una colección de 174 estirpes silvestres de Arabidopsis thaliana. Nuestros resultados indican que existen asociaciones estadísticamente significativas entre los cambios en la arquitectura radicular en respuesta a herida y 19 regiones genómicas concretas. Nos hemos centrado en el análisis genético de 6 polimorfismos de un solo nucleótido o SNPs (single nucleotide polymorphisms) que generan cambios no conservados de residuos aminoacídicos en la región codificante de tres genes. Los mutantes de pérdida de función en At4g01090, que codifica una proteína de función desconocida, muestran un menor número de raíces laterales en respuesta a herida. Hemos identificado alelos nulos en el gen At5g19710, que codifica una proteína con un dominio de histidina para la fosfotransferencia implicado en la señalización de las citoquininas, con un número reducido de raíces laterales en respuesta a herida. Además, hemos encontrado una interacción epistática entre los SNPs de la región codificante de K+ UPTAKE TRANSPORTER 5 (KUP5), cuyo producto participa en la asimilación del potasio, y At5g19710, lo que apuntaría a una relación funcional entre ambos procesos y con la formación de raíces en respuesta a herida. Por otra parte, hemos llevado a cabo un análisis detallado de la formación de raíces adventicias a partir de esquejes de clavel cultivado en una colección de 159 líneas F1 derivadas del cruzamiento entre dos variedades comerciales, 210102 MFR y 2003 R 8, que difieren de forma significativa en su capacidad de enraizamiento adventicio. Hemos proporcionado evidencias moleculares de que la baja capacidad de enraizamiento adventicio de los esquejes en la estirpe 2003 R 8 se debe a una inactivación temprana de la auxina activa, el ácido indolacético, en la base del esqueje mediante su conjugación con ácido aspártico por enzimas de la familia GRETCHEN HAGEN 3. Nuestros análisis han permitido dilucidar, además, la relación existente entre el estado fisiológico de los esquejes de clavel, estimada por la proporción de ácidos salicílico y jasmónico, y la formación de raíces adventicias. Adicionalmente, hemos desarrollado un método eficaz para la micropropagación del argán a partir de semillas germinadas in vitro, además de un protocolo que ha permitido incrementar considerablemente la formación de raíces adventicias a partir de esquejes lignificados obtenidos de plantas adultas. Combinado con una selección genética adecuada, nuestro procedimiento permitirá la producción eficiente de variedades élite de argán para su uso en cultivo intensivo.

Plants have a remarkable regenerative capacity, which allows them to survive tissue damage after biotic and abiotic stresses. In this study, we use Solanum lycopersicum ‘Micro-Tom’ explants as a model to investigate wound-induced de novo organ formation, as these explants can regenerate the missing structures without the exogenous application of plant hormones. Here, we performed simultaneous targeted profiling of 22 phytohormone-related metabolites during de novo organ formation and found that endogenous hormone levels dynamically changed after root and shoot excision, according to region-specific patterns. Our results indicate that a defined temporal window of high auxin-to-cytokinin accumulation in the basal region of the explants was required for adventitious root formation and that was dependent on a concerted regulation of polar auxin transport through the hypocotyl, of local induction of auxin biosynthesis, and of local inhibition of auxin degradation. In the apical region, though, a minimum of auxin-to-cytokinin ratio is established shortly after wounding both by decreasing active auxin levels and by draining auxin via its basipetal transport and internalization. Cross-validation with transcriptomic data highlighted the main hormonal gradients involved in wound-induced de novo organ formation in tomato hypocotyl explants.