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Drought and cold stresses seriously affect tree growth and fruit yield during apple (Malus domestica) production, with combined stress causing injury such as shoot shriveling. However, the molecular mechanism underlying crosstalk between responses to drought and cold stress remains to be clarified. In this study, we characterized the zinc finger transcription factor ZINC FINGER OF ARABIDOPSIS THALIANA 10 (ZAT10) through comparative analysis of shoot-shriveling tolerance between tolerant and sensitive apple rootstocks. MhZAT10 responded to both drought and cold stresses. Heterologous expression of MhZAT10 in the sensitive rootstock ‘G935' from domesticated apple (Malus domestica) promoted shoot-shriveling tolerance, while silencing of MhZAT10 expression in the tolerant rootstock ‘SH6' of Malus honanensis reduced stress tolerance. We determined that the apple transcription factor DEHYDRATION RESPONSE ELEMENT-BINDING PROTEIN 2A (DREB2A) is a direct regulator activating the expression of MhZAT10 in response to drought stress. Apple plants overexpressing both MhDREB2A and MhZAT10 genes exhibited enhanced tolerance to drought and cold stress, while plants overexpressing MhDREB2A but with silenced expression of MhZAT10 showed reduced tolerance, suggesting a critical role of MhDREB2A-MhZAT10 in the crosstalk between drought and cold stress responses. We further identified drought-tolerant MhWRKY31 and cold-tolerant MhMYB88 and MhMYB124 as downstream regulatory target genes of MhZAT10. Our findings reveal a MhDREB2A-MhZAT10 module involved in crosstalk between drought and cold stress responses, which may have applications in apple rootstock breeding programs aimed at developing shoot-shriveling tolerance.

The Air Pollution Prevention and Control Action Plan and Three-year Plan on Defending the Blue Sky promulgated by the State Council of the People's Republic of China have played an important role in the overall improvement of air quality in China. However, few studies have evaluated the implementation effects of these two policies in Sichuan Basin and the new characteristics of PM

To investigate the PM

To explore the characteristics of water-soluble inorganic ions (WSIIs) in PM

To investigate the characteristics of carbonaceous species in PM

The water-soluble inorganic ions (WSIIs) in PM

The objective of this experiment is to probe into the influence of soil nutrient contents on fruit quality features of 'Fuji' apple, screen major soil nutrient factors affecting fruit quality features, clarify the suitable soil nutrient content for good fruit quality indicators, and to provide theoretical basis in reasonable orchard soil fertilizer application for improving fruit qua-lity in the Circum-Bohai and Loess Plateau apple production regions in China. The soil nutrient contents and 'Fuji' fruit qualities were respectively analyzed at 66 commercial 'Fuji' apple orchards standard rootstock in the Circum-Bohai and Loess Plateau apple production regions of 22 counties in China from 2010 to 2011. The partial least squares regression (PLS) was used to screen major soil nutrient elements affecting fruit quality, and to establish regression equation of relationship between fruit quality and major soil nutrition factors. Linear programming was used to obtain optimum proposals of soil nutrient elements for good apple quality. The results showed that alkaline hydrolytic N, available P, Ca, Fe, and Zn of soil were significantly higher while pH and available K were significantly lower in Circum-Bohai region than in Loess Plateau apple production region. Soluble solid content in Loess Plateau was higher than in Circum-Bohai; moreover, ratio of soluble solids content and titratable acidity was lower. The soil available B in the Circum-Bohai and Loess Plateau apple production regions had positive effect on fruit mass, and total N had negative effect on fruit firmness. The soluble solid contents in the Circum-Bohai were mainly negatively affected by total N and positively by available B, while positively affected by available Ca and negatively by alkaline hydrolytic N in the Loess Plateau region. The desired soil nutrients for good fruit qualities were high avai-lable B, pH and suitable available K in the Circum-Bohai, and low total N, high alkaline hydrolytic N, available K, Fe and suitable available Zn and B in the Loess Plateau region. It was the key technical measures in soil nutrient management for good fruit quality to increase available B and pH, adjust available K in the Circum-Bohai, and to enhance alkaline hydrolytic N, available K, Fe, and to decrease pH, and adjust available Zn and B in the Loess Plateau region.探讨环渤海湾和黄土高原两大苹果产区土壤养分对‘富士’苹果品质的影响,筛选不同产区影响果实品质特性的主要土壤养分因子,明确优质‘富士’苹果的土壤养分含量指标等,为两大苹果产区果园合理施肥、提高果实品质等提供理论依据.于2010—2011年分别在我国环渤海湾和黄土高原两大苹果产区各选择22个县,每个县3片果园,共计132个乔砧‘富士’苹果园,对每个果园的土壤养分含量和果实品质指标进行调查和分析,应用偏最小二乘回归方法筛选不同产区影响果实品质的主要土壤养分因子,并建立果实品质因素与土壤养分含量关系的回归方程,线性规划求解不同产区优质‘富士’苹果的土壤养分含量优化方案.结果表明: 环渤海湾产区的土壤碱解氮、有效磷、钙、铁和锌含量极显著高于黄土高原产区,而土壤pH,有效钾含量显著低于黄土高原产区;黄土高原产区的果实可溶性固形物含量显著高于环渤海湾产区,而固酸比显著低于环渤海湾产区.土壤有效硼含量对两产区果实单果质量影响的正效应最大,而土壤总氮与两产区果实硬度呈负效应;环渤海湾产区的果实可溶性固形物含量主要受土壤总氮和有效硼的负、正效应影响,黄土高原产区主要受土壤有效钙和碱解氮正、负效应的影响.环渤海湾产区优质乔砧‘富士’苹果的土壤养分含量需求为高的土壤有效硼和pH,适宜的土壤有效钾;黄土高原产区为低的土壤全氮,高的碱解氮、有效钾和有效铁,适宜的土壤有效锌和硼.环渤海湾产区优质乔砧‘富士’苹果的土壤养分管理技术措施为增加土壤有效硼含量和调高土壤pH、调整土壤有效钾含量;而黄土高原产区为提高土壤碱解氮、有效钾和有效铁含量,降低果园土壤pH,适当调整土壤有效锌和有效硼含量.

The microwave resonant cavity is the sensor of wetness measurement in medium perturbation method. Local eddy current and viscous dissipation of stream will cause the temperature difference of cavity, which led to the shift of resonant frequency affecting the stability of sensor. This paper calculates the temperature distribution of cavity under conditions that varieties of steam parameters and velocity of flow by flow-solid coupling method. The thermal deformation of different conditions of cavity is calculated. The influence of thermal deformation to the resonance frequency of resonant cavity is analyzed, according to the average value of node deformation of the cavity effective surface. The results show that the average temperature of cavity and thermal deformation increase with the steam parameter increase and the offset value of resonance frequency increases. The wetness measurement scheme of wet steam on the turbine exhaust needs to improve to reduce the effect of cavity body deformation.

Steam pressure and resonant frequency of microwave cavity are important measure parameters of microwave wetness measurement system. The measuring precision of pressure and frequency directly affect the accuracy of wetness measurement. This paper takes different pressure and wetness of steam as example to analyze the parameter uncertainty of measurement relationship and deduces standard uncertainty of wetness measurement. The system comprehensive uncertainty is identified, when the parameters measurement, cavity heat expansion, sampling error, sedimentary water film and so on are considered. The result shows that the system uncertainty is less than 0.004%. The system uncertainty introduced by pressure measurement is small and can be neglected, but the system uncertainty from frequency measurement has a great effect. The precisely measurement of resonance frequency is key to ensure the accuracy of the system.

The impact of conservation tillage practices on carbon sequestration has been of great interest in recent years. This experiment analyzed the organic carbon status of soils sampled at depth increments from 0 to 60 cm after 20 years in a purple paddy soil. The tillage experiment was established in the Key Field Station for Monitoring of Eco-Environment of Purple Soil of the Ministry of Agriculture of China, located in the farm of Southwest University (30°26′N, 106°26′E), Chongqing. In this paper, five tillage treatments including conventional tillage with rice only system (DP), conventional tillage with rotation of rice and rape system (SL), no-till and ridge culture with rotation of rice and rape system (LM), no-till and plain culture with rotation of rice and rape system (XM) and tillage and ridge culture with rotation of rice and rape system (LF) were selected as research objectives to measure SOC storage and stratification ratio of SOC (CSR). The SOC storage under different tillage systems was calculated based on an equivalent soil mass. The CSR can be used as an indicator of soil quality because surface organic matter is essential to erosion control, water infiltration, and the conservation of nutrients. Results showed that in soil under no-till SOC was concentrated near the surface, while in tilled soil SOC decreased equably with the increase of soil depth. The difference of SOC contents between the five tillage systems was the largest in the top soil and the lowest in the bottom soil. The order of SOC storage was LM (158.52 Mg C•ha-1) >DP (106.74 Mg C•ha-1) >XM (100.11 Mg C•ha-1) >LF (93.11 Mg C•ha-1) >SL (88.59 Mg C•ha-1), LM treatment was significantly higher than the other treatments. The CSR of 0-10/50-60 cm was 2.65, 2.70 and 2.14 under LM, XM and LF treatments, while 1.54 and 1.92 under DP and SL treatments. We considered CSR>2 indicate an improvement in soil quality produced by changing from tillage to no-tillage, as well as changing from plane to ridge. Overall, long-term LM treatment is a valid strategy for increasing SOC storage and improving soil quality in a purple paddy soil in Southwest China.

In order to study the composition and distribution of VOCs (Volatile Organic Compounds) in the atmosphere in the urban area of Beibei district, Chongqing, atmospheric samples were collected from March 2012 to February 2013 with special stainless steel cylinders, and analyzed with a three-stage preconcentration method coupled with GC-MS. 78 species of VOCs were detected in this study, of which there were 25 species of alkanes, 15 species of olefins, 28 species of aromatic hydrocarbons and 10 species of halogenated hydrocarbons. The results showed that the top seven species of VOCs according to the order of annual average concentration in the atmosphere of Beibei were: Dichloromethane (3. 08 x 10(-9) ) , Benzene (2. 09 x 10-9) , Isopentane (1. 85 x 10 -9) , Toluene (1. 51 x 10(-9)) , Propane (1. 51 x 10(-9)), m/p-xylene (1.43 x 10(-9)) and Styrene (1. 39 x 10-9). The concentration of TVOCs (Total Volatile Organic Compounds) in the atmosphere of Beibei was 33. 89 x 10 -9 during the measuring period, and the seasonal variation was obvious with the order of spring (42. 57 x 10 -9)autumn (33.89 x 10-9)winter (31.91 x 10 -9)summer (27.04 x 10(-9)). In the composition of TVOCs, alkanes and aromatic hydrocarbons provided the largest contribution to TVOCs (31.5% and 30.7% ) , followed by halogenated hydrocarbon, accounting for 27.4% , and the last one was olefins, with only 10.4%. By means of ozone formation potential, the analysis results showed that olefins and aromatic hydrocarbon compounds were the two important materials which made the biggest contribution to the formation of ozone in the atmosphere of Beibei. We further analyzed the sources of VOCs in atmosphere of Beibei by the method of Principal Component Analysis (PCA). Vehicle exhaust was the biggest source and its contribution to VOCs was 50. 41%. The calculated results with T/B value also confirmed that traffic was the biggest source contributing to the VOCs in atmosphere of Beibei.

To study the characteristics of atmospheric pollutants in the urban area of Beibei district of Chongqing, the concentrations of the atmospheric pollutants were measured by automatic on-line continuous monitoring equipments from Jan. 2012 to Feb. 2013. The results showed that the concentrations of the pollutants often exceeded the corresponding values of the new National Ambient Air Quality Standards (GB 3095-2012) except SO2. Of these pollutants, PM2.5 was the most serious in this area. The concentrations of the atmospheric pollutants had significant seasonal variation. The concentrations of O3 and O(x) were both the highest in summer and the lowest in winter. The average concentrations of O3 were (36.1 +/- 19.2), (48.8 +/- 32.6), (29.8 +/- 28.6) and (18.2 +/- 15.8) microg x m(-3), and the average O(x) concentrations were (77.6 +/- 20.6), (91.3 +/- 37.6), (77.5 +/- 30.6) and (69.4 +/- 18.2) microg x m(-3) in spring, summer, autumn and winter in 2012, respectively. The concentrations of NO(x) appeared higher in winter and lower in summer, the average concentrations of NO, NO2 and NO(x) were (11.8 +/- 9.4), (42.3 +/- 13.1) and (54.1 +/- 20.8) microg x m(-3) in spring, (8.2 +/- 4.9), (40.5 +/- 9.9) and (48.7 +/- 12.6) microg x m(-3) in summer, (20.7 +/-17.1), (47.2 +/- 14.1) and (67.9 +/- 25.5) microg x m(-3) in autumn, and (30.4 +/- 25.1), (51.2 +/- 15.9), (81.6 +/- 37.9) microg x m(-3) in winter. The concentrations of SO2 appeared higher in spring and winter, and lower in summer and autumn. The concentrations of SO2 were (50.5 +/- 23.3), (26.3 +/- 16.7), (38.8 +/- 18.4) and (53.7 +/- 23.4) microg x m(-3) in spring, summer, autumn and winter, respectively. The concentrations of PM2.5 appeared higher in winter and changed smoothly in other seasons, with the average concentration of (61.4 +/- 28.5), (68.1 +/- 32.5), (61.9 +/- 27.1) and (89.6 +/- 44.2) microg x m(-3) in spring, summer, autumn and winter, respectively. The curves of diurnal variations of O3, O(x), NO, NO(x) and SO2 all showed single peak. However, the time of the peak values varied for different pollutants, 16:00 for O3 and O(x), and 8:00-11:00 for NO, NO(x) and SO2. The diurnal variations of NO2 and PM2.5 were similar to the two peaks that appeared in the morning and at night, respectively. Moreover, the diurnal ranges of O3 and O(x) concentrations were much wider in summer, while the wider ranges were observed in winter for NO, NO2, NO(x), SO2 and PM2.5. There was no difference in the diurnal pattern of NO between weekends and weekdays, the concentrations of N2O in weekdays were much higher than those on weekends, but with O3 the situation was the opposite. Correlation analysis indicated that the O3 concentration was positively correlated with temperature and wind speed, while negatively correlated with relative humidity. However, the situation of NO(x) was the opposite. PM2.5 concentration was negatively correlated with temperature and wind speed, while positively correlated with relative humidity. SO2 concentration had different correlations with the meteorological parameters in different seasons. In addition, wind direction was an important factor affecting the concentrations of the atmospheric pollutants.

Volatile organic compounds (VOCs) play important roles in the atmosphere as precursors of secondary air pollutants. The regional background concentrations and variation characteristics of VOCs in the atmosphere of southwestern China were studied. Meanwhile, a receptor model based on principal component analysis (PCA) was used to identify the major sources of VOCs. Weekly samples were collected in 2007 in the Gongga Mountain base station and analyzed with a three-stage preconcentration method coupled with GC-MS. The annual mean concentration of TVOCs and NMHCs were 9.40 x 10(-9) +/- 4.55 x 10(-9) and 7.73 x 10(-9) +/- 4.43 x 10(-9), respectively. Aromatic hydrocarbons provided the largest contribution to TVOCs (37.3%), follow by alkanes (30.0%) and halogenated hydrocarbons (19.8%), the smallest contribution was from alkenes (12.9%). Three major sources were resolved by the receptor model, traffic sources, biogenic sources and combustion sources. The seasonal variation of TVOCs in this area was obviously, and the order was autumnwinterspringsummer. TVOCs concentration in autumn was very significantly higher than that in summer (P0.01). The seasonal variation of the four types of VOCs showed different characteristics due to the differences in photochemical properties. Isoprene emissions were from biogenic sources. Regression analysis revealed a good exponential relationship between the isoprene concentration and temperature. High temperatures increased the isoprene concentrations. However, the isoprene concentration remained constant when the ambient air temperature was below 20 degrees C. The TVOCs in Gongga Mountain were at a medium level comparing with the results of other regions, and there was a clear background station emission characteristic.

Using the static opaque chamber method, a field experiment, located in the Key Field Station for Monitoring of Eco-Environment of Purple Soil of the Ministry of Agriculture of China in the farm of Southwest University (30 degrees 26'N, 106 degrees 26'E) in Chongqing, was conducted in situ for one year to study the effect of different tillage systems on CH4 and N2O emission from ago-ecosystems in a purple paddy soil. In this paper, four tillage treatments including conventional tillage with rice only system (CT), conventional tillage with rotation of rice and rape system (CTR), no-till and plain culture with rotation of rice and rape system (NTP) and no-till and ridge culture with rotation of rice and rape system (NTR) were selected as research objectives. The results showed that the annual CH4 and N2O emissions were mainly occurred in the rice growing period, and were about 77.6% and 55.0% of the total annual of them emitted from this period. The total annual CH4 under CT was higher than that of other treatments. The annual average flux of CH4[CH4, mg x (m2 x h)(-1)] order was CT (2.96 +/- 0.04)NTR (1.83 +/- 0.21)NTP (1.42 +/- 0.01)CTR (0.96 +/- 0.09); the annual average flux of N2O[N2O, microg x (m2 x h)(-1)] order was CTR (123.6 +/- 47.1)NTR (115.2 +/- 22.1)NTP (100.5 +/- 25.8)CT (81.3 +/- 13.5), and the total annual N2O under CTR was higher than that of CT. The global warming potentials (GWPs) of CH4 and N2O emissions under different tillage-cropping systems were assessed in an integrated way. The results showed that the integrated GWPs of CH4 and N2O emission were in the following sequence: CTNTRNTPCTR, and CTR was the best treatment for decrease the integrated GWPs in this area.

Guangyangzaji (Guang Yang Miscellaneous Notes) are historical notes written by a scholar of the Qing dynasty: Liu Xian-ting. This book recorded much medical content of the people. Doing simple research and analysis on the name, physical nature and effectiveness of the Lobular Pyrolae, Kai Wood (Chinese pistache), Huanglian snakes (a snake habitat with Coptis grass) and other native herbs can contribute to understanding the evolution of the medicinal herbs.

The physics of failure (PoF) methodology is a primary approach for prognostics, especially for those of electronic products and assemblies. This paper summarizes the requirements for developing a generic field deployable prognostic software system using the model-based approach. It then proposes an implementation solution to design such a system while meeting those requirements. It in the end shows a preliminary design of the system and presents some results that a prototype of the system delivers. This paper provides a technical framework for developing a model-based prognostic system for electronic products and assemblies.