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2. Catalytic Nanomedicine. Cu/TiO2–SiO2 Nanoparticles as Treatment of Diabetic Foot Ulcer: A Case Report

Author

Junqueira Virginia Berlanga Campos, Rahimi Nahid, Hashemi Mohammad, Alejandro Velázquez-Muñoz Javier, Liu Hui-Hui, M. Campo Giuseppe, Bahari Gholamreza, You Shou-jiang, Li Tianzhong, Murad Neif, D`Ascola Angela, López-Goerne Tessy, Mehdi Hashemi Seyed, Bacci Marcelo Rodrigues, Mandraffino Giuseppe, Reyes-González Jesús, Jin Xu, Azizi Mahsa, Qu Xinyuan, Chauhan Ritika, Zhang Chunyuan, Li Zheng, Yilin Guo, Saitta Antonino, Zatta Simone Meneghetti, Madhumalini M., Zhang Liping, Sun Hexu, Chauhan Vinita, Shi Jijun, Du Lei, Feder David, Cao Yong-jun, Avenoso Angela, Armando Pérez-Dávalos Luis, Kumar Dhaked Ram, Rocha Roberto Odebrecht, Sonkar Priyanka, Devi T. Meera, Fonseca Fernando Luiz Affonso, Adami Fernando, Alves Beatriz da Costa Aguiar, Yang Mengsu, Campo Salvatore, Ramírez-Olivares Paola, Bruschetta Giuseppe, Dong Weichao, Azzalis Ligia Ajaime, Chagas Antonio Carlos Palandri, and Scuruchi Michele

Subjects
Chemistry, Biomedical Engineering, Medicine (miscellaneous), Nanoparticle, Bioengineering, 02 engineering and technology, 021001 nanoscience & nanotechnology, medicine.disease, 030226 pharmacology & pharmacy, Catalysis, 03 medical and health sciences, 0302 clinical medicine, Diabetic foot ulcer, medicine, Nanomedicine, 0210 nano-technology, Nuclear chemistry
Abstract

Introduction: Diabetes mellitus is one of the most important health issues worldwide. The rising incidence of the disease has resulted in a parallel increase of complications such as diabetic foot ulcers (DFU). It is estimated that 25% of patients with diabetes will develop foot ulcers over a lifetime. In Mexico, this lifetime incidence is about 40% to 50%, and 20% of the cases will require amputations. DFU can result in staggering financial burdens for the healthcare system and the patient. Therapies that promote rapid and complete healing and reduce the need for expensive surgical procedures would impact these costs substantially. Methods: Cu/TiO2–SiO2 nanoparticles were synthesized by T. López et al. sol-gel patented method. After synthesis, they were characterized using transmission electron microscopy (TEM) and X-ray diffraction. Then, they were embedded in a polymeric gel matrix. The Cu/TiO2–SiO2 nanogel was used as conservative therapy for a chronic non-healing DFU on a 62-year old female with several comorbidities and chronic complications of diabetes. Wound debridement was performed prior to nanogel administration. The nanogel was applied over the ulcer on alternate days for an initial period of 2 weeks and then continued for 10 months. Results: Significant improvement was observed in the wound healing process since the first applications. The infection was limited and tissue regeneration was enhanced until complete healing of the ulcer. Conclusion: Cu/TiO2–SiO2 nanogel therapy enhanced reepithelialization and healing of the DFU. The successful outcome allowed to avoid the amputation that was proposed for the patient.

Published
2020
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3. Mortalidade neonatal em Luanda, Angola: o que pode ser feito para sua redução?

Author

Rodrigues, Ema Cunha, primary, Alves, Beatriz Da Costa Aguiar, primary, Veiga, Glaucia Luciano da, primary, Adami, Fernando, primary, Carlesso, Juliana Spat, primary, Figueiredo, Francisco Winter Dos Santos, primary, Azzalis, Ligia Ajaime, primary, Junqueira, Virginia Berlanga Campos, primary, Fonseca, Alexandre Luiz Affonso, primary, and Fonseca, Fernando Luiz Affonso, primary

Published
2019
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4. Application of Remote Ischemic Preconditioning in Patients Undergoing Chemotherapy with Anthracyclines

Author

Zatta, Simone Meneghetti, primary, Murad, Neif, additional, Alves, Beatriz da Costa Aguiar, additional, Azzalis, Ligia Ajaime, additional, Junqueira, Virginia Berlanga Campos, additional, Rocha, Roberto Odebrecht, additional, Bacci, Marcelo Rodrigues, additional, Feder, David, additional, Chagas, Antonio Carlos Palandri, additional, Adami, Fernando, additional, and Fonseca, Fernando Luiz Affonso, additional

Published
2019
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5. Determination of vitamin D in tears of healthy individuals by the electrochemiluminescence method

Author

Lai, Yu Tsun, primary, Cerquinho, Renato Galão, additional, Perez, Matheus Moreira, additional, Alves, Beatriz da Costa Aguiar, additional, Pereira, Edimar Cristiano, additional, Azzalis, Ligia Ajaime, additional, Junqueira, Virginia Berlanga Campos, additional, Soares, Lismeia Raimundo, additional, and Fonseca, Fernando Luiz Affonso, additional

Published
2019
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10. Impact of Selenium Supplementation in Neutropenia and Immunoglobulin Production in Childhood Cancer Patients

Author

Rocha, Katya Cristina, primary, Vieira, Maria Luiza dos Santos, additional, Beltrame, Registila Libania, additional, Cartum, Jairo, additional, Alves, Sarah Isabel P. M. do N., additional, Azzalis, Ligia Ajaime, additional, Junqueira, Virginia Berlanga Campos, additional, Pereira, Edimar Cristiano, additional, and Fonseca, Fernando Luiz Affonso, additional

Published
2016
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11. Adenomioepitelioma com transformações malignas diagnosticado por imuno-histoquímica: relato de caso

Author

Rossetti,Claudia, Azzalis,Ligia Ajaime, Junqueira,Virginia Berlanga Campos, Guidoni,Regina Maria, Gomes,Mara Regina Alcalá, and Fonseca,Fernando Luiz Affonso

Subjects
Malignant, Adenomioepitelioma, Carcinoma, Mama, Adenomyoepithelioma, Breast, Malignidade
Abstract

Adenomyoepitheliomas (AMEs) are rare breast lesions, which are characterized by the proliferation of epithelial and myoepithelial cells. They are circumscribed lesions, which can be clinically and mammographically detected. However, when they are indistinctly limited, differential diagnosis with malignant lesions becomes difficult. In this paper, we report a case of adenomyoepithelioma with malignant transformation of the myoepithelial component, a rare histological type that arises from the malignant transformation of epithelial, myoepithelial, or both elements. Its course and prognosis are uncertain. Mastectomy with sentinel lymph node biopsy followed by radiotherapy are the most effective treatment approaches. Adenomioepiteliomas (AMEs) são lesões raras na mama, caracterizadas pela proliferação de elementos epiteliais e mioepiteliais. Clínica e mamograficamente apresentam-se como lesões bem delimitadas, que, quando assumem aspecto mal delimitado, tornam difícil o diagnóstico diferencial com lesões malignas. Relatamos um caso de AME com transformação maligna do componente mioepitelial, tipo histológico ainda mais raro, que surge da transformação maligna de elementos epiteliais, mioepiteliais ou ambos. Seu curso e seu prognóstico são incertos, contudo, mastectomia com pesquisa de linfonodo sentinela seguidos de radioterapia constituem a forma mais efetiva de tratamento.

Published
2011

12. Short courses of mechanical ventilation with high-O 2 levels in elderly rat lungs

Author

Cavassani, Sâmia Santos, Junqueira, Virginia Berlanga Campos, Moraes, Juliana Britto, Luzo, Karen Kristina, Silva, Claudeny Matos Assis, Barros, Maciel, Marinho, Márcia [UNESP], Simões, Ricardo Santos, Oliveira-Júnior, Itamar Souza, Universidade Federal de São Paulo (UNIFESP), and Universidade Estadual Paulista (UNESP)

Subjects
Ventilator-induced lung injury, Aging, Oxidative stress, Lung injury, Neutrophil infiltration, Rats
Abstract

Made available in DSpace on 2022-04-28T22:37:00Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-04-01 Purpose: To evaluate the effects of mechanical ventilation (MV) of high-oxygen concentration in pulmonary dysfunction in adult and elderly rats. Methods: Twenty-eight adult (A) and elderly (E), male rats were ventilated for 1 hour (G-AV1 and G-EV1) or for 3 hours (G-AV3 and G-EV3). A and E groups received a tidal volume of 7 mL/kg, a positive end-expiratory pressure of 5 cm H2O, respiratory rate of 70 cycles per minute, and an inspiratory fraction of oxygen of 1. We evaluated total protein content and malondialdehyde in bronchoalveolar lavages (BAL) and performed lung histomorphometrical analyses. Results: In G-EV1 animals, total protein in BAL was higher (33.0±1.9 μg/mL) compared with G-AV1 (23.0±2.0 μg/mL). Upon 180 minutes of MV, malondialdehyde levels increased in elderly (G-EV3) compared with adult (G-AV3) groups. Malondialdehyde and total proteins in BAL after 3 hours of MV were higher in elderly group than in adults. In G-EV3 group we observed alveolar septa dilatation and signifcative increase in neutrofles number in relation to adult group at 60 and 180 minutes on MV. Conclusion: A higher fraction of inspired oxygen in short courses of mechanical ventilation ameliorates the parameters studied in elderly lungs. Department of Preventive Medicine UNIFESP, Sao Paulo-SP Department of Biological Science UNIFESP, Diadema-SP Division of Anesthesiology, Pain and Intensive Therapy UNIFESP, Sao Paulo-SP Department of Production Support and Animal Health Paulista State University, Araçatuba-SP Department of Morphology and Genetics Histology Division UNIFESP, Sao Paulo-SP Division of Geriatrics and Gerontology UNIFESP, Sao Paulo-SP Department of Production Support and Animal Health Paulista State University, Araçatuba-SP

Published
2011

13. Avaliação do hiperinsulinismo em amostra de crianças pré-puberes

Author

Mieldazis,Stephanye Felicye Armecy, Azzalis,Ligia Ajaime, Junqueira,Virginia Berlanga Campos, Souza,Fabíola Isabel Suano, Sarni,Roseli Oselka Sacardo, and Fonseca,Fernando Luiz Affonso

Subjects
Coleta de amostras sanguíneas, glicose, hiperinsulinismo
Abstract

OBJETIVOS: Determinar a relação entre o índice de massa corporal (IMC), o homeostasis model assessment - insulin resistance (HOMA-IR) e a insulinemia. MÉTODOS: Realizou-se um estudo observacional prospectivo transversal com 132 crianças pré-púberes em idade escolar e residentes no município de Santo André (SP). Fez-se a avaliação antropométrica e a mensuração da glicemia, da insulinemia e do índice HOMA-IR. RESULTADOS: Dentre as 132 crianças avaliadas, 78 eram meninas (59,1%) e 54 eram meninos (40,9%), com média de idade de 8,7 anos e média de IMC de 13,7 kg/m². Observou-se uma associação significativa e positiva entre HOMA-IR e IMC, insulina e IMC, peso e HOMA e entre insulina e peso; também foi constatado que, quanto maior for o IMC, maior será o valor de HOMA. CONCLUSÕES: Os resultados do presente estudo permitem concluir que há uma forte associação entre o hiperinsulinismo e a obesidade, devendo ser tomadas algumas medidas para evitar o ganho de peso durante a infância e a adolescência.

Published
2010

14. Breast cancer with choriocarcinomatous features

Author

Rossetti,Claudia, Sacchi,Renata Silvia, Junqueira,Virginia Berlanga Campos, and Fonseca,Fernando Luiz Affonso

Subjects
Breast, Carcinomas, Choriocarcinomatous features
Abstract

This report describes the case of a 45 year-old patient with breast carcinoma characterized by choriocarcinomatous features, presence of bizarre anaplastic cells with areas of necrosis and multinucleated giant cells. The immuno-histochemical analysis showed positivity for chromogranin and β-HCG. The tumor size was 5.7 cm at the largest axis and it was refractory to initial neo-adjuvant treatment. Mastectomy was performed and after microscopic and immuno-histochemical examination it was diagnosed as carcinoma with choriocarcinomatous features. These lesions are rare and liable to synthesize hormonal substances not specific to breast tissue. Its diagnosis is highly significant to the therapeutic and prognostic approach insofar as these lesions are potentially aggressive.

Published
2009

15. Supplementation with Selenium Can Influence Nausea, Fatigue, Physical, Renal, and Liver Function of Children and Adolescents with Cancer

Author

Vieira, Maria Luiza dos Santos, primary, Fonseca, Fernando Luiz Affonso, additional, Costa, Larissa Grossi, additional, Beltrame, Registila Libania, additional, Chaves, Carolina Machado de Sousa, additional, Cartum, Jairo, additional, Alves, Sarah Isabel P. M. do N., additional, Azzalis, Ligia Ajaime, additional, Junqueira, Virginia Berlanga Campos, additional, Pereria, Edimar Cristiano, additional, and Rocha, Katya Cristina, additional

Published
2015
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16. OUTCOMES OF ACCIDENTS AT WORK WITH EXPOSURE

Author

Machi Junior, Amaury, primary, Quiaios, André, additional, Domingues, José Nuno, additional, Ferreira, Ana, additional, Paixão, Susana, additional, Sá, Nelson Leite, additional, Azzalis, Ligia Ajaime, additional, Junqueira, Virginia Berlanga Campos, additional, Silva, Odair Ramos da, additional, and Fonseca, Fernando Luiz Affonso, additional

Published
2014
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18. Segurança contra incêndio em unidades básicas de saúde

Author

Machi Junior, Amaury, primary, Quiaios, André, additional, Domingues, José Nuno, additional, Ferreira, Ana, additional, Paixão, Susana, additional, Sá, Nelson Leite, additional, Azzalis, Ligia Ajaime, additional, Junqueira, Virginia Berlanga Campos, additional, Silva, Odair Ramos da, additional, Vicente, Maria Teresa Conceição, additional, and Fonseca, Fernando Luiz Affonso, additional

Published
2014
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19. Hematological and hepatic effects of vascular epidermal growth factor (VEGF) used to stimulate hair growth in an animal model

Author

Gnann, Laís Angelo, primary, Castro, Rafael Ferreira, additional, Azzalis, Ligia Ajaime, additional, Feder, David, additional, Perazzo, Fabio Ferreira, additional, Pereira, Edimar Cristiano, additional, Rosa, Paulo César Pires, additional, Junqueira, Virginia Berlanga Campos, additional, Rocha, Katya Cristina, additional, Machado, Carlos D’ Aparecida, additional, Paschoal, Francisco Camargo, additional, de Abreu, Luiz Carlos, additional, Valenti, Vitor Engrácia, additional, and Fonseca, Fernando Luiz Affonso, additional

Published
2013
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21. Occupational Infection by the Hepatitis B Virus: Risk and Prevention Measures

Author

Lima, Bruno Francisco Real de, primary, Waffae, Maria Cristina, additional, Figueiredo, Elizabeth Niglio de, additional, Filipinni, Rosangela, additional, Luz, Maria Claudia De Brito, additional, Azzalis, Ligia Ajaime, additional, Junqueira, Virginia Berlanga Campos, additional, Fonseca, Fernando Luiz Affonso, additional, and Chaves, Loide Corina, additional

Published
2013
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22. Comparative study between immunoturbidimetric and latex agglutination methods for the detection of rheumatoid factor

Author

Rocha, Katya Cristina, primary, Brinque, Luiz Alberto da Fonseca, additional, Oliveira, Claudia G. B., additional, Sant'Anna, Aleksandra Vanessa Lambiasi, additional, Fonseca, Alexandre Luiz Affonso, additional, Azzalis, Ligia Ajaime, additional, Pereira, Edimar Cristiano, additional, Junqueira, Virginia Berlanga Campos, additional, Beltrame, Registila Libania, additional, and Fonseca, Fernando Luiz Affonso, additional

Published
2013
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23. TETRAPARETIC CHILDREN AND CAREGIVERS: FEATURING THE PROFILE AND ACCESSIBILITY ASSISTIVE TECHNOLOGY

Author

Alcassa, Tatiana Cristina, primary, Filipini, Rosangela, additional, Garcia, Jesus Carlos Delgado, additional, Amarante, Sandra Terezinha, additional, Adami, Fernando, additional, Luz, Maria Claudia Brito, additional, Pimentel, Renata Pimentel Macedo, additional, Azzalis, Ligia Ajaime, additional, Junqueira, Virginia Berlanga Campos, additional, and Fonseca, Fernando Luiz Affonso, additional

Published
2013
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26. Triagem sorológica e influência do conhecimento sobre a dengue em pacientes do ambulatório de especialidades do SUS

Author

Vilas Boas, Viviane Aparecida, primary, Rocha, Katya Cristina, additional, Oliveira, Claudia Giorgia Bronzatti de, additional, Sant'Anna, Aleksandra Vanessa Lambiasi, additional, Azzalis, Ligia Ajaime, additional, Beltrame, Registila Libânia, additional, Junqueira, Virginia Berlanga Campos, additional, and Fonseca, Fernando Luiz Affonso, additional

Published
2011
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31. Aspectos histomorfológicos e respiratórios em modelo de lesão pulmonar aguda por sepse em ratos tratados com pentoxifilina

Author

Oliveira, Wagner Rogerio Souza de, primary, Cavassani, Sâmia Santos, additional, Maganhin, Carla Cristina, additional, Carbonel, Adriana Aparecida Ferraz, additional, Simões, Manuel Jesus, additional, Simões, Ricardo Santos, additional, Junqueira, Virginia Berlanga Campos, additional, and Oliveira-Júnior, Itamar Souza de, additional

Published
2009
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32. Potentially hazardous waste produced at home.

Author

Chaves LC, de Campos LM, Filipini R, de Abreu LC, Valenti VE, Azzalis LA, Junqueira VB, Sena DF, Goulart FC, and Fonseca FL

Abstract

Background: The purpose of this study was to identify the sources of waste generation household consisting of biological material and to investigate the knowledge presented by those responsible for the generation of waste in the home environment on the potential health risk human and environmental., Method: It is a quantitative survey performed in Parque Capuava, Santo André (SP). The questionnaire was administered by the community employers and nursing students during the consultation with nursing supervision through interview question/answer. The exclusion criteria were patients who were not in the area served by the Basic Health Unit which covers the area of Pq Capuava. The sample was consisted of 99 persons and the data collection a questionnaire was used., Results: We observed that 63.3% of people said to use disposables, with the majority (58.7%) of these use the public collection as the final destination of these materials. It was reported that 73.7% of those surveyed reported having knowledge about the risk of disease transmission. Public awareness of the importance of proper packaging and disposal of potentially hazardous household waste may contribute significantly to the preservation of human and environmental health and this procedure can be performed and supervised by professional nurses., Conclusion: We suggest implementation of workshops for community health workers and the general population in order to enhance their knowledge about the storage and disposal of potentially infectious waste generated at home, thereby reducing the potential risk of disease transmission by improper management.

Published
2013
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33. [Histomorphologic and respiratory aspects of acute lung injury in rats induced by experimental sepsis and under pentoxifylline treatment].

Author

Oliveira WR, Cavassani SS, Maganhin CC, Carbonel AA, Simões MJ, Simões RS, Junqueira VB, and Oliveira-Júnior IS

Subjects
Animals, Bronchoalveolar Lavage Fluid, Escherichia coli Infections complications, Escherichia coli Infections drug therapy, Escherichia coli Infections microbiology, Male, Pulmonary Alveoli metabolism, Pulmonary Alveoli pathology, Rats, Rats, Wistar, Respiration, Artificial, Sepsis complications, Sepsis pathology, Acute Lung Injury metabolism, Acute Lung Injury pathology, Acute Lung Injury therapy, Oxygen metabolism, Pentoxifylline therapeutic use, Sepsis drug therapy, Vasodilator Agents therapeutic use
Abstract

Objective: Respiratory repercussion on acute lung injury in a model of induced sepsis intraperitoneally., Methods: Fifteen animals taken at random were submitted to adult male Wistar rats. The rats were randomly divided into 3 groups (n=15): Group C - control group received only mechanical ventilation; Group S - rats received live Escherichia coli (E. coli) intraperitoneally (septic) and after 6 hours they were treated with normal saline infusion and ventilated with a low tidal volume. Group S+PTX - rats received live Escherichia coli intraperitoneally (septic) and after 6 hours they were treated with pentoxifylline (PTX) infusion and ventilated with a low tidal volume. All animals were ventilated during 180 minutes. We analyzed the arterial blood gases, gravimetric indices and histomorphometric analysis., Results: Blood gases, wet to dry ratios, and total protein concentrations in the bronchoalveolar lavage were analyzed in all experimental groups. In the end of the experiment the partial pressure of oxygen was higher in the GS+PTX (460,0 +/- 38,2 mmHg) compared with GS (336,0 +/- 14,6 mmHg). Pentoxifylline with low tidal volume attenuated significantly total protein in the bronchoalveolar lavage. The septal diameter was significantly reduced in the group GS compared with group GS+PTX (P < 0,05)., Conclusions: The pentoxifylline ameliorated the oxygenation and decreased the deleterious effects of sepsis in the associated mechanical ventilation.

Published
2009
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