1. Analysis of DNA Damages of Gonadal Cells of Hemicentrotus pulcherrimus in Petroleum Hydrocarbons
- Author
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De Qi Xiong, Xin Lu Lv, Xiang Gao, Jun Song Han, and Ya Li Gao
- Subjects
Gel electrophoresis ,Transfer DNA ,biology ,DNA damage ,Cell ,General Medicine ,biology.organism_classification ,Molecular biology ,Hemicentrotus ,chemistry.chemical_compound ,medicine.anatomical_structure ,chemistry ,biology.animal ,medicine ,Test organism ,Sea urchin ,DNA - Abstract
The single cell gel electrophoresis (SCGE) is a rapid and sensitive procedure for measuring strand breaks in DNA. In the present study, sea urchin (Hemicentrotus pulcherrimus) was chosen as the test organism and SCGE was applied to assess DNA damage of its gonadal cells exposed to petroleum hydrocarbon. The gonadal cells of sea urchin had been seriously damaged above 50 mg/L of Water Accommodated Fractions (WAFs), whileas no damages occurred in the lower concentrations. There were good linear relationships between exposure days and DNA damage rate, percentage of DNA in the comet tail (%TDNA) as well as comet tail length (TL).
- Published
- 2014