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Inspired by “crimped” collagen fiber, which induce a low-level load region within the hierarchical structures of ligaments and tendons, this study introduces a braiding technology aiming at characterizing nonlinear mechanical behavior. Samples of the developed artificial ligament with a multilayer braided structure were fabricated and tested. Alterations in the number of braiding layers, wales, and courses significantly affect the mechanical properties of the artificial ligaments. Specifically, the toe length exhibited an increase of up to 104.58 %, while the linear stiffness exhibited variations concomitant with changes in these parameters. Additionally, a mathematical model to describe the relationship between the toe length and linear stiffness was developed based on the braiding parameters and material properties of the fibers. Alternative fibers were employed and tested to demonstrate a consistency with the mathematical model. The correlation coefficients were calculated as 0.9644 for the toe length and 0.9203 for the linear stiffness. Lastly, this study mimics the mechanical behavior of the human medial collateral ligament (MCL) by integrating the artificial ligament into a bio-fidelity knee model with a joint stiffness of 1.77 Nm/deg, resulting in a high degree of similarity with human knee in valgus-varus, further affirming the utility and applicability of this study.