Search

Your search keyword '"Juretzek, Thomas"' showing total 24 results
Start Over Author "Juretzek, Thomas"
24 results on '"Juretzek, Thomas"'

3. Identification of New Microfoci and Genetic Characterization of Tick-Borne Encephalitis Virus Isolates from Eastern Germany and Western Poland.

Author

Król, Nina, Chitimia-Dobler, Lidia, Dobler, Gerhard, Kiewra, Dorota, Czułowska, Aleksandra, Obiegala, Anna, Zajkowska, Joanna, Juretzek, Thomas, and Pfeffer, Martin

Subjects
TICK-borne encephalitis viruses, FLAVIVIRUSES, TICK-borne encephalitis, CASTOR bean tick, ANAPLASMA phagocytophilum, TICK-borne diseases, VIRUS diseases
Abstract

(1) Background: Tick-borne encephalitis (TBE) is the most important tick-borne viral disease in Eurasia, although effective vaccines are available. Caused by the tick-borne encephalitis virus (TBEV, syn. Orthoflavivirus encephalitidis), in Europe, it is transmitted by ticks like Ixodes ricinus and Dermacentor reticulatus. TBEV circulates in natural foci, making it endemic to specific regions, such as southern Germany and northeastern Poland. Our study aimed to identify new TBEV natural foci and genetically characterize strains in ticks in previously nonendemic areas in Eastern Germany and Western Poland. (2) Methods: Ticks were collected from vegetation in areas reported by TBE patients. After identification, ticks were tested for TBEV in pools of a maximum of 10 specimens using real-time RT-PCR. From the positive TBEV samples, E genes were sequenced. (3) Results: Among 8400 ticks from 19 sites, I. ricinus (n = 4784; 56.9%) was predominant, followed by D. reticulatus (n = 3506; 41.7%), Haemaphysalis concinna (n = 108; 1.3%), and I. frontalis (n = 2; <0.1%). TBEV was detected in 19 pools originating in six sites. The phylogenetic analyses revealed that TBEV strains from Germany and Poland clustered with other German strains, as well as those from Finland and Estonia. (4) Conclusions: Although there are still only a few cases are reported from these areas, people spending much time outdoors should consider TBE vaccination. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

14. Species-specific and pathotype-specific binding of bacteria to zymogen granule membrane glycoprotein 2 (GP2)

Author

Schierack, Peter, primary, Rödiger, Stefan, additional, Kolenda, Rafal, additional, Hiemann, Rico, additional, Berger, Enrico, additional, Grzymajło, Krzysztof, additional, Swidsinski, Alexander, additional, Juretzek, Thomas, additional, Meissner, Dirk, additional, Mydlak, Karsten, additional, Reinhold, Dirk, additional, Nolan, Lisa K, additional, and Roggenbuck, Dirk, additional

Published
2014
Full Text
View/download PDF

16. Foamy Virus Integration

Author

Juretzek, Thomas, primary, Holm, Teresa, additional, Gärtner, Kathleen, additional, Kanzler, Sylvia, additional, Lindemann, Dirk, additional, Herchenröder, Ottmar, additional, Picard-Maureau, Marcus, additional, Rammling, Matthias, additional, Heinkelein, Martin, additional, and Rethwilm, Axel, additional

Published
2004
Full Text
View/download PDF

17. Feline Foamy Virus Genome and Replication Strategy

Author

Roy, Jacqueline, primary, Rudolph, Wolfram, additional, Juretzek, Thomas, additional, Gärtner, Kathleen, additional, Bock, Michael, additional, Herchenröder, Ottmar, additional, Lindemann, Dirk, additional, Heinkelein, Martin, additional, and Rethwilm, Axel, additional

Published
2003
Full Text
View/download PDF

24. Vectors for gene expression and amplification in the yeast <TOGGLE>Yarrowia lipolytica</TOGGLE>

Author

Juretzek, Thomas, Dall, Marie-Thérèse Le, Mauersberger, Stephan, Gaillardin, Claude, Barth, Gerold, and Nicaud, Jean-Marc

Abstract

New vector systems were developed for gene expression in Y. lipolytica. These plasmids contain: (a) as integration target sequences, either a rDNA region or the long terminal repeat zeta of the Y. lipolytica retrotransposon Ylt1; (b) the YlURA3 gene as selection marker for Y. lipolytica, either as the non-defective ura3d1 allele for single integration or the promotor truncated ura3d4 allele for multiple integration; (c) the inducible ICL1 or XPR2 promoters for gene expression; and (d) unique restriction sites for gene insertion. Multiple plasmid integration occurred as inserted tandem-repeats, which are present at 3–39 copies per cell. A correlation between gene copy number and the expressed enzyme activity was demonstrated with Escherichia coli lacZ as reporter gene under the control of the regulated ICL1 promoter. Increases in copy numbers from 5 to 13 for the lacZ expression cassettes resulted in an up to 10–11-fold linear increase of the β-galactosidase activity in multicopy transformants during their growth on ethanol or glucose, compared with the low-copy replicative plasmid transformants (1.6 plasmid copies). These new tools will enhance the interest in Y. lipolytica as an alternative host for heterologous protein production. Copyright © 2000 John Wiley & Sons, Ltd.

Published
2001

Catalog

Books, media, physical & digital resources
See catalog results