212 results on '"Junqi Huang"'
Search Results
2. Prevalence and molecular characteristics of ceftazidime-avibactam resistance among carbapenem-resistant Pseudomonas aeruginosa clinical isolates
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Yili Chen, Guoxiu Xiang, Pingjuan Liu, Xianling Zhou, Penghao Guo, Zhongwen Wu, Juhua Yang, Peisong Chen, Junqi Huang, and Kang Liao
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Carbapenem-resistant Pseudomonas aeruginosa ,Ceftazidime-avibactam resistance ,Whole-genome sequencing ,Microbiology ,QR1-502 - Abstract
ABSTRACT: Objectives: Resistance against ceftazidime-avibactam (CZA) in carbapenem-resistant Pseudomonas aeruginosa (CRPA) is emerging. This study was aimed at detecting the prevalence and molecular characteristics of CZA-resistant CRPA clinical isolates in Guangdong Province, China. Methods: The antimicrobial susceptibility profile of these strains was determined. A subset of 16 CZA-resistant CRPA isolates was analysed by whole-genome sequencing (WGS). Genetic surroundings of carbapenem resistance genes and pan-genome-wide association analysis were further studied. Results: Of the 250 CRPA isolates, CZA resistance rate was 6.4% (16/250). The minimum inhibitory concentration (MIC) of CZA range was from 0.25 to >256 mg/L. MIC50 and MIC90 were 2/4 and 8/4 mg/L, respectively. Among the 16 CZA-resistant CRPA strains, 31.3% (5/16) of them carried class B carbapenem resistance genes, including blaIMP-4, blaIMP-45, and blaVIM-2, located on IncP-2 megaplasmids or chromosomes, respectively. Pan-genome-wide association analysis of accessory genes for CZA-susceptible or -resistant CRPA isolates showed that PA1874, a hypothetical protein containing BapA prefix-like domain, was enriched in CZA-resistant group significantly. Conclusions: Class B carbapenem resistance genes play important roles in CZA resistance. Meanwhile, the PA1874 gene may be a novel mechanism involving in CZA resistance. It is necessary to continually monitor CZA-resistant CRPA isolates.
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- 2024
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3. A Visual Distance-Based Capillary Immunoassay Using Biomimetic Polymer Nanoparticles for Highly Sensitive and Specific C-Reactive Protein Quantification
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Ruodong Huang, Zhenbo Liu, Xinlin Jiang, Junqi Huang, Ping Zhou, Zongxia Mou, Dong Ma, and Xin Cui
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distance-based immunoassay ,C-reactive protein ,biomimetic nanoparticles ,specificity ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
The low-cost daily monitoring of C-reactive protein (CRP) levels is crucial for screening acute inflammation or infections as well as managing chronic inflammatory diseases. In this study, we synthesized novel 2-Methacryloyloxy ethyl phosphorylcholine (MPC)-based biomimetic nanoparticles with a large surface area to develop a visual CRP-quantification assay using affordable glass capillaries. The PMPC nanoparticles, synthesized via reflux precipitation polymerization, demonstrated multivalent binding capabilities, enabling rapid and specific CRP capture. In the presence of CRP, PMPC nanoparticles formed sandwich structures with magnetic nanoparticles functionalized with CRP antibodies, thereby enhancing detection sensitivity and specificity. These sandwich complexes were magnetically accumulated into visible and quantifiable stacks within the glass capillaries, allowing for the rapid, sensitive, and specific quantification of CRP concentrations with a detection limit of 57.5 pg/mL and a range spanning from 0 to 5000 ng/mL. The proposed visual distance-based capillary biosensor shows great potential in routine clinical diagnosis as well as point-of-care testing (POCT) in resource-limited settings.
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- 2024
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4. Fractal-Based Multi-Criteria Feature Selection to Enhance Predictive Capability of AI-Driven Mineral Prospectivity Mapping
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Tao Sun, Mei Feng, Wenbin Pu, Yue Liu, Fei Chen, Hongwei Zhang, Junqi Huang, Luting Mao, and Zhiqiang Wang
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fractal ,mineral prospectivity mapping ,feature selection ,AI-driven ,machine learning ,Gannan district ,Thermodynamics ,QC310.15-319 ,Mathematics ,QA1-939 ,Analysis ,QA299.6-433 - Abstract
AI-driven mineral prospectivity mapping (MPM) is a valid and increasingly accepted tool for delineating the targets of mineral exploration, but it suffers from noisy and unrepresentative input features. In this study, a set of fractal and multifractal methods, including box-counting calculation, concentration–area fractal modeling, and multifractal analyses, were employed to excavate the underlying nonlinear mineralization-related information from geological features. Based on these methods, multiple feature selection criteria, namely prediction–area plot, K-means clustering, information gain, chi-square, and the Pearson correlation coefficient, were jointly applied to rank the relative importance of ore-related features and their fractal representations, so as to choose the optimal input feature dataset readily used for training predictive AI models. The results indicate that fault density, the multifractal spectrum width (∆α) of the Yanshanian intrusions, information dimension (D1) of magnetic anomalies, correlation dimension (D2) of iron-oxide alteration, and the D2 of argillic alteration serve as the most effective predictor features representative of the corresponding ore-controlling elements. The comparative results of the model assessment suggest that all the AI models trained by the fractal datasets outperform their counterparts trained by raw datasets, demonstrating a significant improvement in the predictive capability of fractal-trained AI models in terms of both classification accuracy and predictive efficiency. A Shapley additive explanation was employed to trace the contributions of these features and to explain the modeling results, which imply that fractal representations provide more discriminative and definitive feature values that enhance the cognitive capability of AI models trained by these data, thereby improving their predictive performance, especially for those indirect predictor features that show subtle correlations with mineralization in the raw dataset. In addition, fractal-trained models can benefit practical mineral exploration by outputting low-risk exploration targets that achieve higher capturing efficiency and by providing new mineralization clues extracted from remote sensing data. This study demonstrates that the fractal representations of geological features filtered by multi-criteria feature selection can provide a feasible and promising means of improving the predictive capability of AI-driven MPM.
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- 2024
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5. Dynamic evolution of ceftazidime–avibactam resistance due to interchanges between blaKPC-2 and blaKPC-145 during treatment of Klebsiella pneumoniae infection
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Yili Chen, Runshi Yang, Penghao Guo, Pingjuan Liu, Jiankai Deng, Zhongwen Wu, Qingping Wu, Junqi Huang, and Kang Liao
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carbapenem resistance ,Klebsiella pneumoniae ,ceftazidime-avibactam resistance ,KPC-2 ,KPC-145 ,Microbiology ,QR1-502 - Abstract
BackgroundThe emergence of ceftazidime–avibactam (CZA) resistance among carbapenem-resistant Klebsiella pneumoniae (CRKP) is of major concern due to limited therapeutic options.MethodsIn this study, 10 CRKP strains were isolated from different samples of a patient with CRKP infection receiving CZA treatment. Whole-genome sequencing (WGS) and conjugation experiments were performed to determine the transferability of the carbapenem resistance gene.ResultsThis infection began with a KPC-2-producing K. pneumoniae (CZA MIC = 2 μg/mL, imipenem MIC ≥ 16 μg/mL). After 20 days of CZA treatment, the strains switched to the amino acid substitution of T263A caused by a novel KPC-producing gene, blaKPC-145, which restored carbapenem susceptibility but showed CZA resistance (CZA MIC ≥ 256 μg/mL, imipenem MIC = 1 μg/mL). The blaKPC-145 gene was located on a 148,185-bp untransformable IncFII-type plasmid. The subsequent use of carbapenem against KPC-145-producing K. pneumoniae infection led to a reversion of KPC-2 production (CZA MIC = 2 μg/mL, imipenem MIC ≥ 16 μg/mL). WGS analysis showed that all isolates belonged to ST11-KL47, and the number of SNPs was 14. This implied that these blaKPC-positive K. pneumoniae isolates might originate from a single clone and have been colonized for a long time during the 120-day treatment period.ConclusionThis is the first report of CZA resistance caused by blaKPC-145, which emerged during the treatment with CZA against blaKPC-2-positive K. pneumoniae-associated infection in China. These findings indicated that routine testing for antibiotic susceptibility and carbapenemase genotype is essential during CZA treatment.
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- 2023
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6. Neoadjuvant Chemotherapy and Expandable Prosthesis Reconstruction to Treat Osteosarcoma around the Knee in Children
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Junqi Huang, Jiajia Cheng, Wenzhi Bi, Meng Xu, Jinpeng Jia, Gang Han, and Wei Wang
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Child ,Expandable prosthesis ,Knee ,Neoadjuvant chemotherapy ,Osteosarcoma ,Orthopedic surgery ,RD701-811 - Abstract
Objective Survival and reconstruction in osteosarcoma is quite challenging. The study aimed to investigate the prognosis in patients treated with neoadjuvant chemotherapy and determine the clinical outcomes of expandable endoprosthesis reconstruction in children. Methods From January 2009 to December 2014, we retrospectively analyzed 29 skeletally immature children (mean age, 10.5 years; range, 6–15 years) with osteosarcoma around the knee. Of the 29 patients who underwent neoadjuvant chemotherapy and limb salvage surgery, an expandable prosthesis was implanted for reconstruction. No patients were missed during follow‐up. The evaluation index involved follow‐up time, complication, functional results, and lengthening procedures. The survivorship and recurrence were assessed by GraphPad Software, and the function was evaluated by the Musculoskeletal Tumor Society (MSTS) scoring system. Results A mean follow‐up time was 8.9 years (range, 6–12 years), and the overall 5‐year survival was 89.1% based on Kaplan–Meier analysis. Three patients suffered a relapse and one underwent amputation. Lung metastasis developed in one patient. At 6 months after the operation, patients had a mean MSTS score of 27 points (range, 24–29). Two patients underwent revision surgery, one for implant infection and one for aseptic loosening. Prognosis is correlated with alkaline phosphatase change after treatment. Conclusions Chemotherapy scheme and limb salvage can achieve high survival rates. This expandable prosthesis was associated with good function and low complication rates. The character of expandability could be a method to overcome discrepancies in the growth period.
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- 2023
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7. An immune risk score predicts progression-free survival of melanoma patients in South China receiving anti-PD-1 inhibitor therapy—a retrospective cohort study examining 66 circulating immune cell subsets
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Peidong Chi, Hang Jiang, Dandan Li, Jingjing Li, Xizhi Wen, Qiyue Ding, Linbin Chen, Xiaoshi Zhang, Junqi Huang, and Ya Ding
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melanoma ,PD-1 ,immune checkpoint blockade inhibitor therapy ,innate immune ,adaptive immune ,Immunologic diseases. Allergy ,RC581-607 - Abstract
IntroductionImmune checkpoint blockade inhibitor (ICI) therapy offers significant survival benefits for malignant melanoma. However, some patients were observed to be in disease progression after the first few treatment cycles. As such, it is urgent to find convenient and accessible indicators that assess whether patients can benefit from ICI therapy.MethodsIn the training cohort, flow cytometry was used to determine the absolute values of 66 immune cell subsets in the peripheral blood of melanoma patients (n=29) before treatment with anti-PD-1 inhibitors. The least absolute shrinkage and selection operator (LASSO) Cox regression model was followed for the efficacy of each subset in predicting progression-free survival. Then we validated the performance of the selected model in validation cohorts (n=20), and developed a nomogram for clinical use.ResultsA prognostic immune risk score composed of CD1c+ dendritic cells and three subsets of T cells (CD8+CD28+, CD3+TCRab+HLA-DR+, CD3+TCRgd+HLA-DR+) with a higher prognostic power than individual features (AUC = 0.825). Using this model, patients in the training cohort were divided into high- and low-risk groups with significant differences in mean progression-free survival (3.6 vs. 12.3 months), including disease control rate (41.2% vs. 91.7%), and objective response rate (17.6% vs. 41.6%). Integrating four-immune cell-subset based classifiers and three clinicopathologic risk factors can help to predict which patients might benefit from anti-PD-1 antibody inhibitors and remind potential non-responders to pursue effective treatment options in a timely way.ConclusionsThe prognostic immune risk score including the innate immune and adaptive immune cell populations could provide an accurate prediction efficacy in malignant melanoma patients with ICI therapy.
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- 2022
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8. An engineered tenogenic patch for the treatment of rotator cuff tear
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Shaoshen Zhu, Jianfeng Hou, Chang Liu, Peng Liu, Ting Guo, Zhengjie Lin, Xianwei Wang, Chunmiao Wu, Dichun Huang, Junqi Huang, Zuyong Wang, and Ronghan He
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Rotator cuff tear ,Tendon ,Tendon-to-bone healing ,Scaffold ,Materials of engineering and construction. Mechanics of materials ,TA401-492 - Abstract
Rotator cuff tear of shoulder is a common tendon injury. Current therapeutic strategies are unsatisfactory because of poor biomechanical properties of the repaired rotator cuff tendons. To solve this problem, a newly biofabricated tenogenic patch by uniaxial cold-drawing technology was applied for the treatment of acute rotator cuff tear and its therapeutic effects were evaluated in vitro and in vivo. The cytocompatibility results indicated that the engineered patch was capable of promoting the spreading, migration and proliferation of tendon fibroblasts in vitro. Stress–strain curves of the sutured patch indicated improved elasticity followed by the yield phenomena, nonlinear plasticity and obviously delaminated rupture. The tenogenic patch also showed significantly enhanced collagen I deposition and better tendon-to-bone healing in the rat rotator cuff tear models in vivo. Furthermore, implantation with the tenogenic patch also promoted the fibrocartilage formation in Safranin-O/Fast green-staining. The analysis of biomechanical properties showed that the tenogenic patch is capable of improving the ultimate load of the repaired tendon. Therefore, the tenogenic patch may constitute an efficient strategy to enhance tendon-to-bone healing for clinical repair applications of rotator cuff tear.
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- 2022
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9. Tumor-suppressive function of EZH2 is through inhibiting glutaminase
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Yongfeng Liu, Cheng-e Tu, Xuxue Guo, Changjie Wu, Chuncai Gu, Qiuhua Lai, Yuxin Fang, Junqi Huang, Zhizhang Wang, Aimin Li, and Side Liu
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Cytology ,QH573-671 - Abstract
Abstract Tumors can use metabolic reprogramming to survive nutrient stress. Epigenetic regulators play a critical role in metabolic adaptation. Here we screened a sgRNA library to identify epigenetic regulators responsible for the vulnerability of colorectal cancer (CRC) cells to glucose deprivation and found that more EZH2-knockout cells survived glucose deprivation. Then, we showed that EZH2 expression was significantly downregulated in response to glucose deprivation in a glucose-sensitive CRC cell line, and EZH2-knockdown cells were more resistant to glucose deprivation. Mechanistically, EZH2 deficiency upregulated the expression of glutaminase (GLS) and promoted the production of glutamate, which in turn led to increased synthesis of intracellular glutathione (GSH) and eventually attenuated the reactive oxygen species (ROS)-mediated cell death induced by glucose deprivation. Although EZH2 functioned as an oncogene in cancer progression and EZH2 knockout abolished colorectal cancer development in a mouse model, here we revealed a mechanistic link between EZH2 and metabolic reprogramming via the direct regulation of GLS expression and observed a negative correlation between EZH2 and GLS expression in colorectal cancer tissues. These findings further confirmed the importance of heterogeneity, provided an explanation for the clinical tolerance of cancer cells to EZH2 inhibitors from the perspective of metabolism, and proposed the possibility of combining EZH2 inhibitors and glutamine metabolism inhibitors for the treatment of cancer.
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- 2021
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10. Prospectivity Mapping of Tungsten Mineralization in Southern Jiangxi Province Using Few-Shot Learning
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Kai Zhou, Tao Sun, Yue Liu, Mei Feng, Jialiang Tang, Luting Mao, Wenbin Pu, and Junqi Huang
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machine learning ,mineral prospectivity mapping ,few-shot learning ,SMOTE ,tungsten mineralization ,southern Jiangxi Province ,Mineralogy ,QE351-399.2 - Abstract
The development of mineral prospectivity mapping (MPM), which aims to outline and prioritize mineral exploration targets, has been spurred by advances in data-driven machine learning algorithms. Supervised data-driven MPM is a typical few-shot task, suffering from a scarcity of labeled data, the over-fitting of models and an uncertainty of predictions. The main objective of this contribution is to propose a robust framework of few-shot learning (FSL), combining data augmentation and transfer learning to enable the generation of prospectivity models with excellent predictive efficiency and low uncertainty. The mineral systems approach was used to transfer a conceptual mineral system into mappable exploration criteria. Synthetic minority over-sampling technique (SMOTE) was employed to augment and balance the labeled dataset, allowing for model pre-training with the large synthetic training dataset of a source domain. The knowledge derived from pre-trained models was then transferred to the target domain by fine-tuning, and the prospectivity model was generated in light of over-fitting and uncertainty assessments. The proposed FSL framework was applied to tungsten prospectivity mapping in southern Jiangxi Province. The results indicated that the SMOTE-ed balanced dataset boosted the classification accuracy in the training process. The FSL models yielded an arch-shaped prediction point pattern which was favorable for focusing potential targets with high probability and low uncertainty. The FSL models achieved a high predictive performance (test AUC = 0.9172) and the lowest quantitative over-fitting value compared to the models derived from the benchmark algorithms of random forest and support vector machine. Four levels of potential targeting zones, considering both predictive efficiency and uncertainty, were extracted from the resulting FSL prospectivity map. The final high-potential and low-risk exploration targets only cover 4.27% of the area, but capture 41.53% of known tungsten deposits, thus achieving a superior predictive performance. This study highlights the capability of FSL framework to control over-fitting and generate high-confidence exploration targets with low levels of uncertainty.
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- 2023
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11. Understanding Anthracycline Cardiotoxicity From Mitochondrial Aspect
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Junqi Huang, Rundong Wu, Linyi Chen, Ziqiang Yang, Daoguang Yan, and Mingchuan Li
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anthracycline ,cardiotoxicity ,mitochondria ,ROS ,ferroptosis ,mitophagy ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Anthracyclines, such as doxorubicin, represent one group of chemotherapy drugs with the most cardiotoxicity. Despite that anthracyclines are capable of treating assorted solid tumors and hematological malignancies, the side effect of inducing cardiac dysfunction has hampered their clinical use. Currently, the mechanism underlying anthracycline cardiotoxicity remains obscure. Increasing evidence points to mitochondria, the energy factory of cardiomyocytes, as a major target of anthracyclines. In this review, we will summarize recent findings about mitochondrial mechanism during anthracycline cardiotoxicity. In particular, we will focus on the following aspects: 1) the traditional view about anthracycline-induced reactive oxygen species (ROS), which is produced by mitochondria, but in turn causes mitochondrial injury. 2) Mitochondrial iron-overload and ferroptosis during anthracycline cardiotoxicity. 3) Autophagy, mitophagy and mitochondrial dynamics during anthracycline cardiotoxicity. 4) Anthracycline-induced disruption of cardiac metabolism.
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- 2022
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12. MicroRNA-20a Suppresses Tumor Proliferation and Metastasis in Hepatocellular Carcinoma by Directly Targeting EZH1
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Qianqian Zhang, Xiaohong Deng, Xiuxin Tang, Ying You, Meihua Mei, Danping Liu, Lian Gui, Yan Cai, Xiaoping Xin, Xiaoshun He, and Junqi Huang
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hepatocellular carcinoma ,microRNA-20a ,EZH1 ,metastasis ,epigenetics ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
PurposeHepatocellular carcinoma (HCC), a worldwide leading cause of morbidity and mortality, is the most frequent primary liver tumor. Most HCC patients are diagnosed with advanced liver cancer, resulting in a very low 5-year survival rate. Thus, there is an urgent need for the development of targeted therapies. In this study, we aimed to investigate the effect and mechanism of the miR-20a/EZH1 axis on the proliferation and metastasis of HCC and the inhibitory effect of the EZH1/EZH2 inhibitor UNC1999 on HCC.Materials and MethodsThe expression of miR-20a in human HCC tissues and cell lines was detected using quantitative real-time PCR (qRT-PCR). The expressions of proteins were analyzed with immunohistochemistry and Western blotting. Luciferase assay was used to verify whether miR-20a targets EZH1 or EZH2. The effect of miR-20a on HCC progression was studied in vivo and in vitro. The tumor inhibitory effect of UNC1999 was confirmed in vivo. CCK8 assay, wound healing assay, cell migration and invasion assay were used to evaluate the synergistic effect of UNC1999 with sorafenib. RNA sequencing (RNA-seq) was performed to screen the differentially expressed genes in the Huh7 and SMMC7721 cell lines after UNC1999, sorafenib, and combination treatments.ResultsIn this study, miR-20a showed a lower expression in both HCC tissues and cell lines. MiR-20a inhibited the proliferation and migration of SMMC7721 and Huh7 cells. The results of the luciferase assay and Western blot analysis revealed that miR-20a directly targeted EZH1, a histone methyltransferase. We demonstrated that miR-20a negatively regulated the expression of EZH1 and inhibited the proliferation and metastasis of HCC by reducing H3K27 methylation. We found UNC1999 inhibited tumor cells proliferation and enhanced the inhibitory effect of sorafenib.ConclusionWe demonstrated that miR-20a suppresses the tumor proliferation and metastasis in HCC by directly targeting EZH1. UNC1999 can inhibit tumor proliferation in vivo and increase the sensitivity of hepatoma cell lines to sorafenib.
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- 2021
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13. Involvement of the Actin Machinery in Programmed Cell Death
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Weida Ren, Wanyu Zhao, Lingbo Cao, and Junqi Huang
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programmed cell death ,actin ,actin-binding proteins ,actin-modulating proteins ,actin machinery ,cytoskeleton ,Biology (General) ,QH301-705.5 - Abstract
Programmed cell death (PCD) depicts a genetically encoded and an orderly mode of cellular mortality. When triggered by internal or external stimuli, cells initiate PCDs through evolutionary conserved regulatory mechanisms. Actin, as a multifunctional cytoskeleton protein that forms microfilament, its integrity and dynamics are essential for a variety of cellular processes (e.g., morphogenesis, membrane blebbing and intracellular transport). Decades of work have broadened our knowledge about different types of PCDs and their distinguished signaling pathways. However, an ever-increasing pool of evidences indicate that the delicate relationship between PCDs and the actin cytoskeleton is beginning to be elucidated. The purpose of this article is to review the current understanding of the relationships between different PCDs and the actin machinery (actin, actin-binding proteins and proteins involved in different actin signaling pathways), in the hope that this attempt can shed light on ensuing studies and the development of new therapeutic strategies.
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- 2021
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14. Blocking interaction between SHP2 and PD‐1 denotes a novel opportunity for developing PD‐1 inhibitors
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Zhenzhen Fan, Yahui Tian, Zhipeng Chen, Lu Liu, Qian Zhou, Jingjing He, James Coleman, Changjiang Dong, Nan Li, Junqi Huang, Chenqi Xu, Zhimin Zhang, Song Gao, Penghui Zhou, Ke Ding, and Liang Chen
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immunotherapy ,methylene blue ,PD‐1 ,small molecular inhibitor ,transgenic mouse model ,Medicine (General) ,R5-920 ,Genetics ,QH426-470 - Abstract
Abstract Small molecular PD‐1 inhibitors are lacking in current immuno‐oncology clinic. PD‐1/PD‐L1 antibody inhibitors currently approved for clinical usage block interaction between PD‐L1 and PD‐1 to enhance cytotoxicity of CD8+ cytotoxic T lymphocyte (CTL). Whether other steps along the PD‐1 signaling pathway can be targeted remains to be determined. Here, we report that methylene blue (MB), an FDA‐approved chemical for treating methemoglobinemia, potently inhibits PD‐1 signaling. MB enhances the cytotoxicity, activation, cell proliferation, and cytokine‐secreting activity of CTL inhibited by PD‐1. Mechanistically, MB blocks interaction between Y248‐phosphorylated immunoreceptor tyrosine‐based switch motif (ITSM) of human PD‐1 and SHP2. MB enables activated CTL to shrink PD‐L1 expressing tumor allografts and autochthonous lung cancers in a transgenic mouse model. MB also effectively counteracts the PD‐1 signaling on human T cells isolated from peripheral blood of healthy donors. Thus, we identify an FDA‐approved chemical capable of potently inhibiting the function of PD‐1. Equally important, our work sheds light on a novel strategy to develop inhibitors targeting PD‐1 signaling axis.
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- 2020
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15. Enhancing the Seismic Performance of Precast RC Frames with Cladding Panels through Setting U-Shaped Dampers and Rocking Walls
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Qing Jiang, Hanqin Wang, Yulong Feng, Xun Chong, Junqi Huang, and Yibo Liu
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Physics ,QC1-999 - Abstract
An approach combining U-shaped dampers (USDs) and rocking walls is proposed in this paper to improve the seismic performance of traditional precast reinforced concrete (RC) frames with cladding panels (PRCFCPs): (1) the steel bar and USD connection methods are adopted at the top and bottom of the cladding panels to use the relative deformation between the cladding panels and the main structure and then dissipate the seismic energy and (2) rocking walls are added to the structure to control the structural deformation profiles. The USD numerical model is calibrated using the test data, and a series of nonlinear pushover analyses, dynamic time-history analyses, and incremental dynamic analyses are successively performed to compare the seismic performance and collapse capacity of the PRCFCP, PRCFCP with USDs (PRCFCP-USD), and PRCFCP with USDs and rocking walls (PRCFCP-USD-RW).The results show that the USDs in the PRCFCP-USD-RW undergo more uniform deformation along the structural height and higher energy dissipation efficiency and the PRCFCP-USD-RW exhibits enhanced seismic performance and collapse capacity, which verify the superiority of the proposed combined approach.
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- 2020
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16. The multidisciplinary treatment of osteosarcoma of the proximal tibia: a retrospective study
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Junqi Huang, Wenzhi Bi, Gang Han, Jinpeng Jia, Meng Xu, and Wei Wang
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Osteosarcoma ,Tibia ,Arthroplasty ,Reconstruction ,Survival ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
Abstract Background Survival and reconstruction constitute important challenges in multimodal treatment of osteosarcoma of the proximal tibia. The purpose of this study was to assess the efficacy and prognosis of neoadjuvant chemotherapy and custom-designed endoprosthetic arthroplasty. Methods A total of 69 patients with osteosarcoma of the proximal tibia were evaluated, including 43 males and 26 females, treated with multidisciplinary limb-salvage remedy from October 2003 to December 2013. They were at least 12 years old (mean, 20 years; range, 12–57 years). The gap between tumor and main artery/nerve was showed in MRI. Mean follow up was 69.5 months (range, 9–144 months). Kaplan-Meier survival curves were generated to assess prognosis and relapse rate. The initial symptoms and disease duration for each patient were recorded. Correlation analyses were performed for the association of various parameters with prognosis. Functional outcomes were evaluated using the Musculoskeletal Tumor Society (MSTS) guidelines after 6 months postoperatively, to analyze the relation between bone excision size and function recovery. Results The resection lengths measured intraoperatively ranged from 80 to 230 mm, and contained 3 cm of normal bone around the tumor. A total of 3 courses of preoperative chemotherapy were administered to all cases. At final follow-up, 1 case showed recurrence. Meanwhile, 8 patients (11.6%) died from lung metastasis. Post-operative infection occurred in 3 patients; 1 case was maintained with revision surgery. Two cases underwent amputation. The mean MSTS system score was 21.6. Conclusions The multidisciplinary treatment result in an overall positive outcome, with improved function.
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- 2018
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17. Heart regeneration in adult Xenopus tropicalis after apical resection
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Souqi Liao, Wenyan Dong, Luocheng Lv, Hongyan Guo, Jifeng Yang, Hui Zhao, Ruijin Huang, Ziqiang Yuan, Yilin Chen, Shanshan Feng, Xin Zheng, Junqi Huang, Weihuan Huang, Xufeng Qi, and Dongqing Cai
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Xenopus tropicalis ,Regeneration of cardiomyocytes ,Heart injury and repair ,Scar-free ,Biotechnology ,TP248.13-248.65 ,Biology (General) ,QH301-705.5 ,Biochemistry ,QD415-436 - Abstract
Abstract Background Myocardium regeneration in adult mammals is very limited, but has enormous therapeutic potentials. However, we are far from complete understanding the cellular and molecular mechanisms by which heart tissue can regenerate. The full functional ability of amphibians to regenerate makes them powerful animal models for elucidating how damaged mature organs are naturally reconstituted in an adult organism. Like other amphibians, such as newts and axolotls, adult Xenopus displays high regenerative capacity such as retina. So far, whether the adult frog heart processes regenerative capacity after injury has not been well delineated. Results We examined the regeneration of adult cardiac tissues of Xenopus tropicalis after resection of heart apex. We showed, for the first time, that the adult X. tropicalis heart can regenerate perfectly in a nearly scar-free manner approximately 30 days after injury via apical resection. We observed that the injured heart was sealed through coagulation immediately after resection, which was followed by transient fibrous tissue production. Finally, the amputated area was regenerated by cardiomyocytes. During the regeneration process, the cardiomyocytes in the border area of the myocardium adjacent to the wound exhibited high proliferation after injury, thus contribute the newly formed heart tissue. Conclusions Establishing a cardiac regeneration model in adult X. tropicalis provides a powerful tool for recapitulating a perfect regeneration phenomenon and elucidating the underlying molecular mechanisms of cardiac regeneration in an adult heart, and findings from this model may be applicable in mammals.
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- 2017
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18. FgSfl1 and Its Conserved PKA Phosphorylation Sites Are Important for Conidiation, Sexual Reproduction, and Pathogenesis in Fusarium graminearum
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Chen Gong, Junqi Huang, Daiyuan Sun, Daiying Xu, Yuqian Guo, Jiangang Kang, Gang Niu, and Chenfang Wang
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protein kinase A ,spontaneous suppressors ,plant infection ,sexual reproduction ,phosphorylation ,Biology (General) ,QH301-705.5 - Abstract
The fungal plant pathogen, Fusarium graminearum, contains two genes, FgCPK1 and FgCPK2, encoding the catalytic subunits of cAMP-dependent protein kinase A. FgCPK1 and FgCPK2 are responsible for most of the PKA activities and have overlapping functions in various cellular processes in F. graminearum. The cpk1 cpk2 double mutant was significantly reduced in growth, rarely produced conidia, and was non-pathogenic. In this study, we found that the cpk1 cpk2 double mutant was unstable and produced fast-growing spontaneous sectors that were defective in plant infection. All spontaneous suppressor strains had mutations in FgSFL1, a transcription factor gene orthologous to SFL1 in yeast. Thirteen suppressor strains had non-sense mutations at Q501, three suppressor strains had frameshift mutations at W198, and five suppressor strains had mutations in the HSF binding domain of FgSfl1. Only one suppressor strain had both a non-synonymous mutation at H225 and a non-sense mutation at R490. We generated the SFL1 deletion mutant and found that it produced less than 2% of conidia than that of the wild-type strain PH-1. The sfl1 mutant was significantly reduced in the number of perithecia on carrot agar plates at 7 days post-fertilization (dpf). When incubated for more than 12 days, ascospore cirrhi were observed on the sfl1 mutant perithecia. The infection ability of the sfl1 deletion mutant was also obviously defective. Furthermore, we found that in addition to the S223 and S559 phosphorylation sites, FgSFL1 had another predicted phosphorylation site: T452. Interestingly, the S223 phosphorylation site was responsible for sexual reproduction, and the T452 phosphorylation site was responsible for growth and sexual reproduction. Only the S559 phosphorylation site was found to play an important role in conidiation, sexual reproduction, and infection. Overall, our results indicate that FgSFL1 and its conserved PKA phosphorylation sites are important for vegetative growth, conidiation, sexual reproduction, and pathogenesis in F. graminearum.
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- 2021
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19. Curvature-induced expulsion of actomyosin bundles during cytokinetic ring contraction
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Junqi Huang, Ting Gang Chew, Ying Gu, Saravanan Palani, Anton Kamnev, Douglas S Martin, Nicholas J Carter, Robert Anthony Cross, Snezhana Oliferenko, and Mohan K Balasubramanian
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cytokinesis ,Schizosaccharomyces pombe ,actomyosin ring ,curvature ,Schizosaccharomyces japonicus ,Medicine ,Science ,Biology (General) ,QH301-705.5 - Abstract
Many eukaryotes assemble a ring-shaped actomyosin network that contracts to drive cytokinesis. Unlike actomyosin in sarcomeres, which cycles through contraction and relaxation, the cytokinetic ring disassembles during contraction through an unknown mechanism. Here we find in Schizosaccharomyces japonicus and Schizosaccharomyces pombe that, during actomyosin ring contraction, actin filaments associated with actomyosin rings are expelled as micron-scale bundles containing multiple actomyosin ring proteins. Using functional isolated actomyosin rings we show that expulsion of actin bundles does not require continuous presence of cytoplasm. Strikingly, mechanical compression of actomyosin rings results in expulsion of bundles predominantly at regions of high curvature. Our work unprecedentedly reveals that the increased curvature of the ring itself promotes its disassembly. It is likely that such a curvature-induced mechanism may operate in disassembly of other contractile networks.
- Published
- 2016
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20. IFI6 Inhibits Apoptosis via Mitochondrial-Dependent Pathway in Dengue Virus 2 Infected Vascular Endothelial Cells.
- Author
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Yiming Qi, Ying Li, Yingke Zhang, Lin Zhang, Zilian Wang, Xuzhi Zhang, Lian Gui, and Junqi Huang
- Subjects
Medicine ,Science - Abstract
Dengue hemorrhagic fever (DHF)/Dengue shock syndrome (DSS) is a fatal infectious disease that demands an effective treatment. Interferon (IFN)-stimulated genes (ISGs) induced by dengue virus (DENV) exert antiviral effects. Among ISGs, IFN-α inducible gene 6 (IFI6) was increased in DENV infected human umbilical vascular endothelial cells (HUVECs) by microarray analysis in our previous study. However, its function is incompletely understood. In this study, we confirmed that IFI6 was markedly induced in DENV infection of both primary HUVECs and EA.hy926 cell lines. Recombinant EA.hy926 cell lines in which IFI6 was either over-expressed (IFI6+/+) or knocked-down (IFI6-/-) were generated. The activation of caspase-3 and intrinsic apoptosis-related protein caspase-9 were down-regulated in IFI6+/+ but up-regulated in IFI6-/- cells at 24-48 hrs post-infection. After incubation with DENV for 48 hrs, the mitochondrial membrane potential (Δψ(m)) was more stable in IFI6+/+ cells but reduced in IFI6-/- cells, as assayed by fluorescence staining with JC-1. We observed that Bcl-2 expression was increased in IFI6+/+ and decreased in IFI6-/- cells. By contrast, Bax expression was decreased in IFI6+/+ and increased in IFI6-/- cells. It is presumed that the anti-apoptotic function of IFI6 is expressed by regulating the rheostatic balance between bcl-2/bax expression and inhibition of Δψ(m) depolarization during DENV infection of vascular endothelial cells(VECs). In addition, the pro-apoptotic protein X-linked Inhibitor of Apoptosis (XIAP)-Associated Factor 1(XAF1) expression had been reported to be up-regulated and led to the induction of apoptosis in DENV2-infected VECs,but the relationship between XAF1 and IFI6 dengue virus-induced apoptosis in VECs warrants further study.
- Published
- 2015
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21. Correction: IFI6 Inhibits Apoptosis via Mitochondrial-Dependent Pathway in Dengue Virus 2 Infected Vascular Endothelial Cells.
- Author
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Yiming Qi, Ying Li, Yingke Zhang, Lin Zhang, Zilian Wang, Xuzhi Zhang, Lian Gui, and Junqi Huang
- Subjects
Medicine ,Science - Published
- 2015
- Full Text
- View/download PDF
22. Comparative brain stem lesions on MRI of acute disseminated encephalomyelitis, neuromyelitis optica, and multiple sclerosis.
- Author
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Zhengqi Lu, Bingjun Zhang, Wei Qiu, Zhuang Kang, Liping Shen, Youming Long, Junqi Huang, and Xueqiang Hu
- Subjects
Medicine ,Science - Abstract
BackgroundBrain stem lesions are common in patients with acute disseminated encephalomyelitis (ADEM), neuromyelitis optica (NMO), and multiple sclerosis (MS).ObjectivesTo investigate comparative brain stem lesions on magnetic resonance imaging (MRI) among adult patients with ADEM, NMO, and MS.MethodsSixty-five adult patients with ADEM (n = 17), NMO (n = 23), and MS (n = 25) who had brain stem lesions on MRI were enrolled. Morphological features of brain stem lesions among these diseases were assessed.ResultsPatients with ADEM had a higher frequency of midbrain lesions than did patients with NMO (94.1% vs. 17.4%, PConclusionsBrain stem lesions showed various morphological features among adult patients with ADEM, NMO, and MS. The different lesion locations may be helpful in distinguishing these diseases.
- Published
- 2011
- Full Text
- View/download PDF
23. An Inexact Newton Method For Unconstrained Total Variation-Based Image Denoising by Approximate Addition.
- Author
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Junqi Huang, Haider A. F. Almurib, T. Nandha Kumar, and Fabrizio Lombardi
- Published
- 2022
- Full Text
- View/download PDF
24. CrackF-Net: a pixel-level segmentation network for pavement crack detection.
- Author
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Shen Luan, Xingen Gao, Chen Wang, Hongyi Zhang, Fei Chao 0001, Juqiang Lin, Junqi Huang, Huali Jiang, and Feng Lin
- Published
- 2023
- Full Text
- View/download PDF
25. Commutative Approximate Adders: Analysis and Evaluation.
- Author
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Junqi Huang, T. Nandha Kumar, Haider A. F. Almurib, and Fabrizio Lombardi
- Published
- 2021
- Full Text
- View/download PDF
26. Generic Expressions for Early Estimation of Performance of Binary Multipliers.
- Author
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Junqi Huang, T. Nandha Kumar, and Haider Abbas F. Almurib
- Published
- 2021
- Full Text
- View/download PDF
27. A Deterministic Low-Complexity Approximate (Multiplier-Less) Technique for DCT Computation.
- Author
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Junqi Huang, T. Nandha Kumar, Haider A. F. Almurib, and Fabrizio Lombardi
- Published
- 2019
- Full Text
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28. Approximate computing using frequency upscaling.
- Author
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Junqi Huang, T. Nandha Kumar, Haider Abbas, and Fabrizio Lombardi
- Published
- 2019
- Full Text
- View/download PDF
29. Comparison Between NPV and IRR: Evaluation of Investment
- Author
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Junqi Huang
- Abstract
The net present value (NPV) and internal rate of return (IRR) are frequently used criteria for choosing capital investment projects which has been used for long days since they been created. But these two indicators may lead to contrary result in certain situations. This difference caused the debate that last more than a century about which investment deciding methods is better. Naturally, research on the inadequacy of these two methods has also continued for a long time and achieved many results such as a more reliable IRR calculation method - MIRR, the analysis of multiple roots in the IRR calculation in complex cases to determine the final result. This paper gives the brief definitions of NPV and IRR, and how they are calculated. The questions of when to use different investment decision-making methods is also answered in the paper. After that, the advantages and disadvantages of NPV and IRR were analyzed. At the same time, this paper has also fully researched the improvement of the deficiencies of two investment deciding methods. Finally, this paper compares NPV and IRR, and analyzes why these two methods lead to different results in some certain situations and which investment decision methods is relatively better in investment and draw the final conclusion.
- Published
- 2023
30. Structural performance of a façade precast concrete sandwich panel enabled by a bar-type basalt fiber-reinforced polymer connector
- Author
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Junqi Huang, Qing Jiang, Xun Chong, Xianguo Ye, and Caihua Liu
- Subjects
Architecture ,Civil and Structural Engineering - Published
- 2023
31. Seismic and collapse performance of a hybrid structure comprising steel frame with precast concrete shear walls and cladding panels
- Author
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Hanqin Wang, Qing Jiang, Xun Chong, Yulong Feng, and Junqi Huang
- Published
- 2022
32. Neoadjuvant Chemotherapy and Expandable Prosthesis Reconstruction to Treat Osteosarcoma around the Knee in Children
- Author
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Junqi Huang, Jiajia Cheng, Wenzhi Bi, Meng Xu, Jinpeng Jia, Gang Han, and Wei Wang
- Subjects
Orthopedics and Sports Medicine ,Surgery - Abstract
Survival and reconstruction in osteosarcoma is quite challenging. The study aimed to investigate the prognosis in patients treated with neoadjuvant chemotherapy and determine the clinical outcomes of expandable endoprosthesis reconstruction in children.From January 2009 to December 2014, we retrospectively analyzed 29 skeletally immature children (mean age, 10.5 years; range, 6-15 years) with osteosarcoma around the knee. Of the 29 patients who underwent neoadjuvant chemotherapy and limb salvage surgery, an expandable prosthesis was implanted for reconstruction. No patients were missed during follow-up. The evaluation index involved follow-up time, complication, functional results, and lengthening procedures. The survivorship and recurrence were assessed by GraphPad Software, and the function was evaluated by the Musculoskeletal Tumor Society (MSTS) scoring system.A mean follow-up time was 8.9 years (range, 6-12 years), and the overall 5-year survival was 89.1% based on Kaplan-Meier analysis. Three patients suffered a relapse and one underwent amputation. Lung metastasis developed in one patient. At 6 months after the operation, patients had a mean MSTS score of 27 points (range, 24-29). Two patients underwent revision surgery, one for implant infection and one for aseptic loosening. Prognosis is correlated with alkaline phosphatase change after treatment.Chemotherapy scheme and limb salvage can achieve high survival rates. This expandable prosthesis was associated with good function and low complication rates. The character of expandability could be a method to overcome discrepancies in the growth period.
- Published
- 2022
33. Out-of-plane flexural performance of concrete composite wide flat beam with hollow-core slabs
- Author
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Qing Jiang, Hanqin Wang, Yulong Feng, Xun Chong, Junqi Huang, Kun Zhang, and Fei Yu
- Subjects
Architecture ,Building and Construction ,Safety, Risk, Reliability and Quality ,Civil and Structural Engineering - Published
- 2022
34. Multi-branch power parameter monitoring system based on LoRa network
- Author
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Huali Jiang, Xingen Gao, JunQi Huang, Weishi Shi, Hongyi Shen, and Hang Chen
- Published
- 2023
35. Ferroptosis is determined by chloride ions
- Author
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Dichun Huang, Zhangshuai Dai, Chao Wang, Haiying Mai, Junyi Zhao, Lingbo Cao, Sijie Wang, Zhun Dai, Kun Xu, Ronghan He, Shaoshen Zhu, Yanchen Chen, Tiejun Bing, Xinzhou Zhu, Ting Gang Chew, and Junqi Huang
- Abstract
Ferroptosis has emerged as a deliberate type of programmed cell death that manifests marked importance ubiquitously in health and diseases. However, after a decade of research, the mechanisms of ferroptosis execution remain unclear. Here we identify chloride ions (Cl-) as essential determinants of ferroptosis. Water homeostasis manipulated by extracellular solute concentration disrupts ferroptotic cell death. Hyperosmotic stress attenuates ferroptosis and endues cells with high lipid peroxidation. Analyses of a fluorescent chloride probe show that Cl-fluxes into the cytoplasm during ferroptosis, substantiating a role for Cl-to drive water flow. Depletion of extracellular chloride ions ([Cl-]o) from culture media congruously confers resistance to ferroptosis. The [Cl-]o-depleted ferroptotic cells fall into two populations: cells with low lipid peroxidation; cells with high lipid peroxidation but not cell swelling or cell rupture. Contrarily, solitary [Cl-]ooverload is sufficient to elicit ferroptosis without canonical ferroptosis inducers. Further experiments show that ferroptotic cells depolarize and [Cl-]ois positively correlated with this process. Membrane depolarization upregulates the level of lipid peroxidation, suggesting that membrane potential may be a universal mechanism governing ferroptosis. Together, our findings reveal that ferroptosis is determined by chloride ions.
- Published
- 2023
36. Analytical Modeling of Solute Transport in Groundwater: Using Models to Understand the Effect of Natural Processes on Contaminant Fate and Transport
- Author
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Mark Goltz, Junqi Huang and Mark Goltz, Junqi Huang
- Published
- 2017
37. Axial compression performance of multi-cell concrete filled steel tubular flat columns
- Author
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Hanqin Wang, Qing Jiang, Junqi Huang, Xun Chong, Dianchun Xuan, and Zhipeng Huang
- Subjects
Mechanics of Materials ,Architecture ,Building and Construction ,Safety, Risk, Reliability and Quality ,Civil and Structural Engineering - Published
- 2023
38. Dynamic evolution of ceftazidime--avibactam resistance due to interchanges between blaKPC-2 and blaKPC-145 during treatment of Klebsiella pneumoniae infection.
- Author
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Yili Chen, Runshi Yang, Penghao Guo, Pingjuan Liu, Jiankai Deng, Zhongwen Wu, Qingping Wu, Junqi Huang, and Kang Liao
- Subjects
KLEBSIELLA infections ,KLEBSIELLA pneumoniae ,CARBAPENEM-resistant bacteria ,CEFTAZIDIME ,MICROBIAL sensitivity tests ,FOSFOMYCIN - Abstract
Background: The emergence of ceftazidime--avibactam(CZA) resistance among carbapenem-resistant Klebsiella pneumoniae (CRKP) is of major concern due to limited therapeutic options. Methods: In this study, 10 CRKP strains were isolated from different samples of a patient with CRKP infection receiving CZA treatment. Whole-genome sequencing (WGS) and conjugation experiments were performed to determine the transferability of the carbapenem resistance gene. Results: This infection began with a KPC-2-producing K. pneumoniae (CZA MIC = 2 mg/mL, imipenem MIC ≥ 16 mg/mL). After 20 days of CZA treatment, the strains switched to the amino acid substitution of T263A caused by a novel KPC- producing gene, blaKPC-145, which restored carbapenem susceptibility but showed CZA resistance (CZA MIC ≥ 256 mg/mL, imipenem MIC = 1 mg/mL). The bla
KPC-145 gene was located on a 148,185-bp untransformable IncFII-type plasmid. The subsequent use of carbapenem against KPC-145-producing K. pneumoniae infection led to a reversion of KPC-2 production (CZA MIC = 2 mg/ mL, imipenem MIC ≥ 16 mg/mL). WGS analysis showed that all isolates belonged to ST11-KL47, and the number of SNPs was 14. This implied that these blaKPC- positive K. pneumoniae isolates might originate from a single clone and have been colonized for a long time during the 120-day treatment period. Conclusion: This is the first report of CZA resistance caused by blaKPC-145 , which emerged during the treatment with CZA against blaKPC-2 -positive K. pneumoniae-associated infection in China. These findings indicated that routine testing for antibiotic susceptibility and carbapenemase genotype is essential during CZA treatment. [ABSTRACT FROM AUTHOR]- Published
- 2023
- Full Text
- View/download PDF
39. On the Commutative Operation of Approximate CMOS Ripple Carry Adders (RCAs)
- Author
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Fabrizio Lombardi, Haider Almurib, Nandha Kumar Thulasiraman, and Junqi Huang
- Abstract
Approximate cells can be used to design Ripple Carry Adders (RCAs) for realizing approximate addition in energy-efficient CMOS digital circuits. As inputs of approximate cells could be non-commutative in nature, approximate adders may show different output values under a commutative operation, and this may have a significant effect on the generated sum. This paper presents a detailed analysis of the commutative addition in RCAs made of different approximate cells. Initially, the impact of a non-commutative addition (NCA) to RCAs by approximate cells is assessed by exhaustive simulation at adder level. The results show that at most 17% of additions executed using AFA3 suffer from the non-commutative property, while the values for other adder cells can reach 75%~99%. Then, an extensive analysis using images from a publicly available library is performed by comparing three-image additions with two-image additions. As a further evaluation, the adders are assessed in an image denoising application. As expected, the effect of NCA is especially pronounced for some non-commutative adders, such as AA2 and AMA4. NCA is also cumulative with the number of approximate additions, thereby causing a significant variation in the output image quality. In terms of metrics, the largest average difference in mean error distance (DMED) for three-image addition is 5.3 times higher than for two-image addition. Rankings of the non-commutative approximate adders show that AMA3 and AFA1 based adders are the best schemes with respect to commutative addition; they both also show good performance in image denoising.
- Published
- 2022
40. Myosin turnover controls actomyosin contractile instability
- Author
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Sathish Thiyagarajan, Shuyuan Wang, Ting Gang Chew, Junqi Huang, Lokesh Kumar, Mohan K. Balasubramanian, and Ben O’Shaughnessy
- Subjects
Myosin Type II ,Actin Cytoskeleton ,Cytoskeletal Proteins ,Multidisciplinary ,Actomyosin ,Myosins ,Actins ,Cytokinesis - Abstract
Actomyosin contractile force produced by myosin II molecules that bind and pull actin filaments is harnessed for diverse functions, from cell division by the cytokinetic contractile ring to morphogenesis driven by supracellular actomyosin networks during development. However, actomyosin contractility is intrinsically unstable to self-reinforcing spatial variations that may destroy the actomyosin architecture if unopposed. How cells control this threat is not established, and while large myosin fluctuations and punctateness are widely reported, the full course of the instability in cells has not been observed. Here, we observed the instability run its full course in isolated cytokinetic contractile rings in cell ghosts where component turnover processes are absent. Unprotected by turnover, myosin II merged hierarchically into aggregates with increasing amounts of myosin and increasing separation, up to a maximum separation. Molecularly explicit simulations reproduced the hierarchical aggregation which precipitated tension loss and ring fracture and identified the maximum separation as the length of actin filaments mediating mechanical communication between aggregates. In the final simulated dead-end state, aggregates were morphologically quiescent, including asters with polarity-sorted actin, similar to the dead-end state observed in actomyosin systems in vitro. Our results suggest the myosin II turnover time controls actomyosin contractile instability in normal cells, long enough for aggregation to build robust aggregates but sufficiently short to intercept catastrophic hierarchical aggregation and fracture.
- Published
- 2022
41. A temporally relaxed theory of physically or chemically non-equilibrium solute transport in heterogeneous porous media
- Author
-
Ying-Fan Lin, Junqi Huang, Elliot J. Carr, Tung-Chou Hsieh, Hongbin Zhan, and Hwa-Lung Yu
- Subjects
Water Science and Technology - Published
- 2023
42. Molecular landscape of IDH-mutant primary astrocytoma Grade IV/glioblastomas
- Author
-
Zhen-Yu Zhang, Hong Chen, Aden Ka-Yin Chan, Houtan Noushmehr, Ho Keung Ng, Gabriel Chun-Hei Wong, Queenie Hoi-Wing Wong, Xianzhi Liu, Danny Tat Ming Chan, Kay Ka-Wai Li, Johnny S. H. Kwan, Weiwei Wang, Wencai Li, Tathiane M. Malta, Yura Grabovska, Chris Jones, Queenie Junqi Huang, Ying Mao, and Zhifeng Shi
- Subjects
Adult ,Male ,0301 basic medicine ,Pathology ,medicine.medical_specialty ,DNA Mutational Analysis ,Astrocytoma ,Biology ,medicine.disease_cause ,Subclass ,Pathology and Forensic Medicine ,Pathogenesis ,03 medical and health sciences ,0302 clinical medicine ,CDKN2A ,medicine ,Humans ,neoplasms ,Gene ,ATRX ,Mutation ,Brain Neoplasms ,Middle Aged ,Prognosis ,medicine.disease ,Isocitrate Dehydrogenase ,030104 developmental biology ,030220 oncology & carcinogenesis ,Cancer research ,GLIOMA ,Female ,DNA mismatch repair ,Glioblastoma - Abstract
WHO 2016 classified glioblastomas into IDH-mutant and IDH-wildtype with the former having a better prognosis but there was no study on IDH-mutant primary glioblastomas only, as previous series included secondary glioblastomas. We recruited a series of 67 IDH-mutant primary glioblastomas/astrocytoma IV without a prior low-grade astrocytoma and examined them using DNA-methylation profiling, targeted sequencing, RNA sequencing and TERT promoter sequencing, and correlated the molecular findings with clinical parameters. The median OS of 39.4 months of 64 cases and PFS of 25.9 months of 57 cases were better than the survival data of IDH-wildtype glioblastomas and IDH-mutant secondary glioblastomas retrieved from datasets. The molecular features often seen in glioblastomas, such as EGFR amplification, combined +7/-10, and TERT promoter mutations were only observed in 6/53 (11.3%), 4/53 (7.5%), and 2/67 (3.0%) cases, respectively, and gene fusions were found only in two cases. The main mechanism for telomere maintenance appeared to be alternative lengthening of telomeres as ATRX mutation was found in 34/53 (64.2%) cases. In t-SNE analyses of DNA-methylation profiles, with an exceptional of one case, a majority of our cases clustered to IDH-mutant high-grade astrocytoma subclass (40/53; 75.5%) and the rest to IDH-mutant astrocytoma subclass (12/53; 22.6%). The latter was also enriched with G-CIMP high cases (12/12; 100%). G-CIMP-high status and MGMT promoter methylation were independent good prognosticators for OS (p = 0.022 and p = 0.002, respectively) and TP53 mutation was an independent poor prognosticator (p = 0.013) when correlated with other clinical parameters. Homozygous deletion of CDKN2A/B was not correlated with OS (p = 0.197) and PFS (p = 0.278). PDGFRA amplification or mutation was found in 16/59 (27.1%) of cases and was correlated with G-CIMP-low status (p = 0.010). Aside from the three well-known pathways of pathogenesis in glioblastomas, chromatin modifying and mismatch repair pathways were common aberrations (88.7% and 20.8%, respectively), the former due to high frequency of ATRX involvement. We conclude that IDH-mutant primary glioblastomas have better prognosis than secondary glioblastomas and have major molecular differences from other commoner glioblastomas. G-CIMP subgroups, MGMT promoter methylation, and TP53 mutation are useful prognostic adjuncts.
- Published
- 2021
43. Experimental investigation on flexural performance of semi‐precast reinforced concrete one‐way slab with joint
- Author
-
Xianguo Ye, Junqi Huang, Yun-Lin Liu, and Xun Chong
- Subjects
Flexural strength ,Mechanics of Materials ,business.industry ,Precast concrete ,Slab ,General Materials Science ,Joint (building) ,Building and Construction ,Structural engineering ,Reinforced concrete ,business ,Geology ,Civil and Structural Engineering - Published
- 2021
44. Structural performance and section optimization of precast concrete sandwich panels with pin-type GFRP connectors
- Author
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Chao-Liang Zhao, Wang Ziyang, Qing Jiang, Junqi Huang, and Xun Chong
- Subjects
Materials science ,business.industry ,0211 other engineering and technologies ,Glass fiber reinforced polymer ,020101 civil engineering ,02 engineering and technology ,Building and Construction ,Structural engineering ,Sandwich panel ,Fibre-reinforced plastic ,0201 civil engineering ,Section (archaeology) ,Precast concrete ,021105 building & construction ,Facade ,business ,Sandwich-structured composite ,Civil and Structural Engineering - Abstract
A precast concrete sandwich panel (PCSP) offers a good potential in the application of façade wall due to the improved energy efficiency. In this study, the structural performance of PCSP with pin-type glass fiber reinforced polymer (GFRP) connectors was investigated, and an optimization characterized by ribbed structural wythe was proposed and studied. Firstly, the pull-out and shear capacity of the pin-type connector were evaluated through direct tensile test and direct shear test, respectively. Thereafter, seven PCSP specimens were fabricated and tested under four points flexural load. The investigating parameters included the structural wythe thickness, loading direction, insulation bond, and section type of the structural wythe. The load-deflection relationship, crack pattern, failure mode, load-strain relationship, and degree of composite action of the PCSP were studied and compared. It was concluded that: (1) the tested PCSPs presented ductile failures; (2) the structural wythe thickness, loading direction and insulation bond would influence the cracking, yielding, and peak loads of the tested PCSP; (3) the PCSP with pin-type GFRP connectors could be designed as non-composite type owing to the low composite action; and (4) the proposed ribbed structural wythe could achieve a lightweight PCSP while considerable flexural stiffness and capacity could be retained.
- Published
- 2021
45. Quasi-static and fatigue testing of earthquake-resilient steel joints with replaceable buckling-restrained links
- Author
-
Xun Chong, Liu Yibo, Feng Yulong, Yi Zhu, Junqi Huang, and Qing Jiang
- Subjects
Materials science ,business.industry ,0211 other engineering and technologies ,020101 civil engineering ,02 engineering and technology ,Building and Construction ,Structural engineering ,Dissipation ,Compression (physics) ,0201 civil engineering ,Hysteresis ,Buckling ,021105 building & construction ,Architecture ,Resilience (materials science) ,Safety, Risk, Reliability and Quality ,business ,Joint (geology) ,Beam (structure) ,Quasistatic process ,Civil and Structural Engineering - Abstract
This paper introduces the buckling-restrained concept into the design of traditional steel beam-column joints to improve the seismic and resilience performance. The upper and lower flanges of the beam are replaced by two buckling-restrained flanges (BRFs) to form a novel earthquake-resilient steel joint with a replaceable buckling-restrained link (RBRL). Four quasi-static tests and one fatigue test were conducted with various loading protocols, joint forms and core plate restraint systems. The experimental results, including the test phenomena, hysteresis curves (and the corresponding skeleton curves, energy dissipation capacity curves and stiffness degradation curves), and displacements and strains at the main positions of the specimens were obtained and analyzed. The test results show that the proposed joints exhibit an excellent performance in terms of hysteresis, fatigue and resilience, as expected. Comparative analysis results show that the loading protocols have an insignificant influence on the hysteresis curves before fracturing occurs. Additionally, the joint with a reduced section link is prone to instability and shows a lower energy dissipation capacity than the joint with the proposed RBRL, and when the compression gap in the restraint system is placed at the end of the core plate, the proposed joint behaves better.
- Published
- 2021
46. Investigation of flexural performance and effective width of composite wide flat beams
- Author
-
Qing Jiang, Fei Yu, Yulong Feng, Xun Chong, Junqi Huang, Kun Zhang, and Hanqin Wang
- Subjects
Civil and Structural Engineering - Published
- 2023
47. In situ bioremediation of perchlorate-contaminated groundwater using a multi-objective parallel evolutionary algorithm.
- Author
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Mark R. Knarr, Mark N. Goltz, Gary B. Lamont, and Junqi Huang
- Published
- 2003
- Full Text
- View/download PDF
48. Does supply-demand law work for ICBC's stock price?
- Author
-
Yue Qi, Junqi Huang, and Xiaofeng Peng
- Published
- 2013
- Full Text
- View/download PDF
49. Clinical and mutational profiles of adult medulloblastoma groups
- Author
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Anthony P. Y. Liu, Aden Ka-Yin Chan, Queenie Junqi Huang, Weiwei Wang, Queenie Hoi-Wing Wong, Xianzhi Liu, Ying Mao, Hong Chen, Nellie Yuk Fei Chung, Zhen-Yu Zhang, Zhifeng Shi, Danny Tat Ming Chan, Manix Fung-Man Poon, Kay Ka-Wai Li, Ho Keung Ng, and Gabriel Chun-Hei Wong
- Subjects
Adult ,Male ,Adult Medulloblastoma ,Adolescent ,MYC ,lcsh:RC346-429 ,Pathology and Forensic Medicine ,Metastasis ,Cellular and Molecular Neuroscience ,symbols.namesake ,Young Adult ,Transcription Factor 4 ,medicine ,Humans ,TP53 ,Cerebellar Neoplasms ,Telomerase ,Wnt Signaling Pathway ,lcsh:Neurology. Diseases of the nervous system ,Proportional Hazards Models ,Sanger sequencing ,business.industry ,Adult medulloblastoma ,Large cell ,Research ,Wnt signaling pathway ,KMT2C ,TCF4 ,Middle Aged ,medicine.disease ,Prognosis ,Molecular group ,Neoplasm Proteins ,DNA-Binding Proteins ,Patched-1 Receptor ,Survival Rate ,PTCH1 ,Mutation ,Cancer research ,symbols ,Targeted sequencing ,Female ,Neurology (clinical) ,DDX3X ,business ,Medulloblastoma - Abstract
Adult medulloblastomas are clinically and molecularly understudied due to their rarity. We performed molecular grouping, targeted sequencing, and TERT promoter Sanger sequencing on a cohort of 99 adult medulloblastomas. SHH made up 50% of the cohort, whereas Group 3 (13%) was present in comparable proportion to WNT (19%) and Group 4 (18%). In contrast to paediatric medulloblastomas, molecular groups had no prognostic impact in our adult cohort (p = 0.877). Most frequently mutated genes were TERT (including promoter mutations, mutated in 36% cases), chromatin modifiers KMT2D (31%) and KMT2C (30%), TCF4 (31%), PTCH1 (27%) and DDX3X (24%). Adult WNT patients showed enrichment of TP53 mutations (6/15 WNT cases), and 3/6 TP53-mutant WNT tumours were of large cell/anaplastic histology. Adult SHH medulloblastomas had frequent upstream pathway alterations (PTCH1 and SMO mutations) and few downstream alterations (SUFU mutations, MYCN amplifications). TERT promoter mutations were found in 72% of adult SHH patients, and were restricted to this group. Adult Group 3 tumours lacked hallmark MYC amplifications, but had recurrent mutations in KBTBD4 and NOTCH1. Adult Group 4 tumours harboured recurrent mutations in TCF4 and chromatin modifier genes. Overall, amplifications of MYC and MYCN were rare (3%). Since molecular groups were not prognostic, alternative prognostic markers are needed for adult medulloblastoma. KMT2C mutations were frequently found across molecular groups and were associated with poor survival (p = 0.002). Multivariate analysis identified histological type (p = 0.026), metastasis (p = 0.031) and KMT2C mutational status (p = 0.046) as independent prognosticators in our cohort. In summary, we identified distinct clinical and mutational characteristics of adult medulloblastomas that will inform their risk stratification and treatment.
- Published
- 2020
50. Pedicle screw-rod fixation and pelvic external fixation for the treatment of unstable pelvic ring fracture
- Author
-
Junqi Huang, Jiajia Cheng, Tao Wang, Bo Shi, Dingwei Zhang, and Nan Ye
- Abstract
Background Pelvic fracture is often associated with life-threatening damage and mechanical instability. Surgical therapy is a prior choice. To minimize surgical invasion and risk, bilateral screws combined with curved rod were applied to stabilize posterior pelvic ring. Anterior external fixation was to improve the fixation strength. This study was aim to explore the clinical effect of the procedure. Methods From January 2018 to January 2020, 27 patients with posterior pelvic fracture were included retrospectively. There were 12 males and 15 females with an average age of 56.3 ± 14.2 years. The prognosis of pelvis was evaluated by Matta and Majeed scores. Relevant clinical evaluation indications include the time of fracture healing, limb function and complications. Results The average follow-up time was 14.2 ± 5.4 month. Matta scoring standard: excellent in 18 cases, good in 7 cases, the good rate was 92.6%. The average healing time was 8.4 months. The standard of Majeed score in 6 months after operation: excellent in 14 cases, good in 10 cases, the good rate was 88.8%. At the last follow-up, the functional recovery of the affected limb was satisfactory. No deep infection occurred after operation.Conclusion The results indicate that screw-rod system and external fixation is a safe technique. Minimally invasive technology reduced frequency of fluoroscopy. It provides a more simple and safety method for posterior pelvic fracture failed conservative treatment.
- Published
- 2022
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