6 results on '"Junzhi Duan"'
Search Results
2. Overexpression of OsMYB48-1, a novel MYB-related transcription factor, enhances drought and salinity tolerance in rice.
- Author
-
Haiyan Xiong, Jinjie Li, Pengli Liu, Junzhi Duan, Yan Zhao, Xiao Guo, Yang Li, Hongliang Zhang, Jauhar Ali, and Zichao Li
- Subjects
Medicine ,Science - Abstract
MYB-type transcription factors (TFs) play essential roles in plant growth, development and respond to environmental stresses. Role of MYB-related TFs of rice in drought stress tolerance is not well documented. Here, we report the isolation and characterization of a novel MYB-related TF, OsMYB48-1, of rice. Expression of OsMYB48-1 was strongly induced by polyethylene glycol (PEG), abscisic acid (ABA), H2O2, and dehydration, while being slightly induced by high salinity and cold treatment. The OsMYB48-1 protein was localized in the nucleus with transactivation activity at the C terminus. Overexpression of OsMYB48-1 in rice significantly improved tolerance to simulated drought and salinity stresses caused by mannitol, PEG, and NaCl, respectively, and drought stress was caused by drying the soil. In contrast to wild type plants, the overexpression lines exhibited reduced rate of water loss, lower malondialdehyde (MDA) content and higher proline content under stress conditions. Moreover, overexpression plants were hypersensitive to ABA at both germination and post-germination stages and accumulated more endogenous ABA under drought stress conditions. Further studies demonstrated that overexpression of OsMYB48-1 could regulate the expression of some ABA biosynthesis genes (OsNCED4, OsNCED5), early signaling genes (OsPP2C68, OSRK1) and late responsive genes (RAB21, OsLEA3, RAB16C and RAB16D) under drought stress conditions. Collectively, these results suggested that OsMYB48-1 functions as a novel MYB-related TF which plays a positive role in drought and salinity tolerance by regulating stress-induced ABA synthesis.
- Published
- 2014
- Full Text
- View/download PDF
3. Natural variation of DROT1 confers drought adaptation in upland rice
- Author
-
Xingming Sun, Haiyan Xiong, Conghui Jiang, Dongmei Zhang, Zengling Yang, Yuanping Huang, Wanbin Zhu, Shuaishuai Ma, Junzhi Duan, Xin Wang, Wei Liu, Haifeng Guo, Gangling Li, Jiawei Qi, Chaobo Liang, Zhanying Zhang, Jinjie Li, Hongliang Zhang, Lujia Han, Yihua Zhou, Youliang Peng, and Zichao Li
- Subjects
Plant Breeding ,Multidisciplinary ,General Physics and Astronomy ,Oryza ,General Chemistry ,Cellulose ,General Biochemistry, Genetics and Molecular Biology ,Droughts ,Genome-Wide Association Study - Abstract
Upland rice is a distinct ecotype that grows in aerobic environments and tolerates drought stress. However, the genetic basis of its drought resistance is unclear. Here, using an integrative approach combining a genome-wide association study with analyses of introgression lines and transcriptomic profiles, we identify a gene, DROUGHT1 (DROT1), encoding a COBRA-like protein that confers drought resistance in rice. DROT1 is specifically expressed in vascular bundles and is directly repressed by ERF3 and activated by ERF71, both drought-responsive transcription factors. DROT1 improves drought resistance by adjusting cell wall structure by increasing cellulose content and maintaining cellulose crystallinity. A C-to-T single-nucleotide variation in the promoter increases DROT1 expression and drought resistance in upland rice. The potential elite haplotype of DROT1 in upland rice could originate in wild rice (O. rufipogon) and may be beneficial for breeding upland rice varieties.
- Published
- 2021
4. OsMIOX, a myo-inositol oxygenase gene, improves drought tolerance through scavenging of reactive oxygen species in rice (Oryza sativa L.)
- Author
-
Junzhi Duan, Hongliang Zhang, Jinjie Li, Pengli Liu, Minghui Zhang, Jauhar Ali, Zichao Li, and Haiyan Xiong
- Subjects
Oxygenase ,Time Factors ,Proline ,Drought tolerance ,Molecular Sequence Data ,Plant Science ,Biology ,Upland rice ,Sodium Chloride ,medicine.disease_cause ,Plant Roots ,Inositol oxygenase ,Gene Expression Regulation, Enzymologic ,chemistry.chemical_compound ,Plant Growth Regulators ,Gene Expression Regulation, Plant ,Botany ,Genetics ,medicine ,Amino Acid Sequence ,Abscisic acid ,Peroxidase ,Plant Proteins ,Oryza sativa ,Sequence Homology, Amino Acid ,Reverse Transcriptase Polymerase Chain Reaction ,fungi ,Inositol Oxygenase ,food and beverages ,Water ,Oryza ,General Medicine ,Hydrogen Peroxide ,Catalase ,Oxidants ,Plants, Genetically Modified ,Genetically modified rice ,Adaptation, Physiological ,Droughts ,Cold Temperature ,chemistry ,Biochemistry ,Reactive Oxygen Species ,Agronomy and Crop Science ,Oxidative stress ,Abscisic Acid - Abstract
Myo-inositol oxygenase (MIOX), a unique monooxygenase, catalyzes the oxidation of myo-inositol to d-glucuronic acid. However, the protective role of MIOX in plants against oxidative stress or drought stress remains unknown. In this study, the functional characterization of MIOX obtained from the cDNA library of upland rice (Oryza sativa L. cv. IRAT109), was performed. OsMIOX was expressed predominantly in the roots and induced by drought, H₂O₂, salt, cold and abscisic acid. The transgenic rice lines overexpressing OsMIOX showed obviously improved growth performance in the medium containing 200 mM mannitol. Further, the survival rate of leaves from the transgenic rice lines was significantly higher than that of the wild type plants under polyethylene glycol treatment. It was discovered that the activity of ROS-scavenging enzymes and proline content, as well as the transcript levels of many ROS scavenging genes were significantly increased in transgenic plants compared to the wild type plants under drought stress conditions. Together, these data suggest that OsMIOX has a specific function in drought stress tolerance by decreasing oxidative damage.
- Published
- 2012
5. Overexpression of OsMYB48-1, a Novel MYB-Related Transcription Factor, Enhances Drought and Salinity Tolerance in Rice
- Author
-
Yan Zhao, Jauhar Ali, Xiao Guo, Yang Li, Hongliang Zhang, Pengli Liu, Junzhi Duan, Zichao Li, Haiyan Xiong, and Jinjie Li
- Subjects
Environmental Impacts ,Salinity ,Agricultural Biotechnology ,Gene Expression ,Genetically modified crops ,Soil Chemistry ,Biochemistry ,Transactivation ,chemistry.chemical_compound ,Gene Expression Regulation, Plant ,Molecular Cell Biology ,MYB ,Abscisic acid ,Plant Proteins ,Multidisciplinary ,Ecology ,Genetically Modified Organisms ,food and beverages ,Agriculture ,Plants, Genetically Modified ,Droughts ,Up-Regulation ,Cell biology ,Chemistry ,Physical Sciences ,Medicine ,Research Article ,Transcriptional Activation ,Science ,Active Transport, Cell Nucleus ,Crops ,Biology ,Agricultural Production ,Stress, Physiological ,Plant-Environment Interactions ,Botany ,Genetics ,Environmental Chemistry ,Gene Regulation ,Proline ,Transcription factor ,Cell Nucleus ,Plant Ecology ,Ecology and Environmental Sciences ,fungi ,Wild type ,Biology and Life Sciences ,Proteins ,Oryza ,Cell Biology ,Chemical Properties ,chemistry ,Rice ,Abscisic Acid ,Transcription Factors ,Cereal Crops - Abstract
MYB-type transcription factors (TFs) play essential roles in plant growth, development and respond to environmental stresses. Role of MYB-related TFs of rice in drought stress tolerance is not well documented. Here, we report the isolation and characterization of a novel MYB-related TF, OsMYB48-1, of rice. Expression of OsMYB48-1 was strongly induced by polyethylene glycol (PEG), abscisic acid (ABA), H2O2, and dehydration, while being slightly induced by high salinity and cold treatment. The OsMYB48-1 protein was localized in the nucleus with transactivation activity at the C terminus. Overexpression of OsMYB48-1 in rice significantly improved tolerance to simulated drought and salinity stresses caused by mannitol, PEG, and NaCl, respectively, and drought stress was caused by drying the soil. In contrast to wild type plants, the overexpression lines exhibited reduced rate of water loss, lower malondialdehyde (MDA) content and higher proline content under stress conditions. Moreover, overexpression plants were hypersensitive to ABA at both germination and post-germination stages and accumulated more endogenous ABA under drought stress conditions. Further studies demonstrated that overexpression of OsMYB48-1 could regulate the expression of some ABA biosynthesis genes (OsNCED4, OsNCED5), early signaling genes (OsPP2C68, OSRK1) and late responsive genes (RAB21, OsLEA3, RAB16C and RAB16D) under drought stress conditions. Collectively, these results suggested that OsMYB48-1 functions as a novel MYB-related TF which plays a positive role in drought and salinity tolerance by regulating stress-induced ABA synthesis.
- Published
- 2014
6. Advances in Cold Tolerance Genes and Their Application in Genetic Engineering of Plant for Cold Tolerance.
- Author
-
Junzhi DUAN, Ying LI, Lei ZHOU, Yinghua PAN, Mingzhong ZHAO, and Yinling REN
- Subjects
- *
GENETIC engineering , *CROP yields , *AGRICULTURAL chemicals , *SCIENCE , *TECHNOLOGY - Abstract
Low temperature is one of the main environmental stress factors influencing plant growth and development and crop yield. Cold tolerance genes and progress of their application in genetic engineering of plant for cold tolerance were reviewed comprehensively and systematically from the aspect of genes that are involved in biosynthesis of osmotic substances, genes coding fatty acid desaturation enzymes, antifreeze protein genes, genes coding antioxidant enzymes and so on, aiming at laying the foundation for genetic improvement of cold tolerance and breeding of plants. [ABSTRACT FROM AUTHOR]
- Published
- 2015
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.