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The rigid pile composite foundation is widely used in highway projects in soft soil area as it can effectively increase the bearing capacity and stability of the foundation. While the research on the behavior and failure mode of rigid pile composite foundation under embankment is not enough, instability failure of rigid pile composite foundation often occurs in practical projects. This paper presents a centrifuge model test to investigate the load transfer mechanism, settlement characteristic and failure mode of rigid pile composite foundation under embankment. The test results show that: the soil displacement of different region in rigid pile composite foundation was different, obvious vertical displacement occurred in the soil under the center of embankment and the horizontal displacement was very small in this region; both vertical and horizontal displacement occurred in the soil under the shoulder of embankment; and obvious horizontal displacement occurred in the soil under the slope toe of embankment; moreover, ground heave also occurred near the slope toe of embankment. The soil displacement in rigid pile composite foundation had a large influence on the stress characteristic and failure mode of rigid piles, the compressive failure and bending failure would probably occur for the piles under the center and shoulder of the embankment, respectively, and the tension-bending failure would probably occur for the piles under the slope toe of embankment. The different failure modes of piles at different regions should be considered in the design of rigid pile composite foundation under embankment. The test results can be used to improve the design method for rigid pile composite foundation under embankment in practical projects.

Estimated glomerular filtration rate (eGFR) has been reported to be associated with risk of incident coronary heart disease (CHD), and blood urea nitrogen (BUN) has been shown to be a strong predictor of mortality in patients with heart failure (HF). However, such epidemiological evidence from Chinese population was still limited. We used Cox proportional-hazards regression models to investigate the associations of eGFR and BUN with risk of incident CHD in the prospective Dongfeng-Tongji (DFTJ) cohort. After fully adjusted for potential confounders, a 10-unit decline in eGFR was associated with higher risk for CHD (hazard ratio [HR] 1.05, 95% confidence interval [CI] 1.01–1.09); compared with individuals with normal eGFR levels (eGFR ≥ 90 ml/min per 1.73 m2), individuals with a mild-to-severe eGFR decline (15 to 60 ml/min per 1.73 m2) were at significantly greater risk for CHD (HR 1.25, 95% CI 1.05–1.48; P = 0.011). Compared with individuals in the lowest tertile of BUN, those in the highest tertile were at significantly greater risk for CHD (HR 1.17, 95% CI 1.03–1.33; P = 0.014). In conclusion, a mild-to-severe decline in eGFR or a raised level of BUN might be associated with increased risk of incident CHD in middle-aged and elderly Chinese populations.

Alicella gigantea (Alicelloidae) is a scavenger with the largest body size among amphipods. It is a participant in the foodweb of deepsea ecosystem and distributed with vast bathymetric and geographic ranges. In this study, the mitochondrial genome of A. gigantea was completely assembled and characterized. The complete sequence has a total length of 16,851 bp, comprising the usual eukaryotic components, with 13 protein-coding genes (PCGs), 2 ribosomal RNA genes (rRNAs), 22 transfer RNA genes (tRNAs), and 2 noncoding control regions (CRs). The gene rearrangement and reverse nucleotide strand bias of its mitochondrial genome are similar to those observed in the deepsea amphipod Eurythenes maldoror (Eurytheneidae), but different from the characters of Halice sp. MT-2017 (Dexaminoidea), an inhabitant of a deeper environment. Phylogenetic analysis indicates that A. gigantea occupies the basal branch of deepsea species—E. maldoror and Hirondellea gigas. This phylogeny supports the hypothesis that the evolution of hadal amphipods has undergone a transition from the abyssal depth. Compared to 41 available shallow water equivalents, the four accessible mitochondrial genomes from the deep sea, including the one produced in this study, show significantly fewer charged amino acids in the 13 PCGs, which suggests an adaption to the deepsea environment.

Small amphipods (Halice sp. MT-2017) with body length Halice sp. shared the usual gene components of metazoans, comprising 13 protein coding genes (PCGs), 22 transfer RNAs (tRNAs), and 2 ribosomal RNAs (rRNAs). The arrangement of these genes, however, differed greatly from that of other amphipods. Of the 15 genes that were rearranged with respect to the pancrustacean gene pattern, 12 genes (2 PCGs, 2 rRNAs, and 8 tRNAs) were both translocated and strand-reversed. In contrast, the mitochondrial genomes in other amphipods never show so many reordered genes, and in most instances, only tRNAs were involved in strand-reversion-coupled translocation. Other characteristics, including reversed strand nucleotide composition bias, relatively higher composition of non-polar amino acids, and lower evolutionary rate, were also identified. Interestingly, the latter two features were shared with another hadal amphipod, Hirondellea gigas, suggesting their possible associations with the adaptation to deep-sea extreme habitats. Overall, our data provided a useful resource for future studies on the evolutionary and adaptive mechanisms of hadal faunas.

In this study, the complete mitochondrial DNA sequence of a hagfish Eptatretus sp. Nan-Hai from a depth of 1000 m is presented. The complete sequence was determined using next-generation sequencing and long PCRs. The mitochondrial genome of Eptatretus sp. Nan-Hai is 17,538 bps in size and composed of 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and one control region (D-loop). The base composition of mitochondrial genome is biased toward A + T content, at 67.21%, with GC skew of −0.35 and AT skew of −0.03. A phylogenetic tree revealed that within the genus Eptatretus, Eptatretus sp. Nan-Hai is closely related to Eptatretus atami.

The purpose of this study was to sonographically assess the cerebral hemodynamic differences and changes after oxygen therapy in healthy youths of different ethnicities in Tibet.Sixty-six healthy young Han visitors and 29 healthy young Tibetan residents were divided into 4 groups. Basic information was collected. Pulsed Doppler sonography was used to record the cerebral hemodynamic parameters for the internal carotid, vertebral, and middle cerebral arteries. The participants were then instructed to inhale oxygen, and basic information and cerebral hemodynamic parameters were recorded at 1, 2, 4, and 8 minutes, respectively. Differences in these parameters between groups were analyzed.In comparisons of the flow parameters between sex-matched groups, the mean resistive index values for the internal carotid, vertebral, and middle cerebral arteries in the Han groups were significantly lower than those in the Tibetan groups (P. 05). The mean peak systolic velocity, end-diastolic velocity, and mean velocity values for the middle cerebral artery in the Han groups were significantly higher than those in the Tibetan groups (P.05). After oxygen uptake, there were no significant differences in the mean arterial oxygen saturation, heart rate, mean velocity, and resistive index values between the male groups, and similar changes were found for the arterial oxygen saturation and peak systolic velocity values between female groups after 8 minutes of oxygen uptake (P.05).Sonography is a useful modality for noninvasive and real-time detection of changes in cerebral hemodynamics and can provide reference values for the prevention and treatment of cerebrovascular diseases.

Ontogeny reversal, as seen in some cnidarians, is an unprecedented phenomenon in the animal kingdom involving reversal of the ordinary life cycle. Three species of Turritopsis have been shown to be capable of inverted metamorphosis, a process in which the pelagic medusa transforms back into a juvenile benthic polyp stage when faced with adverse conditions. Turritopsis sp.5 is a species of Turritopsis collected from Xiamen, China which presents a similar ability, being able to reverse its life cycle if injured by mechanical stress. Phylogenetic analysis based on both 16S rDNA and cytochrome c oxidase subunit I (COI) genetic barcodes shows that Turritopsis sp.5 is phylogenetically clustered in a clade separate from other species of Turritopsis. The genetic distance between T. sp.5 and the Japanese species T. sp.2 is the shortest, when measured by the Kimura 2-Parameter metric, and the distance to the New Zealand species T. rubra is the largest. An experimental assay on the induction of reverse development in this species was initiated by cutting medusae into upper and lower parts. We show, for the first time, that the two dissected parts have significantly different potentials to transform into polyps. Also, a series of morphological changes of the reversed life cycle can be recognised, including medusa stage, contraction stage I, contraction stage II, cyst, cyst with stolons, and polyp. The discovery of species capable of reverse ontogeny caused by unfavorable conditions adds to the available systems with which to study the cell types that contribute to the developmental reversal and the molecular mechanisms of the directional determination of ontogeny.

Centrifuge model tests are carried out to study the composite foundation under embankment which is reinforced by rigid piles with caps on the layered ground that the upper layer was soft clay and the lower layer was stiff sand. Variable acceleration loading method, constant acceleration loading method and gasbag loading method are applied to simulate the construction process, operation process and failure process of embankment. Working behavior, which including displacement of embankment and ground, internal force and deformation of piles in different position, soil stress and pore water pressure between piles, and failure modes of composite foundation are analyzed. Results show that the ground deformation is presented as settlement, horizontal displacement and toe heave. The pile-soil stress ratio in the middle of embankment (25.0) is greater than that in slope shoulder (17.4). Failure modes of piles in different position under embankment are different. To the piles under the middle of embankment, the most likely failure mode is compression failure. The piles under slope shoulder may be subjected to press-bending failure or overturning failure, and the piles under slope toe may occur tension-bending failure or overturning failure.

Objective. The present study aimed to investigate the effect of fluoride-modified titanium surface on adhesion of irradiated osteoblasts.Materials and Methods. Fluoride-modified surface was obtained and the morphology, roughness, and chemical composition of the surface were evaluated by scanning electron microscopy, atomic force microscopy, and X-ray photoelectron spectroscopy, respectively. The adhesion of irradiated osteoblast-like cells, in terms of number, area, and fluorescence intensity on the titanium surface, was evaluated using immunofluorescence staining.Results. Numerous nanosize pits were seen only in the F-TiO surface. The pits were more remarkable and uniform on F-TiO surface than on TiO surface; however, the amplitude of peaks and bottoms on F-TiO surface appeared to be smaller than on TiO surface. The Sa value and Sdr percentage of TiO surfaces were significantly higher than those of F-TiO surface. The concentrations of main elements such as titanium, oxygen, and carbon were similar on both surfaces. The number of irradiated osteoblasts adhered on the control surface was larger than on fluoride-modified surface. Meanwhile, the cells on the fluoride-modified surface formed more actin filaments.Conclusions. The fluoride-modified titanium surface alters the adhesion of irradiated osteoblasts. Further studies are needed to investigate the proliferation, differentiation, maturation, gene expression, and cytokine production of irradiated osteoblasts on fluoride-modified titanium surface.

The gut microbiota is contributable to the adaptation and health of deep-sea organisms and has been revealed to be affected by many factors, especially the host species and diet. Here, we employed high-throughput sequencing of 16S rDNA to compare the gut microbial composition and diversity of two hadal amphipod species, Hirondellea gigas and Halice sp. MT-2017, which are predominant inhabitants in the Challenger Deep. Results showed that Tenericutes and Proteobacteria were the most abundant phyla, occupying more than 50% of total phyla, across all the specimens. At genus level, “Candidatus Hepatoplasma” was overall dominant, followed by Psychromonas in H. gigas and Psychrobacter in Halice sp. MT-2017 respectively. Notably, though two hadal amphipod species shared the same predominant symbiont of genus “Ca. Hepatoplasma”, they were classified into two different OTUs, which suggested that the colonization of symbionts were host-specific. Moreover, in terms of species relative abundance, alpha-diversity and beta-diversity, there was still a significant divergence of gut microbiota between two hadal amphipod species, which dwelled in the same extreme environment. Altogether, the results dropped a hint to the importance of symbiont genus of “Ca. Hepatoplasma” to amphipod survival in the hadal zone.

When designing the air nailer, in order to achieve the purpose of rapid analysis of design results, use of VB and APDL tools, secondary development of ANSYS, established a parametric CAE analysis module, template-based CAE analysis module, and profession CAE analysis module. And this three module is integrated into the air nail industrial design platform. As a result, designers can quickly and easily on the air nailer for CAE analysis. Meanwhile, in order to achieve in the 3D design of the system of the air nailer’s handle for comfort evaluation, an evaluation method is proposed. Analysis and demonstrated the principle of the method. Established a comfort evaluation model. On this basis, comfortable evaluation software is developed. Evaluation results generated by the software can be used as a reliable basis for revising shapes of handles.

Based on requirements of evaluation on comfort level of hand-held tool handles, this essay put forward an evaluation method as well as analyzed and proved its principle, established a corresponding evaluation mode, deliberated index and computation module for comfort level of tool handles. The authored also developed a set of software to evaluate comfort level of tool handles. With this software, one can efficiently evaluate the comfort level of tool handles and an evaluation report will be made automatically, which can serve as a reliable basis for revising shapes of handles.

Digital industrial design system combines the digital technology and industrial design process，and integrates the sub-systems of digital modeling, digital assemble, digital evaluation, digital manufacture, and digital information exchange. In order to let the industrial design work process and the design data flowing fluently around such sub-systems, Product Data Management (PDM) system is introduced here as the basic integration platform of the sub-systems to let the design process flow and design data flow working well. The product digital industrial design platform basic functions and its system structure is analyzed, and a general model and function frame of product data management system oriented to digital industrial design is presented in this paper to solve its characteristic data composition, integrative method of application software and industrial design flow in the process of the digital industrial design. Results about the working process integrative model, control process model, product class definition model, and the logical structure of the digital industrial design platform with the PDM system are also established. An application results for certain product development show that such digital industry design system can make the helps for the design work efficiency and the follow-up engineering design accuracy.

Computer Aided Industrial Design System is a key component of the product digital design platform. It is equally important to control its design process and manage its data and documents effectively to share the data generated in the whole design process. Based on the analysis of the basic functions of the product digital industrial design platform, this paper presents the general model and function frame of product data management system oriented to computer aided industrial design according to its data composition character, integrative method of application software and industrial design flow in the process of computer aided industrial design. In addition, with the industrial design of the pneumatic tools as the object, this paper presents the product data management system to achieve the system integration of computer aided industrial design, 3-D structure design and CAE analysis and the data share.

Radiotherapy may compromise the integration of implant and cause implant loss. Implant surface modifications have the possibility of promoting cell attachment, cell growth, and bone formation which ultimately enhance the osseointegration process. The present study aimed to investigate the effects of calcium phosphate nanocrystals on implant osseointegration in irradiated bone. Sixteen rabbits were randomly assigned into control and nano-CaP groups, receiving implants with dual acid-etched surface or dual acid-etched surface discretely deposited of nanoscale calcium-phosphate crystals, respectively. The left leg of all the rabbits received 15 Gy radiation, followed by implants placement one week after. Four animals in each group were sacrificed after 4 and 12 weeks, respectively. Implant stability quotient (ISQ), ratio of bone volume to total volume (BV/TV), bone growth rate, and bone-to-implant contact (BIC) were evaluated. The nano-CaP group showed significantly higher ISQ (week 12, P = 0.031) and bone growth rate (week 6, P = 0.021; week 9, P = 0.001) than that in control group. No significant differences in BV/TV and BIC were found between two groups. Titanium implant surface modified with CaP nanocrystals provides a potential alternative to improve bone healing around implant in irradiated bone., published_or_final_version

As an edible and medicinal plant, Coix seed is readily contaminated by more than one group of mycotoxins resulting in potential risk to human health. A reliable and sensitive method has been developed to determine seven mycotoxins (aflatoxins B1, B2, G1, G2, zearalenone, α-zearalenol, and β-zearalenol) simultaneously in 10 batches of Coix seed marketed in China. The method is based on a rapid ultrasound-assisted solid-liquid extraction (USLE) using methanol/water (80/20) followed by immunoaffinity column (IAC) clean-up, on-line photochemical derivatization (PCD), and high performance liquid chromatography coupled with fluorescence detection (HPLC-FLD). Careful optimization of extraction, clean-up, separation and detection conditions was accomplished to increase sample throughput and to attain rapid separation and sensitive detection. Method validation was performed by analyzing samples spiked at three different concentrations for the seven mycotoxins. Recoveries were from 73.5% to 107.3%, with relative standard deviations (RSDs) lower than 7.7%. The intra- and inter-day precisions, expressed as RSDs, were lower than 4% for all studied analytes. Limits of detection and quantification ranged from 0.01 to 50.2 μg kg(-1), and from 0.04 to 125.5 μg kg(-1), respectively, which were below the tolerance levels for mycotoxins set by the European Union. Samples that tested positive were further analyzed by HPLC tandem electrospray ionization mass spectrometry for confirmatory purposes. This is the first application of USLE-IAC-HPLC-PCD-FLD for detecting the occurrence of multi-class mycotoxins in Coix seed.

To investigate the impact of cover screw, resin embedment, and implant angulation on artifact of microcomputed tomography (micro-CT) scanning for implant.A total of twelve implants were randomly divided into 4 groups: (i) implant only; (ii) implant with cover screw; (iii) implant with resin embedment; and (iv) implants with cover screw and resin embedment. Implants angulation at 0°, 45°, and 90° were scanned by micro-CT. Images were assessed, and the ratio of artifact volume to total volume (AV/TV) was calculated. A multiple regression analysis in stepwise model was used to determine the significance of different factors. One-way ANOVA was performed to identify which combination of factors could minimize the artifact.In the regression analysis, implant angulation was identified as the best predictor for artifact among the factors (P 0.001). Resin embedment also had significant effect on artifact volume (P = 0.028), while cover screw had not (P 0.05). Non-embedded implants with the axis parallel to X-ray source of micro-CT produced minimal artifact.Implant angulation and resin embedment affected the artifact volume of micro-CT scanning for implant, while cover screw did not.

Objectives The aim of the study was to investigate the dose-dependent effect of radiation on dental implant stability and osseointegration using a series of quantitative analyses. Material and methods Six rabbits were randomly assigned to 15 and 30 Gy radiation groups. Each rabbit received radiation at the tibial and femoral metaphyseal region of left hind leg. The right leg was used as control. Implant surgery was performed on tibial and femoral metaphyses after 1 week. Totally 24 implants were inserted. The animals were killed at postoperative week four. Implant stability was measured using resonance frequency analysis. Ratio of bone volume to total volume (BV/TV), rate of bone growth, and bone-to-implant contact (BIC) were assessed using micro-computed tomography (micro-CT), fluorochrome labeling analysis, and histomorphometric analysis, respectively. Results After 4 weeks of healing, all implants were integrated (n = 6). Implant stability was significantly compromised by 15 Gy (P = 0.010) and 30 Gy (P = 0.025) of radiation. Radiation decreased BV/TV, and the significant effect was detected at the dose of 15 Gy (P = 0.008) and 30 Gy (P

Fumonisins are produced by the fungus Fusarium verticillioides, which are known to cause fatal diseases in some animals and humans. Here, we describe a sensitive, reproducible and reliable analytical method for the quantitative determination of fumonisins B(1) (FB(1)) and B(2) (FB(2)) in 112 spices and aromatic and medicinal herbs marketed in China. This method is based on high performance liquid chromatography and fluorescence detection (HPLC-FLD) coupled to a new on-line post-column derivatization using ortho-phthaldialdehyde with 2-mercaptoethanol and immunoaffinity column clean-up. Under the optimized experimental conditions, a complete separation of FB(1) and FB(2) was obtained using a Synergi C(18) column and a gradient elution at 0.8 mL min(-1) with methanol and 0.1 M phosphate buffer at pH 3.15. The limits of detection for FB(1) and FB(2) were both 40 μg kg(-1). Good recoveries were found for spiked samples with FB(1) and FB(2), ranging from 82.34% to 98.16% for FB(1) and from 72.58% to 97.10% for FB(2), with relative standard deviation (RSD)7.0%. 5 spices, 11 aromatic herbs and 96 medicinal herbs including 93 normal samples and 19 visibly moldy samples, which were spoiled artificially, were analyzed. The results showed that 8 (42.1%) visibly moldy samples and 8 (8.6%) normal samples were contaminated with FB(1) at mean contents of 129.0 and 165.9 μg kg(-1), and with FB(2) at 1745.0 and 256.8 μg kg(-1), respectively. Positive confirmation of detected samples was performed by liquid chromatography tandem electrospray ionization mass spectrometry (LC-ESI-MS/MS), using a triple quadrupole analyzer and operated in the multiple reaction monitoring mode.

Background Distraction osteogenesis is a controlled surgical procedure that initiates a regenerative process and uses mechanical strain to enhance the biological responses of the injured tissues to create new bone. To explore the effect of high-frequency mechanical traction on the expression of tissue inhibitor of matrix metalloproteinase-1 (TIMP-1), we compared the gene expression of TIMP-1 between continuous and intermittent distraction osteogenesis using a rabbit model of mandibular lengthening. Materials and methods Forty adult New Zealand white rabbits were randomly assigned to the intermittent and continuous distraction groups. A unilateral mandibular osteotomy was performed and a custom-designed manual-driven or auto-driven distractor was bridged over the osteotomy segments. Animals were sacrificed at day-6, day-10, day-14 and day-21 after osteotomy. Samples were examined with real-time polymerase chain reaction (PCR). Results Real-time PCR examination showed significantly higher mRNA levels of TIMP-1 under continuous distraction than that under intermittent distraction at day-6 and day-10. No significant differences were found at day-14 and day-21. Conclusion High-frequency traction provides a good mechanical environment for accelerating bone formation by up-regulating TIMP-1.