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Additional file 3: Table S1. Primers to amplify T. cruzi FeSOD-A and FeSOD-B genes. Abbreviations: Temp, Predicted annealing temperature. Figure S3. PCR amplification products of FeSOD-A and FeSOD-B genes of 8 T. cruzi strains. Electrophoresis was performed in a 1% agarose gel stained with GelRed™ (Biotium). The molecular weight markers 1-kb Plus DNA Ladder (Invitrogen) were used. Abbreviations: NC, Negative control of reaction without template. Table S2. Data of read depth and coverage analysis. The depth read values and coverage proportion in the genomic regions (GenBank: MZ825448–MZ825457) for each FeSOD type of genes and the respective normalized value (gene/genome depth). Depth was calculated using the value for each position of the gene. The coverage corresponds to the proportion of position that has reads in the gene, and normalized values are the depth of the genes divided by the mean of genome depth. Table S3. Sequence assembly information. Reads refers to the number of reads used for sequence assembly; Consensus refers to the length (nt) of the consensus sequence. Sequence assembly was performed using Phred-Phrap-Consed.

Additional file 8: Table S5.In vitro trypanocidal activity, cytotoxicity and selectivity index of selected drugs. Superscripts: 1Reduction (%) of the amastigote and trypomastigote by drug; 2drug concentration that inhibits 50% of the growth of the amastigotes and trypomastigotes of T. cruz;3drug concentration that inhibits 50% of the L929 cell viability;4CC50 L929/IC50 T. cruz. IC50 and CC50 values were calculated by linear interpolation. The determination of CC50 of polaprezinc was not possible due low solubility in concentrations > 50 μM. Abbreviations: ND, Not determined. Fig. S7. The diagram illustrates FeSOD-A, FeSOD-B and FeSOD-C characteristics, including cellular localization (orange boxes), protein length (blue boxes), gene copies (green boxes) and protein domains (pink boxes). Information was retrieved from public databases and tools: ENA, Pfam, TriTrypDB, and UniProt. Reference sequences were selected for FeSOD-A (AAC47548.2), FeSOD-B (EAN92179.1) and FeSOD-C (TcCLB.511735.60). Only gene copy numbers were obtained by our prediction using the T. cruzi CL-Brener genome.

Endophytic fungi represent ubiquitous microbial organisms able to live in the tissues of different plants around the world and represent a prolific source of bioactive metabolites. In the present study, the endophytic fungus Aspergillus calidoustus was isolated from the medicinal plant Acanthospermum australe (Asteraceae), and identified using molecular, physiological and morphological methods. A methylene chloride crude extract of A. calidoustus has been produced and subjected to antifungal bioassay-directed fractionation which resulted in the isolation of the two bioactive compounds: ophiobolin K and 6-epi-ophiobolin K. These pure compounds displayed antifungal activity against fungal plant pathogens, protozoal activity against Trypanosoma cruzi, and cytotoxic activity against human tumoral cell lines. The results show that A. calidoustus was able to produce the antifungal and cytotoxic metabolites ophiobolin K and 6-epi-ophiobolin K, which may help the fungus to colonise and occupy the substratum as well as survive in natural environments.