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Malignant transformation of chronic burn scars are usually toward cutaneous lineages, including squamous cell carcinoma, basal cell carcinoma, and malignant melanoma. Sarcomas are less common. Undifferentiated pleomorphic sarcoma(UPS) is a subtype of soft tissue sarcoma with storiform-pleomorphic cells of uncertain origin, and has sparingly been reported to arise from burn scars. The majority are localized lesions probably due to the spatial distance of mesenchymal cells from the epithelium. The authors describe a rare case of UPS of the chronic burn scar of his knee with ipsilateral femoral and external iliac lymph node metastasis. [ABSTRACT FROM AUTHOR]

The morphology of lettuce roots in hydroponics is affected by the flow environment of nutrient solutions. Regulating the flow rate therefore ensures quality and yield; however, the mechanisms underlying this impact have not been fully elucidated, and breakthroughs in regulatory methods are thus lacking. Herein, lettuce was grown in a hydroponic solution at three different flow rates, the root morphology indicators (such as root length and surface area) were evaluated, the hormone levels of roots grown under different flow conditions were measured, and the correlations between plant hormones and root morphology were analyzed. Transcriptomic analysis was conducted on roots at varying flow rates to investigate the differential expression of genes involved in the synthesis and signal transduction pathways of auxin, abscisic acid (ABA), ethylene, and jasmonic acid. Results showed that these plant hormones were closely related to root morphology. Compared to a non-flow environment, a flow environment promoted the synthesis of plant hormones in the roots and enhanced the signal transduction of certain hormones, such as auxin. However, it had a negative impact on the signal transduction of certain hormones, specifically ethylene. The flow of the nutrients regulated root morphogenesis by influencing the synthesis and transduction of plant hormones, which affected overall plant growth. During the same growth period, lettuce grown under suitable flow conditions had a higher fresh weight than lettuce grown under no-flow conditions. Although such flow would increase the electricity cost of operating water pumps, lettuce could be harvested in advance at appropriate flow rates, thereby reducing the energy consumed in plant factories through lighting and temperature regulation, which are the largest contributors to energy consumption. This study examined the impact of the nutrient solution flow environment as a positive stimulus for plant growth, and it provides a reference for strategically regulating the flow rate in plant factories to improve yield and save energy. Future research should explore the effects of other cultivation and flow methods on the growth of different plants and apply these findings to practical production.

Root morphology, an important determinant of nutrient absorption and plant growth, can adapt to various growth environments to promote survival. Solution flow under hydroponic conditions provides a mechanical stimulus, triggering adaptive biological responses, including altered root morphology and enhanced root growth and surface area to facilitate nutrient absorption. To clarify these mechanisms, we applied untargeted metabolomics technology, detecting 1737 substances in lettuce root samples under different flow rates, including 17 common differential metabolites. The abscisic acid metabolic pathway product dihydrophaseic acid and the amino and nucleotide sugar metabolism factor N-acetyl-d-mannosamine suggest that nutrient solution flow rate affects root organic acid and sugar metabolism to regulate root growth. Spatial metabolomics analysis of the most stressed root bases revealed significantly enriched Kyoto Encyclopedia of Genes and Genomes pathways: “biosynthesis of cofactors” and “amino sugar and nucleotide sugar metabolism”. Colocalization analysis of pathway metabolites revealed a flow-dependent spatial distribution, with higher flavin mononucleotide, adenosine-5′-diphosphate, hydrogenobyrinic acid, and D-glucosamine 6-phosphate under flow conditions, the latter two showing downstream-side enrichment. In contrast, phosphoenolpyruvate, 1-phospho-alpha-D-galacturonic acid, 3-hydroxyanthranilic acid, and N-acetyl-D-galactosamine were more abundant under no-flow conditions, with the latter two concentrated on the upstream side. As metabolite distribution is associated with function, observing their spatial distribution in the basal roots will provide a more comprehensive understanding of how metabolites influence plant morphology and response to environmental changes than what is currently available in the literature.

The principal difference between hydroponics and other substrate cultivation methods is the flowing liquid hydroponic cultivation substrate. Our previous studies have revealed that a suitable flowing environment of nutrient solution promoted root development and plant growth, while an excess flow environment was unfavorable for plants. To explain the thigmomorphogenetic response of excess flow-induced metabolic changes, six groups of lettuce (Lactuca sativa L.), including two flow conditions and three time periods, were grown. Compared with the plants without flow, the plants with flow showed decreased root fresh weight, total root length, root surface area, and root volume but increased average root diameter and root density. The roots with flow had more upregulated metabolites than those without flow, suggesting that the flow may trigger metabolic synthesis and activity. Seventy-nine common differential metabolites among six groups were screened, and enrichment analysis showed the most significant enrichment in the arginine biosynthesis pathway. Arginine was present in all the groups and exhibited greater concentrations in roots with flow than without flow. It can be speculated from the results that a high-flowing environment of nutrient solution promotes arginine synthesis, resulting in changes in root morphology. The findings provide insights on root thigmomorphogenesis affected by its growing conditions and help understand how plants respond to environmental mechanical forces.

Crop models have been developed for wide research purposes and scales, but they have low compatibility due to the diversity of current modeling studies. Improving model adaptability can lead to model integration. Since deep neural networks have no conventional modeling parameters, diverse input and output combinations are possible depending on model training. Despite these advantages, no process-based crop model has been tested in full deep neural network complexes. The objective of this study was to develop a process-based deep learning model for hydroponic sweet peppers. Attention mechanism and multitask learning were selected to process distinct growth factors from the environment sequence. The algorithms were modified to be suitable for the regression task of growth simulation. Cultivations were conducted twice a year for 2 years in greenhouses. The developed crop model, DeepCrop, recorded the highest modeling efficiency (= 0.76) and the lowest normalized mean squared error (= 0.18) compared to accessible crop models in the evaluation with unseen data. The t-distributed stochastic neighbor embedding distribution and the attention weights supported that DeepCrop could be analyzed in terms of cognitive ability. With the high adaptability of DeepCrop, the developed model can replace the existing crop models as a versatile tool that would reveal entangled agricultural systems with analysis of complicated information.

Ultraviolet-B (UV-B, 280–315 nm) radiation has been known as an elicitor to enhance bioactive compound contents in plants. However, unpredictable yield is an obstacle to the application of UV-B radiation to controlled environments such as plant factories. A typical three-dimensional (3D) plant structure causes uneven UV-B exposure with leaf position and age-dependent sensitivity to UV-B radiation. The purpose of this study was to develop a model for predicting phenolic accumulation in kale (Brassica oleracea L. var. acephala) according to UV-B radiation interception and growth stage. The plants grown under a plant factory module were exposed to UV-B radiation from UV-B light-emitting diodes with a peak at 310 nm for 6 or 12 h at 23, 30, and 38 days after transplanting. The spatial distribution of UV-B radiation interception in the plants was quantified using ray-tracing simulation with a 3D-scanned plant model. Total phenolic content (TPC), total flavonoid content (TFC), total anthocyanin content (TAC), UV-B absorbing pigment content (UAPC), and the antioxidant capacity were significantly higher in UV-B-exposed leaves. Daily UV-B energy absorbed by leaves and developmental age was used to develop stepwise multiple linear regression models for the TPC, TFC, TAC, and UAPC at each growth stage. The newly developed models accurately predicted the TPC, TFC, TAC, and UAPC in individual leaves with R2 > 0.78 and normalized root mean squared errors of approximately 30% in test data, across the three growth stages. The UV-B energy yields for TPC, TFC, and TAC were the highest in the intermediate leaves, while those for UAPC were the highest in young leaves at the last stage. To the best of our knowledge, this study proposed the first statistical models for estimating UV-B-induced phenolic contents in plant structure. These results provided the fundamental data and models required for the optimization process. This approach can save the experimental time and cost required to optimize the control of UV-B radiation.

Supplemental interlighting is commonly used in modern greenhouses to improve light deficiency, but the light spectrum affects fruit quality and color change. This study aimed to analyze the effect of interlighting with red, blue, and additional far-red light on the fruit qualities and carotenoid contents of red and yellow sweet peppers (Capsicum annuum L.). Three light treatments were applied: natural light (NL), NL with red + blue LED interlighting (71 μmol m–2 s–1) (RB), and RB with far-red light (55 μmol m–2 s–1) (RBFR). Ascorbic acid, free sugars, and individual carotenoid content were quantified with HPLC analysis. Fruits were sampled on 2020.11.14 (Group 1) and 2021.01.03 (Group 2) from the plants grown under average light intensities of 335.9 and 105.6 μmol m–2 s–1, respectively. In the overall period, total yields in RB and RBFR were 22 and 33% higher than those in NL in red fruits and 2 and 21% higher in yellow fruits, respectively. In both colored fruits, ascorbic acid, total soluble sugar, and carotenoid content were higher in RB and RBFR than NL. In Group 1, ascorbic acid and total soluble sugar were significantly different between RB and RBFR only in red fruits. In Group 2, ascorbic acids in red and yellow fruits were 9 and 3% higher in RBFR than RB but total soluble sugars were 4 and 2% lower, respectively. Carotenoid contents in red and yellow fruits were 3.0- and 2.1-fold higher in RB and 2.0- and 1.4-fold higher in RBFR than those in NL, respectively. In this study, interlighting had a significant impact on fruit quality in Group 2, mainly due to the increase in the ratio of interlighting to total light by seasonal changes. In particular, red and yellow fruit yields were 9% and 19% higher in RBFR than RB, but carotenoid contents were 26 to 9% lower, respectively. This result exhibited that additional far-red lighting has a trade-off relationship between fruit yield and carotenoid content. Thus, it is necessary to provide an adequate light spectrum according to a specific cultivation purpose, such as improving yield or accumulating plastids in fruits.

Crop fresh weight and leaf area are considered non-destructive growth factors due to their direct relation to vegetative growth and carbon assimilation. Several methods to measure these parameters have been introduced; however, measuring these parameters using the existing methods can be difficult. Therefore, a non-destructive measurement method with high versatility is essential. The objective of this study was to establish a non-destructive monitoring system for estimating the fresh weight and leaf area of trellised crops. The data were collected from a greenhouse with sweet peppers (Capsicum annuum var. annuum); the target growth factors were the crop fresh weight and leaf area. The crop fresh weight was estimated based on the total system weight and volumetric water content using a simple formula. The leaf area was estimated using top-view images of the crops and a convolutional neural network (ConvNet). The estimated crop fresh weight and leaf area exhibited average R2 values of 0.70 and 0.95, respectively. The simple calculation was able to avoid overfitting with fewer limitations compared with the previous study. ConvNet was able to analyze raw images and evaluate the leaf area without additional sensors and features. As the simple calculation and ConvNet could adequately estimate the target growth factors, the monitoring system can be used for data collection in practice owing to its versatility. Therefore, the proposed monitoring system can be widely applied for diverse data analyses.

UV-B (280–315 nm) radiation has been used as an effective tool to improve bioactive compound contents in controlled environments, such as plant factories. However, plant structure changes with growth progress induce different positional distributions of UV-B radiation interception, which cause difficulty in accurately evaluating the effects of UV-B on biosynthesis of bioactive compounds. The objective of this study was to quantitatively analyze the positional distributions of UV-B radiation interception and bioactive compound contents of kales (Brassica oleracea L. var. acephala) with growth progress and their relationships. Short-term moderate UV-B levels did not affect the plant growth and photosynthetic parameters. Spatial UV-B radiation interception was analyzed quantitatively by using 3D-scanned plant models and ray-tracing simulations. As growth progressed, the differences in absorbed UV-B energy between leaf positions were more pronounced. The concentrations of total phenolic compound (TPC) and total flavonoid compound (TFC) were higher with more cumulative absorbed UV-B energy. The cumulative UV energy yields for TFC were highest for the upper leaves of the older plants, while those for TPC were highest in the middle leaves of the younger plants. Despite the same UV-B levels, the UV-B radiation interception and UV-B susceptibility in the plants varied with leaf position and growth stage, which induced the different biosynthesis of TFC and TPC. This attempt to quantify the relationship between UV-B radiation interception and bioactive compound contents will contribute to the estimation and production of bioactive compounds in plant factories.

Abstract Background Root-zone environment is considered difficult to analyze, particularly in interpreting interactions between environment and plant. Closed-loop soilless cultures have been introduced to prevent environmental pollution, but difficulties in managing nutrients can cause nutrient imbalances with an adverse effect on crop growth. Recently, deep learning has been used to draw meaningful results from nonlinear data and long short-term memory (LSTM) is showing state-of-the-art results in analyzing time-series data. Therefore the macronutrient ion concentrations affected by accumulated environment conditions can be analyzed using LSTM. Results The trained LSTM can estimate macronutrient ion concentrations in closed-loop soilless cultures using environmental and growth data. The average training accuracy of six macronutrients was R2 = 0.84 and the test accuracy was R2 = 0.67 with RMSE = 1.48 meq L−1. The used values of input interval and time step were 1 h and 168 (1 week), respectively. The accuracy was improved when the input interval became shorter, but not improved when the LSTM consisted of a multilayer structure. Regarding training methods, the LSTM improved the accuracy better than the non-LSTM. The trained LSTM showed relatively adequate accuracies and the interpolated ion concentrations showed variations similar to those seen during traditional cultivation. Conclusions We could analyze the nutrient balance in the closed-loop soilless culture, the model showed potential in estimating the macronutrient ion concentrations using environmental and growth factors measured in greenhouses. Since the LSTM is a powerful and flexible tool used to interpret accumulative changes, it is easily applicable to various plant and cultivation conditions. In the future, this approach can be used to analyze interactions between plant physiology and root-zone environment.

An electrical conductivity (EC)-based closed-loop soilless culture system is practical for in-field deployment. Literature on the closed-loop soilless culture nutrient management premise the limitations in managing recycled nutrients under dynamic changes in individual nutrient uptake concentrations. However, recent systems analysis studies predicting solutions for nutrient fluctuation stabilization in EC-based closed-loop soilless culture systems suggest that the system may have a deterministic side in nutrient variation. This study aims to derive a nutrient control principle in an EC-based nutrient recycling soilless culture system by theoretical and experimental analyses. An integrated model of solutes such as K+, Ca2+, and Mg2+ and water transport in growing media, automated nutrient solution preparation, and nutrient uptake was designed. In the simulation, the intrinsic characteristics of nutrient changes among open-, semi- closed-, and closed-loop soilless cultures were compared, and stochastic simulations for nutrient control were performed in the closed-loop system. Four automated irrigation modules for comparing nutrient changes among the soilless culture systems were constructed in the greenhouse. Sweet pepper plants were used in the experiment. In the experimental analysis, nutrient concentration conversion to the proportion between nutrients revealed distinctive trends of nutrient changes according to the treatment level of drainage recycling. Theoretical and experimental analyses exhibited that nutrient variations in open-, semi- closed-, and closed-loop soilless culture systems can be integrated as a function of nutrient supply to the system’s boundary areas. Furthermore, stochastic simulation analysis indicated that the nutrient ratio in the soilless culture system reveals the nutrient uptake parameter-based deterministic patterns. Thus, the nutrient ratio in the closed-loop soilless culture could be controlled by the long-term feedback of this ratio. We expect that these findings provide theoretical frameworks for systemizing nutrient management techniques in EC-based closed-loop soilless culture systems.

Ultraviolet-B (UV-B) acts as a regulatory stimulus, inducing the dose-dependent biosynthesis of phenolic compounds such as flavonoids at the leaf level. However, the heterogeneity of biosynthesis activation generated within a whole plant is not fully understood until now and cannot be interpreted without quantification of UV-B radiation interception. In this study, we analyzed the spatial UV-B radiation interception of kales (Brassica oleracea L. var. Acephala) grown under supplemental UV-B LED using ray-tracing simulation with 3-dimension-scanned models and leaf optical properties. The UV-B-induced phenolic compounds and flavonoids accumulated more, with higher UV-B interception and younger leaves. To distinguish the effects of UV-B energy and leaf developmental age, the contents were regressed separately and simultaneously. The effect of intercepted UV-B on flavonoid content was 4.9-fold that of leaf age, but the effects on phenolic compound biosynthesis were similar. This study confirmed the feasibility and relevance of UV-B radiation interception analysis and paves the way to explore the physical and physiological base determining the intraindividual distribution of phenolic compound in controlled environments.

Greenhouses require accurate and reliable data to interpret the microclimate and maximize resource use efficiency. However, greenhouse conditions are harsh for electrical sensors collecting environmental data. Convolutional neural networks (ConvNets) enable complex interpretation by multiplying the input data. The objective of this study was to impute missing tabular data collected from several greenhouses using a ConvNet architecture called U-Net. Various data-loss conditions with errors in individual sensors and in all sensors were assumed. The U-Net with a screen size of 50 exhibited the highest coefficient of determination values and the lowest root-mean-square errors for all environmental factors used in this study. U-Net50 correctly learned the changing patterns of the greenhouse environment from the training dataset. Therefore, the U-Net architecture can be used for the imputation of tabular data in greenhouses if the model is correctly trained. Growers can secure data integrity with imputed data, which could increase crop productivity and quality in greenhouses.

In plant factories, light is fully controllable for crop production but involves a cost. For efficient lighting, light use efficiency (LUE) should be considered as part of light environment design. The objectives of this study were to evaluate and interpret the light interception, photosynthetic rate, and LUE of lettuces under electrical lights using ray-tracing simulation. The crop architecture model was constructed by 3D scanning, and ray-tracing simulation was used to interpret light interception and photosynthesis. For evaluation of simulation reliability, measured light intensities and photosynthetic rates in a growth chamber were compared with those obtained by simulation at different planting densities. Under several scenarios modeling various factors affecting light environments, changes in light interception and LUE were interpreted. The light intensities and photosynthetic rates obtained by simulation showed good agreement with the measured values, with R2 > 0.86. With decreasing planting density, the light interception of the central plant increased by approximately 18.7%, but that of neighboring plants decreased by approximately 5.5%. Under the various scenarios, shorter lighting distances induced more heterogenetic light distribution on plants and caused lower light interception. Under a homogenous light distribution, the light intensity was optimal at approximately 360 μmol m−2 s−1 with an LUE of 6.5 g MJ−1. The results of this study can provide conceptual insights into the design of light environments in plant factories.

Although plant responses to artificial lighting spectra often produce abnormal morphogenesis and reduced productivity, no quantification method to determine how plants perceive and respond to light has been available. Our objective in this study was to test whether a plant’s spectral perception can be quantified using the light absorption of its major photoreceptors, phytochrome, cryptochrome, and phototropin. We developed an artificial solar lamp and three different light sources, based on a high-pressure sodium lamp, a fluorescent lamp, and red and blue light-emitting diodes, whose absorption by photoreceptors was equal to that of the standard solar spectrum. Cucumber plants grown under the artificial solar and developed light sources showed normal photomorphogenesis and were indistinguishable from each other. Plants grown under unmodified commercial light sources had abnormal photomorphogenesis that made them short and small. The photosynthetic rate was higher under the unmodified light sources; however, dry masses were highest under the artificial solar and modified light sources, indicating that the cucumber plants are optimized to the solar spectrum. Our results clearly demonstrate that the spectral perceptions of plants can be quantified using the light absorption of their photoreceptors, not visual color or spectra. We expect that our findings will contribute to a better understanding of plant perceptions of and responses to light quality, and improve the productivity of plants cultivated under artificial light.

Among abiotic stresses, both drought and UV-B radiation effectively trigger the accumulation of secondary metabolites, and can be widely applied in plant factories. The objectives of this study were to investigate antioxidant accumulation under drought stress alone, or in combination with UV-B radiation near harvest, and to determine an optimal treatment time for maximum antioxidant production. Kale (Brassica oleracea L. var. acephala) plants were grown in a plant factory and harvested at 42 days after transplanting. The single and combination treatments lasted for 7 to 1 days and 4 to 2 days before harvest, respectively. The results of both Fv/Fm (maximal photochemical efficiency in photosystem II) and leaf water potential could ensure the function of photosynthesis and maintain normal leaf moisture in single drought treatments of less than 4 days. The total phenolic and flavonoid contents and antioxidant activities were significantly increased in both single and combination treatments for 3 to 4 days, compared to other treatments. The supplementary UV-B treatments showed no extra formation of antioxidants compared to the single drought treatments. As a result, drought for 3 days before harvest could achieve the highest potential value of kale as a source of natural antioxidants.

Light is a major environmental factor affecting the regulation of secondary metabolites, such as pigments and flavor. The Solanaceae plant family has diverse patterns of fruit metabolisms that serve as suitable models to understand the molecular basis of its regulation across species. To investigate light-dependent regulation for fruit pigmentation and volatile flavors, major fruit pigments, their biosynthetic gene expression, and volatiles were analyzed in covered fruits of tomato and bell pepper. Immature covered fruits were found to be ivory in color and no chlorophyll was detected in both plants. The total carotenoid content was found to be reduced in ripe tomato and bell pepper under cover. Naringenin chalcone decreased more than 7-fold in ripe tomato and total flavonoids decreased about 10-fold in immature and ripe pepper fruit under light deficiency. Light positively impacts fruit pigmentation in tomato and bell pepper by regulating gene expression in carotenoid and flavonoid biosynthesis, especially phytoene synthase and chalcone synthase, respectively. Nineteen volatile flavors were detected, and seven of these exhibited light-dependent regulations for both ripe tomato and pepper. This study will help in improving fruit quality and aid future research works to understand the molecular mechanisms regulating the influence of light-dependency on pigments and flavor volatiles.

In existing closed-loop soilless cultures, nutrient solutions are controlled by the electrical conductivity (EC) of the solution. However, the EC of nutrient solutions is affected by both growth environments and crop growth, so it is hard to predict the EC of nutrient solution. The objective of this study was to predict the EC of root-zone nutrient solutions in closed-loop soilless cultures using recurrent neural network (RNN). In a test greenhouse with sweet peppers (Capsicum annuum L.), data were measured every 10 s from October 15 to December 31, 2014. Mean values for every hour were analyzed. Validation accuracy (R2) of a single-layer long short-term memory (LSTM) was 0.92 and root-mean-square error (RMSE) was 0.07, which were the best results among the different RNNs. The trained LSTM predicted the substrate EC accurately at all ranges. Test accuracy (R2) was 0.72 and RMSE was 0.08, which were lower than values for the validation. Deep learning algorithms were more accurate when more data were added for training. The addition of other environmental factors or plant growth data would improve model robustness. A trained LSTM can control the nutrient solutions in closed-loop soilless cultures based on predicted future EC. Therefore, the algorithm can make a planned management of nutrient solutions possible, reducing resource waste.

Fresh weight is a direct index of crop growth. It is difficult to continuously measure the fresh weight of bell peppers grown in soilless cultures, however, due to the difficulty in identifying the moisture condition of crops and growing media. The objective of this study was to develop a continuous and nondestructive measuring system for the fresh weight of bell peppers grown in soilless cultures considering the moisture content of growing media. The system simultaneously measures the trellis string’s supported weight and gravitational weight using tensile load cells. The moisture weight of growing media was calibrated during the growth period using changes in moisture content before and after the first irrigation of the day. The most stable time period for the measurement, from 03:00 to 06:00, was determined by analyzing the diurnal change in relative water content. To verify the accuracy of the system, the fruits, stems, leaves, and roots’ fresh weights were measured manually. The fresh weights measured by the developed system were in good agreement with those manually measured. The results confirm that our system can reliably and accurately measure fresh weights of bell peppers grown in soilless cultures. This method can be applied to continuous growth data collection for other crops grown in soilless cultures.

In closed-loop soilless culture systems, variation in nutrients can lead to instability in the nutrient management and forced discharge of nutrients and water. Total nutrients absorbed by plants are replenished in an electrical conductivity-based closed-loop system, and fluctuation in electrical conductivity within a certain range around the initial value can be expected. However, this is not always observed in systems using conventional nutrient-replenishment methods. The objectives of this study were to analyze nutrient variation in a closed-loop soilless culture system based on a theoretical model and derive an alternative nutrient-replenishment method. The performance of the derived alternative method was compared with a conventional nutrient-replenishment method through simulation analysis. A demonstration experiment using sweet peppers was then conducted to confirm whether the theoretical analysis results can be reproduced through actual cultivation. The average amounts of injected nutrients during the experimental period of four months in the conventional and alternative methods were 2257 and 1054 g, respectively. There was no significant difference in the yield of sweet peppers between the two methods. The substrate electrical conductivity in the alternative method was maintained at 2.7 dS⋅m−1 ± 0.5 within the target electrical conductivity value, while that in the conventional method gradually increased to 5.0 dS⋅m−1 ± 1.2. In a simulation study, results similar to the demonstration experiment were predicted. Total nutrient concentrations in the alternative method showed fluctuations around the target value but did not continuously deviate from the target value, while those in the conventional method showed a tendency to increase. As a whole, these characteristics of the alternative method can help in minimizing nutrients and water emissions from the cultivation system.

Canopy photosynthesis has typically been estimated using mathematical models that have the following assumptions: the light interception inside the canopy exponentially declines with the canopy depth, and the photosynthetic capacity is affected by light interception as a result of acclimation. However, in actual situations, light interception in the canopy is quite heterogenous depending on environmental factors such as the location, microclimate, leaf area index, and canopy architecture. It is important to apply these factors in an analysis. The objective of the current study is to estimate the canopy photosynthesis of paprika (Capsicum annuum L.) with an analysis of by simulating the intercepted irradiation of the canopy using a 3D ray-tracing and photosynthetic capacity in each layer. By inputting the structural data of an actual plant, the 3D architecture of paprika was reconstructed using graphic software (Houdini FX, FX, Canada). The light curves and A/Ci curve of each layer were measured to parameterize the Farquhar, von Caemmerer and Berry (FvCB) model. The difference in photosynthetic capacity within the canopy was observed. With the intercepted irradiation data and photosynthetic parameters of each layer, the values of an entire plant’s photosynthesis rate were estimated by integrating the calculated photosynthesis rate at each layer. The estimated photosynthesis rate of an entire plant showed good agreement with the measured plant using a closed chamber for validation. From the results, this method was considered as a reliable tool to predict canopy photosynthesis using light interception, and can be extended to analyze the canopy photosynthesis in actual greenhouse conditions.

Purpose: We report two siblings with genetically confirmed Walker-Warburg syndrome (WWS), studied with multimodal imaging, who presented with different retinal manifestations., Methods: This is a retrospective report of two WWS cases with ultra-widefield fundus photography, fluorescein angiography, and ultrasound. Molecular diagnosis was achieved using panel testing and targeted variant testing., Results: Two siblings, one male and one female, born 17 months apart with a diagnosis of WWS underwent retinal examination with imaging. The 3-month-old female infant exhibited microphthalmia, persistent hyaloidal arteries, and retrolental membranes with total tractional retinal detachments on ultrasound in both eyes. The 22-day-old male newborn exhibited persistent hyaloidal arteries and extensive peripheral avascular retina on angiography in both eyes. Both were found to be positive for the same two pathogenic variants in the RXYLT1/TMEM5 gene, which accounts for approximately 9% of cases of genetically confirmed WWS., Conclusion: Siblings with genetically confirmed WWS can have variable presentations despite identical genotype. This highlights the phenotypic disease spectrum of WWS, which may be similar to that seen in familial exudative vitreoretinopathy.

Psychostimulant drugs increase behavioral, cardiac and brain responses in humans and other animals. Acute food deprivation or chronic food restriction potentiates the stimulatory effects of abused drugs and increases the propensity for relapse to drug seeking in drug-experienced animals. The mechanisms by which hunger affects cardiac and behavioral activities are only beginning to be elucidated. Moreover, changes in motor neuron activities at the single neuron level induced by psychostimulants, and their modulation by food restriction, remain unknown. Here we investigated how food deprivation affects responses to d-amphetamine by measuring locomotor activity, cardiac output, and individual motor neuron activity in zebrafish larvae. We used wild-type larval zebrafish to record behavioral and cardiac responses and the larvae of Tg(mnx1:GCaMP5) transgenic zebrafish to record motor neuron responses. Physiological state gated responses to d-amphetamine. That is, d-amphetamine evoked significant increases in motor behavior (swimming distances), heart rate and motor neuron firing frequency in food-deprived but not fed zebrafish larvae. The results extend the finding that signals arising from food deprivation are a key potentiator of the drug responses induced by d-amphetamine to the zebrafish model. The larval zebrafish is an ideal model to further elucidate this interaction and identify key neuronal substrates that may increase vulnerability to drug reinforcement, drug-seeking and relapse., Competing Interests: Declaration of Competing Interest The authors have no conflict of interest to declare., (Copyright © 2023 Elsevier Inc. All rights reserved.)