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2 results on '"Jussara Cardozo"'

1. Identification and Genomic Characterization of a New Virus ( Tymoviridae Family) Associated with Citrus Sudden Death Disease

Author

Fernando C. Reinach, Hamilton Jordão, Jesus Aparecido Ferro, Giovana Camarotte, Alessandra Girasol, Moshe Bar-Joseph, Paulo Arruda, João Paulo Kitajima, Rachel F. Kamla, Vitor H. A. Silva, Christian C. Greggio, Leonardo C. A. Souza, Walter Maccheroni, P. F. Giachetto, Ana C. R. da Silva, Jussara Cardozo, Sulamita F. Franco, João Paulo Piazza, Almir S. Zanca, Elaine C. Casagrande, Elliot W. Kitajima, Paula R. A. Zacharias, C. I. Aguilar-Vildoso, Marcos C. Alegria, Laura C. Assumpção, and Fernanda Ferrari

Subjects
Citrus, food.ingredient, Marafivirus, viruses, Molecular Sequence Data, Immunology, Genome, Viral, Microbiology, Sudden death, Tymoviridae, Virus, food, Phylogenetics, Virology, Animals, Amino Acid Sequence, Phylogeny, Plant Diseases, Whole genome sequencing, Base Sequence, Phylogenetic tree, biology, cDNA library, Structure and Assembly, food and beverages, biology.organism_classification, Microscopy, Electron, Aphids, Insect Science, DNA, Viral, Capsid Proteins, Brazil
Abstract

Citrus sudden death (CSD) is a new disease that has killed approximately 1 million orange trees in Brazil. Here we report the identification of a new virus associated with the disease. RNAs isolated from CSD-affected and nonaffected trees were used to construct cDNA libraries. A set of viral sequences present exclusively in libraries of CSD-affected trees was used to obtain the complete genome sequence of the new virus. Phylogenetic analysis revealed that this virus is a new member of the genus Marafivirus . Antibodies raised against the putative viral coat proteins allowed detection of viral antigens of expected sizes in affected plants. Electron microscopy of purified virus confirmed the presence of typical isometric Marafivirus particles. The screening of 773 affected and nonaffected citrus trees for the presence of the virus showed a 99.7% correlation between disease symptoms and the presence of the virus. We also detected the virus in aphids feeding on affected trees. These results suggest that this virus is likely to be the causative agent of CSD. The virus was named Citrus sudden death-associated virus .

Published
2005

2. The Complete Nucleotide Sequence and Genomic Organization of Citrus Leprosis Associated Virus, Cytoplasmatic type (CiLV-C).

Author

Renata Pascon, João Kitajima, Michèle Breton, Laura Assumpção, Christian Greggio, Almir Zanca, Vagner Okura, Marcos Alegria, Maria Camargo, Giovana Silva, Jussara Cardozo, Marcelo Vallim, Sulamita Franco, Vitor Silva, Hamilton Junior, Fernanda Oliveira, Poliana Giachetto, Fernanda Ferrari, Carlos Aguilar-Vildoso, and Fabrício Franchiscini

Subjects
NUCLEOTIDE sequence, NUCLEOTIDE analysis, CITRUS, BREVIPALPUS
Abstract

The Citrus leprosis disease (CiL) is associated to a virus (CiLV) transmitted by Brevipalpus spp. mites (Acari: Tenuipalpidae). CiL is endemic in Brazil and its recently spreading to Central America represents a threat to citrus industry in the USA. Electron microscopy images show two forms of CiLV: a rare nuclear form, characterized by rod-shaped naked particle (CiLV-N) and a common cytoplasmic form (CiLV-C) associated with bacilliform-enveloped particle and cytoplasmic viroplasm. Due to this morphological feature, CiLV-C has been treated as Rhabdovirus-like. In this paper we present the complete nucleotide sequence and genomic organization of CiLV-C. It is a bipartite virus with sequence similarity to ssRNA positive plant virus. RNA1 encodes a putative replicase polyprotein and an ORF with no known function. RNA2 encodes 4 ORFs. pl5, p24 and p61 have no significant similarity to any known proteins and p32 encodes a protein with similarity to a viral movement protein. The CiLV-C sequences are associated with typical symptoms of CiL by RT-PCR. Phylogenetic analysis suggests that CiLV-C is probably a member of a new family of plant virus evolutionarily related to Tobamovirus. [ABSTRACT FROM AUTHOR]

Published
2006

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